• Title/Summary/Keyword: 검사법

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Development of Detection Method of Unapproved Genetically Modified Potato (EH92-527-1) in Korea using Duplex Polymerase Chain Reaction (Duplex PCR을 이용한 국내 미승인 유전자변형 감자(EH92-527-1)의 검사법 개발)

  • Yoo, Myung-Ryul;Kim, Jae-Hwan;Yea, Mi-Chi;Kim, Hae-Yeong
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.156-160
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    • 2013
  • A duplex polymerase chain reaction (PCR) method was developed to detect unapproved genetically modified (GM) potato (EH92-527-1) in Korea. The UDP-glucose pyrophosphorylase (UGP) gene was selected as an endogenous reference gene for potato and used to validate the specificity for 14 different crops. The primer pair EH92-F/R was designed to amplify the junction sequence between the genome and transgenic region introduced in GM potato. Its specificity was also validated using several different GM events. The detection limit of the duplex PCR method is approximately 0.05%. This duplex PCR method could be useful for monitoring cultivation of unauthorized GM potato in Korea.

Cytogenetic Radiation Adaptive Response Assessed by Metaphase Analysis and Micronuclei Test in Human Lymphocytes and Mouse Bone Marrow Cells (인체말초혈액 림프구와 마우스골수세포에서 중기염색체 분석법과 미소핵검사법을 이용한 방사선적응반응 평가)

  • Min, Jung-Jun;Bom, Hee-Seung;Lee, Seung-Yeon;Choi, Keun-Hee;Jeong, Hwan-Jeong;Song, Ho-Cheon;Kim, Ji-Yeul
    • The Korean Journal of Nuclear Medicine
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    • v.32 no.6
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    • pp.525-533
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    • 1998
  • Purpose: Radiation adaptive response in human peripheral lymphocytes and mouse bone marrow cells was investigated using both metaphase analysis and micronucleus assay. We assessed the correlation between both tests. Materials and Methods: Two groups of the human peripheral lymphocytes and mouse bone marrow cells were exposed to low dose (conditioning dose, 0,18 Gy) or high dose (challenging dose, 2 Gy) ${\gamma}$-rays. The other 4 groups were exposed to low dose followed by high dose after several time intervals (4, 7, 12, and 24 hours, respectively). The frequencies of chromosomal aberrations in metaphase analysis and micronuclei in micronucleus assay were counted. Results: Chromosomal aberrations and micronuclei of preexposed group were lower than those of the group only exposed to high dose radiation. Maximal reduction in frequencies of chromosomal aberrations were observed in the group to which challenging dose was given at 7 hour after a conditioning dose (p<0.001). Metaphase analysis and micronucleus assay revealed very good correlation in both human lymphocytes and mouse bone marrow cells (r=0.98, p<0.001 ; r=0.99, p=0.001, respectively). Conclusion: Radiation adaptive response could be induced by low dose irradiation in both human lymphocytes and mouse bone marrow cells. There was a significant correlation between metaphase analysis and micronucleus assay.

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Improvement of Ray-Tacing Performance by Using Object Pre-Proessing (오브젝트 전처리에 의한 광선추적법 속도 개선)

  • 김범수;황선태
    • Proceedings of the Korean Information Science Society Conference
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    • 1999.10b
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    • pp.565-567
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    • 1999
  • 3D 그래픽의 실사 이미지를 표현하기 위해 사용되는 광선추적법은 알고리즘상 빛과 물체의 충돌을 검사하는데 대부분의 시간이 소모된다. 보다 빠른 광선추적법을 수행하기 위해 빛과 물체의 충돌 검사시간을 줄이는 것이 요구된다. 이를 위해 본 논문에서는 디자인 시 설정된 오브젝트를 바로 랜더링 하는 방법보다 오브젝트와 카메라의 거리, 오브젝트와 월드의 상대적 크기를 고려한 바운딩 볼륨을 설정하도록 전처리해 빛과 물체의 충돌 검사 시간을 줄임으로서 광선추적법 알고리즘을 빨리 수행하는 방법을 제시한다.

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공명초음파분광법을 활용한 광컨넥터용 결합소자의 결함 평가

  • 김성훈;이길성;양인영
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.05a
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    • pp.48-48
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    • 2004
  • RUS(공명초음파분광법)는 비파괴 검사법의 하나로서 공명 주파수와 주파수 응답을 매우 정확하게 측정할 수 있으며, 탄성학적인 성질과 이방성을 결정하는데 이용 가능하다. Paul Heyliger와 Hassel Ledbetter는 steel block의 표면 크랙과 복합적층물의 내부 손상을 검출하는데 RUS를 사용하였으며, Jay G. Saxton은 RUS를 이용하여 chops, cracks, voids등을 검출하므로써 RUS의 비파괴 검사기능으로서의 가능성을 찾았다. 현재 광섬유 응용 제품에 많이 이용되고 있는 광커넥터는 초정밀 가공을 필요로 하는 중요한 부품으로서 optical fiber, ball lense로 구성되어진다.(중략)

