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Effects of Gamma-Irradiation on the Sterilization of Primordial Germ Cells in Quail (메추리 원시생식세포 감소를 위한 감마선 조사의 효과)

  • Park, Kyung-Je;Kim, Tae-Min;Lee, Hyung-Chul;Jang, Hyun-Jun;Song, Gwon-Hwa;Han, Jae-Yong
    • Korean Journal of Poultry Science
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    • v.37 no.2
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    • pp.139-143
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    • 2010
  • Quail is a very useful animal model for studying vertebrate development because of its small body size and unique reproductive traits. This species is also ideal model for producing germline chimeras via transferring exogenous primordial germ cells (PGCs) into the recipient embryo. To increase the contribution efficiency of donor PGCs into recipients' tissues, decreasing the population of endogenous PGCs has been rate-limiting factor. We therefore conducted this study to investigate if gamma ($\gamma$)-irradiation depletes endogenous PGCs in developing quail embryo. Firstly, freshly laid stage X quail embryos were irradiated with various output of $\gamma$-irradiation and its teratogenic effect on the embryo was evaluated. Although a dose-dependent increase in the number of embryo showing malformation was found as the output increased (0, 250, 500, 750, and 1,000 rads), only a maximum of 10.1% of embryos were abnormal in 1,000 rads. Immunocytochemical analysis using the QCR1 antibody, which is specific marker for quail PGCs, was conducted to analyze the effect of sterilization. As results, $\gamma$-rays at a dose-rate of 500 rads/73 sec onto undeveloped stage X embryo significantly reduced the number of germ cells to an average of 75.55 % and 82.03 % in male and female embryos, respectively. We conclude that $\gamma$-ray selectively targets PGCs while affects minimally to the somatic development in quail embryo. Our results will not only provide important data for germline chimera production but can be used for analyzing the effect of ionized rays on the differentiating germ cells in various stages during animal development.

Prevalence of Antibodies to Hepatitis A in the Healthy Children in Inchon-Kyunggi Prefecture (경인 지역 소아의 A형 간염 항체 보유율)

  • Kwon, Young Se;Hong, Young Jin;Choe, Yon Ho;Kim, Soon Ki;Son, Byong Kwan;Kang, Moon Su;Pai, Soo Hwan;Hong, Kwang Sun
    • Pediatric Infection and Vaccine
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    • v.5 no.2
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    • pp.239-244
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    • 1998
  • Purpose : The incidence of hepatitis A virus infection in Korea has decreased recently. However, the prevalence in children and young adults is on an increasing trend in Inchon-Kyunggi prefecture. Economic development that leads to better living conditions and improves hygiene facilities has reduced the rate of HAV infection. This study was designed to evaluate the recent seroprevalence rate of hepatitis A in children and adolescents. Methods : Study population consisted of 612 children and adolescents aged 1 to 17 years in Inchon-Kyunggi prefecture. Serum samples were collected between 1996 and 1997 and tested for anti HAV by Microparticle Enzyme Immunoassay(MEIA). Results : According to age, the seropositive rates were 10.5%(male 13%, female 6.7%) in 1 year-old group, 0% in 3, 5, 11 and 13 year-old group, 6%(male 8%, female 4%) in 15 year-old group, and 11%(male 14%, female 8%) in 17 year-old group. Conclusion : This study showed that seropositive rate of HAV infection increased with age. While it revealed extremely low incidence of HAV infection in children less than 13 years old, they are also likely 10 be exposed 10 the infection. Our findings suggest that the effective administration of vaccination is needed in selected and high risk groups.

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A Study on the Sociomedical Status of Prostitutes in Busan Area (부산일부지역 특수업태부들의 사회의학적인 조사)

