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A Survey of Total Aflatoxins in Food Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Liquid Chromatography Tandem Mass Spectrometry(LC-MS/MS)  

Jang, Mi-Ran (Test & Analytical Center, Busan Regional KFDA)
Lee, Chang-Hee (Test & Analytical Center, Busan Regional KFDA)
Cho, Sung-Hye (Test & Analytical Center, Busan Regional KFDA)
Park, Joon-Shik (Test & Analytical Center, Busan Regional KFDA)
Kwon, Eun-Young (Test & Analytical Center, Busan Regional KFDA)
Lee, Eun-Jin (Test & Analytical Center, Busan Regional KFDA)
Kim, So-Hee (Test & Analytical Center, Busan Regional KFDA)
Kim, Dai-Byung (Test & Analytical Center, Busan Regional KFDA)
Publication Information
Korean Journal of Food Science and Technology / v.39, no.5, 2007 , pp. 488-493 More about this Journal
Abstract
A survey for total aflatoxins (aflatoxins $B_1$, $B_2$, $G_1$, and $G_2$) was conducted on 245 cereals and processed cereal products, and 148 nuts and processed nut products in Korea, for a total of 393 commercialized ed samples. The total aflatoxins were quantified by the immunoaffinity column clean-up method with high performance liquid chromatography (HPLC) - fluorescence detection (FLD), and were confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Total aflatoxins(AFs) were detected in 37 samples (9.4% incidence), including 2 millet samples, 1 mixed cereal (sunsik), 1 powdered malt sample, 2 processed cereal products, 6 peanut samples, 22 peanut butter samples, and 1 sample each of almonds, adlay tea, and a processed nut product. The contamination levels were $0.04-2.65{\mu}g/kg$ for aflatoxin $B_1$, and $0.04-5.51{\mu}g/kg$ for total aflatoxins. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected aflatoxins, and all 37 samples showing aflatoxins by HPLC-FLD were confirmed by LC-MS/MS.
Keywords
aflatoxins; incidence; immunoaffinity column; HPLC-FLD; LC-MS/MS;
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