• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.22 no.1
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• pp.31-44
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• 2017

Pfiesteria piscicida is one of heterotrophic dinoflagellate having toxic metaboliges, and it is difficult to detect and quantify this dinoflagellate via light microscope due to small size and morphological similarity with Pfiesteria-like dinoflagellate (PLD) species. Alternatively, we developed quantitative real-time PCR assay based on EvaGreen and determined field accessibility throughout the investigation of distribution in the entire Korean coastal waters and population dynamics in Shihwa Lake. The P. piscicida-specific primers based on internal transcribed spacer 1 (ITS 1) were designed and the specificity of primers was confirmed by PCR with other genomic DNAs which have genetic similarity with target species. Through real-time PCR assay, a standard curve which had a significant linear correlation between log cell number and $C_T$ value ($r^2{\geq}0.999$) and one informative melting peak ($88^{\circ}C$) were obtained. These results implies that developed real-time PCR can accurately detect and quantify P. piscicida. Throughout the field applications of real-time PCR assay, P. piscicida was distributed in western (Mokpo and Kimje) and easthern (Gangneng) Korean coastal water even though light microscopy failed to identify P. piscicida. In the investigation of population dynamics in Shihwa Lake, the density of P. piscicida was peaked in June, July and August 2007 at St. 1 where salinity (${\leq}15psu$) was lower than the other 2 sites. In this study, we successed to develop EvaGreen bassed real-time PCR for detection and quantification of P. piscicida in fields, so this developed assay will be useful for various ecological studies in the future.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.14 no.4
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• pp.213-228
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• 2009

This study was conducted to investigate spatio-temporal changes in macrobenthic community structure and benthic environmental conditions in Geoje-Hansa Bay, which is the greatest oyster producing site in Korea. Field survey for benthic environment and macrobenthos was seasonally carried out at 15 stations covering oyster farming sites and non-farming sites from February to November, 2008. The grain size of surface sediments was dominated by very fine silt with the mean phi of about $9\;{\Phi}$ and TOC was 1.9% on average. Mean dissolved oxygen content was 8.1 mg/L and lowest in August corresponding to the 2nd degree in seawater quality criteria. Total species number was 351 and mean density was $3,675\;ind./m^2$, both of which were dominated by polychaete worms. Spatio-temporal variation in above two biological variables was great with higher values seasonally in spring and spatially in channels rather than inner bay. Dominant species were Lumbrineris longifolia (21.3%), Aphelochaeta monilaris (17.8%) and Ericthonius pugnax(6.1%), all of which are typical species of organically enriched area. From the multivariate analyses, the whole macrobenthic community was distinguished into two groups of channel and inner bay group. Spatio-temporal changes of macrobenthic community in Geoje-Hansan Bay were related to those of TOC and acid volatile sulfide (AVS). Our results showed that Geoje-Hansan Bay should be intermediately affected by organic pollution, and that such organic enrichment was more remarkable at farming stations in the inner bay.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.2
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• pp.98-104
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• 2008

In April, July, October and December, 2003, we investigated the effects of water temperature, salinity and extinction coefficient on the distribution of phytoplankton communities at 22 stations in Jinju Bay of Korea. Water temperature and salinity showed a wide range of $10.4^{\circ}C-21.8^{\circ}C$ and 4.34-33.21 psu. Extinction coefficients showed a range of 0.09-3.08, above 1 from almost all the areas except in some central areas, especially, showed highest value (>2) in the estuary area. In phytoplankton, a total of 95 species belonging to 51 genera were identified. The predominant species were mainly diatoms throughout the year. Dominant species was Thalassionema nitzschioides, Skeletonema costatum, Thalassiosira sp. in April, S. costatum, Leptocylindrus danicus in July, C. debilis, S. costatum, C. curvisetus, Pseudonitzshia pungens in October, S. costatum, Asterionellopsis glacialis and C. debilis in December. S. costatum was a major dominant species for all the seasons. Considering the results of literature which is about physiological study, S. costatum seems to be euryhaline and eurythermal, and high affinity on the irradiance. Thus, the species might have been spread population in Jinju Bay where is characteristic of wide range of water temperature, salinity and high extinction coefficients.

