• Title/Summary/Keyword: 감초추출물

Search Result 178, Processing Time 0.042 seconds

Screening of Antifungal Activities of Plant Extracts against Phytopathogenic Fungi (식물추출물의 식물병원성 곰팡이 포자에 대한 발아억제 활성)

  • Park, Sang-jo;Rhu, Young Hyun;Bae, Soo Gon;Seo, Dong Hwan
    • Korean Journal of Plant Resources
    • /
    • v.30 no.4
    • /
    • pp.343-351
    • /
    • 2017
  • Plant extracts were screened for antifungal activity against major plant pathogens, Botrytis sp., Collectotrichum sp., Alternaria sp. and Cylindrocarpon sp. using 96-well microdilution method. Among the 662 methanol extracts from 401 plant species, 36 extracts showed complete inhibition of spore germination against at least one of four pathogenic fungi. Extracts of Morus alba twig and Sophora flavescens root showed minimum inhibition concentration (MIC) at $1,250{\mu}g/ml$ against Botrytis sp.. Extracts of Chloranthus japonicus root showed MIC at $1,250{\mu}g/ml$ against Collectotrichum sp.. Extracts of Glycyrrhiza uralensis aerial part, Inula helenium root and Menispermum dauricum root showed MIC between 625 and $1,250{\mu}g/ml$ against Alternaria sp.. G. uralensis aerial part and I. helenium root showed MIC at $1,250{\mu}g/ml$ against Cylindrocarpon sp.. Specifically, the extracts of Agrimonia pilosa root, Angelica tenuissima root, Asarum sieboldii root, Campsis grandifolia leaf and twig, Cnidium officinale root, Dictamnus dasycarpus root, G. uralensis aerial part, I. helenium root and M. alba twig completely inhibited spore germination at lower than $5,000{\mu}g/ml$ against all of four pathogenic fungi. Two methanol extracts from G. uralensis aerial part and M. alba twig may used as a candidate to develop into effective disease management materials in plant cultivation.

Browning inhibition of fresh-cut lotus roots by blanching in Glycyrrhiza glabra L. and Astragalus membranaceus Bunge extracts (감초, 황기 추출물로 블랜칭 처리한 신선편이 연근의 갈변 억제)

  • Kim, Han-Bit;Chung, Hun-Sik;Moon, Kwang-Deog
    • Food Science and Preservation
    • /
    • v.21 no.2
    • /
    • pp.151-156
    • /
    • 2014
  • This study was conducted to inhibit the browning of fresh-cut lotus roots by blanching in a Glycyrrhiza glabra L. and Astragalus membranaceus Bunge extract solution (0.5%). Lotus roots were cut to 1 cm using a knife. The fresh-cut lotus roots were blanched at $50^{\circ}C$ in distilled water (DW, Cont), Glycyrrhiza glabra L. extract solution (GE), and Astragalus membranaceus Bunge extract solution (AE). After treatment, they were packaged with 0.04 mm PE bags and were stored at $10^{\circ}C$ for 12 days. Their color, hardness, DPPH radical scavenging activity, pH, and total soluble solids were then investigated. During storage, the samples treated with GE and AE had high $L^*$ values. They were also found to have higher values compared to the control group in the analysis of the browning index. No difference was found, however, in the hardness analysis. With regard to the DPPH radical scavenging activity, the sample treated with Glycyrrhiza glabra L. extract had the highest activity. In the pH analysis, all the groups were found to have a decrease tendency for all the storage periods. The total soluble solids decreased in the control group and slightly increased in the AE and GE groups. These results show that blanching in Glycyrrhiza glabra L. or Astragalus membranaceus Bunge extract is effective for preventing the browning of fresh-cut lotus roots.