  • Choi, Jin-Hae
    • Journal of Preventive Medicine and Public Health
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    • v.10 no.1
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    • pp.125-133
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    • 1977
  • The author have performed, from June to July 1976, a sociomedical study on the 314 prostitutes which was engaged in tourism in Dongrae, Busan. The obtained results were as follows: 1. Age distribution was from 20 years old to 34 years old and the highest as 36.6% was 23-25 years old group. Most of the respondents as 95.2% were under 31 years old group. 2. Distribution of respondents by educational level showed that the group below the graduated primary school was 47.2%, middle school 29.3% and high school 57.0% in that order. 5. By the occupation before be ruined, none was 34.5%, waitress at saloon 24.2%, hostess 14.3%. 4. By distribution of birth place, most of the respondents as 64.0% were Gyeongsang Do(37.9%) and Busan City (26.1%). 5. Distribution of respondents by family status showed that only mother was highest as 43.9% and 82.2% of respondents have been supported their family. 6. Monthly incomes of respondents were distributed from 50,000 Won to 250,000 Won, and 50,000-100,000 Won group was the highest as 46.2%. In monthly sundry expenses, 20,000-30,000 Won group was the highest as 27.1%. 7. Monthly supporting expenses to their family were distributed from 10,000 Won to 50,000 Won and the highest as 26.7% was 10,000-20,000 Won group. Savings per month were distributed from 10,000 Won to 60,000 Won and 50,000-60,000 Won group was the highest as 22.3%. 8. Distribution of respondents by status of luxury showed that experiences of drinking was 20.4% and smoking was 55.7%. 9. By motive of falling into the ruin, economical causes was 41.7%. Most of the respondents as 95.0% expected under 3 years in prearranged period of their occupation. 10. By number of monthly entertainment, 5-10 persons as 58.0% was the highest. 11, Distribution of respondents by status of venereal disease and pulmonary tuberculosis showed that positive of gonorrhea was 8.0%, syphilis 1.6%, and pulmonary tuberculosis was 1.6%.

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Development of a Theme-Selection Activity in 'Clothing Life' in Relation to SDGs for the Free Semester Program (지속가능발전목표(SDGs) 성취를 위한 의생활 자유학기제 주제선택활동 프로그램 개발)

  • Choi, Ye Ji;Park, Mi-Jeong;Shim, Huen-Sup
    • Journal of Korean Home Economics Education Association
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    • v.32 no.3
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    • pp.27-48
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    • 2020
  • The purpose of this study is to develop a theme-selection activity in 'clothing life' in relation to SDGs(Sustainable Development Goals) for the free semester program. After analyzing the contents of the 'clothing life' area of middle school home economics textbooks based on the SDGs, the content system and learning goals were set. Then a program was developed and the validity and the feasibility of the program were verified. As a result of the analysis of textbooks, the contents of 'clothing life' included all the three basic dimensions of social development, economic growth, and environment protection, yet focus only on 1 or 2 goals of each area. Based on the results of the analysis, a 'Righteous(義) Clothing(衣) Life' program was developed. The developed program consists of teaching-learning process plans and teaching-learning materials in eight class periods, including 'The future everyone dreams of' based on SDG12, 'Two faces of fast fashion' based on SDG1, SDG5, SDG8, SDG10, 'Living as Homoclimatus' based on SDG13, and 'The future we create' based on SDG9 and SDG12. Through the expert evaluation process for the developed program, the program's teaching and learning adequacy and feasibility were reviewed and feedback was actively reflected to correct and supplement the program. Through this study, it is expected that it will contribute to laying the foundation for establishing home economics as a subject that educates citizens who practice sustainable life, and a pivotal subject in education for sustainable development.

A STUDY OF PARENTAL KNOWLEDGE AND ATTITUDE ABOUT INFANT ORAL HEALTH CARE (유아의 구강관리에 관한 보호자의 인지도 조사)

  • Kim, Myoung-Jin;Shun, Ye-Kyung;Shim, Youn-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.2
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    • pp.292-299
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    • 2000
  • The purpose of this study was to investigate parental knowledge and attitude and practices relating to infant oral health care and to promote their motivations to their children's oral health care. Authors interviewed 350 persons in Ilwon-Dong and whose children were between 0 to 36 months old and acquired questionnaire from them. The questionnaire constituted of questions about general knowledge of tooth eruption, methods of oral hygiene care, and opinions about preventive dental visit. The collected data were analysed and compared with other researches. The important results obtained from this study were : 1. Females and highly educated persons had more dental knowledge than males and poorly educated persons. But age and economic status did not affect their level of dental knowledge. 2. Fifty nine percents of respondents thought that tooth began to be formed before birth. 3. Seventy six percents of respondents thought that infant oral health care should begin before tooth eruption, but actually only 63% started oral hygiene care before age one. 4. Seventy percents of respondents used gauze for infant oral care. 5. Fifty seven percents of respondents still gave bottles to their children after they reached age one or more and 55% of respondents never heard of nursing caries. 6. Ninety percents of respondents agreed that carious deciduous teeth need restorations, and 31% of respondents thought that age two is the appropriate time for the first dental visit. 7. Forty seven percents of parents did not think that dental caries is an infectious disease. 8. Only Thirty three percents of parents visited dental clinics for examination and prevention during pregnancy and only 11% of expecting parents were offered dental health care education in pediatric or obstetrician clincs. 9. Information about oral health care was mostly given from baby megazines. 10. Overall parental knowledge about infant oral health care was not sufficient to maintain appropriate dental care for infants.

