• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.228-235
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• 2019

Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

• Journal of Korean Society of Forest Science
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• v.100 no.3
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• pp.432-440
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• 2011

The objective of this study was to investigate growth and fruiting characteristics (e.g., nut qualities) of chestnut (Castanea cerenata) after applying various fertilizer treatments at the cultivation site in Suncheon. Fertilizer treatments were designed as follow: liquid fertilizer, chemical fertilizer, organic fertilizer, and control. Both liquid and chemical fertilizer treatments provided the best growth in height and basal diameter. In addition, these two treatments were very effective for crown width of the trees between both east-west and north-south orientation. The liquid fertilizer treatment was effective on total length of the fruiting branch and length of the bearing to terminate part. Both liquid and chemical fertilizer treatments produced the longest length of basal to bearing part compared to the other two treatments. The liquid fertilizer treatment showed the most thickened basal diameter of the fruiting branch and the greatest diameter of above and below the bearing burr part. Elongation Index of the fruiting branch (EI) was the highest with liquid fertilizer treatment and the remaining four indices (Production Index of fruiting branch, PI; Ratio of Diameter between below and above bearing burr part, RD; Growth Index of fruiting branch diameter, GI; Thickness Index of fruiting branch or dormant branch, TI) were the highest with the chemical fertilizer treatment. Total number of produced branch per fruiting mother branch and number of small and weak branches per fruiting mother branch were highest on the control and liquid fertilizer treatment; however, all treatments produced similar numbers. The chemical and organic fertilizer treatments produced a high number of fruiting branches per fruiting mother branch, while organic and liquid fertilizer treatments produced a high number of burr per fruiting branch. The rate of commercializing on the basis of nut weight and quantity was higher on control (87.5%) than chemical fertilizer treatment (84.6%); however, the rate was even lower on liquid fertilizer treatment (84.3%) and organic fertilizer treatment (82.7%). The liquid fertilizer treatment showed the highest average of nut weight, while chemical fertilizer treatment showed the highest average number of fruiting burr. There was no significant difference in average number of normal nuts per burr among treatments. The yield per tree was high on chemical (8.2 kg) and liquid (8.0 kg) fertilizer treatments, but there was no significant difference among treatments. In the rate of nut grade on the basis of nut weight and quantity, the liquid fertilizer treatment, 43.5% and 34.3% more than large nut respectively, produced higher value chestnuts compared to other treatments.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.71-86
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• 2021

This study was conducted to find information on the university's image from words related to 'Korea National College of Agriculture and Fisheries (KNCAF)' in Naver News. For this purpose, word frequency analysis, TF-IDF evaluation and semantic network analysis were performed using web crawling technology. In word frequency analysis, 'agriculture', 'education', 'support', 'farmer', 'youth', 'university', 'business', 'rural', 'CEO' were important words. In the TF-IDF evaluation, the key words were 'farmer', 'dron', 'agricultural and livestock food department', 'Jeonbuk', 'young farmer', 'agriculture', 'Chonju', 'university', 'device', 'spreading'. In the semantic network analysis, the Bigrams showed high correlations in the order of 'youth' - 'farmer', 'digital' - 'agriculture', 'farming' - 'settlement', 'agriculture' - 'rural', 'digital' - 'turnover'. As a result of evaluating the importance of keywords as five central index, 'agriculture' ranked first. And the keywords in the second place of the centrality index were 'farmers' (Cc, Cb), 'education' (Cd, Cp) and 'future' (Ce). The sperman's rank correlation coefficient by centrality index showed the most similar rank between Degree centrality and Pagerank centrality. The KNCAF articles of Naver News were used as important words such as 'agriculture', 'education', 'support', 'farmer', 'youth' in terms of word frequency. However, in the evaluation including document frequency, the words such as 'farmer', 'dron', 'Ministry of Agriculture, Food and Rural Affairs', 'Jeonbuk', and 'young farmers' were found to be key words. The centrality analysis considering the network connectivity between words was suitable for evaluation by Cd and Cp. And the words with strong centrality were 'agriculture', 'education', 'future', 'farmer', 'digital', 'support', 'utilization'.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.89-104
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• 2021

Based on the TF-IDF weighted value that evaluates the importance of words that play a key role, the semantic network analysis(SNA) was conducted on the self-introduction letter of freshman at Korea National College of Agriculture and Fisheries(KNCAF) in 2020. The top three words calculated by TF-IDF weights were agriculture, mathematics, study (Q. 1), clubs, plants, friends (Q. 2), friends, clubs, opinions, (Q. 3), mushrooms, insects, and fathers (Q. 4). In the relationship between words, the words with high betweenness centrality are reason, high school, attending (Q. 1), garbage, high school, school (Q. 2), importance, misunderstanding, completion (Q.3), processing, feed, and farmhouse (Q. 4). The words with high degree centrality are high school, inquiry, grades (Q. 1), garbage, cleanup, class time (Q. 2), opinion, meetings, volunteer activities (Q.3), processing, space, and practice (Q. 4). The combination of words with high frequency of simultaneous appearances, that is, high correlation, appeared as 'certification - acquisition', 'problem - solution', 'science - life', and 'misunderstanding - concession'. In cluster analysis, the number of clusters obtained by the height of cluster dendrogram was 2(Q.1), 4(Q.2, 4) and 5(Q. 3). At this time, the cohesion in Cluster was high and the heterogeneity between Clusters was clearly shown.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.79-89
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• 2022

