• Korean Journal of Microbiology
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• v.25 no.1
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• pp.23-27
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• 1987

Low molecular weight plant ribonucleic acids including viroid-RNA molecules which are soluble in 2M lithium chloride were electrophoresed in the 0M to 8M urea-gradient polyacrylamide gel. Although the linear viroid-RNA molecules migrated at a similarrate across the urea-gradient gel under the denaturing temperature, the circular viroid-RNA molecules moved more rapidly at low urea-gradient region than at high urea-gradient region. Consequently, the migration of the circular viroid-RNA molecules showed a sudden shift across the band of linear forms in the midrange of the urea-gradient gels. Electrophoretic mobilities of the circular viroid-RNA molecules seemed to depend mainly on the concentration of urea in the denaturing urea-gradient gels.

• Journal of Life Science
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• v.29 no.12
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• pp.1408-1414
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• 2019

Enzymes are widely used in industrial applications such as detergents, food, feed production, pharmaceuticals and medical applications and major contributors to clean industrial products and processes. To screen, identify, and characterize the enzymes the zymography techniques are routinely used. The zymography technique is a simple, sensitive, and quantifiable technique that is widely used to detect functional enzymes following electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels. The method is a versatile two-stage technique involving protein separation by electrophoresis followed by the detection of enzyme activity in polyacrylamide gels under non-reducing conditions. It is based on SDS-polyacrylamide gel (PAG) copolymerization with substrates, which are degraded by the hydrolytic enzymes restored in enzyme reaction buffer after the electrophoretic separation. Any kind of biological sample can be applied and analyzed on zymography, including culture supernatants of microbes, plants extracts, blood, tissue culture fluids, enzymes in foods extracts and metaproteome. The advantage of zymography is that it is possible to directly detect the protein with activity on the electrophoretic gel as well as confirm the activity at the nanogram level. Thus, this zymography technology can be applied in various fields. However, these advantages are rather disadvantageous and can often lead to experimental errors. In this review, the advantages, disadvantages, and problem solving of zymography technique are described.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1553_1554
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• 2009

마이크로칩에서의 생체물질 분석에 있어서 재현성은 매우 중요한 사항이다. 이전부터 많은 연구자들에 의해서 분석시 재현성을 향상시키고자 많은 연구가 선행되었다. 재현성을 향상시킬 수 있는 한 방안으로 겔 전기영동이 이용되고 있다. 본 연구에서도 겔 전기영동을 마이크로칩에 접목시켜 재현성을 향상시키는 실험을 진행하였다. DNA 시료로 100bp부터 1500bp 길이의 DNA 단편들을 사용한 결과 인산완충식 염수 (PBS)만을 사용하였을 경우보다 인산완충식염수(PBS)와 5% 폴리아크릴아미드 겔 (5% polyacrylamide)과 같이 사용하였을 경우 더 향상된 재현성을 확보하였다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.6 no.1
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• pp.87-93
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• 2005

The permeability of cathodic electrolyzed water toward electrophoretic gel and dissolvability of cathodic electrolyzed water toward green tea components were compared with those of general waters in this investigation. Stained band intensities of the proteins by CBB-R prepared in cathodic electrolyzed water were compared with those in deionized water for various time intervals. Proteins were stained first by CBB-R in cathodic electrolyzed water as compared with those by CBB-R in deionized water. Moreover, cathodic electrolyzed water showed dramatically enhanced solubility toward green tea components at $25^{\circ}C$ than general waters. These results suggest much greater permeability and dissolvability of cathodic electrolyzed water than those of general waters.

• Korean Journal Plant Pathology
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• v.3 no.4
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• pp.239-244
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• 1987

Low molecular weight plant ribonucleic acids including potato spindle tuber viroid(PSTV) RNA were electrophoresed in 0M to 8M urea-gradient polyacrylamide gels. The electrophoresis was carried on in a urea - gradient gel system with 1/40 and 1/10 dilution of TBE buffer at three different temperatures, $17^{\circ}C,\;37^{\circ}C\;and\;57^{\circ}C$. The most effective separation of PSTV - RNA molecules into circular and linear forms was achieved at the highly denaturing temperature of $57^{\circ}C$ and at 1/40 dilution of TBE buffer. The electrophoretic mobility of the denatured circular viroid-RNA molecules is dependent mainly on the concentration of urea. In addition, a low concentration of TBE buffer would increase the separation distance between the circular and linear forms of PSTV-RNA molecules in the denaturing urea-gradient gel system

