• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.494-498
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• 1989

Hybridoma cell lines, which secrete monoclonal antibodies (mAbs) against the surface antigens of Cryptosporidium parvum Sporozoites, were produced by fusing spleen cells of C. parvum Sporozoite-immunized mice with P3-X63-Ag8 myeloma cells. Two cloned antibody-secreting cell lines, Kor1 and Ea2, were established and produced IgG1 and IgG2a antibodies, respectively. Percoll-purified sporozoites were solubilized and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Western blot assay demonstrates that an antigen of 20-kDa was bound by monoclonals. By indirect immunofluorescence microscopy, mAb exhibited uniform binding to the sporozoite surface.

• Korean Journal of Veterinary Research
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• v.6 no.1
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• pp.1-9
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• 1966

From thse studies, the methodology of the complement fixation test for hog cholera has been described, and the application of the test for the diagnosis of hog cholera has been suggested.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2002.05a
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• pp.21-29
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• 2002

With regard to the personnel safety and other safety when the gas suppressants are discharged into the area where occupants exist, the short term and long term effects to the health of people are discussed mainly with the Carbon dioxide agent and Halon Replacement agents system. To gain the benefits of CO2 extinguishing systems while minimizing risk to people serious attention must be given to personnel safety in the design, installation, and maintenance of CO2 systems. Training of personnel is essential. A major factor in the use of a clean agent fire suppressant in a normally occupied area is toxicity. While all halocarbon agents are tested for long-term health hazards, the primary endpoint is acute or short-term exposure, The primary acute toxicity effects of the halocarbon agents described here are anesthesia and cardiac sensitization. For inert gases, the primary physiological concern is reduced oxygen concentration.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.35C no.1
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• pp.1-9
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• 1998

In the circuit model that outputs are latched and input vectors are successively applied at inputs, the gate resizing approach to reduce the delay of the critical pathe may not improve the performance. Since the clock period is etermined by delays of both long and short paths in combinational circuits, the performance (clock period) can be optimized by decreasing the delay of the longest path, or increasing the delay of the shortest path. In order to achieve the desired clock period of a circuit, gates lying in sensitizable long and short paths can be selected for resizing. However, the gate selection in path sensitization approach is a difficult problem due to the fact that resizing a gate in shortest path may change the longest sensitizable path and viceversa. For feasible settings of the clock period, new algorithms and corresponding gate selection methods for resizing are proposed in this paper. Our new gate selection methods prevent the delay of the longest path from increasing while resizing a gate in the shortest path and prevent the delay of the shortest path from decreasing while resizing a gate in the longest sensitizable path. As a result, each resizing step is guaranteed not to increase the clock period. Our algorithmsare teted on ISCAS85 benchmark circuits and experimental results show that the clock period can beoptimized efficiently with out gate selection methods.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.344-351
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• 1984

Crude 3 M KCI extracts of ovine foetal skin (FLS) were shown to induce serum inhibition of lymphocyte blastogenesis when injected to normal sheep. When fractionated through Sephadex C-150 columns, four protein peaks were obtained, immunosuppressive activity in vitro and in vivo being associated with Peak IV. In discussion, the immunosuppressive activity of FLS is compared with that obtained with extracts of ovine squamous cell carcinoma, and possible identity of the immunosuppressors is suggested.

• Toxicological Research
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• v.19 no.4
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• pp.285-291
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• 2003

The use of skin whitening agents has been recently increased in various kinds of cosmetic products, although there were some reports that whitening agents might cause allergic contact dermatitis. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pigs for contact sensitization potential. This study was carried out to investigate the skin sensitization potential of three whitening agents, arbutin, azelaic acid, and kojic acid, by LLNA using a non-radiois-topic endpoint. Female Balb/c mice were exposed topically to a weak allergen, $\alpha$-hexylcinnamalde-hyde (HCA), and three whitening agents following LLNA protocol. Lymph node (LN) weight and cell proliferation in ears and auricular lymph node using bromodeoxyuridine (BrdU) immunohistochemistry were evaluated. LN weights were significantly increased at the HCA group compared to the vehicle control. A weak allergen, HCA elicited 3-fold or greater increase in cell proliferation of lymph nodes as well as increase in cell proliferation of ear as measured by BrdU immunohistochemistry. However, in the case of skin whitening agent groups, there were no significant changes in LN weight and cell proliferation in the ear and lymph node of mice treated with 5, 10 and 20% of three whitening agents compared to the vehicle control. These results show that these three skin whitening agents may not have contact sensitization potentials at tested concentrations in Balb/c mice by LLNA.