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A STUDY ON THE INFECTION LEVEL OF S. MUTANS AND LACTOBACILLUS AND ITS RELATIVITY WITH CARIES RISK FACTORS IN 18 MONTH-OLD INFANTS (18개월 유아의 S. mutans와 Lactobacillus감염수준과 우식위험요인의 상관성에 관한 연구)

  • Ahn, Jin-Gong;Chung, Jin;Jeong, Tae-Sung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.3
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    • pp.388-400
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    • 2006
  • The time 18 months after birth is very important for the prevention of early childhood caries because this time is proper to assure the completion of weaning and to perform the diet counseling, and caries process is initiated or actively aggravated under the total influence of feeding pattern from this period. For the purpose of examining the infection status of Streptococcus mutans and Lactobacillus of 18 month old infants and their mothers, and analyzing the correlation between infection status versus various caries-risk factors, this study was performed on the 60 infants of 18 months old and their mothers through the microbiological screening of saliva samples and direct questionnaire to mothers about the caries risk factors. And the results were as follows, 1. The higher the mother's infection level of S. muntans, the higher the infant's infection level and transmission rate. 2. Compared with non-caries group, higher infection level of S. mutans in both infants and mothers and transmission rate were revealed in caries group. 3. The higher the infant's infection level of S. mutans, the higher the caries prevalence and deft values, the less the monthly income of the parents, the longer the period of breast feeding, and the higher the consumption rate of sugared snacks. 4. Most of Lactobacillus infected infants were revealed to be infected also with S. mutans and to have caries. 5. The higher deft value, less parents' monthly income, more frequent daily nursing and more irregular snacking patterns were found among Lactobacillus infected group compared with not-infected group.

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Quality of Yellow Poplar (Liriodendron tulipifera) Seedlings by the Method of Seedling Production (백합나무 양묘방법에 따른 묘목품질 비교)

  • Ryu, Keun-Ok;Song, Jeong-Ho;Choi, Hyung-Soon;Kwon, Hae-Yun;Kwon, Yong-Rak
    • Journal of Korean Society of Forest Science
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    • v.96 no.3
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    • pp.307-316
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    • 2007
  • Yellow poplar (Liriodendron tulipifera L.) has low germination rate relatively other species, so the seedling production of Yellow poplar is a hard task. Accordingly this study was conducted to determine the optimal germination conditions for healthy seedling production and to promote survival rate after afforestation. Gemination percentage was examined at different media and seed covering materials using planting flats in the greenhouse. The best germination percentage was observed in sand for media and compound soil for covering materials. But it was time to transplant, seedlings became a poor character (i.e. height, root length, number of root, dry weight) in sand for media. In order to produce healthy seedlings, each different medium was compounded with TKS-2 (this is a gardening bed soil.) in the ratio 1:1 (v/v.), and compared two conditions. Quality of seedling was better than not mixed TKS-2 into each medium. Transplanting seedlings from greenhouse to nursery grew up rapidly 2 months later (early in August~early in October). Growth amount during two months corresponded to 85.6% and 71.3% in total growth amount of height and diameter at root collar, respectively. In the case of the competition-density effect on yellow-poplar seedlings, direct seedling produced the maximum 35 standard seedlings above 8 mm of root collar diameter per $m^2$, while transplanting seedling produced the maximum 64 standard seedlings per $m^2$. And produced seedlings of two way were significantly different rootlet while axial root and lateral root was not significantly different.

Korea and Japan Comparison Study of Distribution Industry: Focus on Input-out Analysis (유통산업의 한일비교 연구 - 산업연관분석을 중심으로 -)

  • Jho, Kwang-Hyun
    • Journal of Distribution Research
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    • v.16 no.5
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    • pp.171-192
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    • 2011
  • This paper focuses on the retail industry of industrial share of the GDP, productivity of distribution industry and input-out analysis between Korea and Japan, also results are summarized as follows. First, the share of GDP in agriculture, forestry and fisheries of the both countries is falling. That of manufacture increases in South Korea, while Japan is falling. While distribution industry shows vice versa. Employed population by industry is falling both countries also. The relative labor productivity shows that agriculture, forestry and fisheries, retail industry needs more labor, while manufacture has been met for both countries. Second, compare to Japan, the retail industry of Korea has been increased since 1990. Likewise, overall productivity of distribution industry in Korea has been increased while almost that of Japan has declined. Third, production inducement effects of Japan are greater than that of Korea. On the other hand, import inducement effects show vice versa. Fourth, as shown from the final demand of distribution industry and the rate of dependence on production inducement, we can see that the “increase in stocks” increases while gross government fixed capital formation shows vice versa. Korea's private consumption expenditure increases while Japan shows versa. South Korea's government consumption expenditure and exports are rising, on the other hand, that of Japan is declining. Fifth, the rate of dependence on distribution industry and import inducement shows the same tendency from both countries. As we can see from the private consumption expenditure, government consumption expenditure, gross government fixed capital formation, gross private fixed capital formation, increase in stocks, the rate of dependence on import inducement is more effective than the rate of dependence on production inducement. While the exports are comparatively ineffective. Sixth, the degrees of influence of retail industry are similar between Korea and Japan, while sensitivity of the Korean industry has been weakened. In this sense, strong policies are needed to boost the industry. Seventh, the investments in the retail industry of Korea showed the public-led trend, while Japan showed private sector-led investment trend. The investment trend of Korea's retail industry will be switched into private sector-led investment step by step in the future. This finding will be an important clue to set the policy direction of Korea distribution industry. Finally, both Korea and Japan are still in need of employment in retail industry. Not addressed in this paper, such as value-added-induced effects, employment inducement effect, will be remaining challenges in the following paper.

