• Journal of Life Science
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• v.27 no.10
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• pp.1111-1120
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• 2017

This study was conducted to select pepper lines that were tolerant to excessive water injury among the pepper germplasm and investigate the genetic characteristics of those lines to contribute to the breeding of pepper cultivars with stable productivity in abnormal weather. Each of the tolerant and susceptible lines went through immersion treatment, and differentially expressed genes between them were analyzed. The tolerant line showed increased expression of the CA02g26670 gene, which is involved in the CONSTANS protein pathway and regulation of flowering by day length, but it exhibited decreased expressions of CA01g21450, CA01g22480, CA01g34470, CA02g00370 and CA02g00380. The susceptible line showed increased gene expressions of CA02g09720, CA02g21290, CA03g16520, CA07g 02110, and CA12g17910, which are involved in the inhibition of proteolytic enzyme activity, DNA binding, inhibition of cell wall-degrading enzyme, and inhibition of nodulin, respectively. Meanwhile the expressions of CA02g02820, CA03g21390, CA06g17700 and CA07g18230 decreased in the susceptible line, in relation to calcium-ion binding, high temperature, synthesis of phosphocholine and cold stress, respectively. The expressions of genes related to apoptosis and peroxidase increased, while that of CA02g16990, which functions as a nucleoside transporter, decreased in both the tolerant and susceptible lines. Based on the different gene expressions between the tolerant and susceptible lines, further studies are needed on breeding abiotic stress-tolerant lines.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.66-73
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• 2013

This study was conducted to determine the effects of rice hull cover on seed germination and tray types, soil types, shading conditions for seedling growth of Codonopsis pilosuala. We also examined the feasibility of cultivation of small seedlings transplanted in the Gyeonggi-do area for a month. Under control condition, the seed germination was 8% whereas it dramatically increased to 78% under rice hull cover treatment. Under the different conditions (tray types, soil types, and shading conditions), young seedlings showed the best quality without shading in TKS soil of 50 plug cell tray, with the growth characteristics of plant height (11.9 cm), number of leaves (71), leaf width (3.1 cm), leaf length (2.6 cm), and root length (14.3 cm). Seedling quality was the best without any shading in TKS+perlite, with the physiological characteristics of evaporation (3.9 $mmol{\cdot}m^{-2}s^{-1}$), carbon assimilation (9.1 ${\mu}mol{\cdot}m^{-2}s^{-1}$), and water use efficiency (2.2 ${\mu}mol{\cdot}m^{-2}s^{-1}$). Considering the economical, morphological, physiological and survival rate of the seedlings, it was an ideal method for transplanting seedlings in the field after they have been grown for 30-45 days in TKS and TKS+perlite of 200 plug cell tray in 0% or 30% shaded conditions. As the results of 5 months examination on the possibility to cultivate Codonopsis pilosuala in the Gyeonggi-do area, 88% to 96% of survival rate was observed with normal induced flowers. Therefore, cultivation of Codonopsis pilosuala was possible in the Gyeonggi-do area. While there were symptoms of etiolation and wilting under no shading condition, they did not appear in 30% and 70% shading conditions. Therefore, cultivation of Codonopsis pilosuala is considered to be necessary 30% or 70% shading.

• The Journal of Natural Sciences
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• v.8 no.1
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• pp.71-80
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• 1995

This experiment was conducted to investigate the effect of raising seedling methods of cold water, low night temperature, and abandoned mine on forcing culture of strawberry. The results were as follows ;1. $17^{\circ}C$ cold water alone and cold water plus 8-hour short day treatments significantly accelerated the flower bud differentiation of Nyoho cultivar to 11 and 15 days, respectively, comparing to common soil raising seedling.2. The date of flower bud differentiation of Nyoho cultivar as influenced by cold water treatment the 17th, the 13th and the 10th of September, respectively, when the seedlings were treated on the 10th of September and on the 25th and the 10th of August in 1991. In 1992, data showed that the flower bud differentiation dates were the 4th and the 2nd of September and the 29th of August when cold water treatment was done on the 10th of August and on the 25th and the 10th of July.3. The first harvest date when Nyoho cultivar was treated by cold water for 30days from Jul. 25 was Nov. 10, this implying that the harvesting day could advanced to 19 days comparing to that by the common soil raising seedling method. The resulting yield was recorded to 21.94 ton per hectare.4. Regardless of the starting date of the treatment, $13^{\circ}C$ low night temperature plus 8-plus short day treatments for 20 days required 17 days to differentiate the flower bud of Nyoho cultivar. Harvesting day could be advanced to 59 days comparing to that by the common raising seedling method when the seedling was treated on July 25, this resulting in increasing the total yield obtained by April 14 of following year to 8.25 ton per hectare.5. When the seedlings of both Nyoho and Hokowase cultivars were raised under the condition of abandoned mine, flowering and harvest date were earlier by the treatment for 20 days than that for 30 days or 40 days. The highest yiesd obtained was recorded in Nyoho cultivar or 1.88 ton per hectare.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.42-52
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• 2004

