• Korean Journal of Microbiology
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• v.39 no.4
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• pp.242-247
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• 2003

Murine encephalomyocarditis virus (EMCV) has been used as a surrogate for hepatitis A virus (HAV) for the validation of virus removal and/or inactivation during the manufacturing process of biopharmaceuticals. Recently international regulation for the validation of HAV safety has been reinforced because of the reported cases of HAV transmission to hemophiliac patients who had received ntihemophilic factors prepared from human plasma. The purpose of the present study was to compare the resistance of HAV and EMCV to various viral inactivation processes and then to standardize the HAV validation method. HAV was more resistant than EMCV to pasteurization (60oC heat treatment for 10 hr), low pH incubation (pH 3.9 at 25oC for 14 days), 0.1 M NaOH treatment, and lyophilization. EMCV was completely inactivated to undetectable levels within 2 hr of pasteurization, however, HAV was completely inactivated to undetectable levels after 5 hr treatment. EMCV was completely inactivated to undetectable levels within 15 min of 0.1 M NaOH treatment, however, residual infectivity of HAV still remained even after 120 min of treatment. The log reduction factors achieved during low pH incubation were 1.63 for HAV and 3.84 for EMCV. Also the log reduction factors achieved during a lyophilization process of antihemophilic factor VIII were 1.21 for HAV and 4.57 for EMCV. These results indicate that HAV rather than EMCV should be used for the virus validation study and the validation results obtained using EMCV should be precisely reviewed.

• Research in Plant Disease
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• v.24 no.3
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• pp.221-232
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• 2018

Rhizobacteria play important roles in plant growth and health enhancement and render them resistant to not only biotic stresses but also abiotic stresses, such as low/high temperature, drought, and salinity. This study aimed to select plant growth promoting rhizobacteria (PGPR) with the capability to mitigate biotic and abiotic stress effects on tomato plants. We isolated a novel PGPR strain, Variovorax sp. PMC12 from tomato rhizosphere. An in vitro assay indicated that strain PMC12 produced ammonia, indole-3-acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which are well-known traits of PGPR. The aboveground fresh weight was significantly higher in tomato plants treated with strain PMC12 than in non-treated tomato plants under various abiotic stress conditions including salinity, low temperature, and drought. Furthermore, strain PMC12 also enhanced the resistance to bacterial wilt disease caused by Ralstonia solanacearum. Taken together, these results indicated that strain PMC12 is a promising biocontrol agent and a biostimulant to reduce the susceptibility of plants to both abiotic and biotic stresses.

• Magazine of the Korea Concrete Institute
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• v.5 no.2
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• pp.181-188
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• 1993

In this study, a numerical method for the material nonlinear analysis of reinforced concrete shell structures including the time dependent effects due to creep and shrinkage is developed. Degenerate shell elements with the layered approach are used. The perfect or strain hardening plasticity model in compression and the linearly elastic model in tension until cracking for concrete are employed. The reinforcing bars are considered as a steel layer of equivalent thickness. Each :steel layer has an uniaxial behaviour resisting only the axial force in the bar direction. A bilinear idealization is adopted to model elasto-plastic stress-strain relationships. For the nonlinear anaysis, incremental load method combined with unbalanced load iterations for each load increment is used. To include time dependent effects of concrete, time domain is divided into several time steps which may have different length. Some numerical examples are presented to study the validity and applicability of the present method. The results are compared with experimental and numerical results obtained by other investigator.

• The Journal of the Korea Contents Association
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• v.15 no.1
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• pp.324-330
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• 2015

When diagnostic ultrasounds are used, an ultrasonic transmission media is used to effectively transmit the ultrasonic energy. Types of ingredients of polymer hydro gel, emulsion, and oil gel were compared with commercialized products by measuring characteristics of ultrasonic transmission media such as skin lubricity, skin moisturizing, and dryness speed which were contained in each different media. The mean friction coefficient measured to investigate skin lubricity showed high in the agent containing a large amount of oil, whereas the mean friction coefficient of the low viscosity emulsion did not show a significant difference with that of the polymer hydro gel. The moisture evaporation measured to investigate the dryness speed of the agent showed highest in polymer hydro gel. This showed that the larger amount of oil it had, the less moisture evaporation occurred. For skin moisture measured to investigate moisturizing characteristics of skin, low viscosity emulsion showed high in moisture content, whereas it showed slowest reduction in moisture content. As a type of emulsion appropriately containing water and oil is superior in skin moisturizing and skin tenderness, it is expected to serve as a dosage form of new ultrasonic transmission media.

• Korean journal of food and cookery science
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• v.14 no.5
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• pp.541-546
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• 1998

Volatile flavor components in leaf and petiole of fresh Pleurospermum kamtschaticum H$\_$OFFM/ were extracted by SDE (simultaneous steam distillation and extraction) method using diethyl ether as solvent. Essential oils were analyzed by gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS). Identification of volatile flavor components was based on the Rl of GC and mass spectrum of GC-MS. A total of 31 components, including 15 hydrocarbons, 4 aldehydes, 1 ketone, 5 alcohols, 2 esters, 3 acids and 1 oxide were identified in the essential oils. (Z)-${\beta}$-Farnesene, (Z, E)-${\alpha}$-farnesene and farnesene were the major volatile flavor components in fresh Pleurospermum kamtschaticum. Volatile flavor patterns of Pleurospermum kamtschaticum were analyzed using electronic nose. Sensor T30/1 and PA2 that were sensitive to alcohols had the highest resistance for fresh Pleurospermum kamtschaticum. Resistance of six metal oxide sensors was decreased in dried sample compared with fresh one.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.6
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• pp.113-123
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• 2012