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A Study on the Collection and Marketing Structure of Sap Water of Acer mono (고로쇠나무 수액(樹液)의 채취(採取)와 유통구조(流通構造)에 관(關)한 연구(硏究))

  • An, Jong Man;Kang, Hag Mo;Kim, Jun Sun
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.391-403
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    • 1998
  • The study was carried out to devise a proper measure to increase the income of mountain villagers by producing sap water of Acer mono, and to make the most of sap water as local specialty to contribute to the local economy of mountain villages. All the processes from collecting to marketing of sap water of Acer mono was investigated. The survey was done from mid-January to mid-February in the 3 major sap water collecting regions, Toji-myon Kurey-gun(Piagol area of Mt. Chiri), Okryong-myon Kwangyang city(Mt. Baekun), and Jookhack-ri Sunchon(Mt. Chokey). A total of 90 householders who collect sap water, to say again, 30 householders in each region, were interviewed personally to make up questionnaires. The habitual or general practices about collecting sap water, the selling price, the sales process, labor power to collect and carry down, carrying distance and facilities, sales income and side income, and family income were investigated and examined. Spots of collecting sap water were not concentrated but scattered all over the collecting area. Collecting method, collecting amount, sales process, and selling price varied with the village and region. Sap water was collected by tapping or boring method, the latter of which was widely used in lots of regions except in Sunchon. Although the amount of sap production per family varied with region, the average amount was about 1,350 liters. Of all the sap water collected, 44% was consumed by drinking of on-the-spot visitors and 36% was sold by order, etc. Sap water was sold at the price varying from 10,000 won to 60,000 won per 18 liters. The average selling price was 41,000 won, but selling prices of 43,000 won and 45,000 wan amounted to 38% and 25%, respectively.

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Framework of Stock Market Platform for Fine Wine Investment Using Consortium Blockchain (공유경제 체제로서 컨소시엄 블록체인을 활용한 와인투자 주식플랫폼 프레임워크)

  • Chung, Yunkyeong;Ha, Yeyoung;Lee, Hyein;Yang, Hee-Dong
    • Knowledge Management Research
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    • v.21 no.3
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    • pp.45-65
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    • 2020
  • It is desirable to invest in wine that increases its value, but wine investment itself is unfamiliar in Korea. Also, the process itself is unreasonable, and information is often forged, because pricing in the wine market is done by a small number of people. With the right solution, however, the wine market can be a desirable investment destination in that the longer one invests, the higher one can expect. Also, it is expected that the domestic wine consumption market will expand through the steady increase in domestic wine imports. This study presents the consortium block chain framework for revitalizing the wine market and enhancing transparency as the "right solution" of the nation's wine investment market. Blockchain governance can compensate for the shortcomings of the wine market because it guarantees desirable decision-making rights and accountability. Because the data stored in the block chain can be checked by consumers, it reduces the likelihood of counterfeit wine appearing and complements the process of unreasonably priced. In addition, digitization of assets resolves low cash liquidity and saves money and time throughout the supply chain through smart contracts, lowering entry barriers to wine investment. In particular, if the governance of the block chain is composed of 'chateau-distributor-investor' through consortium blockchains, it can create a desirable wine market. The production process is stored in the block chain to secure production costs, set a reasonable launch price, and efficiently operate the distribution system by storing the distribution process in the block chain, and forecast the amount of orders for futures trading. Finally, investors make rational decisions by viewing all of these data. The study presented a new perspective on alternative investment in that ownership can be treated like a share. We also look forward to the simplification of food import procedures and the formation of trust within the wine industry by presenting a framework for wine-owned sales. In future studies, we would like to expand the framework to study the areas to be applied.