The purpose of this study was to propose the change of green area and green structure according to the change of the Floor Area Ratio(FAR) of apartment complex. The survey site was selected two each of apartment complex after due consider three type that is, the FAR below 100％, the FAR below 250％ and the FAR over 250％. Green rate is 40.23～44.23％, green width is 3～15m, green volume is 1.06～1.71㎥／$m^2$, planting density of cannopy layer is 0.04～0.06tree／$m^2$, planting density of understory layer is 0.06～0.20tree／$m^2$ in case of Hwagok 2nd Jugong apartment(FAR 99％) and Gaepo Jugong apartment(FAR 73％) constructed in the 1970～1980. Green rate is 24.16～26.16％, green width is 3～7m, green volume is 0.84～1.54㎥／$m^2$, planting density of cannopy layer is 0.18～0.31tree／$m^2$, planting density of understory layer is 0.15～0.35tree／$m^2$ in case of Deungchon Jugong aparment(FAR 226％) and Gaehwa apartment(FAR240％) construsted in the mid-1990's. Green rate is 23.47～25.53％, green width is 2～6m, green volume is 1.06～2.12㎥／$m^2$, planting density of cannopy layer is 0.25tree／$m^2$, planting density of understory layer is 0.22～0.37tree／$m^2$ in case of Hanbo Guam apartment(FAR 269％) and Donga 3rd apartment(FAR 376％) constructed in the last-1990's. As the results of analysis of green structure according to changes of the Floor Area Ratio(FAR), the greater the FAR, the higher the rate of green area. The green volume and planted density is low without the change of the FAR and planted structure was simple-layer structure composed of alien woody species.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.3
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• pp.132-141
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• 2012

Climate change impact assessment of cereal crop production in South Korea was performed using land attributes and daily weather data at a farm scale as inputs to crop models. Farmlands in South Korea were grouped into 68 crop-simulation zone units (CZU) based on major mountains and rivers as well as existing land use information. Daily weather data at a 1-km grid spacing under the A1B- and RCP8.5 scenarios were generated stochastically to obtain decadal mean of daily data. These data were registered to the farmland grid cells and spatially averaged to represent climate conditions in each CZU. Monthly climate data for each decade in 2001~2100 were transformed to 30 sets of daily weather data for each CZU by using a stochastic weather generator. Soil data and crop management information for 68 CZU were used as inputs to the CERES-rice, CERE-barley and CROPGRO-soybean models calibrated to represent the genetic features of major domestic cultivars in South Korea. Results from the models suggested that the heading or flowering of rice, winter barley and soybean could be accelerated in the future. The grain-fill period of winter barley could be extended, resulting in much higher yield of winter barley in most CZUs than that of rice. Among the three major cereal grain crops in Korea, rice seems most vulnerable to negative impact of climate change, while little impact of climate change is expected on soybeans. Because a positive effect of climate change is projected for winter barley, policy in agricultural production should pay more attention to facilitate winter barley production as an adaptation strategy for the national food security.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.80-85
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• 2020

A new Gomchwi cultivar 'Soomany' was derived from a cross between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The investigation and selection of growth and yield characteristics were conducted from 2007 to 2015 in field and greenhouse of Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, in Korea. On a newly developed cultivar 'Soomany', color of petiole ear is light green, petiole trichome is existent, trichome and light on the back of the leaves don't exist, and density of leaf vein is degree 4. Plant height, leaf length, leaf width and petiole length are 77.1, 22.3, 21.5 and 57.2 cm, respectively in the growth characteristics of the 2_nd year. Plant size was also higher than that of 'Sammany' generally. Bolting date was on August 15. Flowering date was on September 19, about 9 days later than 'Sammany'. 'Soomany' and 'Sammany' had 149 and 133 leaves per plant, respectively. Total yield of 'Soomany' (1,623 g/plant) made a very good comparison with that of 'Sammany' (1,385 g/plant). 'Soomany' showed harder leaves (25.8 ㎏/㎠) than 'Sammany' (20.8 ㎏/㎠), whereas 'Soomany' had thinner leaves (0.53 mm) than 'Sammany' (0.62 mm). 'Soomany' variety has shown strong resistance to powdery mildew disease compared to 'Sammany'. In May 2019, the right of variety protection of 'Soomany' was registered as a new Gomchwi variety (Register No. 192).

