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http://dx.doi.org/10.5352/JLS.2017.27.10.1111

Study on the Genetic Characteristics of Waterlogging Tolerant Pepper (Capsicum annuum L.) for Breeding Tolerant Varieties against Flooding Stress  

Yang, Eun Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Chae, Soo-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Hong, Jong-Pil (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Hye-Eun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Park, Eun Joon (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Moon, Ji-hye (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Park, Tae-Sung (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Roh, Mi-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Ok Rye (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Sang Gyu (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dae Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Lee, Sun Yi (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Myeong Cheoul (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Journal of Life Science / v.27, no.10, 2017 , pp. 1111-1120 More about this Journal
Abstract
This study was conducted to select pepper lines that were tolerant to excessive water injury among the pepper germplasm and investigate the genetic characteristics of those lines to contribute to the breeding of pepper cultivars with stable productivity in abnormal weather. Each of the tolerant and susceptible lines went through immersion treatment, and differentially expressed genes between them were analyzed. The tolerant line showed increased expression of the CA02g26670 gene, which is involved in the CONSTANS protein pathway and regulation of flowering by day length, but it exhibited decreased expressions of CA01g21450, CA01g22480, CA01g34470, CA02g00370 and CA02g00380. The susceptible line showed increased gene expressions of CA02g09720, CA02g21290, CA03g16520, CA07g 02110, and CA12g17910, which are involved in the inhibition of proteolytic enzyme activity, DNA binding, inhibition of cell wall-degrading enzyme, and inhibition of nodulin, respectively. Meanwhile the expressions of CA02g02820, CA03g21390, CA06g17700 and CA07g18230 decreased in the susceptible line, in relation to calcium-ion binding, high temperature, synthesis of phosphocholine and cold stress, respectively. The expressions of genes related to apoptosis and peroxidase increased, while that of CA02g16990, which functions as a nucleoside transporter, decreased in both the tolerant and susceptible lines. Based on the different gene expressions between the tolerant and susceptible lines, further studies are needed on breeding abiotic stress-tolerant lines.
Keywords
Abiotic stress; Capsicum annuum; DEG; gene annotation; tolerant lines;
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1 Win, P. P., Kyi, P. P. and Waele, D. D. 2011.Effect of agroecosystem on the occurrence of the rice root-knot nematode Meloidogyne graminicola on rice in Myanmar. Australas. Plant Pathol. 40, 187-196.   DOI
2 Yoon, J. B. and Park, H. G. 2005. Trispecies bridge crosses, (Capsicum annuum $\times$ C. chinense) $\times$ C. baccatum, as an alternative for introgression of anthracnose resistance from C. baccatum into C. annuum. J. Kor. Hort. Sci. 46, 5-9.
3 Zhou, M. X., Li, H. B. and Mendham, N. J. 2007. Combining ability of waterlogging tolerance in barley. Crop Sci. 47, 278-284.   DOI
4 Colmer, T. D., Gibberd, M. R., Wiengweera, A. and Tinh, T. K. 1998. The barrier to radial oxygen loss from roots of rice (Oryza sativa L.) is induced by growth in stagnant solution. J. Exp. Bot. 49, 1431-1436.   DOI
5 Cornelious, B., Chen, P., Chen, Y., de Leon, N., Shannon, J. G. and Wang, D. 2005. Identification of QTLs underlying water-logging tolerance in soybean. Mol. Breed. 16, 103-112.   DOI
6 Denance, N., Szurek, B. and Noel, L.D. 2014. Emerging functions of nodulin-like proteins in non-nodulating plant species. Plant Cell Physiol. 55, 469-474.   DOI
7 Food and Agriculture Organization-World Health Organization. (FAO/WHO) 2016. Food and agriculture data. http://www.fao.org/faostat/en/#data.
8 Githiri, S. M., Watanabe, S., Harada, K. and Takahashi, R. 2006. QTL analysis of flooding tolerance in soybean at an early vegetative growth stage. Plant Breed. 125, 613-618.   DOI
9 Kim, B. S., Kwon, T. R., Hwang, J. E., Lee, J. M., Park, D. G., Ahn, J. H. and Kim, H. Y. 2010. Resistance to phytophthora blight of commercial pepper cultivars in Korea. Res. Plant Dis. 16, 141-147.   DOI
10 Huang, D. W., Sherman, B. T. and Lempicki, R. A. 2009. Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nat. Protoc. 4, 44-57.   DOI
11 Kim, D. H., Cho, M. C., Chae, Y., Cho, Y. S. and Shin, Y. A. 2005. The Selection of Phytophthora capsici Resistant Lines for Pepper (Capsicum spp.) Breeding. Kor. J. Hort. Sci. Technol. 23, 42-42.
12 Lee, J. H., Trenholm, L. E., Unruh, J. B. and Hur, J. H. 2006. Sensor-based technology for assessing drought stress in two warm-season turfgrasses. Kor. J. Turfgrass Sci. 20, 213-221.
