• Journal of agriculture & life science
• /
• v.46 no.1
• /
• pp.163-176
• /
• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.

• The Journal of Engineering Geology
• /
• v.32 no.1
• /
• pp.173-191
• /
• 2022

In this study, the shear wave velocity of the ground was measured using Flat TDR, and the precision analysis of the measured value and the verification of field applicability were performed. The shear wave velocity measurement value was derived in the field using the piezo-stack combined in the Flat TDR. analyzed. As a result of the experiment, the average value of the change in shear wave speed at the time of grout material injection was 10.15 m/s at the beginning of age, and the average value of the change in shear wave speed after the 7th to 14th days was 65.99 m/s, showing a tendency to increase with age. Also, it was found that dry density and shear wave speed increased as the water content increased on the dry side, and that the dry density and shear wave rate decreased as the water content increased on the wet side as the water content increased. The shear modulus value derived from the field test was confirmed to be a minimum of 17.36 MPa and a maximum of 28.13 MPa, confirming a measurement value similar to the reference value. Through this, it can be seen that the measured value of the shear modulus using Flat TDR is reliable data, and it can be determined that the compaction management of the site can be effectively managed in the future.

• Composites Research
• /
• v.30 no.5
• /
• pp.316-322
• /
• 2017

Carbon fiber is a material with excellent mechanical, electrical and thermal properties, which is widely used as a composite material made of a polymer matrix. However, this composite material has a weak point of interlaminar delamination due to weak interfacial bond with polymer matrix compared with high strength and elasticity of carbon fiber. In order to solve this problem, it is essential to use reinforcements. Due to excellent mechanical properties, graphene have been expected to have large improvement in physical properties as a reinforcing material. However, the aggregation of graphene and the weak interfacial bonding have resulted in failure to properly implement reinforcement effect. In order to solve this problems, dispersibility will be improved. In this study, functionalization of graphene nanoplatelet was proceeded with melamine and mixed with epoxy polymer matrix. The carbon fiber reinforced polymer composites were fabricated using the prepared graphene nanoplatelet/epoxy and flexural properties and interlaminar shear strength were measured. As a result, it was confirmed that the dispersibility of graphene nanoplatelet was improved and the mechanical properties of the composite material were increased.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.33 no.11
• /
• pp.88-94
• /
• 2005

This paper is a study on the blade-type optical bench satisfying stiffness and thermal pointing error requirements for earth observation satellite. According to shape requirements, optical bench is designed. Because it does not satisfy the stiffness requirement, the stiffener is added on the outer/inner area of optical bench. But it does not meet the thermal pointing error requirement. So symmetrical structure is suggested with platform support structure attached on the upper/lower part of platform. Although it has better value than previous case, it still does not meet the thermal pointing error requirement. Based on the results of prior cases, optical bench finally designed, which satisfied both the stiffness and thermal pointing error requirements. Next conclusions follow from this design. It is efficient to increase thickness of platform facesheet, add stiffener and increase blade number to raise stiffness. It is effective to connect component consisting of same material and design optical bench having symmetrical structure to lower thermal pointing error.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.3 no.1
• /
• pp.1-6
• /
• 2015

Recently, lots of researches on concrete with high volume mineral admixture such as ground granulated blast furnace slag (GGBFS) have been carried out to reduce $CO_2$. It is known that the precast concrete has an advantage of high strength at early age due to steam curing, even if concrete has high replacement level of mineral admixture. However it demands the investigation of compressive strength properties according to steam curing regimens. In this study, concretes with water-binder ratio of 32, 35% and water content of 135, 150, $165kg/m^3$ were produced to investigate compressive strength properties of high volume (60% by mass) GGBFS cement concrete according to steam curing regimens. Then steam curing was implemented with the maximum temperature of 50, $60^{\circ}C$ and steaming time of 5, 6, 7 hours. From the test results, it was found that steam curing was effective to raise early strength of high volume GGBFS cement concrete, but 28 day compressive strengths of steam cured specimens were lower than those of water cured specimens. Thus, a further study would be needed for the optimum steam curing regimens to satisfy target demolded strength and specified strength for the application of high volume GGBFS cement concrete to precast concrete members.

