• Title/Summary/Keyword: 고온스트레스

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Assessment of High Temperature Impacts on Early Growth of Garlic Plant (Allium sativum L.) through Monitoring of Photosystem II Activities (광계II 활성 분석을 통한 마늘의 생육초기 고온 스트레스의 영향 평가)

  • Oh, Soonja;Moon, Kyung Hwan;Koh, Seok Chan
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.829-838
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    • 2015
  • Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures ($10-30^{\circ}C$) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of $15-25^{\circ}C$. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at $20^{\circ}C$, compared to either below or above $20^{\circ}C$. $F_v/F_o$ and $F_v/F_m$ values were highest at $15-20^{\circ}C$, and decreased above $25^{\circ}C$. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields $F_i$ and $F_P$ decreased considerably above $25^{\circ}C$, with the increase of $F_k$ and $W_k$. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in $20-25^{\circ}C$, and decreased significantly in $30^{\circ}C$. These results indicate that a growth temperature of $30^{\circ}C$ inhibits early growth of garlic and that it is desirable to culture garlic plants near $20^{\circ}C$. Fluorescence parameters such a $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, and $PI_{abs}$ were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.

A Study on Development of Freshwater Fish Shelter and Evaluation of Water Quality for the Reduction of Thermal Stress in Shallow Pond (얕은 연못에서 담수 어류의 열성 스트레스 저감을 위한 피난처 개발 및 수질환경 조사 연구)

  • Lee, Saeromi;Ahn, Chang Hyuk;Joo, Jin Chul;Song, Ho Myeon;Park, Jae Roh
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.12
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    • pp.828-836
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    • 2014
  • The purpose of this study evaluates the water quality of artificial deep pool (ADP) during the late spring and summer. we monitored the water quality, as pH, dissolved oxygen (DO), temperature and conductivity at two stations (St. 1. open water, St. 2. in the ADP). The water quality in the ADP is very stable, and temperature and DO are lower than the open water (average value; temperature $1.4{\sim}3.2^{\circ}C$, DO 2.4~3.6 mg/L). In particular, daily variation of temperature in the open water showed above $4^{\circ}C$, but ADP remained stable. The water quality was analyzed using two-way analysis of variance (ANOVA). The results of the analysis showed difference about temperature, pH, DO, conductivity (two-way ANOVA, p<0.05). The ADP has created an aqua environment in thermal and DO gradients by depth. About 1.2 m, Temperature and DO were sharply decreased. The Rhodeus uyekii is dominant species in pond of this study. The ADP offers optimum water temperature ($16.5{\sim}18.5^{\circ}C$) to the Rhodeus uyekii about spawning. Consequently, the ADP offers stable habitat than open water for fish and aquatic organisms during the summer. It is also a good shelter for fish from a thermal stress.

An Analysis of Thermal Comforts for Pedestrians by WBGT Measurement on the Urban Street Greens (도심 가로 녹음의 습구흑구온도(WBGT) 측정을 통한 보행자 열쾌적성 효과 분석)

  • Ahn, Tong-Mahn;Lee, Jae-Won;Kim, Bo-Ram;Yoon, Ho-Seon;Son, Seung-Woo;Choi, Yoo;Lee, Na-Rae;Lee, Ji-Young;Kim, Hae-Ryung
    • Journal of the Korean Institute of Landscape Architecture
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    • v.41 no.3
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    • pp.22-30
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    • 2013
  • This study aims to measure the thermal comfort effects of urban street trees. As the usual dry bulb air temperature does not indicate properly how the average pedestrian feels the heat of a typical summer day under the strong sunshine, we adopted the Wet Bulb Globe Temperature(WBGT). WBGT involves black globe temperature to measure the direct radiation of sun beams on our bodies, for example our heads. We measured temperatures on very sunny and hot summer days, August 3, 4, and 7, 2012, on the urban streets of Seoul, Korea. Wet bulb, globe, and dry bulb temperatures were measured under direct sunlight from 1 O'clock to 5 O'clock pm. Globe and dry bulb temperatures were measured under street tree shades nearby during the same hours. Then the WBGTs were calculated with the formulae, one for sunny outdoor spaces, and the other for shaded outdoor spaces or indoor. The results are compared with the Korean Standards Association(KS A ISO 7243). The major findings were: 1) On very sunny and hot summer days in Seoul, street tree shades lower the WBGT about 1 to 4 degrees, 2) during the hours of 3 and 4 O'clock in the afternoon, the WBGT under the tree shades are about 3 to 4 degrees lower compared to those under sunshines(approx. 29 to 32 degrees respectively), 3) This difference makes a major thermal comfort for urban pedestrians because senior citizens or weak persons are recommended to move indoor, and even healthy people are recommended stop outdoor sports and take rests in the shades when WBGT is about 32. On the other hand, if the WBGT is around 29, or 3 degrees lower, slower walking, light works or sports are allowable, 4) On site questionnaire survey confirms the thermal comforts under the tree shades, and we even could not get survey subjects on the sunny parts of the sidewalks, 5) We strongly recommend change of guidelines for urban street trees from "one row of street trees on 6m~8m intervals" to "street trees to make continuous shades".

