• Title/Summary/Keyword: 견과

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Comparison of methods of DNA extraction from tree nuts (견과류로부터 효율적인 DNA 추출 방법 비교)

  • Suh, Seung-Man;Park, Saet-Byul;Kim, Mi-Ju;Kim, Hae-Yeong
    • Korean Journal of Food Science and Technology
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    • v.50 no.4
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    • pp.357-361
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    • 2018
  • This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.

A Survey of Total Aflatoxins in Food Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Liquid Chromatography Tandem Mass Spectrometry(LC-MS/MS) (HPLC-FLD 및 LC-MS/MS에 의한 식품 중 총아플라톡신 오염실태 조사)

  • Jang, Mi-Ran;Lee, Chang-Hee;Cho, Sung-Hye;Park, Joon-Shik;Kwon, Eun-Young;Lee, Eun-Jin;Kim, So-Hee;Kim, Dai-Byung
    • Korean Journal of Food Science and Technology
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    • v.39 no.5
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    • pp.488-493
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    • 2007
  • A survey for total aflatoxins (aflatoxins $B_1$, $B_2$, $G_1$, and $G_2$) was conducted on 245 cereals and processed cereal products, and 148 nuts and processed nut products in Korea, for a total of 393 commercialized ed samples. The total aflatoxins were quantified by the immunoaffinity column clean-up method with high performance liquid chromatography (HPLC) - fluorescence detection (FLD), and were confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Total aflatoxins(AFs) were detected in 37 samples (9.4% incidence), including 2 millet samples, 1 mixed cereal (sunsik), 1 powdered malt sample, 2 processed cereal products, 6 peanut samples, 22 peanut butter samples, and 1 sample each of almonds, adlay tea, and a processed nut product. The contamination levels were $0.04-2.65{\mu}g/kg$ for aflatoxin $B_1$, and $0.04-5.51{\mu}g/kg$ for total aflatoxins. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected aflatoxins, and all 37 samples showing aflatoxins by HPLC-FLD were confirmed by LC-MS/MS.

Analysis of Total Aflatoxin, Ochratoxin A, Zearalenone, Deoxynivalenol and T-2 Toxin Contamination in Nuts (시중유통 견과류의 총아플라톡신, 오크라톡신 A, 제랄레논, 데옥시니발레놀, T-2 독소의 오염도 조사)

  • Hong, JoonBae;Park, Kun Taek
    • Journal of Food Hygiene and Safety
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    • v.34 no.1
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    • pp.58-64
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    • 2019
  • In the current study, 109 commercial nut samples were collected from different Korean markets and analyzed for the contamination of 5 different mycotoxins (aflatoxin, ochratoxin A, deoxynivalenol, zearalenone, and T-2 toxin) using ELISA kits. The results revealed that the most frequently detected mycotoxin was zearalenone (n=36, 33%), followed by aflatoxin (n=31, 28.4%) and ochratoxin A (n=30, 27.5%). Deoxynivalenol and T-2 toxin were also detected in 22 (20.3%) samples, respectively. Among 109 nut samples, 33 samples (30.3%) were contaminated only with one kind of mycotoxin, whereas 43 samples had at least 2 kinds of mycotoxins. Two samples were contaminated with as many as 4 different mycotoxins, and they were both walnuts. Although the monitoring results revealed the amount of aflatoxin contamination was under the safety criteria, there is no current safety guideline for other kinds of mycotoxins or multiple contaminations in Korea. Therefore, further studies should be performed to reveal the distribution of mycotoxin in different foods and propose appropriate safety guidelines for Korean markets.

Food Classification by the Codex Alimentarius Commission: Cereal Grains, Nuts and Seeds, Herbs and Spices (코덱스의 식품 분류: 곡류, 견과종실류, 허브 및 향신료)

  • Lee, Mi-Gyung
    • Journal of Food Hygiene and Safety
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    • v.34 no.2
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    • pp.212-218
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    • 2019
  • The process of establishing domestic standards on hazardous substances in food safety regulations requires harmonization with standards from the Codex Alimentarius Commission (CAC). For this purpose, food classification by the CAC (Codex Classification of Foods and Animal Feeds) also needs to be clearly understood. Therefore, this paper aimed to introduce the Codex Classification on cereal grains, nuts/seeds and herbs/spices because revisions of the Codex were completed in 2017 for cereal grains and in 2018 for nuts/seeds and herbs/spices. The revised Codex Classification on those foods is briefly summarized as follows. Cereal grains in the domestic food classification by the Ministry of Food and Drug Safety, Korea (MFDS) corresponds to the Codex Group 020 cereal grains with six subgroups. The MFDS's nuts and seeds classification corresponds to three groups in the Codex, namely, Group 022 (tree nuts with no subgroups), Group 023 (oilseeds and oilfruits with 5 subgroups), and Group 024 (seeds for beverages and sweets with no subgroups). The food commodities of herbs and spices are included in two Codex groups, Group 027 (with 3 subgroups) and Group 028 (with 9 subgroups). The number of Codex commodity codes assigned to food commodities was 27 for Group 020, 32 for Group 022, 46 for Group 023, 4 for Group 024, 127 for Group 027 and 138 for Group 028. In between the Codex Classification and the MFDS's classification, some differences are shown. For example, the MFDS did not create a subgroup under groups of cereal grains and herbs. The MFDS classified peanuts into the nut group, though a separate group for oilseeds is present, while the Codex classified peanuts into the oilseed and oilfruit group. In addition, there is also a separate group of "plants, others" present in the MFDS's classification. Therefore, care is needed in using the Codex Classification.

생크림 위에 올려진 견과류 축제 호두 케이크

  • Kim, Jin-Mi
    • 베이커리
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    • no.8 s.457
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    • pp.128-129
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    • 2006
  • 호두 케이크는 신선하고 달콤한 생크림 케이크 위에 호두, 대추, 밀감, 파인애플이 듬뿍 올려진 선물용 케이크의 베스트 셀러이다. 부드러운 모카 제누아즈에 생크림을 얆게 샌드해 생크림을 얆게 샌드해 생크림 케이크가 부담스러운 어른들에게 가벼운 디저트 간식으로 그만이다. 케이크 위에 올려진 다양한 과일이 아이들의 입맛까지 사로잡는다.

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