• Bulletin of the Korean Chemical Society
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• 제29권8호
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• pp.1627-1629
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• 2008

• 한국인터넷방송통신학회논문지
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• 제23권4호
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• pp.59-64
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• 2023

본 논문은 전자기기에 사용되는 전원의 노이즈 저감을 위한 전도성(Conducted) EMI 필터의 경험적 설계를 통하여 제안하였다. 제안된 구조는 A형, B형, C형, D형의 구조를 설계하였으며, 다양한 X-커패스터와, Y-커패스터, Air-인덕터로 LC 네트워크 사용으로 전도성 노이즈 저감을 확인하였다. L1, L2는 10[μH]를 사용하였으며, C1, C2는 4.7[nF]를 사용하였다. 공통모드(Common Mode)용 L3는 13[μH]를 사용하였으며, C5, C6, C7은 10[nF]을 사용하여 설계하였다. 설계된 EMI 필터의 측정된 삽입손실 값은 3.2MHz에서 -74.4[dB], 4MHz에서 -75.4[dB], 13.56MHz에서 -75.3[dB]로 나타났다. 따라서 제안한 EMI 필터는 다양한 전자기기에 사용되는 전원 노이즈 저감에 이용될 것이다.

• 생명과학회지
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• 제26권2호
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• pp.181-189
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• 2016

본 연구에서는 발아전처리 제초제인 Dithiopyr (DTP)를 시험물질로 사용하여 급성 독성시험을 평가하고, zebrafish의 행동에 미치는 영향을 연구하였다. 급성 독성시험 결과 zebrafish에서의 DTP의 LC₅₀값은 14.49 μM이었다. 또한 DTP 노출이 유영 패턴에 미치는 영향을 관찰하기 위하여 아치사농도(2.5-20 μM)에서 novel tank test (NTT)와 open field test (OFT)의 두 가지 행동실험을 실시하였다. NTT를 실시한 결과, 하층부의 머무는 시간 및 최초로 상층부에 들어간 시간, 회전각, 부동 시간의 경우 control 그룹과 비교하여 통계적으로 유의성 있게 농도 의존적으로 증가하였다(p<0.05). 또한 상층부에 진입한 횟수 및 평균 유영속도에서 통계적인 유의성 있는 감소현상이 확인되었다(p<0.05). OFT를 실시한 결과, 총 이동 거리 및 평균 유영속도에서 유의성이 있는 감소현상이 확인되었다(p<0.05). 부동 시간 및 회전각, 굴곡유영에서는 유의성 있는 증가 현상이 확인되었다(p<0.05). 또한 Whole-body cortisol level 측정 결과, 저농도 노출 그룹에서만 유의성 있는 증가가 관찰되었으나(p<0.05), 5와 10 μM에서는 유의성 있는 차이가 관찰되지 않아 농도의존적으로 감소하는 것을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제20권5호
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• pp.614-618
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• 1999

• 한국축산식품학회지
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• 제35권3호
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• pp.360-369
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• 2015

Milk proteins have many potential sequences within their primary structure, each with a specific biological activity. In this study, we compared and investigated the bioactivities of hydrolysates of the domestic (A, B) and imported (C, D) skim milk powders generated using papain digestion. MALDI-TOF analysis revealed that all milk powder proteins were intact, indicating no autolysis. Electrophoretic analysis of hydrolysates showed papain treatment caused degradation of milk proteins into peptides of various size. The antioxidant activity of the hydrolysates, determined using 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and total phenolic contents (TPC) assays, increased with incubation times. In all skim milk powders, the antioxidant activities of hydrolysates were highest following 24 h papain treatment (TPC: A, 196.48 μM GE/L; B, 194.52 μM GE/L; C, 194.76 μM GE/L; D, 163.75 μM GE/L; ABTS: A, 75%; B, 72%; C, 72%; D, 57%). The number of peptide derived from skim milk powders, as determined by LC-MS/MS, was 308 for A, 283 for B, 208 for C, and 135 for D. Hydrolysate A had the highest antioxidant activity and the most potential antioxidant peptides amongst the four skim milk powder hydrolysates. A total of 4 β-lactoglobulin, 4 α_s1-casein, and 56 β-casein peptide fragments were identified as potential antioxidant peptides in hydrolysate A by LC-MS/MS. These results suggest that domestic skim milk could have applications in various industries, i.e., in the development of functional foods.

