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http://dx.doi.org/10.5187/JAST.2002.44.4.483

Antihypertensive Effects of Casein Hydrolysate in Spontaneously Hypertensive Rats  

Kim, H.S. (National Livestock Research Institute, R.D.A.)
In, Y.M. (National Livestock Research Institute, R.D.A.)
Jeong, S.G. (National Livestock Research Institute, R.D.A.)
Ham, J.S. (National Livestock Research Institute, R.D.A.)
Publication Information
Journal of Animal Science and Technology / v.44, no.4, 2002 , pp. 483-490 More about this Journal
Abstract
The aim of this study was to determine if a low-molecular weight casein hydrolysate has an anti- hypertensive effect in spontaneously hypertensive rats (SHR). Prior to the in vivo experiment, the casein hydrolysate was confirmed to be resistant to gastrointestinal digestion by confirming the retention of its potency as an inhibitor of angiotensin I-concerting enzyme after incubation with pepsin, trypsin, or chymotrypsin. The in vivo anti-hypertensive effect of the hydrolysate was determined by the tail cuff method. Following an oral administration of the hydrolysate solution, the systolic blood pressure (SBP) decreased by 12.9% (－28.9mmHg; P<0.05) at 3 h after the administration at a dose of 500mg/kg body weight. When the hydrolysate was administered as an emulsion with 30% egg yolk, its anti-hypertensive effect was even more greater at the same dose(－30.8mmHg or －15.9%; P<0.01). In a 50-day long-term trial where the casein hydrolysate was administered once a day, the SBP-lowering effect of the hydrolysate was apparent (P<0.05) from day 35 through the end. Moreover, organ weights and plasma glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities of the administered SHR were not significantly different from those of controls at the end of the long-term trial.
Keywords
Casein; Hydrolysate; Antihypertention;
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1 Hildebrand, G. E. and Tack, J. W. 2000. Micro- encapsulation of peptide and protein. International J. of Pharm. 196:173.
2 Manjusri, D. and Richard, L. S. 1975. Pulmonary angiotensin-converting enzyme. J. Biol. Chem. 250 (7):6762.
3 Maruyama, S., Mitachi, H., Awaya, J., Kurono, M., Tomizuka, N. and Suzuki, H. 1987a. Angiotensin-Ⅰ converting enzyme inhibitory acti- vity of the c-terminal hexapeptide of $\alpha_{s1}$-casein. Agric. Biol. Chem. 51(9):2557.
4 Seki, E., Osajima, K., Matsufuji, H., Matsui, T. and Osajima, Y. 1996. Resistance to gastro- intestinal proteases of the short chain peptide having reductive effect in blood pressure. Nippon Shokuhin Kagaku Kogaku Kaishi. 43(5):520.
5 Sekiya, S., Kobayashi, Y., Kita, E., Imamura, Y. and Toyama, S. 1992. Antihypertensive effects of tryptic hydrolysate of casein on normotensive and hypertensive volunteers. J. Jpn. Soc. Nutr. Food Sci. 45(6):513.
6 Shively, M. L. 1997. Multiple emulsions for the delivery of protein. In Protein delivery physical system. Plenum Press. p. 199.
7 Waynforth, H. S. 1980. Experimental and surgical technique in the rat. Academic Press. p. 212.
8 Bakhle, Y. S. 1968. Conversion of angiotensinⅠ to angiotensin Ⅱ by cell-free extracts of dog lung. Nature. 220(30):919.
9 Bernirschke, K., Garner, F. M. and Jones, T. C. 1978. Pathology of laboratory animals. Springer- Verlag, New York. USA.
10 Cushman, D. W. and Cheung, H. S. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochemical Pharmacology. 20:1637.
11 Hara, H., Funabiki, R., Iwata, M. and Yamazaki, K. I. 1984. Portal absorption of small peptides in rats under unrestrained conditions. J. Nutr. 114:1122.
12 Eto, Y., Ito, T. and Nishioka, S. 1999. Antihy- pertensive effect of alkaline protease hydrolysate of whey protein on blood pressure in spon- taneously hypertensive rats. J. Jpn. Soc. Nutr. Food Sci. 52:301.