• ALGAE
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• v.20 no.3
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• pp.207-216
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• 2005

Myrionecta rubra, a mixotrophic ciliate, is a cosmopolitan red tide species which is commonly found in neritic and estuarine waters. M. rubra had long been listed as an “nculturable protist”until 2 different laboratory strains were finally established in 2 research groups at the beginning of this century, enabling us to perform initiative investigation into various aspect of the live M. rubra strains (Gustafson et al. 2000; Yih et al. 2004b; Johnson and Stoecker 2005). Field sampling was carried out on high tide at 2 fixed stations around Kunsan Inner Harbor (St.1 near the Estuarine Weir and St.2 off Kunsan Ferry Station) every other day for 4 months from mid-February 2004 to understand detailed figure of the recurrent spring blooms of M. rubra following the onset of the water gates operation of the Keum River Estuarine Weir on August 1994. With its maximum abundance of 272 cells mL$^{-1}$ in St.1, fluctuation pattern of the M. rubra population at the 2 stations was strikingly similar. Notable growth of M. rubra population started on late April, to cause M. rubra red tides during one month from mid-May in which “xceptionally low salinity days”without its red tide were intermittently inserted. High abundance of M. rubra over 50 cells mL$^{-1}$ was recorded at samples with their water temperature and salinity higher than 15${^{\circ}C}$ and 4.0 psu, respectively. During pre-bloom period when salinity fluctuation is moderate and the water temperature is cooler than 15°C, Skeletonema costatum, a chain-forming centric diatom, was most dominant. Cyanobacterial species such as Aphanizomenon flos-aquae and Phormidium sp. replaced other dominant phytoplankters on the days with “xceptionally low salinity”even during the main blooming period of M. rubra. To summarize, M. rubra could form spring blooms in Keum River Estuary when the level of salinity fluctuation was more severe than that for the dominant diatom Skeletonema costatum and milder than that for the predominance by freshwater cyanobacteria. Therefore, optimal control of the scale and frequency of freshwater discharges might lead us to partially modify the fluctuation pattern of M. rubra populations as well as the period of spring blooms by M. rubra in Keum River Estuary. Sampling time interval of 2 days for the present study or daily sampling was concluded to be minimally required for the detailed exploration into the spring blooms by M. rubra populations in estuaries with weirs like Keum River Estuary.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.626-637
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• 2018

In this study, a series of survey were conducted to identify the distribution patterns of macrobenthos at the Bongam sand tidal flat in Masan Bay. We collected macrobenthos at 9 sampling sites twice in June and September of every year from 2012 to 2017 using a box core sampler (collecting area, $0.025m^2$). There was a total of 50 species with a community density of $6,388ind.m^{-2}$ and a biomass of $313.9g\;wet\;m^{-2}$ during the study period. Polychaetes had the highest number of species and density among the macrofauna, but the mollusks had the largest biomass. The number of species ranged from 10 to 25 during study period but increased to over 20 species in 2014. The density which ranged from $1,508ind.m^{-2}$ to $12,008ind.m^{-2}$ rapidly increased in 2015. The dominant species were all polychaetes such as Heteromastus filiformis, Prionospio japonicus, Hediste diadroma, and Neanthes succinea. The mean diversity index ranged from 1.2 to 1.9, richness index from 1.2 to 2.4, and evenness index from 0.5 to 0.9. From the cluster analysis results, there was a spatial difference in the similarity of faunal composition of macrobenthos and this pattern was maintained throughout the study period, that is, the temporal similarities were higher than the spatial similarities. There was a change in community composition from June 2014 to June 2015 in most of the sampling sites. During this time, the dominant species also changed from H. filiformis and N. succinea to H. filiformis and H. diadroma. The density of opportunistic species such as Capitella capitata and Polydora ligni decreased compared to the early 2000s while the population of H. diadroma increased from 2015. There was little ecological information on H. diadroma such as when and where this species occurred.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.117-124
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• 2023