Antioxidant and Antigenotoxic Effects of Sansuyu Fruit (Corni fructus) Extracted with Water at Different Temperatures (추출 온도에 따른 산수유의 항산화 활성 및 항유전독성 효과 비교)

  • Lee, Min-Hee;Kim, Jung-Mi;Park, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.149-155
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    • 2011
  • The objective of this study was to evaluate the antioxidant and antigenotoxic activities of sansuyu fruit (Corni fructus, CF) at temperatures of $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$ using a water extraction method. Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity, and ORAC (Oxygen radical absorbance capacity) values were determined. Also the antigenotoxicity of CF was determined by measuring inhibitory effects of $H_2O_2$ induced DNA damage in human leukocytes using the comet assay. The TPC in the CF extracts was 4.2, 4.6, and 5.5 g/100 g GAE in $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$, respectively. The DPPH RSA of the CF extracts increased in a dose-dependent manner over the range of $50\sim1000\;{\mu}g$/mL in all temperatures and the $SC_{50}$ of DPPH RSA of the CF extracts were not significantly different at different extraction temperatures. The $SC_{50}$ of SOD-like was the highest in CF extracted at $25^{\circ}C$ (1.1 mg/mL) followed by $90^{\circ}C$ (1.2 mg/mL) and $50^{\circ}C$ (1.3 mg/mL). The ORAC values of the CF extracts were not significantly different in low concentration ($10\;{\mu}M$/mL) and was in order of $25^{\circ}C$ ($5.7\;{\mu}M$ TE)< $90^{\circ}C$ ($6.2\;{\mu}M$ TE)< $50^{\circ}C$ ($8.5\;{\mu}M$ TE) in high concentration ($50\;{\mu}M$/mL). $200\;{\mu}M$ $H_2O_2$ induced DNA damages in human leukocytes were significantly reduced by the pretreatment with the CF extracts. These results suggest that sansuyu fruit (Corni fructus) can be used as a natural source for antioxidant activities and as antigenotoxic agents regardless of the water extraction temperature.

A Study on the Relationship Between Radial Growth and Climate Factors by Regions in Korean Pine (Pinus koraiensis) (지역별 잣나무의 연륜생장과 기후인자와의 관계 연구)

  • Lee, Sangtae;Bae, Sang-Won;Jang, Seok Chang;Hwang, Jaehong;Chung, Junmo;Kim, Hyun-Seop
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.733-739
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    • 2009
  • This study was carried out to analyze the relationship between major climatic factors (monthly temperature, precipitation) and radial growth of Korean Pine (Pinus koraiensis) in Inje-gun(Mt. Seorak), Sanchung-gun and Namwon-si(Mt. Jiri). Radial growth measurements were crossdated and correlated with climate variables. The climatic factors used were monthly mean temperature and monthly precipitation from August of previous year to September of the current year. In Inje-gun, radial growth was negatively correlated with monthly mean temperature in April, May, June, and August of the current year and was positively correlated with precipitation in August, October of the previous year and in April, August of the current year. Sanchung-gun showed a negative correlation with monthly mean temperature in September of the previous year and August of the current year but positive correlation in January of the current year. In contrast, precipitation in September of the previous year and August of the current year showed a positive influence. The results suggest that high temperature together with low precipitation may thus cause water stress and thereby limit radial growth in this region. In Namwon-si, the climategrowth relationships show that radial growth was negatively correlated with monthly mean temperature in August, October of the previous year and June of the current year. But August of the previous year and May, August of the current year in precipitation was positively correlated with radial growth. In the study areas, precipitation plays an important role for the radial growth of Kroean Pine (Pinus koraiensis). The results suggest that precipitation is important in the radial growth because water deficit in trees would take place in response to high temperature in the study area. Also there was some differences with study areas in the effects of both climatic variables probably as a result of different stand structure and geographical conditions and micro-climate.

Effects of Environmental Factors on Growth and Nitrogen Fixation Activity of Autumn Olive (Elaeognus umbellata) Seedlings (보리수나무 유식물의 생장과 질소고정 활성에 대한 환경요인의 영향)