생약재 추출물의 수율 및 항고혈압 활성

  • 도정룡;김기주
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
    • /
    • 2003.10a
    • /
    • pp.137.2-138
    • /
    • 2003
  • 본 실험에 사용한 생약재는 금산 약초시장에서 2003년 6월에 구입하여 추출수율을 조사하였다. 그 결과, 생약재의 물 추출물의 동결건조 중량(%)이 30~40%에 달하는 생약재는 가자, 갈근, 맥문동, 산수유가 있고, 20~30%에 달하는 생약재는 감초, 구기자, 당귀, 오미자, 천궁, 황기가 이에 속했으며, 중량(%)이 10~20%에 달하는 생약재는 복분자, 오수유, 애엽, 작약, 진피, 지유, 파고지, 황련이 이에 속했다. 마지막으로 10%이하의 추출 수율을 나타낸 생약재들은 가시오가피, 강황, 계피, 석곡, 소목, 영지, 오약, 육두구, 정향, 죽엽, 필발, 초두구, 호장근이 이에 속했다. 생약재의 70% Ethanol 추출물의 동결건조중량을 살펴본 결과, 동결건조 중량(%)이 30~40%에 달하는 생약재는 가자, 당귀, 산수유가 이에 속하였고, 20~30%에 달하는 생약재는 감초, 맥문동, 구기자, 오미자, 오수유, 천궁, 황기가 속하였으며, 동결건조 중량(%)이 10~20%에 달하는 생약재는 복분자, 육두구, 애엽, 작약, 정향, 지유, 파고지, 호장근, 황련이 이에 속했다. 또한 10% 이하의 수율을 나타내는 생약재는 가시오가피, 강황, 갈근, 계피, 석곡, 소목, 영지, 오약, 익지인, 죽엽, 필발, 초두구가 이에 속하였다. 생약재의 추출 시간 및 온도에 따른 수율을 조사한 결과, 추출 시간이 경과함에 따라서 수율이 점차 증가하는 경향을 보였으며, 가자, 갈근, 황련, 복분자, 지유, 정향, 계피, 소목 순으로 높은 수율을 얻을 수 있었다. 생약재에 여러가지 효소를 이용한 가수분해 결과, 대부분의 생약재에 대해 95$^{\circ}C$에서 2시간동안 추출한 수율과 그다지 큰 차이는 보이지 않았지만 대부분의 생약재가 Termamyl 효소를 사용하여 가수분해하였을 때 가장 높은 수율을 나타내었다. 또한 지유의 경우는 Viscozyme 효소를 처리하였을 경우 가장 좋은 수율을 나타내었다. 또한, 효소의 최적 온도로 조정한 Water bath에서 0, 30, 60, 120, 210분간 효소 반응 시켜, 420nm에서 갈변도를 시간별로 측정해본 결과, Viscozyme 효소를 처리한 생약재에서 시간이 흐름에 따라 흡광도 수치의 변화가 크게 나타났다. 32종의 생약재 물 추출물의 항고혈압 활성을 측정한 결과, 파고지, 소목, 죽엽의 항고혈압 활성이 높은 것으로 나타났으며, 파고지의 효과가 가장 우수한 것으로 나타났다. 10%에탄을 추출물의 경우에는 오미자, 오수유, 소목에서 항고혈압 활성이 높은 것으로 나타났으며, 오미자의 효과가 가장 우수한 것으로 나타났다. 파고지 물 추출물을 칼럼(Sephacryl S-300, High Resolution)으로 분획하여 3개의 Peak를 얻었으며, 2번째 Peak의 항고혈압 활성이 매우 높은 것으로 나타났다.