• Journal of agricultural medicine and community health
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• v.23 no.2
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• pp.275-285
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• 1998

Antibody responses(IgG) to Paragonimus westermani. Clonorchis sinensis, Cysticercus cellulosae, Sparganum Anisakis simplex, Toxocara canis and Trichinella spiralis were studied. The ELISA technique was performed to determine the prevalence of above helminthic diseases. 975 cases obtained from Yanbian of China during October, 1995 to July, 1997 were examined with a positive antibody titer of 5.74% in clonorchiasis, 4.92% in paragonimiasis, 1.54% in cysticercosis. 8.51% in sparganosis, 1.85% in anisakiosis, 12.51% in toxocariasis, and 7.08% in trichinosis respectively. And 23.87% in showed positive antibody titer at least one of the seven helminths. The differences of the age and sex in the positive sera were analysed by the Chi-squared test and the level of significance accepted was p<0.05. The significant differences in positive antibody production were P.W.(p<0.01). C.S.(p<0.01), A.S.(p<0.05). T.C.(p<0.001), and T.S.(p<0.01) respectively in age groups. sparganosis(p<0.05) in sex groups. Other parasites showed that there were no significant differences among age groups and sex groups(males and females). Higher positive antibody rate of C.S. and P.W. occured in the 50-59 years old and those of T.C. and T.S. happened in the 20-29 years old. Patients of internal disease showed more positive antibody titer, that is to say, there was significant difference between positive rate of internal diseases and that of control (p<0.01. p<0.05) in 6 helminths except cysticercosis. The result showed that some cross reactions existed among nematodes, and the developed techniques(EITB) should be done for a correct diagnosis. Also the prevalence of some important helminths may be evaluated from the result and it would be a basic data for controlling parasitic diseases in Yanbian.

• Journal of Plant Biology
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• v.27 no.3
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• pp.179-189
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• 1984

Durch vergleichende Untersuchungen von Ribes nigrum L. var. 'Rosenthals Langtraubige Schwarze' zwischen $prim\"{a}rem$ und $sekund\"{a}rem$ Jugendstadium (P.J. und S.J.) eine vertiefte Einsicht in die beiden zeitlich unterschielich langen $Entwicklungsabl\"{a}ufe$ zu bekommen und auf diese Weise bessere Ansatzpunke $f\"{u}r$ die Regulierung der $Bl\"{u}tc-nknospeninduktion$ zu gewinnen, war das Ziel vorliegenden Untersuchungen. Diese Untersuchungen $f\"{u}hrten$ zu den flogenden Ergebnissen: Die Dauer der Jugendphase von Ribes nigrum L. betrug bei den P.J.-Pflanzen ca. 7 Monate, bei den S.J.-Pflanzen ca. 5 Monate. Die $Bl\"{u}hreife$-(Ripeness to flower)-setzte bei den P.J.-Pflanzen erst beim 20. Nodium ein, bei den S.J.-Pflanzen bereits beim 5. Nodium. Das $f\"{u}hrte$ dazu, da${\beta}$ bei gleicher Nodienzahl die P.J.-Pflanzen weniger $Bl\"{u}te-nknospen$ angesetzt hatten als die S.J.-Pflanzen. Die erste $Bl\"{u}tenknospendifferenzierung$ setzte bei den P.J.-Pflanzen in der oberen Triebmitte etwa am 30. Nodium ein, beiden S.J.-Pflanzen bereits in der unteren Tricbmitte etwa am 20. Nodium. Bie den P.J.-Pflanzen waren die untersten 20. Nodien immer steril, $w\"{a}hrend$ bei den S.J.-Pflanzen $h\"{a}uflgsten$ an dem ersten Nodium $\"{u}ber$ der Basis fertil waren. In allen Versuchen hat sich gezeigt, da${\beta}$ der Wachstumsabschlu${\beta}$ -(Bildung einer Terminal Knospe)-, der bei Ribes nigrum L. durch Kurztag eingeleitet wird, bei den P.J.-Pflanzen langsammer als bei den S.J.-Pflanzen erfolgt. Dies $f\"{u}hrt$ dazu, da${\beta}$ die Pflanzen der $sekund\"{a}ren$ Jugendphase auf die photokybernetischen Stimulation empfindlicher reagieren als die der $prim\"{a}ren$ Jugendphase. Alle untersuchten Versuchsdaten $f\"{u}hren$ zu dem Schlu${\beta}$, da${\beta}$ die Wurzelgibberelline $(GA_n)$ keinen Einflu${\beta}$ auf die $Jugendsterilit\"{a}t$ ($Basissterilit\"{a}t$) haben. Aus den Untersuchungen geht hervor, da${\beta}$ die induktive Ma${\beta}$nahme zur Beschleunigung der $Bl\"{u}tenknospenbildung$ $f\"{u}r$ eine $Z\"{u}chtungsselektion$ erst dann eingesetzt werden sollen, wenn die P.J.-Pflanzen mehr als 20 Nodien ausgebildet haben.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.5
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• pp.279-284
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• 2008

