• The Korean Journal of Pharmacology
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• v.28 no.2
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• pp.171-179
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• 1992

Effects of a $M_1$ receptor antagonist, pirenzepine, a $M_2$ receptor antagonist, AF-DX116, and a nicotinic receptor antagonist, mecamylamine on the pressor responses to preganglionic sympathetic nerve stimulation (PNS) and McN-A-343 and DMPP in spinal (pithed) rabbits were investigated, in order to elucidate a functional role of $M_1$, $M_2$ and nicotinic receptors in ganglionic transmission. Pirenzepine and AF-DX116 selectively inhibited the McN-A-343-induced pressor response in chlorisondamine-treated rabbit and the BCh-induced bradycardia, respectively. Electrical stimulations of preganglionic sympathetic outflow at T8 level produced increases in blood pressure. Pirenzepine $(3\;{\mu}g/kg)$ significantly inhibited the PNS-induced pressor response and the degree of inhibition was not changed by increasing the doses to $100\;{\mu}g/kg$. AF-DX116 $(100\;{\mu}g/kg)$ had no effect on the PNS-induced pressor response. Mecamylamine inhibited the PNS-induced pressor response in a dose-dependent manner. The inhibitory action of mecamylamine was significantly augmented by combined-treatment with pirenzepine $(30\;{\mu}g/kg)$ but AF-DX116 $(100\;{\mu}g/kg)$ did not affect the inhibitory action of mecamylamine. McN-A-343 and DMPP elicited pressor response in the spinal rabbit. Pirenzepine and AF-DX116 dose-dependently inhibited the McN-A-343-induced pressor response but they did not affect DMPP-induced pressor response. Mecamylamine inhibited both pressor responses induced by McN-A-343 and DMPP. These results suggest that not only nicotinic receptors but also $M_1$ receptors play a facilitatory role in ganglionic transmission but $M_2$ receptors do not contribute the transmission in spinal (pithed) rabbits.

• Journal of Life Science
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• v.24 no.5
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• pp.567-572
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• 2014

Extracts of Platycodon grandiflorum have been reported to show anti-inflammatory, antioxidant, anti-metastatic, and hepato-protective effects. This study was designed to evaluate T-cell activation and M1/M2 differential macrophage activation by extracts of P. grandiflorum or P. grandiflorum containing various medicinal herbs. Using real-time RT-PCR, we analyzed expression levels of c-fos, and CD40L (T-cell activation markers) in splenocytes and iNOS, Ym1, and ARG1 in RAW 246.7 cells after treatment of CC (hot water extract of P. grandiflorum), MAEK (hot water extract of P. grandiflorum [82%] and six different plants), and HWAL (hot water extract of P. grandiflorum [7%] and eight different plants. The results showed that MAEK significantly elevated the expression of T-cell activation markers of splenocytes, with the c-fos gene activated more than 10-fold and the CD40L gene activated more than 6-fold. Although CD40L was significantly increased by CC and HWAL, the increase was only about 2-fold. In addition, CC and HWAL did not significantly activate the expression of the c-fos gene. On the other hand, CC elevated the M1 activation marker iNOS, and HWAL elevated the M2 activation marker Ym1 and ARG1 gene expression. In conclusion, MAEK could be used as an immune stimulant because of its ability to activate T cells (elicited c-fos and CD40L gene expression), whereas HWAL could serve as an anti-inflammatory agent because of its differential activation of M2 macrophages.

• Journal of Life Science
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• v.18 no.12
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• pp.1693-1699
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• 2008

Recent studies have suggested that inulin might be utilized as a prebiotics for the promotion of antimicrobial growth, but a major obstacle to the use of inulin has been its low bifidogenic effects, which were initially observed in the ceca of broiler chickens. Inulin has some problems with related to denaturation in air and lowering passage rate from upper digestive tract to caecum. To solve this problems, a newly developed compound derived by microencapsulation, inuloprebiotics, was hypothesized to enrich cecal bifidobacterial populations and reduce the colonization levels of Salmonella in the ceca of broiler chickens. The in vitro growth of intestinal beneficial bacteria including Bifidobacterium longum, Bifidobacterium bifidum, Lactobacillus acidophilus, and Lactobacillus casei grew effectively on the medium containing inulin, whereas the growth of Streptococcus aureus and Clostridium perfringens was not differences among the treatment groups. Broiler chickens consumed chow diets containing 0.5%, 0.7% or 1.0% inuloprebiotics, or a control diet without inuloprebiotics supplementation. The chickens on the inuloprebioticssupplemented diets evidenced significantly higher cecal levels of Bifidobacterium and Lactobacillus species as compared with the chickens on the control diet. The population of cecal E. coli and Salmonella was specifically reduced as the result of treatment with inuloprebiotics. However, we noted no significant differences in Bifidobacterium species, E. coli and Salmonella counts among the inuloprebiotics treatment groups. The inuloprebiotics-supplemented diets induced an increase in the serum IgG concentration. The thymus index was significantly increased in the broiler chickens that consumed diets containing 0.7% or 1.0% inuloprebiotics, with the exception of the chickens consuming the diet supplemented with 0.5% inuloprebiotics. These results indicate that the inuloprebiotic preparations exerted an immune system-promoting effect or selectively enriched the cecal Bifidobacterium species populations in the broiler chickens, and also suggest that inuloprebiotics may prove useful as a stable natural antimicrobial agent.