The management of Ricania spp. is difficult because this pest has a wide host range and diverse habitats such as agricultural, suburban, urban, and forested areas. However, the researches for Ricania spp. management have been focused on only agricultural crops. Thus, it is required to determine the arboreal host preference of Ricania spp. at the surrounding areas of the farms to increase its management efficiency. To determine its host preference at arboreal plants, we reviewed the previous studies and investigated the densities of Ricania spp. at woody plants with high ecological importance but insufficiently studied. This study identified 120 species in 53 families of arboreal hosts of Ricania spp. Only Cornus officinalis and Styrax japonicus were preferred by all developmental stages of Ricania spp. The host preference of Ricania spp. was changed according to its developmental stages. This phenomenon would be caused by that each developmental stage of Ricania spp. would prefer different parts of woody plant, and require different nutrients for its survivor and reproduction. These results of this study could be helpful to make a plan of comprehensive management strategies for Ricania spp.

• Applied Microscopy
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• v.30 no.2
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• pp.213-232
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• 2000

The development of the knee joint was studied by electron microscopy in human fetuses ranging from 20 mm to 260 mm crown-rump length ($7\sim30$ weeks of gestational age). The appearance of the primordium of the meniscus and cruciate ligament was conspicuous as the mesenchymal cells , preceeding that of joint space at 30 mm fetus. The primitive joint cavity was first seen in the interzone from the 40 mm fetus and its intermediate layer proceeded developing as a narrow cleft which was closely incorporated with two chondrogenic layers. Poorly differentiated mesenchymal cells of the meniscus at 40 mm fetus containing predominantly free ribosomes differentiated into fibroblasts at 60 mm fetus. By 100 mm fetus, the fibroblast in inner zone of the meniscus presented as oval profiles with a short cell processes, whereas middle and peripheral zones presented as elongated cells. Differentiation of the synovial membrane coincided with clarification of the joint cavity When dilatation of the synovial cavity occurred, the two types of synovial cells were identified at 60 mm fetus. By 100 mm fetus a majority of the intimal cells were B-type. B-type cells were clearly distinguishable from A-type cells by their content of extensive rough endoplasmic reticula and well developed Golgi complexes. In contrast, A-type cells had numerous filopodia, pinocytotic vesicles, lysosomes and large vacuoles. At 260 mm fetus the B-type cells were also a majority of intimal cells. At 260 mm fetus the inner zone of the meniscus was filled with parallel oriented fascicles of collagenous fibers and oval fibroblasts. The middle zone was constituted of parallel and radially arranged fibers and fibroblasts. The outer zone was populated by elongated fibroblasts encircled by crossed collagenous fibers with the blood vessels. At 30 mm fetus the fibroblasts of the cruciate ligament contained rough endoplasmic reticula and mitochondria. Collagen fibrils were noted within narrow cytoplasmic processes which were continued with the extracellular space. Collagen fibrils of ligament were filled in the bulk of extracellular space at 100 mm fetus. By $150\sim260mm$ fetus, the cruciate ligaments were constituted of longitudinally oriented bundle of collagen fibrils with irregular rows of round cells between.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.1310-1315
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• 2004

