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http://dx.doi.org/10.5536/KJPS.2010.37.2.139

Effects of Gamma-Irradiation on the Sterilization of Primordial Germ Cells in Quail  

Park, Kyung-Je (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Kim, Tae-Min (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Lee, Hyung-Chul (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Jang, Hyun-Jun (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Song, Gwon-Hwa (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Han, Jae-Yong (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Publication Information
Korean Journal of Poultry Science / v.37, no.2, 2010 , pp. 139-143 More about this Journal
Abstract
Quail is a very useful animal model for studying vertebrate development because of its small body size and unique reproductive traits. This species is also ideal model for producing germline chimeras via transferring exogenous primordial germ cells (PGCs) into the recipient embryo. To increase the contribution efficiency of donor PGCs into recipients' tissues, decreasing the population of endogenous PGCs has been rate-limiting factor. We therefore conducted this study to investigate if gamma ($\gamma$)-irradiation depletes endogenous PGCs in developing quail embryo. Firstly, freshly laid stage X quail embryos were irradiated with various output of $\gamma$-irradiation and its teratogenic effect on the embryo was evaluated. Although a dose-dependent increase in the number of embryo showing malformation was found as the output increased (0, 250, 500, 750, and 1,000 rads), only a maximum of 10.1% of embryos were abnormal in 1,000 rads. Immunocytochemical analysis using the QCR1 antibody, which is specific marker for quail PGCs, was conducted to analyze the effect of sterilization. As results, $\gamma$-rays at a dose-rate of 500 rads/73 sec onto undeveloped stage X embryo significantly reduced the number of germ cells to an average of 75.55 % and 82.03 % in male and female embryos, respectively. We conclude that $\gamma$-ray selectively targets PGCs while affects minimally to the somatic development in quail embryo. Our results will not only provide important data for germline chimera production but can be used for analyzing the effect of ionized rays on the differentiating germ cells in various stages during animal development.
Keywords
chimeras; primordial germ cells (PGCs); gamma ($\gamma$)-irradiation; stage X quail embryos;
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