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Comparison of One-day and Two-day Protocol of $^{11}C$-Acetate and $^{18}F$-FDG Scan in Hepatoma (간암환자에 있어서 $^{11}C$-Acetate와 $^{18}F$-FDG PET/CT 검사의 당일 검사법과 양일 검사법의 비교)

  • Kang, Sin-Chang;Park, Hoon-Hee;Kim, Jung-Yul;Lim, Han-Sang;Kim, Jae-Sam;Lee, Chang-Ho
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.2
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    • pp.3-8
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    • 2010
  • Purpose: $^{11}C$-Acetate PET/CT is useful in detecting lesions that are related to livers in the human body and leads to a sensitivity of 87.3%. On the other hand, $^{18}F$-FDG PET/CT has a sensitivity of 47.3% and it has been reported that if both $^{18}F$-FDG and $^{11}C$-Acetate PET/CT are carried out together, their cumulative sensitivity is around 100%. However, the normal intake of the pancreas and the spleen in $^{11}C$-Acetate PET/CT can influence the $^{18}F$-FDG PET/CT leading to an inaccurate diagnosis. This research was aimed at the verification of the usefulness of how much influence these two radioactive medical supplies can cause on the medical images through comparative analysis between the one-day and two-day protocol. Materials and Methods: This research was carried out based on 46 patients who were diagnosed with liver cancer and have gone through the PET/CT (35 male, 11 female participants, average age: $54{\pm}10.6$ years, age range: 29-69 years). The equipment used for this test was the Biograph TruePoint40 PET/CT (Siemens Medical Systems, USA) and 21 participants who went through the one-day protocol test were first given the $^{11}C$-Acetate PET/CT and the $^{18}F$-FDG PET/CT, the latter exactly after one hour. The other 25 participants who went through the two-day protocol test were given the $^{11}C$-Acetate PET/CT on the first day and the $^{18}F$-FDG PET/CT on the next day. These two groups were then graded comparatively by assigning identical areas of interest of the pancreas and the spleen in the $^{18}F$-FDG images and by measuring the Standard Uptake Value (SUV). SPSS Ver.17 (SPSS Inc., USA) was used for statistical analysis, where statistical significance was found through the unpaired t-test. Results: After analyzing the participants' medical images from each of the two different protocol types, the average${\pm}$standard deviation of the SUV of the pancreas carried out under the two-day protocol were as follows: head $1.62{\pm}0.32$ g/mL, body $1.57{\pm}0.37$ g/mL, tail $1.49{\pm}0.33$ g/mL and the spleen $1.53{\pm}0.28$ g/mL. Whereas, the results for participants carried out under the one-day protocol were as follows: head $1.65{\pm}0.35$ g/mL, body $1.58{\pm}0.27$ g/mL, tail $1.49{\pm}0.28$ g/mL and the spleen $1.66{\pm}0.29$ g/mL. Conclusion: It was found that no statistical significant difference existed between the one-day and two-day protocol SUV in the pancreas and the spleen (p<0.05), and nothing which could be misconceived as false positive were found from the PET/CT medical image analysis. From this research, it was also found that no overestimation of the SUV occurred from the influence of $^{11}C$-Acetate on the $^{18}F$-FDG medical images where those two tests were carried out for one day. This result was supported by the statistical significance of the SUV of measurement. If $^{11}C$-Acetate becomes commercialized in the future, the diagnostic ability of liver diseases can be improved by $^{18}F$-FDG and one-day protocol. It is from this result where tests can be accomplished in one day without the interference phenomenon of the two radioactive medical supplies and furthermore, could reduce the waiting time improving customer satisfaction.

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Application of Acoustic Emission to Weld Evaluation (용접부 검사법으로서의 Acoustic Emission)

  • 정희돈;권영각;장래웅
    • Journal of Welding and Joining
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    • v.11 no.2
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    • pp.1-12
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    • 1993
  • 용접과 관련되는 분야에서, 재료평가와 비파괴 검사법으로서의 Acoustic Emission의 응용을 살펴 보았다. 재료 평가법으로서의 AE는 다른 시험방법들이 검출해 내지 못하는 재료 내부에서의 미시적인 현상들을 정량적으로 평가할 수 있는 장점을 가지고 있다. 앞으로 새로운 용접재료의 개발이라든지, 고도의 신뢰성이 요구되는 용접부위의 정량적인 평가를 위해서 AE의 응용은 중요한 정보들을 제공하게 될 것이다. 한편 용접구조물의 비파괴적 평가법으로서의 AE는 균열의 동적인 거동 및 균열의 위치를 검지할 수 있다는 장점 때문에 응용범위는 더욱 넓어질 것이다. 여기서 유의하여야 할 점은, 목적으로 하는 AE신호를 수만배 까지 증폭시키는 과정에서 환경잡음의 영향을 무시할 수 없는 경우에는 우선적으로 잡음을 적극적으로 제거하는 고도의 연구도 함께 병행되어야 한다는 것이다.