• Journal of Radiation Protection and Research
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• v.24 no.2
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• pp.93-99
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• 1999

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to the detection of DNA damage from a number of chemical and biological factors in vivo and in vitro. The comet assay is a novel method to assess DNA single-strand breaks, alkali-labile sites in individual cells. The effect of peach kernel extracts on radiation-induced DNA damage in human blood lymphocytes was evaluated by the SCGE assay. The lymphocytes, with or without pretreatment of the extracts, were exposed to 0, 0.1, 0.3, 0.5, 1.0 and 2.0 Gy of $^{60}Co$ gamma ray. Significantly increased tail moment, which was a marker of DNA strand breaks in the comet assay, showed an excellent dose-response relationship. The treatment of the peach kernel extracts reduced the DNA damage by 30 % in irradiated groups as compared to that in non-treated control groups. The result indicates that the extracts shows radioprotective effect on lymphocyte DNA when assessed by the comet assay.

• KSBB Journal
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• v.18 no.2
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• pp.122-126
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• 2003

Multi-cycle chromatographic separation of Iysozyme from egg white was investigated. Multi-cycle chromatography was performed by repeated cycling(one cycle: resin equilibration, sample loading, washing, elution). Two types of cation exchange resins, Cellufine CM C-200 and Bio-rex 70, were used to determine the optimum condition for the separation of Iysozyme by multi-cycle chromatography. The resin was equilibrated in 20 mM Na-phosphate buffer(pH 7.0). Chromatograms of UV absorbance levels of every cycle were compared to confirm the eluting ability of Iysozyme in the two types of gel. Collected samples from eluting regions in every cycle were assayed by 15% SDS-PAGE.

• Food Science of Animal Resources
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• v.32 no.3
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• pp.323-329
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• 2012

In order to investigate the rheological properties of myofibrillar protein (MP) mixed with tapioca starch (TS; 0, 1, and 2%) at various salt concentrations (0.1, 0.3, and 0.45 M), viscosity, gel strength, differential scanning calorimeter (DSC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were measured. Viscosity of MP increased with increasing salt concentrations (p<0.05), but not with the addition of TS. The addition of TS improved gel strength and cooking yield at all salt concentrations (p<0.05). DSC results demonstrated that the starting peak of TS gelation was observed at $55^{\circ}C$, however, no differences in peak were observed with various salt and TS levels (p>0.05). SDS-PAGE profile also showed no differences in protein bands for pork myofibrillar protein with various salt and TS levels. Based on the model study, pork model sausages with various levels of tapioca (0, 1, and 2%) and TG (1%) were manufactured. The pork model sausages with 2% TS increased pH and water holding capacity (p<0.05), while those with TGase (1%) increased most textural properties, regardless of the addition of TS. Thus, the combination of 1% TG with 2% TS improved the gel strength and water holding capacity in the meat products.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.228-230
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• 2005

The denature polyacrylamide gel stain silver nitrate is used for routine nucleic acid detection. More sensitive stains, such as Vistra Green, SYBR Green are available to address a broad range of DNA applications requiring lower detection limits in polyacrylamide gel formats. Gel Scanners, laser-scanning instruments, provide sensitive fluorescence detection of DNA gel stains. We established one step fluorescent impregnation enhanced sensitivity with simple, rapid and low cost. We have applied this fluorescent staining procedure for the routine analysis of DNA profiles generated by SSR amplification.

• Korean Journal of Food Science and Technology
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• v.14 no.2
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• pp.106-111
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• 1982

To investigate the soybean trypsin inhibitors from seven varieties of soybeans and their twenty one $F_1-hybrids$, water soluble proteins were extracted. Trypsin inhibitors were isolated from those proteins and purified by sephadex G-75 column chromatography and polyacrylamide gel electrophresis. Total 16 kinds of trypsin inhibitors were isolated. From each variety of soybeans, $5{\sim}12$ kinds of trysin inhibitors could be detected and among them, 5 kinds of trypsin inhibitors were mainly distributed.