13 Brugiere, N., Jiao, S., Hantke, S., Zinselmeier, C., Roessler, J. A., Niu, X. and Habben, J. E. 2003. Cytokinin oxidase gene expression in maize is localized to the vasculature, and is induced by cytokinins, abscisic acid, and abiotic stress. Plant Physiol. 132, 1228-1240.   DOI
14 Chae, S. Y., Yang, E. Y., Lee, W. M., Cho, M. C., Choi, H. S., Kim, K. T. and Jeong, H. B. 2009. Evaluation of characteristics for high red pigments contented pepper variety breeding. Kor. J. Hort. Sci. Technol. 27, 76-76.
15 Chae, S. Y., Yang, E. Y., Won, J. H., Choi, H. S., Lee, W. M., Kim, S., Park, D. G. and Yoon, M. K. 2013. Germplasm evaluation and selection for breeding of resistant rootstock varieties to bacterial wilt in pepper (Capsicum annuum L.). Kor. J. Hort. Sci. Technol. 31, 92-92.
16 Lewit-Bentley, A. and Rety, S. 2000. EF-hand calcium-binding proteins. Curr. Opin. Struct. Boiol. 10, 637-643.   DOI
17 Chen, C., Edelstein, L. C. and Gelinas, C. 2000. The Rel/NF-${\kappa}B$ family directly activates expression of the apoptosis inhibitor Bcl-xL. Mol. Cell. Biol. 20, 2687-2695.   DOI
18 Choi, S. M. and Lee, J. M. 2005. Evaluation of hot pepper genotypes for selection of phytophthora disease resistant rootstocks. Kor. J. Hort. Sci. Technol. 23, 149-149.
19 Lee, O. H., Hwang, H. S., Kim, J. Y., Han, J. H., Yoo, Y. S. and Kim, B. S. 2001. A search for sources of resistance to powdery mildew (Leveillula taurica (Lev.) Arn) in pepper (Capsicum spp.). Kor. J. Hort. Sci. Technol. 19, 7-11.
20 Lo, S. F., Yang, S. Y., Chen, K. T., Hsing, Y. I., Zeevaart, J. A., Chen, L. J. and Yu, S. M. 2008. A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice. Plant Cell 20, 2603-2618.   DOI
21 Oh, S. K., Yoon, J. S., Choi, G. J., Jang, H. A., Kwon, S. Y. and Choi, D. I. 2013. Capsicum annuum homeobox 1 (CaHB1) is a nuclear factor that has roles in plant development, salt tolerance, and pathogen defense. Biochem. Biophys. Res. Commun. 442, 116-121.   DOI
22 Pae, D. H., Chae, Y., Wang, T. C., Engle, L. M. and Shanmugasundaram, S. 2006. Selection of new breeding materials with resistance to anthracnose in Capsicum annuum. 27th International Horticultural Congress & Exhibition. 419-419.
23 Pathan, M. S. and Sleper, D. A. 2008. Advances in soybean breeding. In Genetics and genomics of soybean. Springer. New York. 113-133.
24 Rhee, D. A and Park, S. K. 1975. The effect of soil moisture level on flowers and fruits drop of red pepper. J. Kor. Soc. Hort. Sci. 16, 99-105.
25 Stolzy, L. H. and Sojka, R. E. 1984. Effects of flooding on plant disease. In: Flooding and plant growth. Academic Press. 221-264.
26 Rossi, J. M. and Lindquist, S. 1989. The intracellular location of yeast heat-shock protein 26 varies with metabolism. J. Cell Biol. 108, 425-439.   DOI
27 Rushton, P. J., Somssich, I. E., Ringler, P. and Shen, Q. J. 2010. WRKY transcription factors. Trends Plant Sci. 15, 247-258.   DOI
28 Smith, D. D., Summers, P. S. and Weretilnyk, E. A. 2000. Phosphocholine synthesis in spinach: Characterization of phosphoethanolamine N-methyltransferase. Physiol. Plantarum. 108, 286-294.   DOI
29 Trapnell, C., Pachter, L. and Salzberg, S. L. 2009. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics 25, 1105-1111.   DOI
30 Trapnell, C., Williams, B. A., Pertea, G., Mortazavi, A. M., Kwan, G., van Baren, M. J., Salzberg, S. L., Wold, B. and Pachter, L. Transcript assembly and quantification by RNASeq reveals unannotated transcripts and isoform switching during cell differentiation. Nat. Biotechnol. 28, 511-515.   DOI
31 Valverde, F., Mouradov, A., Soppe, W., Ravenscroft, D., Samach, A. and Coupland, G. 2004. Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science 303, 1003-1006.   DOI
32 Van Toai, T. T., St. Martin, S. K., Chase, K., Boru, G., Schnipke, V., Schmitthenner, A. F. and Lark, K. G. 2001. Identification of a QTL associated with tolerance of soybean to soil waterlogging. Crop Sci. 41, 1247-1252.   DOI
33 Wilkes, D. E., Rajagopalan, V., Chan ,C. W., Kniazeva, E., Wiedeman, A. E. and Asai, D. J. 2007. Dynein light chain family in tetrahymena thermophila. Cell Motil. Cytoskeleton 64, 82-96.   DOI