• Journal of Life Science
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• v.17 no.12
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• pp.1660-1668
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• 2007

The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.

• Korean Journal of Social Welfare
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• v.58 no.1
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• pp.31-58
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• 2006

The purpose of this article is to analyze the structure and characteristics of the care manager systems of the long term care insurance in Japan's social welfare institution. In order to realize such purposes, this paper develops the discussion process as follows. Firstly, this paper examines the developmental processes and contents of the long term care insurance system and the care plan services which are the services to aid the utilization of long term care services. Secondly, this paper describes the care manager which conducts the role of care plan services intentively. Thirdly, this paper makes the research framework which is formulated as the legislative views about the care manager on the long term care insurance act, task systems, recruitment and retraining systems, and research data of care manager. Summarizing the contents of care manager's circumstances and characteristics about the long term care insurance, the results are explained as follows. that is to define care manager's status as the components of care plan center, to control indirectly the activities of care manager's care plan by operating care service center and care plan center in equal corporate, to set role and task ambiguously, to recognize care manager's role as the attributed role of care service center, to give licence and qualification in local government. Therefore, it's difficult to realize care plan depending on speciality and neutrality for the service user. The essential parts for the reform of care manager systems may be to separate care service center and care plan center and to redefine the task and role of care manager, the role and task of which should be put on care plan services.

• Journal of Embryo Transfer
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• v.29 no.1
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• pp.51-57
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• 2014

The objectives of this study were to investigate the effects of trehalose as a cryoprotectant for porcine freeze-dried spermatozoa, to find the optimal freeze-drying time and storage periods of freeze-dried spermatozoa, and to find out pronuclear formation rates, cleaved rates, and embryo development through intracytoplasmic injection of freeze-dried spermatozoa on porcine oocytes. The survival rates of spermatozoa after freeze-drying with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). The highest survival rates were found at 75 mM trehalose treatment. The longer storage periods after freeze-drying seemed to have a lower survival rates. Development in culture of pig by ICSI with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). Shorter freeze-drying time of spermatozoa was resulted in the highest cleaved rates and embryo development.

• Journal of Animal Science and Technology
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• v.44 no.4
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• pp.483-490
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• 2002

The aim of this study was to determine if a low-molecular weight casein hydrolysate has an anti- hypertensive effect in spontaneously hypertensive rats (SHR). Prior to the in vivo experiment, the casein hydrolysate was confirmed to be resistant to gastrointestinal digestion by confirming the retention of its potency as an inhibitor of angiotensin I-concerting enzyme after incubation with pepsin, trypsin, or chymotrypsin. The in vivo anti-hypertensive effect of the hydrolysate was determined by the tail cuff method. Following an oral administration of the hydrolysate solution, the systolic blood pressure (SBP) decreased by 12.9% (－28.9mmHg; P<0.05) at 3 h after the administration at a dose of 500mg/kg body weight. When the hydrolysate was administered as an emulsion with 30% egg yolk, its anti-hypertensive effect was even more greater at the same dose(－30.8mmHg or －15.9%; P<0.01). In a 50-day long-term trial where the casein hydrolysate was administered once a day, the SBP-lowering effect of the hydrolysate was apparent (P<0.05) from day 35 through the end. Moreover, organ weights and plasma glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities of the administered SHR were not significantly different from those of controls at the end of the long-term trial.

• Proceedings of the Korea Technology Innovation Society Conference
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• 1999.05a
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• pp.539-563
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• 1999

