• Journal of Life Science
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• v.23 no.8
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• pp.1032-1040
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• 2013

The comparative effects of fibrinolytic, antioxidative activity and electrophoretical protein patterns with Cordyceps militaris powder (CM) and cordycepin-enriched Cordyceps militaris JLM0636 powder (CCM) and fermented cordycepin-enriched Cordyceps militaris JLM0636 powder by several microscopic organisms were investigated. In addition, nutritional materials such as mineral, protein, and fatty acids were also measured. The protein concentration was higher in CCM than that in CM. The protein concentration in fermented CCM was the highest in CCM treated by Aspergillus kawachii among the various samples. When total protein patterns of CM, CCM, and both fermented CCMs were analyzed by native- and SDS-PAGE, there were slightly varietal differences in electrophoretical protein patterns. Major minerals were K, Ca, Mg, and Zn. Major fatty acids were palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. Fibrinolytic activity was the highest in the fermented CCM by Bs treatment among the various samples. The ${\alpha},{\alpha}^{\prime}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) radical scavenging activity was slightly stronger in the CCM treated with Aspergillus kawachii among the various samples; however, these samples all exhibited relatively low levels of activity compared with the butylated hydroxytoluene (BHT). These results may provide the basic data necessary to understand the biological activities and chemical characteristics of Cordyceps militaris JLM0636 powder fermented by several microscopic organisms to develop functional foods.

• Journal of Conservation Science
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• v.26 no.2
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• pp.121-132
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• 2010

The Bukjiri Maaeyeoraejwasang (National Treasure No. 201) consists of two-mica granite in medium size, which was the simbol of power in the region of the Silla period. Magnetic susceptibility of the host rock was measured as 0.41(${\times}10^{-3}$ SI unit), which has the similar range with surrounding outcrop. The Buddha developed parallel discontinuous plane of NE to SW strike and damaged seriously by exfoliation, granular disintegration and brown discoloration as 41.5%, 16.7% and 40.0%, respectively. As a result of the ultrasonic velocity, which was relatively weak values as 1,629m/s (Buddha area) and 1,549m/s (surrounding outcrop), improved about 900m/s compared to last treatment. From the results of the evaluation for slope stability, identified the possibility of toppling failure in the Buddha, and planar and wedge failure in host rock. Therefore, we suggest for the safely conservation of the Buddha, continuance monitoring for understand behavior of discontinuity system of the surface, and necessitate foundation reinforcement method for the rock which has the danger of collapse.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1322-1328
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• 2006

In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect= the intestinal and immune systems and to promote their recovery from radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. In the present study, we examined the effects of HemoHIM on the maturation process of murine bone marrow (BM)-derived dendritic cells (DC). BM cells were cultured in the presence of iL-4 and GM-CSF and the generated immature DC were stimulated with HemoHIM for 24 hours. HemoHIM significantly enhanced the expression of co-stimulatory molecules, CD80 and CD86, especially. The activation capacity of HemoHIM-treated DC was significantly higher than that of immature DC, as analyzed by IL-2 and $IFN-\gamma$ production and proliferation of the responding T cells in the co-culture with allogeneic T cells. The antigen-presenting capacity of HemoHIN-treated DC was also increased by the co-culture with OVA-specific T cells (HS-1), as analyzed by IL-2 and $IFN-\gamma$ production and the proliferation. These results indicate that HemoHIM causes the maturation and ;Activation of DC, which may be a part of mechanisms of immunomodulation by HemoHIM.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.2
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• pp.133-139
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• 2018

Chemically damaged hair is vulnerable to external stimuli in daily life due to the weakened physical properties of the hair strand itself. The purpose of this work was to determine whether chemical conjugation between hair keratin proteins restores tensile strength and thus results inpreventing further deterioration under repeated combing. A model damaged hair tress was produced by a typical perm-process. Then, it was internally crosslinked by the bifunctional crosslinker (3-aminopropyl)triethoxysilane (APTES), via both silane coupling and carbodiimide chemistry. Physical properties, including tensile strength, Young's modulus, and plateau stress, were measured to verify the effect of internal crosslinking, and the existence of crosslinking was verified by Fourier transform infrared (FT-IR) spectroscopy. The degrees of hair breakage and split ends were evaluated by repeated combing-drying tests. Physical properties of chemically damaged hair were restored by internal crosslinking. Successful crosslinking of APTES via both silane coupling and carbodiimide chemistry was verified by FT-IR spectra. Prevention of breakage and split ends after repeated combing with heat was observed. Human hair can be weakened by chemical damage including perm-processing, so restoring such properties is a major issue in the hair care industry. This work shows that internal crosslinking of damaged hair via chemical conjugation would be a potent method to restore the healthy hair.

• Korean Journal of Food Science and Technology
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• v.41 no.4
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• pp.435-440
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• 2009

Acetylcholinesterase (AChE) inhibitors, which enhance cholinergic transmission by reducing the enzymatic degradation of acetylcholine, are the only source of the compound that is currently approved for the treatment of Alzheimer's disease (AD). In these experiments, the concentration samples of green tea extracts were 100, 500, and 1,000 ${\mu}g$/mL. Among them, the highest concentration (1,000 ${\mu}g$/mL) of fresh green tea extracts showed the most potent inhibitory effect on AChE by reducing more than 40% of enzyme activity, and in a concentration-dependent pattern. In addition, we examined the effect of other storing conditions on AChE inhibition. In order to maintain storage for 3 months, the materials were held at the certain storing conditions of temperature (room temperature, 4 and $-20^{\circ}C$) and for water activity (0.81, 0.69, and 0.23). In these storing conditions, the difference in temperature did not contribute to the AChE inhibitory effect. Our presented data showed that the AChE inhibitory effect was affected by the concentration of green tea extract and by water activity (0.81). These results suggest that green tea may serve as a potential dietary source of AChE inhibitor.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.475-483
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• 2019

