• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.63-68
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• 2013

ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.

• Journal of Korea Foresty Energy
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• v.17 no.1
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• pp.56-70
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• 1998

This study was carried out to investigate the structural characteristics of kraft lignin and the wood degrading characteristics, the productivity of ligninolytic enzymes and the enzymatic degradation of kraft lignin by white-rot fungi. To purify kraft lignin, precipitation of kraft pulping black liquors of pitch pine meal was done by titration with lN $H_{2}SO_{4}$ reaching to pH 2, and isolation of the precipitates done by centrifugation. The isolated precipitates from pitch pine were redissloved in lN NaOH, reprecipitated by titration with lN $H_{2}SO_{4}$, washed with deionized water, and kept ofr analysis after freeze drying. Fractionation of the precipitates in solution by successive extraction with $CH_{2}Cl_{2}$ and MeOH, and the fractionates were named SwKL, SwKL I, SwKL II, and SwKL III for pitch pine kraft lignin. The more molecular weights of kraft lignin increased, the less phenolic hydroxyl groups and the more aliphatic hydroxyl groups. Because as the molecular weights increased, the ratio of etherified guaiayl/syringyl(G/S ratio) and the percentage were increased. The spectra obtained by 13C NMR and FTIR assigned by comparing the chemical shifts of various signals with shifts of signals from autherized ones reported. The optimal growth temperature and pH of white-rot fungi in medium were $28^{\circ}C$ and 4.5-5.0, respectively. Especially, in temperature and pH range, and mycelial growth, the best white-rot fungus selected was Phanerochaete chrysosporium for biodegradation. For the degradation pathways, the ligninolytic fungus jcultivated with stationary culture using medium of 1% kraft lignin as a substrate for 3 weeks at $28^{\circ}C$. The weight loss of pitch pine kraft lignin was 15.8%. The degraded products extracted successively methoanol, 90% dioxane and diethyl ether. The ether solubles were analyzed by HPLC. Kraft lignin degradation was initiated in $\beta$-O-4 bonds of lignin by the laccase from Phanerochaete chrysosporium and the degraded compounds were produced from the cleavage of $C\alpha$-$C\beta$ linkages at the side chains by oxidation process. After $C\alpha$-$C\beta$ cleavage, $C\alpha$-Carbon was oxidized and changed into aldehyde and acidic compounds such as syringic acid, syringic aldehyde and vanilline. And the other compound as quinonemethide, coumarin, was analyzed. The structural characteristics of kraft lignin were composed of guaiacyl group substituted functional OHs, methoxyl, and carbonyl at C-3, -4, and -5 and these groups were combinated with $\alpha$ aryl ether, $\beta$ aryl ether and biphenyl. Kraft lignin degradation pathways by Phanerochaete chrysosporium were initially accomplished cleavage of $C\alpha$-$C\beta$ linkages and $C\alpha$ oxidation at the propyl side chains and finally cleavage of aromatic ring and oxidation of OHs.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.22-33
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• 2002

This study was conducted to investigate the effects of fragmentation on the bird community in Seongduck-ri and Hanggum-ri agricultural landscapes of Kangha-myon, Yangpyong-gun, Kyonggido. We analyzed the composition of tree species, forest environmental structures, structures of agricultural landscapes, avifaunas and guild structures. Number and density of tree species ranging from 6 to 10cm in D.B.H. were high in Seongduck-ri, but basal area was high in Hanggum-ri. Coverages of foliage layers under two meters and over 12 meters were high in Hanggum-ri. Meanwhile, Seongduck-ri agricultural landscapes consisted of twelve patches, dominated by Larix leptolepsis and Robinia pseudoacacia Hanggum-ri landscapes was composed of eleven patches, and dominated by Pinus rigida, Castanea crenate, Larix leptolepsis and paddy field. Dominant bird species showed the same order in two study areas, thirty-six species of birds were observed in Hanggum-ri landscapes, but twenty species were in Seongduck-ri landscapes. Number of species and density belonging to hole-, bush-, ground-nesting and water-, bush-, ground-foraging guild were high in Hanggum-ri landscapes. Number of species and density belonging to hole-nesting guild showed the high value in Hanggum-ri landscapes that was not fragmented. It could be attributed to the low human disturbance and relative high density of medium and large trees in D.B.H. Also, number of species and density belonging to bush-, ground- and water- foraging guild showed the high value in Hanggum-ri landscapes that had the high foliage amounts in the foliage layer under two meters. It will be related with the paddy field, dry field and brook, which patches covered the 28.4% of Hanggum-ri landscapes. Therefore, road not only dominate the heterogeneous patch in agricultural landscapes, but also easily cause the human disturbance. Thus, road construction would decrease the natural patch diversity and edge effects that are important for sustaining the diverse avifauna in agricultural landscapes.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.62-70
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• 2014