With the wide use of greenhouses, the working hours have been increasing inside the greenhouse for workers. In the closed ventilated greenhouse, the internal environment has less affected to external weather during making a suitable temperature for crop growth. Greenhouse workers are exposed to organic dust including soil dust, pollen, pesticide residues, microorganisms during tillage process, soil grading, fertilizing, and harvesting operations. Therefore, the health status and working environment exposed to workers should be considered inside the greenhouse. It is necessary to secure basic data on particulate matter (PM) concentrations in order to set up dust reduction and health safety plans. To understand the PM concentration of working environment in greenhouse, the PM concnentrations were monitored in the cut-rose and Hallabong greenhouses in terms of PM size, working type, and working period. Compare to no-work (move) period, a significant increase in PM concentration was found during tillage operation in Hallabong greenhouse by 4.94 times on TSP (total suspended particle), 2.71 times on PM-10 (particle size of 10 ㎛ or larger), and 1.53 times on PM-2.5, respectively. During pruning operation in cut-rose greenhouse, TSP concentration was 7.4 times higher and PM-10 concentration was 3.2 times higher than during no-work period. As a result of analysis of PM contribution ratio by particle sizes, it was shown that PM-10 constitute the largest percentage. There was a significant difference in the PM concentration between work and no-work periods, and the concentration of PM during work was significant higher (p < 0.001). It was found that workers were generally exposed to a high level of dust concentration from 2.5 ㎛ to 35.15 ㎛ during tillage operation.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.444-451
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• 2022

Recently, long-term cultivation is becoming more common with the increase in tomato hydroponics. In hydroponics, it is very important to supply an appropriate nutrient solution considering the nutrient and moisture requirements of crops, in terms of productivity, resource use, and environmental conservation. Since seasonal environmental changes appear severely in long-term cultivation, it is so critical to manage irrigation control considering these changes. Therefore, this study was carried out to investigate the effect of irrigation volume on growth and yield in tomato long-term cultivation using coir substrate. The irrigation volume was adjusted at 4 levels (high, medium high, medium low and low) by different irrigation frequency. Irrigation scheduling (frequency) was controlled based on solar radiation which measured by radiation sensor installed outside the greenhouse and performed whenever accumulated solar radiation energy reached set value. Set value of integrated solar radiation was changed by the growing season. The results revealed that the higher irrigation volume caused the higher drainage rate, which could prevent the EC of drainage from rising excessively. As the cultivation period elapsed, the EC of the drainage increased. And the lower irrigation volume supplied, the more the increase in EC of the drainage. Plant length was shorter in the low irrigation volume treatment compared to the other treatments. But irrigation volume did not affect the number of nodes and fruit clusters. The number of fruit settings was not significantly affected by the irrigation volume in general, but high irrigation volume significantly decreased fruit setting and yield of the 12-15th cluster developed during low temperature period. Blossom-end rot occurred early with a high incidence rate in the low irrigation volume treatment group. The highest weight fruits was obtained from the high irrigation treatment group, while the medium high treatment group had the highest total yield. As a result of the experiment, it could be confirmed the effect of irrigation amount on the nutrient and moisture stabilization in the root zone and yield, in addition to the importance of proper irrigation control when cultivating tomato plants hydroponically using coir substrate. Therefore, it is necessary to continue the research on this topic, as it is judged that the precise irrigation control algorithm based on root zone-information applied to the integrated environmental control system, will contribute to the improvement of crop productivity as well as the development of hydroponics control techniques.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.295-304
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• 2022

The purpose of this study is to detect the sorghum panicle using YOLOv5 based on RGB images acquired by a unmanned aerial vehicle (UAV) system. The high-resolution images acquired using the RGB camera mounted in the UAV on September 2, 2022 were split into 512×512 size for YOLOv5 analysis. Sorghum panicles were labeled as bounding boxes in the split image. 2,000images of 512×512 size were divided at a ratio of 6:2:2 and used to train, validate, and test the YOLOv5 model, respectively. When learning with YOLOv5s, which has the fewest parameters among YOLOv5 models, sorghum panicles were detected with mAP@50=0.845. In YOLOv5m with more parameters, sorghum panicles could be detected with mAP@50=0.844. Although the performance of the two models is similar, YOLOv5s ( 4 hours 35 minutes) has a faster training time than YOLOv5m (5 hours 15 minutes). Therefore, in terms of time cost, developing the YOLOv5s model was considered more efficient for detecting sorghum panicles. As an important step in predicting sorghum yield, a technique for detecting sorghum panicles using high-resolution RGB images and the YOLOv5 model was presented.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.300-310
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• 2022

