• Korean journal of applied entomology
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• v.56 no.3
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• pp.275-282
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• 2017

The purpose of this study was to analyze the characteristics of hemocytes in the hemolymph of the larvae of Pentodon quadridens bidentulus and the characteristics of the hemocytes responsible for cellular immunity during pathogen infection. Granulocytes, plasmatocytes, oenocytoids, spherulocytes, prohemocytes and adipohemocytes were found in the circulating hemocytes. Among them, granulocyte were observed as cells responsible for immunological phagocytosis during entry of foreign substances. In particular, it was observed that the most active phagocytic action occurred within 12 hours in vivo, and that after 24 hours, the immune activation was reduced and converted to a normal state. Plasmatocytes were occasionally observed as immunological response, but the remaining hemocytes were not related to immunological activation.

• Applied Microscopy
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• v.10 no.1_2
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• pp.1-6
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• 1980

The secretory granul cells in the midgut epithelium of Blattella germanica L. were observed by the electron microscope. These secretory granul cells contain many electron dense granules, and granules are about $200{\AA}$ in diameter respectively. It is easy to distinguish 3 different types of granul cells based on their shapes, location, and staining intensity: 1) The light secretory granul cells and their nucleus are both round form and a number of mitochondria, vacuoles, and other cell organelles appear in the cytoplasm. 2) The other kind of light secretory granul cells are small and oval form but ceil organelles are not well developed in the cytoplasm. This granul cell is surrounded by a few regenerative cells ('nidi'). 3) Dark secretory granul cells are cone shaped, well stained, and endoplasmic reticulum, ribosomes, and a lot of secretory granules are found in the cytoplasm. They are all located in the basal portion of the midgut epithelium.

• Applied Microscopy
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• v.34 no.3
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• pp.199-209
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• 2004

The migut epitheluim of the last instar larva in the mosquito larvae, Anopheles sinensis was observed with electron microscopes. The midgut epitheluim of the mosquito larva is composed of a single-layered columnar absorptive cells, regenerative cells and secretory granular cells. The free surface of the columnar absorptive cells has a regular array of microvilli 'brush border', while cell membranes close to the basal lamina are extrmely infolded and a lot of mitochondria are concentrated in those processes. The columnar absorptive cells also contain cell organelles expected to be found in absorptive cell. Midgut regenerative cells which are positioned basally in the epithelium form the groups, which are called 'nidi', composed of 1 or $2{\sim}3$ cells, they show darker appearance than the columnar absoptive cells. The secretory granular cells contain numerous electron dense granules, $200{\sim}400$ nm in diameter. The cone shaped secretory granular cells are located in the basal portion of the midgut epitheluim. The epithelium is surrounded by the subepithelial space and muscle bundles. The subepithelial space, which is filled with fibrous connective tissue, is innervated by many axon cells and tracheoles.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.29 no.4
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• pp.166-171
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• 2018

The effect of bisphenol S (BPS) on the viability and production of reactive oxygen species (ROS) was studied in boar sperm and ovarian granulosa cells. Boar semen was incubated in Beltsville thawing solution with either 0 or $5{\mu}M$ BPS for 3 and 6 h. The viability of sperm was analyzed by SYBR14/PI doubling staining, and production of ROS was detected. Ovarian granulosa cells were also treated with BPS for 24, 48, and 72 h. Then, cell viability (0, 5, 10, and $20{\mu}M$) and ROS production (only 0 and $5{\mu}M$ BPS) were assessed. The results showed that, BPS decreased sperm viability at 3 and 6 h, and that BPS increased ROS production (p<0.05). Also, BPS reduced the viability of ovarian granulosa cells (p<0.05), and stimulated ROS production (p<0.05). These results suggest that BPS damages sperm activation and ovarian granulosa cells in the reproductive system.

• Applied Microscopy
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• v.37 no.2
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• pp.83-91
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• 2007

The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.

• Applied Microscopy
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• v.32 no.3
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• pp.213-222
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• 2002

Histology and ultrastructure of the mantle epidermis in the ark shell, Scapharca broughtonii are described using light and electron microscopy. The mantle of the ark shell is composed of outer epidermis, connective tissue and inner epidermis. Both epidermis are simple and consists of supporting cells, ciliated cells and secretory cells. Connective tissue is composed of mainly collagen and muscle fibers. The supporting cells in the inner epidermis are usually columnar and covered with microvilli. The ciliated cell have cilia and microvilli on the free surface, and numerous tubular mitochondria are observed in the apical cytoplasm. Secretory cells are mainly observed in the outer epidermis, and it can be divided into four types of A, B, C and D with morphological features of the secretory granules. Type A cells of mucous cell are found in the marginal and central mantle. And these cells contains numerous secretory granules of non-bounded and low electron density. Type B cells contains numerous rough endoplasmic reticula, well-developed Golgi complex and secretory granules of membrane-bounded and high electron density. Secretory granules of type C cells are divided into fibrous core layer and homogeneous peripheral layer. Type D cells are found in the outer epidermis of the central and umbonal mantle. And secretory granules of these cells are divided into homogeneous core layer and granular peripheral layer. This results suggest that the outer and inner epidermis of the mantle are related with shell formation and cleaning of the mantle cavity, respectively.

