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Anti-Inflammatory and Whitening Effect of the Lyophilized Powder of Oriental Plant Extracts Fermented with Streptococcus thermophilus (Streptococcus thermophilus로 발효한 한약재 발효분말의 항염증 및 미백 효과)

  • Choi, Hwa-Jung;Lee, Jung-Hee;Yun, Mi-Young;Lee, Jae-Sug
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.2
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    • pp.159-164
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    • 2015
  • To identify new anti-inflammatory and whitening material, this study investigated the whitening and anti-inflammatory effects of the lyophilized powder from 6 oriental plant extracts (OPE; Citrus junos Tanaka, Mori cortex Radicis, Schisandra chinensis Baillon, Coix lachrymajobi var. mayuen, Angelica gigas NAKAI, and Sophora japonica L.) fermented with Streptococcus thermophilus by assessment of cytotoxicity on human dermal fibroblast, inhibitory effect of nitric oxide (NO) prodction, tyrosinase activity and melanin formation. The OPE was fermented with Streptococcus thermophilus at $37^{\circ}C$ for 2 days and the lyophilized powder was manufactured by freezing-dryer. OPE didn't show cytotoxicity at concentration of $500{\mu}g/mL$ using a cytopathic effect reduction method. OPE also exhibited inhibitory effect on nitric oxide (NO) prodction by Griess reagent system. Furthermore, OPE showed inhibitory effect on tyrosinase activity with dose dependent manner, and exhibited significant inhibition of melanin formation by measurement of melanin from culture media (p < 0.05). From these results, 6 OPE extracts showed anti-inflammatory and whitening effect and may be used as an active ingredient for cosmetics.

Evaluating Physical Characteristics of Raindrop in Anseong, Gyeonggi Province (강우입자의 물리적 특성평가: 경기도 안성시 지역을 사례로)

  • KIM, Jin Kwan;YANG, Dong Yoon;KIM, Min Seok
    • Journal of The Geomorphological Association of Korea
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    • v.17 no.1
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    • pp.49-57
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    • 2010
  • To evaluate physical characteristics of open rainfall in Korea, terminal velocity of raindrop and drop size distributions (DSD) were continuously measured using by laser-optical disdrometer around Gosam reservoir, Anseong-si, Gyeonggi-do during three rainfall events from 2008 to 2009. The relationships between kinetic energies (KE, Jm-2mm-1; KER, Jm-2h-1) and rainfall intensity were obtained, respectively. Moreover, we compared the rainfall intensity from a disdrometer with the rainfall intensity from a tipping bucket raingauge to transform the kinetic energy of rainfall using the data from a tipping bucket raingauge. Therefore, the established relationships between kinetic energies (KE and KER) and rainfall intensity could be a useful model to consider the kinetic energy of raindrop using the rainfall intensity below 40mmh-1 of max 5-min rainfall intensity in the middle of South Korea. However, to better examine the relationship between kinetic energy and rainfall intensity, further measurement will be required.

Assessment of the Contribution of Weather, Vegetation, Land Use Change for Agricultural Reservoir and Stream Watershed using the SLURP model (I) - Preparation of Input Data for the Model - (SLURP 모형을 이용한 기후, 식생, 토지이용변화가 농업용 저수지유역과 하천유역에 미치는 기여도 평가(I) - 모형의 입력자료 구축 -)

  • Park, Geun-Ae;Lee, Yong-Jun;Shin, Hyung-Jin;Kim, Seong-Joon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.2B
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    • pp.107-120
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    • 2010
  • The effect of potential future climate change on the inflow of agricultural reservoir and its impact to downstream streamflow by reservoir operation for paddy irrigation water was assessed using the SLURP (semi-distributed land use-based runoff process), a physically based hydrological model. The fundamental input data (elevation, meteorological data, land use, soil, vegetation) was collected to calibrate and validate of the SLURP model for a 366.5 $km^2$ watershed including two agricultural reservoirs (Geumgwang and Gosam) located in Anseongcheon watershed. Then, the CCCma CGCM2 data by SRES (special report on emissions scenarios) A2 and B2 scenarios of the IPCC (intergovernmental panel on climate change) was used to assess the future potential climate change. The future weather data for the year, m ms, m5ms and 2amms was downscaled by Change Factor method through bias-correction using 3m years (1977-2006) weather data of 3 meteorological stations of the watershed. In addition, the future land uses were predicted by modified CA (cellular automata)-Markov technique using the time series land use data fromFactosat images. Also the future vegetation cover information was predicted and considered by the linear regression between monthly NDVI (normalized difference vegetation index) from NOAA AVHRR images and monthly mean temperature using eight years (1998-2006) data.