이 논문은 정보통신정책 목표와의 연관성 속에서 정보통신연구개발사업의 우선순위를 설정함과 아울러 비용효과성에 바탕을 둔 적정 자원배분 도출하는데 주안점을 두고 있다. 복합적인 목표를 가진 연구개발사업에 대해 사업부문별 그리고 기술분야별로 객관적이면서도 설득력있는 자원배분지침을 마련하기 위하여 본 연구는 다기준의사결정(MCDA: Multi-criteria Decision Analysis) 방법을 채택하고 있다. 이 방법론에서는 사업의 목표와 우선순위 판단기준 및 사업대안이 계층적으로 연계된 분석구조를 설계하여 사업대안의 우선순위를 먼저 설정하고, 여기에 정수계획법(Integer Programming)을 결합하여 사업의 목표와 연계된 비용-편익 관계(Cost-benefit relationship)를 최적화하는 바탕 위에서 사업대안별 자원배분 시나리오를 제시하게 된다. 이러한 절차로 사업부문별 및 기술분야별로 각각 도출된 8개의 자원배분 시나리오는 자원배분 경향치의 안정성(Stability) 분석과 자금지원의 과부족에 대한 현실적 적합성(Relevance) 분석을 거쳐 최종적으로 하나의 적정 자원배분방안으로 수렴되도록 하였다. 우선순위 설정에 기초가 되는 사업대안의 편익에 대한 자료는 전문가의 정성적 판단을 통해 수집되었는데, 이를 위해 정보통신기술 부문에서 활동 중인 중견 이상의 총 58명의 산학연 전문가를 대상으로 한 조사를 실시하였다. 먼저, 기초기반ㆍ전략기술개발사업, 연구ㆍ기술인력양성사업, 산업응용기술개발사업, 기술기반조성사업 등 4대 사업부문에 대해서는 (ⅰ) 정보통신 기술능력의 축적, (ⅱ) 정보통신산업의 경쟁력 강화, (ⅲ) 정보통신기반의 기술고도화, (ⅳ) 정보화를 위한 기술역량의 제고 등 주요 사업의 편익을 나타내는 4개 기준으로 우선 순위를 판단하였고, 정보통신망기술, 전파 및 RF 기술, 정보처리기술, 반도체 소자/설계 기술 등 13개 기술분야에 대해서는 (ⅰ) 공공정책적 임무에 대한 기여, (ⅱ) 기술자립기반의 확충, (ⅲ) 기술발전성과 기술적 파급효과, (ⅳ) 기술적 산물의 시장잠재력 등 기술분야 연구개발을 통한 편익을 나타내는 4개 기준으로 우선순위를 판단하였다. 우선순위는 사업대안에 대한 가중치로 표시되었고, 적정 자원배분방안은 추정된 총예산 규모에 따라 지원수준에 대한 일정한 제약조건 하에서 비용-편익 관계에 따른 자원의 최적 할당량으로 제시되었다. 본 연구는 총예산 규모의 가변성, 사업 및 기술분야 대안의 분류체계에 대한 공감대 부족, 사업목표의 상호연관성과 우선순위 판단기준의 부분적 중첩성 등 여러 제약요인이 있어 도출된 구체적인 수치보다는 방향성에 더 큰 의미를 부여하는 것이 바람직하다. 그러나, 이러한 연구의 한계에도 불구하고 도출된 우선순위와 적정 자원배분방안은 문제해결의 실행타당성을 감안하여 비교적 객관적이고 균형있는 결과로 판단된다.

• Korean Journal of Poultry Science
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• v.37 no.2
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• pp.151-158
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• 2010

TCM-KWG (tissue culture medium waste after harvest of Korean wild ginseng) (panax ginseng) is left over of tissue culture medium used to grow Korean wild ginseng (KWG). The present study was conducted to investigate the effect of TCM-KWG on meat quality and the possible of application as additives in broiler chickens. A day old broiler chickens randomized in 6 groups (n=60/groups) were administered orally with 2, 4, 8, 16, 32 mL/L TCM-KWG through drinking water with one untreated control group. After administration for five weeks, we analyzed chemical composition and meat quality. Crude ash increased approximately 20% in TCM-KWG treatment groups as compared to control group whereas the concentration of moisture, crude protein and crude fat did not show any significant difference. Crude ash is essential to enhance skeleton formation and physiological function. TCM-KWG treatment gradually decreased the pH value of breast meat whereas it did not change the purge loss and cooking loss. The pH value of meat is important for preservation of meat for longer periods and high durability strength. These results suggest that TCM-KWG treatment may improve the quality of meat and can be apply as food additives in chickens.

• Microbiology and Biotechnology Letters
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• v.9 no.3
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• pp.159-164
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• 1981

Reinforced Calcium-alginate gel entrappment method for enzyme immobilization is described with an example of penicillin amidase from Bacillus megaterium KFCC 10029, a partially constitutive mutant of B. megaterium ATCC 14945. Penicillin amidase recovered from the fermentation broth by adsorption on celite is mixed with alginate and gelatin solution, and cast into a pellet or noodle form by coagulation in calcium salt solution followed by crosslinking with glutaraldehyde. Optimum pH and temperature of the immobilized enzyme preparation were 8.0 and 6$0^{\circ}C$, respectively. Kinetic constants such as Km value and the inhibition constant of 6-APA and phenylacetic acid were 2.6 mM, 7.4 mM and 21.2 mM, respectively. The enzyme leakage from the adsorbent during operation was successfully prevented owing to the increase of physical strength of gel coat. The half lives in a column reactor were 6 and 30 days at the respective temperature of 4$0^{\circ}C$ and 3$0^{\circ}C$, which were the 6-8 fold increased values as compared with those of without entrappment. The results highly recommended the use of reinforced Calcium-alginate gel entrappment method for the enhancement of physical strength and the operational stability of alginate gel entrapped enzyme.