  • PDF

Antimicrobial Activity, Quantification and Bactericidal Activities of Licorice Active Ingredients (감초 성분의 항균활성, 정량 및 방부력에 관한 연구)

  • Kim, Hye Jin;Jang, Ha Na;Bae, Jeong Yun;Ha, Ji Hoon;Park, Soo Nam
    • Microbiology and Biotechnology Letters
    • /
    • v.42 no.4
    • /
    • pp.386-392
    • /
    • 2014
  • The present study was aimed at investigating the antimicrobial activities of licorice's active ingredients. Four samples of licorice ingredients (glycyrrhizin, liquiritin, liquiritigenin, and isoliquiritigenin) were evaluated for their antimicrobial activities against six skin microorganisms. The bioassay applied for determining the antimicrobial effects employed a disc diffusion assay, the minimum inhibitory concentration, and the challenge test. The ingredients showed antibacterial activities. Especially, isoliquiritigenin has significant antimicrobial activities against two Gram-positive (Bacillus subtilis, Propionobacterium acnes) and two Gramnegative (Escherichia coli, Pseudomonas aeruginosa) bacteria. These samples had much higher antimicrobial activities than synthetic preservatives. Our results reveal that liquiritigenin and isoliquiritigenin could be useful compounds for the development of antibacterial agents for the preservation of cosmetics and foods. The two flavonoids, liquiritigenin and isoliquiritigenin, sourced from Korea, China, Uzbekistan, were quantified using HPLC. The results demonstrated that Korean licorice has two flavonoids (liquiritigenin, isoliquiritigenin) in much higher quantities than was observed in the licorice obtained from the two other countries. Thus, isoliquiritigenin and Korean licorice extract represent new candidates for antimicrobial agents.

In vitro screening of the acetylcholinesterase inhibition, antioxidant activity, and neuronal cell protective effect of medicinal plant extracts (생약추출물의 acetylcholinesterase 저해, 항산화 및 신경세포보호 효과 in vitro 탐색)

  • Um, Min Young;Ha, Tae Youl;Seong, Ki Seung;Kim, Yong Sik
    • Food Science and Preservation
    • /
    • v.20 no.6
    • /
    • pp.840-845
    • /
    • 2013
  • This study investigated the effects (i.e., the acetylcholinesterase activity, lipid peroxidation, and neuronal survival) of 20 kinds of medicinal water extracts. The water extracts of three medicinal plants - Cornus officinalis, Glycyrrhiza glabra, and Angelica gigas - were found to be the most effective on acetylcholinesterase inhibitory activity. In the lipid peroxidation-generating system induced by $H_2O_2/FeSO_4$ in rat brain homogenates, Perilla frutescens, Polygonum multiflorum, Cinnamomun cassia, and G. glabra exhibited protective activity against lipid peroxidation. The neuronal cell death induced by L-glutamate in PC12 was suppressed by the water extracts of G. glabra, Cinnamomun cassia, Platycodon grandiflorum, and Mentha arvensis at the concentration of $100{\mu}g/mL$. Taken together, these results showed that the water extract of G. glabra has the potential anti-dementia activity, which suggests that it might provide an effective strategy for improving dementia.

Effect of Improvement of Storage Properties and Reducing of Sodium Nitrate by Glycyrrhiza uralensis and Curcula longa in Pork Sausage (돈육 소시지에 첨가한 감초 및 강황의 저장성 증진 및 아질산염 소거 효과)

  • Cho, Sun-Hee;Jung, Soon-Ah;Song, Eu-Jin;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Park, Jin-Gyu;Park, Sun-Mi;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.35 no.8
    • /
    • pp.997-1004
    • /
    • 2006
  • This study was performed to investigate the storage properties of emulsion-type sausage added with Glycyrrhiza uralensis and Curcula longa hot water extracts (GCHE) extracted at $10^{\circ}C$ for 35 days. The TBARS values of sausages containing 1.0 and 5.0% GCHE were lower than that of control. The bacterial count and VBN contents of all the samples were significantly increased during the storage periods, except the group with 5.0% GCHE. The redness of all the samples was lower than that of control, while yellowness of all the samples was higher than that of control. The nitrite scavenging abilities of the sausages containing 1.0 and 5.0% GCHE were higher than that of the control. It is suggested that the addition of 1.0% hot water extracts extracted from these plants may be a potential substitute for the use of nitrite to extend shelf-life of sausages.