This study was performed to investigate effect of different soil managements on physical properties and microbial activities in volcanic ash citrus orchard soil. Experiment plots had managed to control weeds on soil for 4 years with clean cultivation (CCM) used with herbicide, natural sod cultivation (NSCM), kentucky blue grass sod cultivation (KBG). Soil samples were taken on October, in both 1998 and 2000 from 3 experimental plots. In NSCM, Soil hardness was lower at 11.8 mm than in CCM. And water stable Aggregation coefficient(>0.5 mm) was high at 26.7% compared with CCM. Soil bulk density and porosity showed no significant among the treatments. Soil acid phosphatase was high in sod cultivation plots and the amount of microbial biomass C was about twice higher at $525.4mg\;kg^{-1}$ in KBG than in CCM. Conclusionally, Sod cultivation improved soil physical properties such as aggregation, hardness and increased microbial activities compared with clean cultivation in citrus orchard soil. Soil total PLFA, acid phosphatase, and microbial biomass C contents were investigated on May in nonvolcanic ash citrus soil. Soil samples were collected at 5 sites each; convention cultivation grown with herbicide, natural sod cultivation grown with 1/2 chemicals, organic cultivation. That sites have been managed for 5 years over. PLFA contents were two times higher at $112.2n\;mol\;g^{-1}$ in organic cultivation than in convention cultivation. According to the PLFA indicator, Gram negative bacteria and actinomycetes in organic cultivation were high compared with convention cultivation, which were at 15.1%, 6.6%, respectively. Soil microbial biomass C contents was about twice higher in organic cultivation than in convention cultivation. Soil acid phosphatase was high at 17.6% in organic cultivation compared with convention cultivation.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.368-373
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• 2008

In order to investigate the effects of low temperature pretreatment of floral bud and plant growth regulators on anther-derived callus and shoot differentiation, anthers were cultured on 1/2 MS medium supplemented with 2,4-D, NAA, BA and TDZ. This plant depends on the plant growth regulators, for these anthers couldn't respond on 1/2 MS medium without plant growth regulators. 2,4-D was a prerequisite substance in this experiment, especially 52.6% of callus formation on MS medium with 2.0mg/L 2,4-D alone. However, the optimum medium was on 1/2 MS medium with 0.1 mg/L 2,4-D and 1.0mg/L BA for continuous growth and shoot differentiation from the anther. Calli derived from on MS medium with 2.0mg/L 2,4-D transferred to the 1/2MS medium with TDZ and BA. TDZ were less superior to BA, only one anther could produce shoot on MS media with 1.0mg/L TDZ. On the other hand, when the calli transferred to the medium with 3.0mg/L BA, adventitious shoots were proliferated, subsequently, regenerated shoots elongated from the embryogenic calli. After floral buds of one week before anthesis were incubated at $5^{\circ}C$ refrigerator for eight or fifteen days, anthers seperated from floral buds were cultured on 1/2MS medium supplemented with 0.1mg/L 2,4-D and 1.0mg/L BA. Callusing and shoot differentiation on anthers from treated at $5^{\circ}C$ for eight days were more effective than those of fifteen days or control.