Controlling effect of environmentally friendly organic materials on the black rice bug, Scotinophara lurida(Hemiptera: Pentatomidae), depending on paddy flooding (논 담수 여부에 따른 유기농업자재의 먹노린재 방제 효과)

  • You Kyoung Lee;Nak-Jung Choi;Ju-Rak Lim;Jun-Yeol Choi;Bo Yoon Seo
    • Korean Journal of Environmental Biology
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    • v.41 no.4
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    • pp.463-472
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    • 2023
  • The insecticidal activities of 27 different commercial products with environmentally friendly organic material(EFOM) against Scotinophara lurida, a major rice pest, were evaluated in the laboratory using spraying methods on plants and insects. Seven plant-derived organic farming materials (EFOM-8, -10, -12, -13, -19, -20, and -26) with high insecticidal effects when sprayed directly on the insect's body rather than on the plant were selected. In the indoor rice pot test, all 7 EFOMs showed an insecticidal rate of over 73.3% under flooding conditions. Notably, EFOM-13 and EFOM-20 demonstrated much higher insecticidal rates, ranging from 1.5 to 1.8 times, in flooding conditions compared to drained conditions. In the semi-paddy field test, EFOM-10 (80% garlic extract), EFOM-13 (62% neem extract), and EFOM-26 (70% sophora extract+28% ethyl alcohol+2% pyrethrum extract) exhibited a higher control value of 88.9% in the irrigated paddy on the 7th day, surpassing the control values in the drained paddy by 1.4 to 1.9 times. The control value in the irrigated rice paddy field sprayed with EFOM-10 reached 86.2% on the 7th day, which was 1.4 times higher than 61.9% in the drained paddy. Taken together, the findings suggest that direct contact of the insect's body with sufficient amounts of spray solution and the maintenance of paddy irrigation can enhance the controlling effect of EFOMs. These findings will be valuable in developing an optimal S. lurida control strategy for application in rice paddy fields in the near future.

Changes of Dry Matter Productivity and Feed Value of Forage Barley and Italian Ryegrass According to Cultivation Conditions in Mid-west Plain of Korea (중서부 평야지에서 재배조건에 따른 청보리와 이탈리안 라이그라스의 건물생산성 및 사료가치 변화)

  • Seo, Jong Ho;Kwon, Young Up;Cho, Ga Ok;Han, Ouk Kyu;Gu, Ja Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.2
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    • pp.84-90
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    • 2018
  • Cultivation of high-quality forage crop by using fallow field during winter is required for national high feed self-sufficiency and establishment of self-supply system of high-quality forage crop. Field experiments for cultivation of high-quality winter forage crop were conducted at the paddy and upland fields in Cheonan and Anseong city with treatments of single Italian ryegrass(IRG) and IRG mixed with forage barley at the paddy field and of single forage barley and forage barley mixed with IRG at the upland field in the Mid-west plain. Several cultivation conditions such as broadcasting IRG seed under standing rice, sowing time, tillage method, drainage condition, mixed sowing with forage barley were compared to know the change of growth, yield and quality of winter forage crop. In particular, over-wintering rate and dry matter yield were decreased significantly in late-sown IRG and moisture-stressed forage barley. Yield and quality of forage crops were increased by sowing after tillage, mixed sowing of IRG with barley at the paddy field with good drainage. High yield as much as dry matter $10MT\;ha^{-1}$ with high feed value could be obtained by early sowing of feed barley mixed with IRG at the upland field. Cultivation conditions such as early sowing, sowing after tillage, drainage management are required for higher dry matter yield, quality and stable cultivation of winter forage crops in the mid-west plain of Korea.

Screening of 94 Plant Species Showing ACE Inhibitory Activity (식물자원으로부터 Angiotensin Converting Enzyme 저해활성 탐색)

  • Yun, Jeong-Sik;Chung, Byung-Hee;Kim, Na-Young;Seong, Nak-Sul;Lee, Hyeon-Yong;Lee, Jin-Ha;Kim, Jong-Dai
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.3
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    • pp.246-251
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    • 2003
  • Angiotensin converting enzyme(ACE) belongs to the class of zinc protease and plays an important role in the regulation of blood pressure. In this experiment, we investigated the inhibitory activities of ninety four plant extracts on ACE. The extracts were prepared by water and refluxing with 70% and 100% methanol. Among the extracts, two plant extracts such as Cassia tora, Persicaria cochinchinensis Kitagawa showed more than 60% inhibitory activities, and Foeniculum vulgare Gaertner, Scutellaria baicalensis Georgl, Caragana sinica (Buchoz) Rehder, Inula britannica var. chinensis showed $45.2{\sim}49.7%$ inhibitory activities. Twenty eight plant extracts such as Hemerocallis fulva L, Camptotheca acuminata Decne, Inula britannica var. chinensis, Xanthium strumarium, Polygonatum odoratum, Phellodendron amurense Rupr, Coix lachryma-jobi var. mayuen, Prunus ansu, Hibiscus mutabilis L, Thchosanthes kirilowii, Helianthus annuus, Juglans sinensis showed $30.3{\sim}39.7%$ Inhibitory activities. These results suggest that plant extracts which contain high ACE inhibitory activities may be useful as anti-hypertension agents and to the treatment of hypertension.