• Journal of Korean Medical classics
• /
• v.19 no.2 s.33
• /
• pp.46-52
• /
• 2006

${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 내함한의학이론(內含韓醫學理論) 기중삼응삼양적내용비상난(其中三陰三陽的內容非常難) 기원인사(其原因是)${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 중삼음삼양적내용비상복집(中三陰三陽的內容非常複雜), 특별시삼음삼양적시간배속매편화일양(特別是三陰三陽的時間配屬每篇不日樣) 본론문통과관우(本論文通過關于子) ${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 중삼음삼양적시간배속진행연구(中三陰三陽的時間配屬進行硏究), 득출여하결론(得出如下結論), 운기지주기순서춰시오행(목화토금수)순서(運氣之主氣順序就是五行(木火土金水)順序), 저설명주기변화주요근거형지변화(這說明主氣變化主要根據形之變化), 운기지객기순서취시기지대(運氣之客氣順序就是氣之大小)(일양(一陽)${\longrightarrow}$이양(二陽)${\longrightarrow}$삼양(三陽)${\longrightarrow}$일음(一陰)${\longrightarrow}$이음(二陰)${\longrightarrow}$삼음(三陰))${\longrightarrow}$, 저설명객기변화주요근거기지변화(這說明客氣變化主要根據氣之變化) ${\ulcorner}$소문맥해(素問脈解)${\lrcorner}$ 적삼음삼양월별배속시음관(11월:태음)(的三陰三陽月別配屬是陰關(11月:太陰)${\longrightarrow}$양관(1월:태양)(陽關(1月:太陽)${\longrightarrow}$음합(3월:궐음)(陰闔(3月:厥廠)${\longrightarrow}$양합(5월:양명)(陽闔(5월:陽明))${\longrightarrow}$음추(7월:소음)(陰樞(7月:少陰)${\longrightarrow}$양추(9월:소양)(陽樞(9月少陽). 태양소음시음양세력적기초(太陽少陰是陰陽勢力的基礎), 소이상위표리(所以相爲表裏). 소양소음시음양세력적중간(少陽少陰是陰陽勢力的中間), 소이사위표리(所以杞爲表裏) 태음궐음시음양세력적전성기(太陰歐陰是陰陽勢力的全盛期), 소이상위표리(所以相爲表裏) 차료리적의사반영경락류주(此表養的意思反映經絡流注). 태음양명경류주신체전면(太陰陽明經流注身體前面), 태양소음경류주신체후면( 太陽少陰經流注身體後面 ), 소양궐음경류주신체측면(少陽厥陰經流注身體側面). ${\ulcorner}$령추경맥(靈樞經脈)${\lrcorner}$적12경맥류주순서시태음양면경(的12經脈流注順序是太陰陽明經)${\longrightarrow}$소음태양경(少陰太陽經)${\longrightarrow}$궐음소양경적표리경락위주(厥陰少陽經的表裏經絡爲主). 수선매기류주태음양명경적이유(首先脈氣流主太陰陽明經的理由), 취시후천수곡정미화호흡대기결합이형성경맥지기(就是後天水穀精微和呼吸大氣結合而形成經服之氣). 지후맥기류주소음태양경(之後脈氣流注少陰太陽經), 설명순행태음양명경적후천영양물질전입한열경락이위신(說明盾行太陰陽陽明經的後天營養物質轉入寒熱經絡而爲寫薪), 기차맥기순해궐음소양경적(其次脈氣盾行厥陰少陽經的), 인위한열경락지화기피궐음소양경지풍기조절이후조절조습경락(因爲寒熱經絡之火氣被厥陰少陽經之風氣調節而後調節操濕經絡), 비여작반시(比如作飯時), 화기취시소음태양경(火氣就是少陰太陽經), 풍기취시궐음소양경(風氣就是厥陰少陽經), 정내지반취시태음양명경.(鼎內之飯就是太陰陽明經). ${\ulcorner}$령추음양계일월(靈樞陰陽緊日月)${\lrcorner}$ 적삼음삼양월별배속주요시추관합합관추순서(的三陰三陽月別配屬主要是樞關闔闔關樞順序). 저양배서적원인시개문화관문과정(這樣排序的原因是開門和關門過程). 재춘하(在春夏), 양경락지맥기여개문관문과정(陽經絡之脈氣如開門關門過程), 수착추관합합관추순서순행(隨看樞關闔闔關樞順序盾行), 재추동(在秋冬), 음경락지맥기여개문폐문과정(陰經絡之脈氣如開門閉門過程), 수착추관합합관추순행(隨看樞關闔闔關樞盾行).