• The Korean Journal of Zoology
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• v.21 no.1
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• pp.29-39
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• 1978

The authors observed the ultrastructure of the mucous glandular epithelial cells in the amphibian skin by mean of electron microscope. The specimens from the experimental animals were fixed in 2.5% glutaraldehyde-oaraformaldehyde fixative in phosphate buffer at pH 7.2 prior to fixation in 1% osmium tetroxide, dehydrated in graded ethanol and acetone, embedded in Epon 812 mixture, and sectioned with Sorvall MT-2 ultramicrotome. The ultrasections were contrasted with uranyl acetate and lead citrate and observed with a JEOL-100B electron microscope. The results were as follows: 1. The cutaneous mucous glands in amphibia consisted of the glandular epithelial and the myoepithelial cells. 2. Several different cells in ultrastructure were observed in the mucous glandular epithelium of the adult amphibian skin. a. The dark and the light cells were observed in Hynobius leechi. b. The mitochondria-rich and the round secretory granule-containing cells were observed in Bombina orientalis. c. The round secretory granule-containing and the foam-like granule mass-containing cells were observed in Kaloula tornieri. d. The cutaneous mucous gland of Rana nigromaculata were divided into two types: A and B-type glands. In the A-type mucous gland, the mitochondria-rich and the round secretory granule-containing cells and in the B-type mucous gland, the mitochondria-rich, the secretory granule-containing and the ER-rich cells were observed. 3. Based upon the above findings, the authors infer that the mucous granular epithelium of the amphibian skin consists of the mitochondria-rich undifferentiated, the secetory granule-containing and mature, and the ER-rich evacuated cells.

• The Korean Journal of Cytopathology
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• v.7 no.1
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• pp.31-37
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• 1996

It is well known that fine needle aspiration biopsy(FNAB) is very useful and has a high accuracy rate in the diagnosis of renal neoplasms. Although there is some indecision to perform the FNAB for a rare possibility of tumor seeding along the biopsy needle tract, it tends to be used increasingly. As in the cytologic diagnosis of metastatic lesion through-out the body, renal cell carcinoma should nearly always be considered in the differential diagnosis, the precise understanding of cytologic features of renal cell carcinoma with various cell types and architectural patterns is necessarily required. In this report, we present three cases of primary renal cell tumors, two of renal cell carcinomas and one of oncocytoma, preponderantly emphasizing the cytologic differential points in the FNAB specimen.

• Proceedings of the KAIS Fall Conference
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• 2011.05a
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• pp.64-66
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• 2011

최근 웰빙에 대한 사회적 관심이 증가하면서 다양한 기능성 식품들이 앞다퉈 개발되고 있다. 본 논문에서는 제주산 감태에서 추출한 후코이단이라는 해조류 추출물 성분을 이용하여 기능성 차를 개발하였다. 후코이단은 암의 치료에 효능이 있을 뿐만 아니라 뇌의 혈액 개선의 효과가 있으며, 면역력 증강작용, 혈액응고 저지 작용, 비만억재와 당뇨 치료에도 효과가 있다. 후코이단은 분말가루, 액상형, 과립형 등으로 시판되고 있다. 하지만 홍보가 미약하여 많이 알려져 있지 않고 있으며 또한 항암작용에 대한 기능만이 부각되어 있어 오히려 소비확대가 이루어지지 않고 있다. 본 논문에서는 일반적으로 사람들이 쉽게 접할 수 있는 식품을 통해 후코이단의 섭취를 권장할 수 있는 올레차를 개발하였으며, 후코이단 특유의 비릿한 향을 중화하기 위해 제주의 유채꽃과 꿀을 이용하였다.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.10 no.2
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• pp.159-165
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• 2000

This study was investigated the environment of waste shells such as oyster, cockle and paphia on southern shore in korea and established the recycling method to prevent the environmental pollution, etc. The waste shells were reclaimed at public shore illegally or leaved on the surroundings of shore. The origin mechanism, XRD and TG-DTA analyses were performed to effective recycling of waste shells, and the optimal recycling method was preparation of the calcium carbonate. In this work, calcium carbonate and lime fertilizer of granular shape were prepared using the waste shell.