The technology developed for the decrease of applying loads and self-weight of a structure is to improve conventional Foam Cement Banking Method (FCB) by applying mixed slurry of bottom ash, cement and foams. Since the foam-mixed concrete, which is a major material of the Bottom ash-mixed Light weight concrete Banking method (BLB) developed, contains mineral admixture such as cement, the behavior shows time-dependent deformation and deterioration of durability due to environmental exposure. Thus, this study is subject to figure out the characteristics of long-termed behavior and durability of the developed method by carrying out experiments for schemed parameters, which are considered to be factors affecting mainly on concrete's characteristics from mechanical analysis. As results of tests, it was found that the developed concrete offers higher resistance than conventional foamed concrete in terms of long-termed behaviors associated with drying shrinkage and creep, and durability problems of freeze-thaw and carbonation processes, especially with addition of bottom ash.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.114-114
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• 2018

탄소 재료는 뛰어난 물성에서 다양한 재료로의 응용이 기대되고 있다. 특히, 이종 원소 함유 카본 재료는 전기적 특성과 촉매성의 발현 등 새로운 기능을 카본 재료에 부여할 수 있어서 연료 전지, 에너지 저장, 태양 전지 등에의 응용이 기대되고 있다. 최근, 용액 중의 저온 플라즈마인 솔루션 플라즈마(solution plasma process)를 이용하여 벤젠 용액 등에서 탄소 재료 합성에 성공하였다. 그러나 기존의 연구에서는 솔루션 플라즈마 프로세스를 이용하여 합성한 이종원소 카본은 전도성이 낮아 이종원소의 함유량을 낮추는 고온의 열처리가 필요했다. 따라서 본 연구에서는 우수한 물리적 전기적 특성을 갖는 그래핀(graphene)과 같은 이종 원소 카본 나노시트(heteroatom carbon nanosheets)의 합성 및 메커니즘(mechanism)에 대해 검토하였다. 다양한 이종원소를 포함한 유기용매 안에 바이폴라 펄스 전원에 의한 전압을 두 텅스텐 전극 간에 인가하고, 솔루션 플라즈마를 생성하여 이종원소 카본 재료를 합성했다. 플라즈마 생성은 텅스텐 봉을 전극으로 사용하고 전압을 2.0 kV, 펄스 주파수를 200 kHz, 펄스 폭을 $1.0{\mu}s$, 전극 간 거리를 1.5 mm에서 일정하게 유지하며 200 mL 유기용매 중에서 방전시키는 것으로 재료를 합성했다. 플라즈마 방전 후, 필터을 이용하여 흡인 여과한 뒤 $200^{\circ}C$에서 1 시간 동안 건조 시켰다. 건조 후의 이종원소 카본의 물리적 특성을 원소 분석, X선 회절 법(XRD), 저항률 측정, 투과형 전자 현미경(TEM) 및 라만 분광법, 전자 현미경(SEM), X-선광전자분광기(XPS)등을 이용하여 카본의 형상 및 특성을 분석하였다. 그 결과 다양한 이종원소를 포함한 유기용매 중 2-pyrrolidone을 사용했을 때, 이종 원소 카본 나노시트를 합성하는데 성공하였다. 또한, 이 연구방법을 통해서 솔루션 플라즈마 프로세스를 통한 카본 나노시트 합성의 메커니즘을 규명하였다.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.8
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• pp.82-88
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• 2008

This paper describes the influence of the bridge formed between electrodes by electrolyte for tracking process. Electrolytes is made by IEC(International Electrotechnical Commission) 60589, NaCl added to deionized water as each 1, 3, 5[wt%]. The used test equipment is made according to KS(Korean Industrial Standard) C IEC 00112. It is investigated voltage, current, value of resistance and thermal image when bridge formed between electrodes on tracking process. As a result, as conductivity of electrolyte gets bigger as Joule's heat on bridge also gets bigger. But It is not over electrolyte's boiling point due to evaporation heat of electrolyte. However as conductivity of electrolyte gets bigger as the necessary time of dry band gets shorter. So dry band is existed more long time between ahead of droplet to next droplet and discharge chance at dry band gets much. Therefore tracking process gets faster.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.2
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• pp.75-86
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• 2012

This paper presents some research results on the shrinkage characteristics and frost resistance before and after cracking of FR-ECC(Fire Resistance-Engineered Cementitious Composite). Also, a waterstop performance and exfoliating resistance of multi-layer lining specimens using FR-ECC and flexural performance of beam member by repaired FR-ECC are estimated in this paper. Experimental results indicate that the plastic shrinkage crack and length change ratio of FR-ECC have been reduced as compared with that of the existing repair mortar, and that its crack resistance on the dry shrinkage is improved under the confining stress. As well as FR-ECC has been great in the frost resistance and its tensile properties under the cracked state have been not reduced by freezing and thawing reaction. In addition, beam member by repaired FR-ECC have been increased in the flexural properties such as initial crack moment, yeild moment, and its crack width has been controled in a stable by the frexural failure.

• Journal of the Korean Geosynthetics Society
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• v.13 no.3
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• pp.21-30
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• 2014

In this study, the applicability of the lightweight dynamic cone penetrometer in the domestic slope site was investigated using the weathered granite soil sampled form the Namwon slope site. And then, the lightweight dynamic cone penetration tests according to the change in the degree of compaction and water content were performed and it was analyzed with the correlations between the degree of compaction, the void ratio, the degree of saturation and the value of cone resistance. From the laboratory test results, the cone penetration resistance was rapidly increased in the dry side of the optimum moisture content, and it was largely decreased in the wet side of the optimum moisture content. Moreover, when the degree of compaction and the degree of saturation are large, the cone resistance is increased linearly. And a high correlativity was shown between water content, void ratio, the degree of saturation and the cone resistance. From these results, it is judged that the lightweight dynamic cone penetrometer can be applied to the investigation on the site slope.