• Journal of Bio-Environment Control
• /
• v.1 no.2
• /
• pp.123-134
• /
• 1992

In order to verify the usability of the greenhouse for aquaculture with nutrient culture synchronously and to obtain the fundamental data fir the establishment of efficient farming technology, the characteristics of microclimate and the growth of leafy vegetables were examined. Tilapia averaged 428.6 g grew to 784 g(1.83 times) for 147 days from May 29 to Oct. 21 and fingerlings averaged 12.9 g grew by 1.37 times for 61 days from Sep. 13 to Nov. 12. The growth of vegetables such as water dropwort, leaf lettuce, Chinese cabbage, and Welsh onion in the greenhouse was better for aquaculture with nutrient culture than for nutrient culture only. Between above two greenhouses, pH and EC of nutrient solution was same but the temperature different by about 2$^{\circ}C$. Average day temperature, relative humidity, and $CO_2$ concentration were higher by 2.9$^{\circ}C$, 6%, and 200 ppm in the greenhouse for aquaculture with nutrient culture, respectively. Net assimilation rate of vegetables in the greenhouse was a little higher for aquaculture with nutrient culture than for nutrient culture only. Therefore, provided aquaculture and nutrient culture are carried out in the same greenhouse, the saving effect of heating cost as well as the additional promotive effects of vegetable and tilapia growth can be obtained.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.49 no.1
• /
• pp.12-19
• /
• 2012

Recently, the market of skin-care device has been steadily growing up. In this paper, we tried to develop a personal compound stimulus device more competitive than existing products. As the compound stimulus, biochemical stimulus of herbal extraction fluid, thermal stimulus of plate-shaped carbon fiber heater, and optical stimulus of near infrared LED were selected. By some evaluation tests, the thermal stimulation part and the optical stimulation part were found to be developed properly. Additionally, the efficacy of the mixed stimulus of thermal and optical stimulation was tested in C2C12 mouse myoblast. Through RT-PCR analysis, it was found that, by the developed compound stimulus, the expression of collagen I mRNA and collagen III mRNA increased by 4.9 and 1.3 times respectively.

• Journal of the Korea Concrete Institute
• /
• v.17 no.4 s.88
• /
• pp.621-628
• /
• 2005

Recently, it is noticed that autogenous shrinkage of high-performance concrete causes early crack in high performance concrete structures. The purpose of the present study is to derive a realistic equation to estimate the autogenous shrinkage of high performance concrete and to apply to structural analysis. For this purpose, several series of concrete specimens have been tested. When water-binder ratio is fixed to $30\%$, major test variables were the type and contents of mineral admixture. The autogenous shrinkage of HPC with fly ash slightly decreased than that of OPC concrete, but the use of blast furnace slag increased with the autogenous shrinkage. A prediction equation to estimate the autogenous shrinkage of HPC with mineral admixture was derived and proposed in this study. The proposed equation show reasonably good correlation with test data on autogenous shrinkage of HPC with mineral admixture. The finite element program developed in this study provides the useful tool for the flexural analysis including the autogenous shrinkage model. By this program, we know that the tensile stress considering the autogenous shrinkage of reinforced concrete structures increase $20\~27\%$ than that not considering.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.23 no.3
• /
• pp.48-66
• /
• 2024

According to the three amended Laws of the Data Economy and the Data Industry Act of Korea, systems for pseudonymous data integration and Data Safe Zones have been operated separately by selected agencies, eventually causing a burden of use in SMEs, startups, and general users because of complicated and ineffective procedures. An over-stringent pseudonymization policy to prevent data breaches has also compromised data quality. Such trials should be improved to ensure the convenience of use and data quality. This paper proposes a prototype system of the Integrated Data Safe Zone based on redesigned and optimized pseudonymization workflows. Conventional workflows of pseudonymization were redesigned by applying the amended guidelines and selectively revising existing guidelines for business process redesign. The proposed prototype has been shown quantitatively to outperform the conventional one: 6-fold increase in time efficiency, 1.28-fold in cost reduction, and 1.3-fold improvement in data quality.