• Proceedings of the KAIS Fall Conference
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• 2008.11a
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• pp.463-464
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• 2008

한국산 약용광물로부터 얻은 미네랄 혼합물이 실험쥐의 알레르기성 염증에 미치는 효과를 살펴보기 위하여 혈청 IgE 농도, 염증성 cytokine 농도, 히스타민 방출정도를 측정하였다. 알부민으로 알레르기성 염증을 유도한 실험쥐에 광물성 미네랄을 처리한 결과 혈청 IgE의 농도가 현저히 감소하였다. 또한 실험쥐 기관지 폐포 세척액의 염증성 cytokine의 농도도 크게 감소하였다. 알부민으로 알레르기성 염증을 유도한 쥐의 폐 조직내 염증세포의 침윤을 확인한 결과 광물성 미네랄을 처리한 쥐의 폐 조직에서는 염증 세포수가 거의 정상군 수준으로 감소하였다. 쥐의 호염기성 백혈병 세포에 anti-DNP-IgE를 감작시킨 후 광물성 미네랄을 처리하고 히스타민 방출양을 측정한 결과 히스타민 방출이 크게 감소하였다. 이러한 결과는 한국산 약용광물의 미네랄이 실험쥐의 알레르기 염증을 억제하는 효과가 있음을 보여준다.

• Research in Plant Disease
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• v.7 no.3
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• pp.170-174
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• 2001

Gelatin particle agglutination test (GPAT) was optimized for detection of Tobamovirus and contamination of the virus in commercial pepper seeds was evaluated. The optimum concentration of ${\gamma}$-globulin G, specific to tobacco mosaic virus pepper strain, was 100 ug/ml. The sensitivity of GPAT for the detection of Tobamovirus in pepper seeds was as high as enzyme-linked immunosorbent and dot immunoblotting assays. Optimum dilution ranges of the seed extract for GPAT was 5-25 folds. Using the optimized GPAT with above conditions, the rate of Tobamovirus contamination in seeds was turned out to be average of 79.1%.

• Journal of Dental Rehabilitation and Applied Science
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• v.28 no.2
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• pp.171-178
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• 2012

Botulinum toxin type A (BoNT-A) has been applied successfully to treat chronic migraine, dystonia, spasticity and temporomandubular disorders(TMDs) as well as frontal wrinkle and glabella wrinkle. Recently it has been reported that BoNT-A, reversibly blocks presynaptic acetylcholine release, also inhibits the release of substance P, CGRP(calcitonin gene related peptide) and glutamate related to peripheral sensitization and neurogenic inflammation in sensory nerve, In this study we reviewed animal nerve injury model such as rat and rabbit and identify the analgesic effect and mechanism of nerve injury pain after dental treatment.

• YAKHAK HOEJI
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• v.41 no.1
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• pp.139-146
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• 1997

In the present study, capsaicin-induced desensitization of peripheral sensory nerves were investigated by using guinea pig bronchi, in which these nerves are stimulated with cap saicin to produce a contractile response via the release of sensory neuropeptides such as substance P and neurokinin A. The contractile response to capsaicin was inhibited by the combination of CP96345 and SR 48968 suggesting that the excitatory effect of capsaicin is mediated via both the tachykinin NK-1 and NK-2 receptor. Capsaicin produced in vitro-desensitization in dose-dependent manner, but after this in vitro-desensitization the response to NK-1 and NK-2 receptor agonist did not change. Systemic administration (s.c.) of capsaicin also desensitized significantly bronchial tissues but could not produce any change in the contractile response to the selective agonists of NK-1 and NK-2 receptor. Therefore, the present results suggest that functional desensitization to capsaicin-induced contractile response in guinea pig bronchi does not involve NK-1 and NK-2 receptor, while excitatory effect of capsaicin is mediated via both NK-1 and NK-2 receptor. In conclusion, it is suggested that capsaicin- induced excitation and desensitization involves somewhat different pathways.