Medicinal Herbal Complex Extract with Potential for Hair Growth-Promoting Activity (발모효과를 가지는 한방복합처방단)

  • Lee, Jun Young;Im, Kyung Ran;Jung, Taek Kyu;Lee, Myoung-Hee;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.38 no.4
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    • pp.277-287
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    • 2012
  • To develop new therapeutic materials to prevent hair loss and enhance hair growth, we developed a medicinal herbal complex extract (MHCE) using 23 herbs traditionally used in oriental medicine. Medicinal Herbal complex extract was consist of Angelica gigas Nakai, Psoralea corylifolia Linne, Biota orientalis Endlicher, and Eclipta prostrata Linne, Rehmannia glutinosa Liboschitz var. purpurea Makino, Ligustrum lucidum Aiton, Polygonum multiflorum Thunberg, and Sesamum indicum Linne, Sophora angustifolia Sieboldet Zuccarini, Angelica dahurica Benthamet Hooker, and Leonurus sibiricus Linne, Salvia miltiorrhiza Bunge, Prunus persica Batsch, Commiphora molmol Engler, Chrysanthemum indicum Linne, Boswellia carterii Birdwood, Panax ginseng C. A. Meyer, Cnidium officinale Makino, Albizia julibrissin Durazzini, and Corydalis ternata Nakai that have traditionally been used for treating hair loss, preventing gray hair, anti-inflammation, and blood circulation in oriental medicine. In addition, we examined the hair growth effect of MHCE in vitro and in vivo. In vitro, we evaluated the effects of MHCE on cultured HFDPC, HaCaT cells, and murine embryonal fibroblasts (NIH3T3 cells). Also, we evaluated the ability of MHCE to prevent gray hair on murine melanoma cells (B16F1 cells). The hair growth-promoting effect of MHCE in vitro was also observed in vivo using C57BL/6 mice. Our results showed that MHCE significantly increased the proliferation of HFDPC (175 % proliferation at $50{\mu}g/mL$), HaCaT cells (133 % proliferation at $20{\mu}g/mL$), and NIH3T3 cells (120 % proliferation at $50{\mu}g/mL$). MHCE also showed consistent melanogenesis in B16F1 cells (154 % melanin synthesis at $50{\mu}g/mL$). Moreover, MHCE showed potential for hair growth stimulation in C57BL/6 mice experiments (98 % hair growth area on 4 weeks). These results indicate that MHCE may be a good candidate for promotion of hair growth.

Extracts of Korean Medicinal Plant Extracts Alter Lipogenesis of Pig Adipose Tissue and Differentiation of Pig Preadipocytes In vitro (한국 약용식물 추출물이 In vitro 돼지 지방조직의 지방합성과 지방전구세포의 분화에 영향을 미친다)

  • Choi, Young-Suk;Choi, Kang-Duk;Kim, Sung-Do;Phillip, Owens;Chung, Chung-Soo
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.383-388
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    • 2010
  • Identification of natural compounds that can prevent the development of obesity in vivo is time consuming and expensive. We have used in vitro systems derived from pig adipose tissue to screen simple aqueous or ethanolic extracts of Korean medicinal herbs (KMH) for their anti-adipogenic potential. A total of 183 extracts were tested for their actions in lipogenesis of pig adipose tissue and differentiation of pig preadipocytes. Ethanol extracts were prepared from 72 and aqueous extracts were prepared from 111 medicinal herbs. Both an ethanolic and an aqueous extract were prepared from 65 of these. Thirteen extracts substantially altered rates of lipogenesis in vitro. The effects of KMH on lipogenesis of pig adipose tissue are as follows. Elevens reduced lipogenesis to rates that were more than 40% lower than control and four of these reduced rates of lipogenesis by more than 70%. The most potent anti-lipogenic extracts were those obtained in ethanol from Iridaceae and from Sophora flavescens AIT as well as both the aqueous and ethanolic extracts from Lysimachia vulgaris L. Two extracts, those prepared in water from Caesalpiniae lignum and from Phellodendri cortex, were found to promote rates of lipogenesis in vitro. The effects of KMH on differentiation of pig preadoipocytes are as follows. Twentyeight extracts altered the rates of differentiation of cultured porcine preadipocytes. Sixteen increased and twelve reduced the rates of differentiation of preadipocytes. Extracts prepared in ethanol from Moutan radicis cortex and from Ostericum koreanum and those prepared in water from Angelicae gigantis radix, from Inula henenium L and from Magnolia flos doubled the rate of differentiation of cultured porcine preadipocytes. Ten extracts reduced the in vitro rate of differentiation of porcine preadipocytes by more than 35%. These were the ethanolic extracts from Glycyrrizae radix, Nepetae spica and from Polygala myrtifolia and the aqueous extracts from Amaranthaceae, Asparagus cochinchinesis, Atractylodis rhizoma alba, Citrus junos TANAKA, Cyperus rotundus, Epimedium grandiflorum and from Moutan radicis cortex. Only the ethanolic extract from Polygala myrtifolia was able to both reduce lipogenesis in adipose tissue slices and retard differentiation of cultured preadipocytes. The results of our study will provide meaningful information to identify medicinal herbs which would reduce fat deposition in livestocks and humans.