  • 송승달
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.387-394
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    • 1994
  • Effects of environmental factors of light, temperature, nitrogen sources and water stress were analyzed quantitatively on the nodule formation and nitrogen fixation activity of autumn olive plant (Elaeagnu$ umbellala Thunb.) during the seedling growth. Seedlings showed the maximum nitrogenase activity of $72.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ in the early nodulation stage. The relative growth rate and T/R ratio changed from $1.60%{\cdot}d^{-1}$ and 1.12 in the earlier stage to $3.75%{\cdot}d^{-1}$ and 2.31 in the later stage, respectively. light conditions of 20-25, 1015 and 4-6% resulted in decreases of 41, 54 and 71% of the nitrogenase activity, respectively. Nodules incubated in 15, 20, 25 and $30^{\circ}C$ showed the activities of 5.4, 24.7, 51.6 and $58.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ respectively. Pretreatment with low temperature ($15^{\circ}C$) followed incubation at $30^{\circ}C$ attained higher nitrogenase activity ($66.5\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$) than that with higher temperature ($35^{\circ}C$). The oxygen pressure above 16 kPa is necessary for saturation of the nodule activity, but the activity was inhibited severely by physical impact such as the exision or isolation of nodules from the root. The relative activities of early nodules grown in pH 5.5, 6.5 and 8.0 were 89, 100 and 40% and those grown in 1 and 3 mM of $NO_3\;and\;NH_4$ were 6, 1 and 68, 50%, respectively. Watering levels of 20, 50 and 100 mL during the seedling growth resulted in 35, 120 and 8 mg of nodule formation and 33.6, 58.4 and $8.4\;\mu\textrm{M}\;C_2H_4{\cdot}g\;fr\;wt\;nodule^{-1}{\cdot}h^{-1}$ of the nitrogenase activity, respectively. Water stress with 86% decrease of soil water content caused temporary wilting point of leaf and a complete disappearance of nitrogenase activity of nodules, though the water content and transpiration rate in plant were reduced to 90 and 53%, respectively.tively.

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Ethanol Induced Leucocytic and Hepatic DNA Strand Breaks Are Prevented by Styela clava and Styela plicata Supplementation in Male SD Rats (알코올로 인한 흰쥐의 백혈구 및 간 DNA 손상에 미치는 미더덕과 오만둥이 분말의 보충섭취 효과)

  • Kim, Jung-Mi;Park, Hae-Ryoung;Lee, Seung-Cheol;Park, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.10
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    • pp.1271-1278
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    • 2007
  • In this study, the ability of Styela clava or Styela plicata to reduce ethanol-induced hepatotoxicity and hepatic and leucocytic DNA damages was evaluated. Twenty four male SD rats were given 25% ethanol containing water (ad lib, p.o.) and divided into 3 groups; ethanol treated control group (EtOH), ethano1+3% S. clava (EtOH+SC), and ethano1+3% S. plicata (EtOH+SP). After 6 weeks, the supplementation of S. clava reduced the plasma ALT, ALP and LDH activities significantly (p<0.05), while S. plicata induced significant decrease in the plasma LDH activity only. The comet assay was employed to quantify the alcohol-induced DNA damage in rat hepatocytes and leucocytes. A significant protective effect on hepatic and leucocytic DNA damages was observed in S. clava or S. plicata supplemented groups compared to the EtOH control group. The hepatic DNA damage was correlated positively with plasma ALP and LDH activities. These results demonstrated that S. clava or S. plicata supplementation protected alcohol-induced hepatic and leucocytic DNA damage.

Risk Assessment of Pine Tree Dieback in Sogwang-Ri, Uljin (울진 소광리 금강소나무 고사발생 특성 분석 및 위험지역 평가)

  • Kim, Eun-Sook;Lee, Bora;Kim, Jaebeom;Cho, Nanghyun;Lim, Jong-Hwan
    • Journal of Korean Society of Forest Science
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    • v.109 no.3
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    • pp.259-270
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    • 2020
  • Extreme weather events, such as heat and drought, have occurred frequently over the past two decades. This has led to continuous reports of cases of forest damage due to physiological stress, not pest damage. In 2014, pine trees were collectively damaged in the forest genetic resources reserve of Sogwang-ri, Uljin, South Korea. An investigation was launched to determine the causes of the dieback, so that a forest management plan could be prepared to deal with the current dieback, and to prevent future damage. This study aimedto 1) understand the topographic and structural characteristics of the area which experienced pine tree dieback, 2) identify the main causes of the dieback, and 3) predict future risk areas through the use of machine-learning techniques. A model for identifying risk areas was developed using 14 explanatory variables, including location, elevation, slope, and age class. When three machine-learning techniques-Decision Tree, Random Forest (RF), and Support Vector Machine (SVM) were applied to the model, RF and SVM showed higher predictability scores, with accuracies over 93%. Our analysis of the variable set showed that the topographical areas most vulnerable to pine dieback were those with high altitudes, high daily solar radiation, and limited water availability. We also found that, when it came to forest stand characteristics, pine trees with high vertical stand densities (5-15 m high) and higher age classes experienced a higher risk of dieback. The RF and SVM models predicted that 9.5% or 115 ha of the Geumgang Pine Forest are at high risk for pine dieback. Our study suggests the need for further investigation into the vulnerable areas of the Geumgang Pine Forest, and also for climate change adaptive forest management steps to protect those areas which remain undamaged.