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Evaluation of ABO Antibody Titration Using Tube and Column Agglutination Techniques (ABO 동종 응집소 역가 측정을 위한 시험관 및 미세원주응집법의 평가)

  • Cho, Chi-Hyun;Kim, Ha-Nui;Yun, Seung-Gyu;Choi, Gye-Ryung;Choi, Jae-Yeoul;Kim, Jang-Su;Lim, Chae-Seung;Kim, Young-Kee;Lee, Kap-No
    • Laboratory Medicine Online
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    • v.1 no.1
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    • pp.57-63
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    • 2011
  • Background: ABO antibody titration is useful for the evaluation of ABO-incompatible bone marrow or solid organ transplantations, yet the results quite vary between different test methods used. We compared the results of microcolumn agglutination and tube methods. Methods: Anti-A and anti-B isoagglutionin titers were determined in 63 healthy individuals (23 O, 20 A, and 20 B blood groups) using 4 different methods: immediate spin tube (tube), microcolumn agglutination without anti-human globulin (AHG) (CAT), tube with AHG (tube-AHG) and microcolumn agglutination with AHG (CAT-AHG). Results: The median (range) titers of anti-A and anti-B in group O individuals by tube, CAT, tube-AHG, and CAT-AHG methods were 64 (8-512), 64 (8-512), 128 (8-2,048), and 128 (16-2,048); 64 (16-128), 128 (16-256), 128 (16-512), and 256 (16-512), respectively. The median (range) titers of anti-A in group B and anti-B in group A individuals by the four methods were 64 (16-128), 128 (8-128), 128 (8-256), and 256 (8-256); 64 (8-128), 64 (8-128), 32 (8-128), and 64 (8-256), respectively. The isoagglutinin titer measured by CAT-AHGmethod was the highest. The titers measured by CAT and CAT-AHG methods were 0-1 titer higher than those by tube and tube-AHG methods, respectively. Whatever method was used, the isoagglutinin titers were higher in women than in men. Conclusions: CAT-AHG was the most sensitive method among the four methods tested. Since AHG titer values are critical for the clinical management and CAT has less manual procedures than tube method, CAT-AHG method could be used for the standardization of ABO antibody titration in different institutions.

A STUDY ON THE CARIES ACTIVITY TEST WITH VISIBLE LIGHT INDUCED BY LASER (가시 레이저 광을 이용한 치아우식활성검사에 관한 연구)

  • Lee, Sang-Ho;Lee, Nang-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.1
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    • pp.161-168
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    • 2000
  • The purpose of this study was to develop a practical caries activity test using laser fluorescence. The subjects of study were 30 children of $7\sim10$ years old. Fluorescence from initial carious lesion of teeth illuminated by an argon laser(488nm) was observed through barrier alter. For evaluation of accuracy and propriety of this method fer caries activity test, teeth with initial caries lesion on buccal or labial surface of children was examined with visual inspection and laser fluorescence. Visual examination for the dDfFtT and the $Cariescreen^{(R)}$ test were also done. The results from the present study can be summarized as follows: 1. Laser fluorescence test could differentiate initial caries lesions more easily than visiual inspection. 3. There was highest correlation(= 0.73) between laser fluorescent test and $Cariescreen^{(R)}$ test and. And also apparent correlation(= 0.66) exists between laser fluorescent test and caries experience measured by dDfFtT.

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Analysis of far & near distance of lateral phoria by various testing methods (검사 방법에 따른 원·근거리 수평사위의 분석)

  • Hong, Dong-Gyun;Jung, Han-Sub;Park, Sang-An
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.423-430
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    • 2004
  • This Study on three different methods which are Von Graefe test, Maddox Rod test and Howell card test of measuring far and near lateral phoria investigated. It reviewed that 420 subjects aged from 15 to 35 years old. The result were as follows; 1. Using Von Graefe lateral phoria measurement, they were measured 6% for orthophoria, 62% for exophoria, 32% for esophoria at far distance. 2. Using Von Graefe lateral phoria measurement, they were measured 2% for orthophoria, 74% for exophoria, 24% for esophoria at near distance. 3. Using Maddox Rod lateral phoria measurement, they 59% for exophoria, 37% for esophoria at far distance. 4. Using Maddox Rod lateral phoria measurement, they 67% for exophoria, 30% for esophoria at near distance. 5. Using Howell card lateral phoria measurement, they 60% for exophoria, 32% for esophoria at far distance. 6. Using Howell card lateral phoria measurement, they 69% for exophoria, 27% for esophoria at near distance.

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Common Hip Diseases and Their Imaging Studies (흔히 접할 수 있는 고관절 질환들과 영상 검사)

  • Rhyu, Kee Hyung
    • The Journal of Korean Orthopaedic Ultrasound Society
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    • v.1 no.1
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    • pp.40-49
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    • 2008
  • Hip joint is one of the most deeply located joint in human body. Moreover, most clinical symptoms of various hip diseases are vague and confusing. In these reasons, the imaging modalities of hip have been extensively studied and developed. From a plain radiograph to more complex MR arthrography, various techniques are now available. In this brief review, the long and short of imaging modalities and clinical examples of common hip diseases will be introduced. The most important significance of diagnostic imaging is its clinical relevance. It is because when we know the characteristics of the imaging modalities and the disease itself, the optimal approach to a patient can be achieved, but when we do not know, the expensive images can only be a meaningless picture.

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