The objective of this experiment was to investigate the effect of drip irrigation volume on tomatoes (Solanum lycopersicum L.) grown in a greenhouse using perlite medium. Plants were treated by three different irrigation treatment I₀, I₂₅, and I₅₀ (where irrigation volume of I₂₅ and I₅₀ was 25% and 50% higher than I₀, having limited or no leaching). Growth characteristics of plants, yield and water use efficiency were measured. The result showed that plant height, leaf length and leaf width were lowest in the I₀ treated plants. However, these parameters were not statistically significant differences between the plants that were grown in the I₂₅ and I₅₀ treatment. Soluble solids content, acidity and dry matter of 111th, 132nd, and 143rd days harvested tomato were higher in the plants irrigated with lowest volume (I₀) than the higher volume (I₂₅ or I₅₀). In addition, water content was lower in the 111th and 132nd days of harvested tomatoes from the I₀ treatment. The number of big-size tomatoes (>180 g) was significantly higher in the I₂₅ irrigated plants. There was no significant difference in the total number of harvested fruits among the treatments. The average fruit weight and total yield of harvested tomatoes were lowest in the I₀ treated plants. The water consumption of tomato was not significantly different amongst the treatments but water use efficiency was lowest in the I₀ treatment. Principal component analysis revealed that total soluble solid and acidity of tomato showed a positive correlation between each other. These results suggest that I₂₅ was the optimum irrigation treatment for tomato based on its measured growth characteristics, yield and water use efficiency.

• Journal of Practical Agriculture & Fisheries Research
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• v.10 no.1
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• pp.43-58
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• 2008

This research was conducted to determine the alternative tool of chemical spray for rice cultivation using the unmanned helicopter(Yamaha, R-Max Type 2G-remote controlled system) at farmer's field in Korea. The unmanned helicopter tested was introduced form Japan. In Korea the application of chemicals by machine sprayer for pest management in rice cultivation has been ordinarily used at the farmer's level. However, it involved a relatively high cost and laborious for the small scale of cultivation per farm household. Farm population has been highly decreased to 7.5% in 2002 and the population is expected to rapidly reduce by 3.5% in 2012. In Japan, pest control depending on unmanned helicopter has been increased by leaps and bounds. This was due in part to the materialization of the low-cost production technology under agricultural policy and demand environmentally friendly farm products. The practicability of the unmanned helicopter in terms of super efficiency and effectiveness has been proven, and the farmers have understood that the unmanned helicopter is indispensable in the future farming system that they visualized. Also, the unmanned helicopter has been applied to rice, wheat, soybean, vegetables, fruit trees, pine trees for spraying chemicals and/or fertilizers in Japan Effect of disease control by unmanned helicopter was partially approved against rice blast and sheath blight. However, the result was not satisfactory due to the weather conditions and cultural practices. The spray density was also determined in this experiment at 0, 15, 30, and 60cm height from the paddy soil surface and there was 968 spots at 0cm, 1,560 spots at 15cm, 1,923 spots at 30cm, and 2,999 spots at 60cm height. However, no significant difference was found among the treatments. At the same time, there was no phytotoxicity observed under the chemical stray using this unmanned helicopter, nor the rice plant itself was damaged by the wind during the operation.

• Research in Plant Disease
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• v.28 no.4
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• pp.221-230
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• 2022

The fire blight caused by Erwinia amylovora (Ea) was first reported in 2015 in Korea, and the disease has rapidly spread to 22 regions until 2021. In Korea, all host plants in the apple and pear orchards where fire blight occurred should be eliminated and buried by the Plant Protection Act. To prevent the spread of the disease, all burial sites were prohibited from planting the new host plants for the next three years. To confirm the eradication efficiency of Ea and the reoccurrence of fire blight, the surveillance facilities were established on three burial sites from 2019 to 2020 in Anseong-si, Gyeonggi-do, and Chungju-si, Chungcheongbuk-do. As host plants, five apple trees of fire blight-susceptible cultivar 'Fuji', were planted in each facility. All facilities were enclosed with fences and nets and equipped with two CCTVs, motion sensors, and several other sensors for recording weather conditions to monitor the environment of the sentinel plants in real-time. The sentinel plants were checked for the reoccurrence of fire blight routinely. Suspicious plant parts were collected and analyzed for Ea detection by loop-mediated isothermal amplification polymerase chain reaction and conventional polymerase chain reaction. Until November 2022, Ea has not been detected in all sentinel plants. These results might support that the burial control of infected plants in soil works efficiently to remove Ea and support the possibility to shorten the prohibition period of host plant establishment in the burial sites.