This study was carried out to analysis the vegetation status, the habitat size and the meaning of new natural habitat, and to provide basic data for conservation or management on the newly augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. A. distichum is a Korean monotypic endemic species. Most natural habitats of A. distichum have been reported in South-Central area around Chungcheong-do. The extent of occurrence of A. distichum can be enlarged to Central area due to the Yeoju-si habitat. The new habitat is located on a hillock which is composed of rock field at $37^{\circ}20{\sim}21^{\prime}N$, $137^{\circ}43{\sim}21^{\prime}E$, and 99~120m a.s.l.. The habitat size is small as about $530m^2$, and a small stream runs aside. The number of individuals of A. distichum was about 1,200. But most individuals were smaller than 0.5m height, so just about 300 individuals are taller than 1.0m height. A. distichum on Yeoju-si seemed to be maintained and distributed by vegetative propagation from elongated roots of branches. Pinus rigida and Quercus aliena dominated tree layer, and Lindera obtusiloba, Q. aliena and Acer tatricum appeared frequently in subtree layer. The shrub layer was dominated by A. distichum with Stephanandra incisa, Euonymus alatus for. ciliatodentatus, Ligustrum obtusifolium, etc. The coverage of herbaceous layer was low.

• Korean Journal of Environment and Ecology
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• v.30 no.3
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• pp.386-398
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• 2016

To understand the vegetation structure in the ridgeline area of Nakdong-jeongmaek, six primary areas in Nakdong-jeongmaek were selected and their vegetation distribution was surveyed considering the environmental conditions and artificial influences. According to the results of community classification based on TWINSPAN, the vegetation in the surveyed region was categorized into 9 groups: Betula costata-Quercus mongolica community, Q. mongolica community, Pinus densiflora-Q. mongolica community, P. densiflora community, Deciduous oaks-P. densiflora community, Deciduous oaks community, P. thunbergii community, P. koraiensis-P. rigida community, and Chamaecyparis obtusa-Alnus firma community. In Baekbyeongsan(Mt.) located in Taebaek-si of Gangwon-do, Betula costata-Quercus mongolica community was found, reflecting the environmental characteristics of northern temperate climate. P. thunbergii community appeared in Gudeoksan(Mt.) of Busan metropolitan city, which is near the coast. Since Gudeoksan(Mt.) is near to the downtown and its altitude above the sea is relatively low, people visit the area often. Therefore, C. obtusa and P. thunbergii have been planted for producing forest trees and implementing anti-erosion afforestation. In the other primary survey areas, Q. mongolica-dominant communities, P. densiflora-dominant communities, and deciduous oak-dominant communities, which are representative forest vegetation types of Jeongmaeks in South Korea, were mainly distributed, showing no significant difference compared to the forest vegetation types of other Jeongmaeks. Since the Nakdong-jeongmaek from south to north, it shows clear characteristics of vegetation changes between the northern temperate climate and the warm temperate climate of the south.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.1
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• pp.109-116
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• 2002

This study was performed to investigate the effect of kimchi intake on antiaging characteristics in liver of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzyme activities. Two hundred twenty SAM were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK) group, mustard leaf added (30%) Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Amount of freez-dried kimchi added to the diet was 5% that is equivalent to 50 g of fresh kimchi. Concentrations of total free radical, OH radical, $H_2O$$_2$in the liver significantly increased as aged (p<0.05). But those free radical concentrations from kimchi diet groups were lower than those of control (p<0.05). Among kimchi groups, MKCK and MLK groues showed greater inhibiting effect than KCK. Antioxidant enzyme activities of Cu, Zn-SOD, Mn-SOD, GSH-px, catalase and GSH/GSSG in kimchi groups were significantly increased (P

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.1
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• pp.117-123
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• 2002

This study was Performed to investigate the effect of kimchi intake on antiaging characteristics in the brain of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzymes. Two hundreds twenty SAM (20 mice) were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK)group, 30% mustard leaf added Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Concentrations of total free radical, OH radical and $H_2O_2$ of control group increased up to 123%, 262% and 174% of initial value (p<0.05) after one year. Increase in free radical production in kimchi groups due to aging was decreased by kimchi feeding. Among kimchi groups. MKCK and MLK groups showed greater inhibiting effect against free radical production than KCK. The concentration of TBARS in the bruin of control group also significantly increased up to 362% of initial value as aged (p<0.05) and production of TBARS in kimchi groups were decreased. When the activities of Cu, Zn-SOD, Mn-SOD, GSH-px and catalase of kimchi groups were compared to those of control at the same experimental period, anti-oxidative enzyme activities of kimchi groups were lower than those of control (p<0.05). But GSH/GSSG in kimchi groups were higher compared to control. In conclusion, decrease in free radical production and increase in anti-oxidative enzyme activities were observed from kimchi groups suggesting that kimchi might have important role on retarding aging. Among kimchi variety tested in this experiment, MKCK and MLK seem to have greater effect on inhibiting free radical production and increasing anti-oxidative enzyme activities than KCK.