용수수요 추정의 기본은 수도계획에 사용할 수 있는 실사용량에 대한 조사로 이에 대한 자료가 거의 없기 때문에 각 수도사업별로 제시되는 추정방법이 조금씩 상이하며, 추정방법의 진위를 가릴 수 없이 수요추정의 악순환을 초래하고 있는 실정이다. 기존 물사용량 예측은 급수량 기준의 도시 전체에 내한 평균 LPCD를 이용함에 따라 물사용 특성을 충분히 고려찬 수 없어 지역별 실제 물사용량과 큰 오차가 유발되었다. 그러므로 수도계획 및 설계에 사용할 수 있는 신뢰성 있는 설계인자를 도출하기가 불가능하여 물수요 관리정책 수립, 수도요금체계 조정 및 누수방지계획 수립 등 경제적인 수도시설의 건설에 애로를 겪고 있다. 본 연구에서는 생활용수 중 가정용수에 대하여 세탁기, 변기, 싱크대 등 수도전에 유량계를 설치 실제 가정에서 사용하고 있는 용도별 사용량을 실측, 파악하였으며, 이로부터 얻은 용도별 사용량에 대한 기간별 소비특성을 분석하였다. 이로부터 생활용수 사용량의 소비패턴 및 시간대별 부하율 산정이 가능하며, 각종 용도별 사용수량의 소비형태를 찾아낼 수 있었다. 또한 가정용수 중 용도별 물 사용비율은 세탁용수, 변기, 주방, 목욕용수의 순으로 나타났으며 주택유형별로는 아파트 연립주택, 다세대 주택, 단독주택의 순으로 단독주택에서의 물사용량이 가장 적게 나타났다. 생활용수 공급량에 내해서는 시간별, 주별, 월별 그리고 계절변동 총량을 파악하였으며 시간대별로는 오후 3시경이 최대 소비량을 보였고, 주별로는 월요일 그리고 월별로는 7월의 용수사용량이 가장 큰 것으로 조사 되었다. 본 연구로부터 도출된 용도별 실측 물사용량 자료 및 분석결과로부터, 지금까지 공급량 기준의 계획수립이 이루어져왔던 파종 수도시설 규모결정시 합리적인 용수수요예측 및 수요관리가 이루어질 수 있을 것으로 판단되며, 용수수요의 과다예측 오해 해소 등 경제적, 과학적 물관리 정책수립을 위한 기초자료를 제공할 수 있을 것으로 기대된다.는 경제적인 방법이 될 수 있다. 하천수 등의 상호 관계 분석을 통해 장기간의 유역 물순환체계 변화를 분석할 수 있었다.골풀과, 닭의장풀과가 각 1종씩으로, 조사지점( I )보다 좀 더 많은 종이 분포하는 것으로 조사되었다. 또한 어류는 조사지점( I )에서 3회에 걸쳐 총 396개체가 채집되어 3목 8과 21종이었다. 이 중 한국 고유종은 11종이었고, 외래 어종은 검정우럭과 2종이 조사되었으며, Zacco platypus(피라미), Zacco temmincki(갈겨니), Acheilongnathus koreanus(칼납자루), Odontobutis platycephala(동사리), Coreoleuciscus splendidus(쉬리) 순으로 분포하고 있었고, Acheilognathus signifer(묵납자루)는 댐 건설 전에는 많이 분포하였으나 현장조사에서 서식을 확인 할 수 언어 개체수의 큰 감소내지 멸종된 것으로 추정되었다.에서 동시에 시행하였다. 수술 후 1년 내 시행한 심초음파에서 모든 환아에서 단지 경등도 이하의 승모판 폐쇄 부전 소견을 보였다. 수술 후 조기 사망은 없었으며, 합병증으로는 유미흉이 한 명에서 있었다. 술 후 10개월째 허혈성 확장성 심근증이 호전되지 않아 Dor 술식을 시행한 후 사망한 예를 제외한 나머지 6명은 특이 증상 없이 정상 생활 중이다 결론: 좌관상동맥 페동맥이상 기시증은 드물기는 하나, 영유아기에 심근경색 및 허혈성 심근증 또는 선천성 승모판 폐쇄 부전등을 초래하는 심각한 선천성 심질환이다. 그러나 진단 즉시 직접 좌관상동맥-대동맥 이식술로 수술적 교정을 해줌으로써 좋은 성적을 기대할 수 있음을 보여주었다.특히 교사들이 중요하게 인식하는 해방적 행동에 대한 목표를 강조하여 적용할 필요가 있음을 시사하고 있다.교하여 유의한 차이가 관찰되지 않았다. 또한 HSP 환자군에서도 $IL1RN^{*}2$ allele 빈도와 carriage rat