The use of stem cells in cell-based therapy has attracted extensive interest in the field of regenerative medicine, and it has been applied to numerous incurable diseases due to the inherent abilities of self-renewal and differentiation. However, there still exist some severe obstacles, such as requirement of cell expansion before the treatment, and low survival at the treated site. To overcome these disadvantages of stem cells, we used the carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM 6) gene, which functions to increase cell-cell interaction as well as anti-apoptosis. We first confirmed whether CEACAM 6 is expressed in various cell lines at the protein level (including in stem cells), followed by evaluating and selecting the optimal transfection conditions into stem cells. The CEACAM 6 gene was transfected into stem cells to prolong cell survival and preserve from damage by oxidative stress. After confirming the CEACAM 6 expression in transfected stem cells, the cell survival was assessed under oxidative condition by exposing to hydrogen peroxide (H₂O₂) to mimic the chronic environment-induced cellular damage. CEACAM 6 expressing stem cells show increased cell viability compared to the non-CEACAM 6 expressing cells. We propose that the application of the CEACAM 6 gene is a potential option, capable of expanding and enhancing the therapeutic effects of stem cells.

• Korean journal of food and cookery science
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• v.32 no.3
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• pp.261-269
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• 2016

Purpose: The purpose of this study was to investigate the effects of mechanical processing (tumbler, tenderizer, injector) and Ganghwa mugwort extracts (GM) on the stability of chicken Neobiahni during storage for 10 days at $4^{\circ}C$. Six treatments of chicken Neobiahni were manufactured with the following conditions: CON (tumbler), CON-A (tumbler + 0.2% GM), T1(tenderizer) T1-A (tenderizer + 0.2% GM), T2 (injector), T2-A (injector + 0.2% GM). Methods: The pH, POV, TBA, and sensory characteristics of chicken Neobiahni during storage for 10 days at $4^{\circ}C$ were measured in triplicate. Results: The pH of chicken Neobiahni was in the range of 6.00-6.37, with the highest values in the treatments containing GM (CON-A, T1-A, T2-A). Mechanical processing had no significant effects during storage. The color values (lightness, redness, and yellowness) did not differ significantly in all chicken Neobiahni samples, whereas storage time had a significant effect (p<0.05). The mechanical processing combined with GM appeared to effectively control the POV and TBA levels of chicken samples during the entire storage period. In addition, sensory evaluation ratings (color, juiciness, flavor, tenderness, and overall acceptability) were improved by the mechanical processing and the addition of GM. Conclusion: These results suggest that the combination of mechanical processing and Ganghwa mugwort extracts is a useful technique for retarding lipid oxidation in chicken Neobiahni.

• Informatization Policy
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• v.26 no.3
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• pp.69-89
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• 2019

In order to implement smart cities that will become living spaces in the fourth industrial revolution era, detailed privacy information such as residents' living information, buildings and facilities information must be collected and processed in real time. While city functions and convenience for individuals are being facilitated, threats to personal information exposure and leakage are also likely to increase at the same time. Therefore, the design concept for personal information protection should be considered and accordingly reflected from the stages of smart city design, technology development and operation planning of intelligent information (AI) facilities. The results of the analysis show that for activation of smart cities and operation of data-driven cities, the concept of Privacy by Design (PbD) has already been introduced in the institutional, industrial and technological aspects, particularly in the cases of European countries and the US. In order to strengthen the local and global competitiveness of smart cities and the country, Korea also needs to actively deploy PbD as a strategy to secure a data-driven economy, which is the core strategy for smart cities. Therefore, the study suggests policy implications focused on approaches to legislative improvement and technology development support, which reflect the basic properties of PbD as defined in the study.

• Journal of the Society of Disaster Information
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• v.15 no.3
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• pp.409-417
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• 2019

Purpose: Since the implementation of the Chemical Substance Management Act, data on the number of occurrences by annual chemical accident in Korea and the contractor's contract data received from the competent authority were used. After the implementation of the contract reporting system, the contribution to the reduction of chemical accidents is summarized by statistical data. The characteristics of each region, month, type and those of similar industries and human life were compared and analyzed. Method: 4 years of chemical accident statistics from 2015 to 2018 and since 2003, we have used data from the Chemical Safety Clearing-House (CSC), which provides safety information on cases of chemical accidents. Results and Conclusion: The risk of accidents increases as a number of unskilled workers are put into the workplace during the period when the hazardous chemical handling process is temporarily suspended. Through the reporting for awarding a contract, the operators are strengthening the safety management of chemical accidents by educating unskilled workers and wearing personal protective equipment.

• The Korean Journal of Archival Studies
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• no.50
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• pp.389-423
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• 2016

This paper aims at examining the local records management under the influence of the Public Archives Act established in 2009 through a case study of prefectures and large cities with prefectural level dominions in Japan. The records management in local government has been carried out on the basis of 'the document management rule' in administrative agencies without the public archives management law. Modification of laws and ordinances in relation with records and archives management and reorganization of archives are proceeding to realize 'proper records management' in local government. Even if the content and levels are varied due to the situational conditions of local governments, the unitary records management system covering 'current and non-current' records is being adopted through the establishment of records management ordinances. In the process, local archives are pursuing strengthening and extension of their authorities and functions on records management. In addition, it is identified that observation of the provisions of records management ordinance is reinforced with public announcement of retention schedule and operation of public records management committees. These changes in local governments reflect the thought that administrative records are the intellectual property of the public and the intent of the law that accountability for the public should be achieved through the proper records management.