13 Fujita, H., Sasaki, R. and Yoshikawa, M. 1995. Potentiation of the antihypertensive activity of orally administered ovokinin, a vasorelaxing peptide derived from ovalbumin, by emulsification in egg phosphatidylcholine. Biosci. Biotech. Biochem. 59(12):2344.   DOI   ScienceOn
14 Furuya, S., Sakamoto, K. and Takahashi, S. 1979. A new in vitro method for the estimation of digestibility using the intestinal fluid of the pig. Br. J. Nutr. 41:511.   DOI   ScienceOn
15 Hata, Y., Yamamoto, M., Ohni, M., Nakajima, K. and Nakamira, Y. 1996. A placebo-controlled study of the effect of sour milk on blood pressure in hypertensive subjects. Am. J. Clin. Nutr. 64:767.
16 Maruyama, S., Mitachi, H., Tanaka, H., Tomizuka, N. and Suzuki, H. 1987b. Studies on the active site and antihypertensive activity of angiotensin-Ⅰconverting enzyme inhibitors derived from casein. Agric. Biol. Chem. 51(6):1581.
17 Ondetti, M. A., Rubin, B. and Cushman, D. W. 1977. Design of specific inhibitors of angiotensin converting enzyme : New class orally active antihypertensive agents. Science. 196:441.
18 Masuda, O., Nakamura, Y. and Takano, T. 1996. Hypertensive peptide are present in aorta after oral administration of sour milk containing these peptides spontaneously hypertensive $rats^{1,2}$. J. of Nutr. 126:3063.
19 Nakamura, Y., Yamamoto, N., Sakai, K. and Takano, T. 1995. Antihypertensive effect of sour milk and peptide isolated from it that are inhibitors to angiotensinⅠ-converting enzyme. J. of Dairy Sci. 78:1253.
20 Nakamura, Y., Masuda, O. and Takamo, T. 1996. Decrease of tissue angiotensinⅠ-converting enzyme activity upon feeding sour milk in spontaneously hypertensive rats. Biosci. Biotech. and Biochem. 60(3):488.
21 Patchett, A. A., Harris, E., Tristram, E. W., Wyvratt, M. J., Wu, M. T., Taub, E., Peterson, E. R. and Ikeler, T. J. 1980. A new class of angiotensin converting enzyme inhibitor. Nature. 288(20):280.
22 Pihlanto-Leppala, A., Rokka, T. and Korhonen, H. 1998. Angiotensin-Ⅰconverting enzyme inhibitory peptides derived from bovine milk proteins. Int. Dairy J. 8:25.
23 SAS. 1996. SAS/STAT Software for PC. Releasw 6.11, SAS Institute. Cary, Nc, U.S.A.
24 Saxena, P. R. 1992. Interaction between the renin-angiotensin aldosterone and sympathetic nervous system. J. of Cardiovascular Pharma- cology. 19(6):S80.
25 Yoshi, H., Tachi, N., Sakamura, O., Takeyama, H., Ohba, R. and Itani, T. 1999. Antihypertensive effect of oligo-peptide derived from hen’s eggs, Nippon Shokuhin Kagaku Kogaku Kaishi. 46(2): 45.
26 Webster, J. and Koch, H. F. 1996. Aspects of tolerability of centrally acting antihypertensive drugs. J. of Cardiovascular Pharmacology. 27(3): S49.
27 Yamamoto, N., Akino, A. and Takano, T. 1994. Antihypertensive effect of the peptide derived from casein by on extracellular protese from Lactobacillus heveticus CP790. J. of Dairy Sci. 77:917.
28 Yokoyama, K., Chiba, H. and Yoshikawa, M. 1992. Peptide inhibitors for angiotensin-Ⅰconverting enzyme from thermolysin digest of dried bonito. Biosci. Biotech. and Biochem. 56(10):1541.
29 강경진, 김지한, 백우현. 1993. BR-900317의 in-vivo에 있어서 angiotensin 변환효소 저해작용 및 고혈압 model rat(SHR, RHR)에 있어 단회 경구투여에 의한 강압작용. 응용약물학회지. 2:220.
30 김현수, 인영민, 정석근, 함준상, 강국희, 이수원. 2002. 효소가수분해 조건에 따른 우유 케이신의 Angiotensin-Ⅰ 전환효소 저해효과. 한국축산식품학회지. 22(1):87.
31 유리나, 박수아, 정대균, 남희섭, 신재익. 1996. 대두가수분해물에서 분리한 UF-peptide가 in-vitro에서 자발성 고혈압 흰쥐의 혈압 강하에 미치는 영향. 한국식품영양학회지. 25(6):1031.
32 上野川修一. 1996. 乳の科學. 朝書店.