• Journal of Korean Society of Forest Science
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• v.103 no.3
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• pp.510-518
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• 2014

Conflicts between humans and wild boars (Sus scrofa) have increased because of causing crop damage and appearing in downtown. Management to reduce population size has mostly been implemented on pests, but knowledges about ecological values are very rare. Rubbing trees by wild boar was investigated to figure out characteristics and for management ecologically from July to October 2013 in a mixed forest, Geochang, Gyeongnam Province, Korea. Pinus rigida was most frequently founded, and rubbing trees, both conifers and deciduous, were mainly located in 200~600 m above sea level. DBH was no significant difference between rubbing and control trees. The use intensity of conifers was higher than that of deciduous. The coverage of foliage layers and the number of woody plants within a radius of 2.5 m of trees (rubbing and control) were also investigated and compared to determine ecological characteristics. There were 1,332 individuals founded from 25 families. In the coverage of foliage layers, the understory and midstory showed statistically significant, but not in the subover and overstory. The number of Shrubs and saplings are higher in rubbing trees than those of control, but no difference in stems. Total individuals, the number of species, species diversity index(H') within a radius 2.5 m is considerably higher in rubbing trees with high intensity than those of low intensity and control. Our results show that wild boar mainly selected and used conifers more frequently as rubbing trees. Wild boar also modified the physical environments around rubbing trees. Consequently, the positive effects such as species diversity is increase. Thus the conservation policies is required after the intensive population reduction every 3-4 years for forest ecological management rather than annual sustainable hunting.

• Journal of the Korean Wood Science and Technology
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• v.45 no.3
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• pp.297-307
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• 2017

This research was performed to use recycling wood fiber from waste MDF as raw materials for manufacturing of interior decorative accessories. Virgin fiber of Pinus rigida for manufacturing MDF and recycling fiber from manufactured MDF with virgin fiber were dyed by using reactive dyes (Bis-monochlorotriazine and Vinyl sulfone type), vat dyes (Anthraquinone type), direct dyes (Diazo type) such as red, yellow and blue, and natural dyes using gardenia or sappan wood, and they were examined to evaluate their dyeing properties and sunlight fastness. The hue of virgin fiber and recycling fiber were 4.2YR, and 4.4YR, respectively, which showed red-yellowish color. The recycling fiber looked a little darker than the virgin fiber, where $L^*$ values of the recycling fiber showed a little lower. Reactive, vat and direct dyes dyed well both the virgin and recycling fibers. The recycling fiber showed a little higher values of colour yield and a little lower in $L^*$, but it seemed that there was no significant difference. The Hue values of the recycling fiber and the virgin fiber dyed with sappan wood were 4.4YR and 4.0YR, showing no difference between/after dyeing. However the Hue values of the recycling fiber and the virgin fiber dyed with gardenia were 7.4YR and 6.9YR, respectively. Those values were much higher than the values of the fibers dyed with other chemical dyes. But the fibers dyed with gardenia showed poor sunlight fastness.

• Journal of the Korean Wood Science and Technology
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• v.13 no.3
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• pp.41-48
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• 1985

In near future about 500,000 hectares of Japanese larch and pitch pine need thinning to improve crop-tree spacing and reasonable species composition in Korea. Although thinned trees show very little high quality wood, in fact, all the sawed products are suitable for construction and farming materials. Studies on the utilization of thinned small-diameter trees have been implemented in order to increase lumber yield and display the skeleton of farm buildings. The results obtained in this study were summarized as follows: 1. According to Korean Conifers Log Grading System, log grade 3 was 87.4 percent in Japanese larch bolts and 95.8 percent in pitch pine bolts from thinned trees. The ability to identify defects was essential for grading logs. 2. Cutting diagrams of sawing were developed for 1-sided sawing, 2-sided sawing and 3-sided sawing by small end diameter class of bolt and cutting sizes also were found to use effectively lumber with wane. 3. Lumber recovery of Pitch pine was a little higher than that of Japanese larch. The maximum lumber yields of pitch pine by sawing patterns were 87.2 percent for 1-sided sawing, 80.8 percent for 2-sided sawing an 64.1 percent for 3-sided sawing, up yield compared with 58.1 percent for 4-sided sawing. The lumber yield of 1, 2 and 3-sided sawing significantly was higher value than that of 4-sided sawing. 4. Lumber was inspected and graded. Lumber quality of diameter class 12cm was a little better than that of diameter class 8cm, but 90 percent of lumber in diameter c1ass 16cm fell into grade 3. The lumber grade was classified by Korean standard grades of confiers lumber. 5. Lumber from 1, 2 and 3-sided sawing was used to build the skeleton of a farm house ($53m^2$) and greenhouse ($60m^2$) in order to display more efficient use of small diameter trees from thinning 6. Total volume of timber for building a farm house was $8.1m^3$ of imported timber. Timber of $7m^3$, 70 percent of total volume would be substituted imported timber for domestic timber.