Fermentative Properties and Immunomodulating Activity of Low-sodium Kimchi Supplemented with Acanthopanax senticosus and Glycyrrhizae uralensis Extracts (가시오가피와 감초 추출물을 첨가한 저 나트륨 김치의 발효특성과 면역 활성능)

  • Yu, Kwang-Won;Suh, Hyung-Joo;Hwang, Jong-Hyun
    • The Korean Journal of Food And Nutrition
    • /
    • v.25 no.4
    • /
    • pp.878-887
    • /
    • 2012
  • This study investigated the fermentative characteristics and immunomodulating activity in Kimchi added with various salts (salt replacement and herb-salt with Acanthopanax senticosus and Glycyrrhizae uralensis) for the reduction of Na concentration in Kimchi. Kimchi using a salt replacement and herb-salt showed a higher level of acidity (0.8~0.84%) than that of the control (0.7%) at 7-day fermentation. Kimchi using a salt replacement and herb-salt showed a lower level of salinity (1.72~1.98%) than that of control (2.3~2.57%) during fermentation. The growth of Lactobacillus spp. and Leuconostoc spp. recorded the highest level ($2.3{\times}10^8$ and $2.8{\times}10^6cfu/g$, respectively) in control at 6 day-fermentation. However, those levels in Kimchi prepared with salt replacement and herb-salt were $3.5{\sim}5.4{\times}10^8$ and $6.1{\times}10^6cfu/g$, respectively. It is assumed that the high level of acidity of Kimchi prepared with salt replacement and herb-salt was caused by the increase in the growth of Lactobacillus spp. and Leuconostoc spp.. When the macrophage stimulating activity of salt replacement kimchi (Salt-R kimchi) supplemented with hot-water extract from Acanthopanax sentisus (AS) or Glycyrrhiza uralensis (GU) was investigated on aging period, Salt-RA kimchi with AS 5% at 6 days (2.78-fold of saline control at $100{\mu}g/m{\ell}$) and Salt-RG kimchi with GU 5% at 9 days (2.02-fold) significantly increased compared to the Salt-RA kimchi without AS or GU. In addition, Salt-RAG kimchi with AS 3% and GU 3% improved the bitter taste of Salt-RA and potently stimulated the macrophage at 6 days (1.28-fold of Salt-R kimchi) even though its activity was lower than Salt-RA (5%, 1.39-fold).

Growth Characteristics of Soybean Sprouts Cultivated with Extract of Korean Herb Medicines (국산 한약재 추출물로 재배한 콩나물의 생육특성)

  • 최상도;김윤희;남상해;손미예
    • Food Science and Preservation
    • /
    • v.9 no.2
    • /
    • pp.168-173
    • /
    • 2002
  • Growth characteristics and crude protein content of soybean sprouts cultivated with extracts of some Korean herb medicines for 4 days were investigated. Extracts of Korean herb medicines were utilized and adjusted to 100 ppm, 200 ppm, 300 ppm and 400 ppm as culturing water of soybean sprouts. Of Korean herb medicines tested, extract of Panax ginseng was most effective to increase of weight and length of soybean sprouts, followed by Glycyrrhiza glabra. Extracts of Angelica gigas and Cnidium officinale were similar with that of control group. Extract of Coptidis japonica inhibited the growth of soybean sprout. Optimal concentrations of extracts of Glycyrrhiza glabra and Panax ginseng for growth of soybean sprout were 100 ppm and 200 ppm, respectively. Weight of soybean sprout cultivated with extract of Panax ginseng (PGE) was increased to 7.2%(wet basis) as compared with that of control group. Length of soybean sprouts cultivated with 200 ppm of PGE was accelerated to 32% and increased to 50% in soybean sprout cultivated for 3 days. Crude protein contents of soybean sprout cultivated with extract of Angelica gigas or Panax ginseng was slightly increased according to cultivation period. In Cnidium officinale group, crude protein contents was increased at a 300∼400 ppm concentration and 4 days of cultivation.