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.79-86
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• 1974

The results from the experiment of natural collection and growth of arkshell(Anadara broughtoni, SCHRENCK) spat, which was collected at Seok-gok Bay located at Chang-weon-gun, Korea, and grown in the 1ittoral zone of Sa-gok-ri, Geo-je-gun, Korea, beginning from August 1,1973 to April 8,1974 are as follows; The larvae of arkshell came out from the very first day of the study August 1, which continued till the end of September, whose amount was not so much varied, but relatively continuous. The drifting larvae were also rather small amount, from which some 200 to 400 larvae were attached through the respective collecting equipment made from straw with the dimension of length 1.5m and breadth 12cm. It is regarded relatively large amount collected and recognised worth while enough to use as the industrial collecting method. The average shell length of spat attached was 0.54mm on 17th September, 4.11mm on 21st October, 10.47mm on 25th November in 1973 and 11.08mm on 8th April in 1974 whose progress was recognized relatively faster, comparing with the other up-to date report, The relationship between the shell length(L) and the longest radial rib(R) was as follows: Below 2.5mm of the shell length: R=0.7393 L -0.0080 Above 2.5mm of the shell length: R=0.8253 L-0.2595 And the relationship between the shell length (L) and the shell breadth (B) was as follows: Below 2.5mm of the shell length: B=0.3505 L +0.0527 Above 2.5mm of the shell length: B=0.4631 L -0.3602 The exponential curve equation between shell length (L) and the total weight (W)was as follows : Below 16mm of the shell length: W=0.19957 $L^{2.5726}$ Above 16mm of the shell length: W=0.20602 $L^{2.8400}$ In view of the above the relative growth among the shell length, the longest radial rib and the shell breadth was changed in the vicinity of 2.5mm of the shell length. And relationship between the shell length and the total weight was changed in the vicinity of 16mm of the shell length.

• Korean Journal of Ecology and Environment
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• v.51 no.4
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• pp.268-286
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• 2018

The eutrophication and algal blooms by harmful cyanobacteria (CyanoHAs) and freshwater redtide (FRT) that severely experiencing in typical regulated weir system of the Nakdong River are one of the most rapidly expanding water quality problems in Korea and worldwide. To compare with the factors of rainfall, hydrology, and dominant algae, this study explored spatiotemporal variability of the major water environmental factors by weekly intervals in eight weir pools of the Nakdong River from January 2013 to July 2017. There was a distinct difference in rainfall distribution between upstream and downstream regions. Outflow discharge using small-scale hydropower generation, overflow and fish-ways accounted for 37.4%, 60.1% and 2.5%, respectively. Excluding the flood season, the outflow was mainly due to the hydropower release through year-round. These have been associated with the drawdown of water level, water exchange rate, and the significant impact on change of dominant algae. The mean concentration (maximum value) of chlorophyll-a was $17.6mg\;m^{-3}$ ($98.2mg\;m^{-3}$) in the SAJ~GAJ and $29.6mg\;m^{-3}$ ($193.6mg\;m^{-3}$) in the DAS~HAA weir pools reaches, respectively. It has increased significantly in the downstream part where the influence of treated wastewater effluents (TWEs) is high. Indeed, very high values (>50 or $>100mg\;m^{-3}$) of chlorophyll-a concentration were observed at low flow rates and water levels. Algal assemblages that caused the blooms of CyanoHAs and FRT were the cyanobacteria Microcystis and the diatom Stephanodiscus populations, respectively. In conclusion, appropriate hydrological management practices in terms of each weir pool may need to be developed.