Screening for Effective Organic Farming Materials for the Control of Cucumber Scab Caused by Cladosporium cucumerinum (오이 검은별무늬병 방제에 효과적인 유기농업자재 선발)

  • Park, So-Hyang;Hong, Sung-Jun;Shim, Chang-Ki;Kim, Min-Jeong;Park, Jong-Ho;Han, Eun-Jung;Kim, Yong-Ki
    • Research in Plant Disease
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    • v.23 no.2
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    • pp.159-167
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    • 2017
  • This study was conducted to evaluate the control efficacy of the organic farming materials on cucumber scab caused by Cladosporium cucumerinum PT1 (KACC 48094). The antifungal activities in vitro as well as the suppressive effect of 43 organic farming materials on the spore germination and germ tube growth by inoculating spore suspension on cucumber seedlings in vivo were investigated. Thirteen organic farming materials inhibited the mycelial growth of C. cucumerinum and nine of these were microbial agents. In the screening using cucumber seedlings, six organic farming materials were very effective with control efficacy value of 90%. Among them, Bacillus amyloliquefaciens M27 provided suppressive effect on both mycelial growth and spore germination against cucumber scab. Finally, nine organic farming materials were selected to test the protective and curative effects, and all chosen organic farming materials significantly suppressed disease incidence when applied in the preventive action, in comparison with the curative action. Especially, Bordeaux mixture I and III gave excellent protective control efficacy with control values of 96.7% and 73.3%, respectively, whereas its curative control effect was significant low. Among these, only Thymus quinquecostatus+Sophora extract showed curative activity, although the control value was as low as 50%. This study suggests that cucumber scab can be controlled by some organic farming materials in the farmhouses under comparatively cold and wet condition and protective treatment is more important and efficient.

Eco-Friendly Organic Pesticides (EFOP)-Mediated Management of Persimmon Pests, Stathmopoda masinissa and Riptortus pedestris (식물 및 미생물 유래 유기농자재 살충효과: 단감해충 감꼭지나방, 톱다리개미허리노린재)

  • Kim, Jong Cheol;Yu, Jeong Seon;Song, Min Ho;Lee, Mi Rong;Kim, Sihyeon;Lee, Se Jin;Kim, Jae Su
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.319-327
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    • 2016
  • Chemical pesticides have been used to control persimmon pests, however the overuse of the pesticides caused insect resistance, followed by failure in pest management and residual problems. Herein we investigate the potential of eco-friendly organic pesticides (EFOP) on the control persimmon pests, Stathmopoda masinissa (persimmon fruit moth) and Riptortus pedestris (bean bug). Ten commercially available plant-derived organic pesticides and one microbial pesticide were sprayed on the target insects in laboratory conditions. The chemical pesticide, buprofezin+dinotefuran wettable powder served as a positive control. In the first bioassay against persimmon fruit moth, alternatively Plutella xylostella larvae were used due to the lack of persimmon fruit moth population from fields, and three organic pesticides showed high control efficacy, such as pyroligneous liquor (EFOP-1), the mixture of Chinese scholar tree extract, goosefoot and subtripinnata extracts (EFOP-2) and Bacillus thuringiensis subsp. aizawai NT0423 (EFOP-11). When the three selected organic pesticides were treated on the persimmon fruit moths, the EFOP-2 treatment showed the highest control efficacy: 27.7% (5 days), 13.3% (7 days) and 6.7% (10 days) of survival rates. In the bioassay against bean bugs, the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2 and EFOP-9) and the extracts of sophora and derris (EFOP-10) showed high control efficacy, particularly the highest in the treatment of EFOP-2: 20.0% (5 days) and 16.7% (10 days) of survival rates. These results suggest that the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2) has high and multiple potential in the management of the persimmon pests.