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Rapid Detection of Fluorescent DNA on Denaturing Polyacrylamide Gel by Using Gel Scanner  

Ku Ja-Hwan (National Institute of Crop Science)
Jeong Ji-Ung (IRRI-Korea Office, National Institute of Crop Science)
Cho Young-Chan (National Institute of Crop Science)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.50, no.spc1, 2005 , pp. 228-230 More about this Journal
Abstract
The denature polyacrylamide gel stain silver nitrate is used for routine nucleic acid detection. More sensitive stains, such as Vistra Green, SYBR Green are available to address a broad range of DNA applications requiring lower detection limits in polyacrylamide gel formats. Gel Scanners, laser-scanning instruments, provide sensitive fluorescence detection of DNA gel stains. We established one step fluorescent impregnation enhanced sensitivity with simple, rapid and low cost. We have applied this fluorescent staining procedure for the routine analysis of DNA profiles generated by SSR amplification.
Keywords
polyacrylamide gel; DNA; fluorescent; silver staining; detection;
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  • Reference
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