• Mass Spectrometry Letters
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• 제11권3호
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• pp.59-64
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• 2020

Mertansine, a thiol-containing maytansinoid, is a tubulin inhibitor used as the cytotoxic component of antibody-drug conjugates for the treatment of cancer. Liquid chromatography-tandem mass spectrometry was described for the determination of mertansine in rat plasma. 50-μL rat plasma sample was pretreated with 25 μL of 20 mM tris-(2-carboxyethyl)-phosphine, a reducing reagent, and further vortex-mixing with 50 μL of 50 mM N-ethylmaleimide for 3 min resulted in the alkylation of thiol group in mertansine. Alkylation reaction was stopped by addition of 100 μL of sildenafil in acetonitrile (200 ng/mL), and following centrifugation, aliquot of the supernatant was analyzed by the selected reaction monitoring mode. The standard curve was linear over the range of 1-1000 ng/mL in rat plasma with the lower limit of quantification level at 1 ng/mL. The intra- and inter-day accuracies and coefficient variations for mertansine at four quality control concentrations were 96.7-113.1% and 2.6-15.0%, respectively. Using this method, the pharmacokinetics of mertansine were evaluated after intravenous administration of mertansine at doses of 0.2, 0.5, and 1 mg/kg to female Sprague Dawley rats.

• Mass Spectrometry Letters
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• 제7권1호
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• pp.26-29
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• 2016

Simultaneous determination of three corticosteroids (clobetasol propionate, betamethasone dipropionate, fluticasone propionate) in moisturizers was performed by using liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). Sample preparation was conducted by the liquid-liquid extraction (LLE). Moisturizers include emulsifying agent and it forms micelles. In order to improve the extraction efficiency of corticosteroids trapped in micelle, newly developed-optimized extraction conditions which can remove the matrix effect from moisturizers was applied with various pH conditions in LLE extraction stage of sample preparation. Thus, the addition of 10 μL of 1 M HCl into moisturizers sample before extraction could improve the extraction efficiency. For the quantitative analysis, SRM table that contained specific transition of all of target corticosteroids was created. The developed method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantization (LOQ) and recovery. Over the 0.99 r² value was obtained in calibration standard range. Effective accuracy and precision were also obtained. LODs were below 31 ng/mL and LOQs were estimated below 94 ng/mL for all corticosteroids tested.

• 대한화학회지
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• 제59권3호
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• pp.225-232
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• 2015

하천수 중에 7가지의 농약류(2,4-dichlorophenoxyacetic acid, 메소밀, 알디캅, MCPA, molinate, 카바릴, 카보퓨란)와 2가지의 합성원료(quinoline, 비스페놀-A)를 액체크로마토그래피 전자분무 이온화 질량분석법(liquid chromatography-electrospray ionization-tandem mass spectrometry, LC-ESI-MS/MS)을 사용하여 분석하였다. 분석물질들은 고체상 추출법(solid-phase extraction, SPE)을 사용하여 추출하였다. 용리액은 질소가스로 농축하였다. 잔여물에 30% 메탄올 수용액 100 μL를 재 용해시켜 0.2 μm PTFE 실린지 필터로 거른 후 그 일부를 LC-ESI-MS/MS에 직접 주입하여 분석하였다. 확립된 조건에서 검정곡선은 0.997 이상의 상관계수를 갖는 직선성을 보였다. 정량한계는 0.002~0.011 μg/L 이었고, 정밀도는 16.4% 이하였다. 또한 정확도는 84~107%의 범위를 보였고, 회수율은 56.2~98.6% 값을 나타내었다. 본 연구에서 개발한 분석법을 실제 하천수 분석에 적용하였다.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.4023-4027
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• 2012

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (${\mu}FIC$). The $LC_{50}$ value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the ${\mu}FIC$ approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This ${\mu}FIC$ method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty-toxicity relationships.

• 한국수산과학회지
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• 제56권1호
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• pp.21-27
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• 2023

Lotus Nelumbo nucifera seed protein (LSP) was isolated by alkaline solubilization after removing fat and phenolics by hexane and ethanol treatment. Antioxidant peptides from LSP were produced with Alcalase^® and pepsin and hydroxyl radical scavenging activities were determined. LSP-Alcalase^® hydrolysates showed higher hydroxyl radical scavenging activity than LSP-pepsin hydrolysates. To purify antioxidant peptides, LSP-Alcalase^® hydrolysates were subjected to high performance liquid chromatography (HPLC) separation on the C18 column and the active fraction was further purified using a Superdex^TM peptide 10/300 GL column. Finally, the active fraction (F8-2) was evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging, and oxygen radical absorbance capacity (ORAC) assays. The EC₅₀ values of the F8-2 were 105.81±0.02 ㎍/mL for DPPH and 32.26±0.02 ㎍/mL for hydroxyl radical and the F8-2 exhibited 7.22 μM trolox equivalent (TE)/100 ㎍ F8-2. Glutathione (GSH), which is a positive control, showed EC₅₀ values of 19.87±0.01 ㎍/mL for DPPH and 15.95±0.03 ㎍/mL for hydroxyl radical and an ORAC value of 14.17±0.03 μM TE/100 ㎍ GSH. Finally, sixteen peptides were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, Ile-Tyr and Leu-Tyr showed higher antioxidant scores.