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http://dx.doi.org/10.9721/KJFST.2013.45.2.156

Development of Detection Method of Unapproved Genetically Modified Potato (EH92-527-1) in Korea using Duplex Polymerase Chain Reaction  

Yoo, Myung-Ryul (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Yea, Mi-Chi (Department of plant Quarantine, Animal, Plant and Fisheries Quarantine and Inspection Agency)
Kim, Hae-Yeong (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.2, 2013 , pp. 156-160 More about this Journal
Abstract
A duplex polymerase chain reaction (PCR) method was developed to detect unapproved genetically modified (GM) potato (EH92-527-1) in Korea. The UDP-glucose pyrophosphorylase (UGP) gene was selected as an endogenous reference gene for potato and used to validate the specificity for 14 different crops. The primer pair EH92-F/R was designed to amplify the junction sequence between the genome and transgenic region introduced in GM potato. Its specificity was also validated using several different GM events. The detection limit of the duplex PCR method is approximately 0.05%. This duplex PCR method could be useful for monitoring cultivation of unauthorized GM potato in Korea.
Keywords
EH92-527-1; genetically modified potato; duplex PCR; UDP-glucose pyrophosphorylase;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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