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Effects of Dietary Locally Grown Herbs (Mentha piperascens, Rubus coreanus, Tagetes patula) on the Growth Performance and Meat Quality of Broiler Chicken (국내 자생 약용식물자원(박하, 복분자 및 매리골드)의 첨가 급여가 육계의 생산성과 육질에 미치는 영향)

  • Kim, Yong-Ran;Lee, Bo-Keun;Kim, Jae-Young;Kim, Ji-Suk;Lee, Wan-Seob;Lee, So-Yeon;Kim, Eun-Jib;Ahn, Byoung-Ki;Kang, Chang-Won
    • Food Science of Animal Resources
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    • v.29 no.2
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    • pp.168-177
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    • 2009
  • This study was conducted to investigate the dietary effects of locally grown herbs (Mentha piperascens, PM; Rubus coreanus, RC; Tagetes patula, MG) on the growth performance and meat quality of broiler chicken. A total of 600 1-d-old Ross male broiler chicks were divided into eight groups and were fed control diets (antibiotics-medicated or non-medicated commercial diets) or experimental diets (non-medicated diets containing 0.3 or 0.5% PM, 0.3 or 0.5% RC, or 0.3 or 0.5% MG) for 5wk. The body weight gains and feed conversion rates (FCRs) in all the groups that were fed diets containing PM, RC, or MG were significantly improved compared to those in the non-medicated control group (p<0.05). The relative weights of various organs, the serum cholesterol levels, and the GOT/GPT activities in all the groups were also not significantly different. The superoxide dismutase (SOD)-like activities in the groups that were fed diets containing 0.3% PM or 0.3% MG were significantly increased compared to those in the non-medicated control group (p<0.05). The number of cecal lactic-acid bacteria in the groups that were fed diets containing 0.3% PM or 0.5% RC tended to increase. The malondialdehyde contents in the leg muscles were significantly lowered by feeding with PM or MG (p<0.05). The physicochemical properties of the edible meat, including the shear force, water-holding capacity, heat loss, pH, and muscle color degrees $(CIEL^*.\;a^*.\;b^*)$, were not affected by the dietary treatments. It has been suggested that spontaneous Korean plants promote the growth of broilers and may delay lipid oxidation in edible muscles without any negative effect when added in broiler diets.

Technical Efficiency of Medical Resource Supply and Demand (의료자원 공급, 수요의 성과 효율성에 대한 실증분석)

  • Chang, Insu;Ahn, Hyeong Seok;Kim, Brian H.S.
    • Journal of the Korean Regional Science Association
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    • v.34 no.2
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    • pp.3-19
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    • 2018
  • The objective of this study is to observe the efficiency of clinical performance on the supply and demand of medical resources in Korea. For the empirical analysis, we constructed the dataset on age standardized mortality rate, the number of physician, specialist, surgery, medical institution, ratio of general hospitals of 16 provinces in Korea from 2006 to 2013. The panel probability frontier model is employed as an analysis method and considered heteroscedasticity and autocorrelation of the error in panel data. In addition, the demographic and socioeconomic characteristics of the 16 provinces, unemployment rate, elderly population ratio, GRDP per capita, and ratio of hospitals in comparison to the general hospitals are used to find the effect on the technical efficiency of clinical performance on supply and demand of medical resources. The results are as follows. First, for the clinical performance, the supply side of human resources such as doctors and specialists and the demand side factors such as chronic illness clinic per unit population have a significant influence, respectively. Second, the technical efficiency of clinical performance on the supply and demand of medical resources of each input component was 59-70% in terms of clinical efficiency in each region. Third. estimates of technical efficiency of inputs that affect clinical performance showed a slight increase in all regions during the analysis period, but the increase trend decreased slightly. Fourth, the ratio of the elderly population and GRDP per capita have a positive influence on the technical efficiency of clinical performance on the supply and demand of medical resources. The difference of each efficiency by region is due to the regional differences of the input medical resources and the combination of them and the demographic and socioeconomic characteristics of the region. It is understood that the differences in technological efficiency due to the complexity of supply and demand of medical resources, demographic structure and economic difference affecting clinical performance by region are different.

Effects of Organic Iron Supplementation on Productivity and Egg Composition in Laying Hens (유기철의 첨가가 산란계의 생산성 및 난 성분에 미치는 영향)

  • 양철주;나상준;고석영;오종일;정대균;김해영;정일병;황보종;정완태
    • Korean Journal of Poultry Science
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    • v.31 no.2
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    • pp.101-108
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    • 2004
  • This study was conducted to evaluate the effect of iron reinforcing agent on the performance in laying hens for seven weeks. One hundred ninety eight 30-wk-old Brown Tetran layers were assigned to 9 treatments: control containing 45 mg Fe from iron sulfate per kg diet (FE-45), FE-90 (control multiplied two times), FE-180 (control multiplied four times), YM-45 containing 45mg Fe from yeast mutant, YM-90 (YM-45 multiplied two times), YM-180 (YM-45 multiplied four times), YF-45 containing 45mg Fe from ferritin containing yeast, YF-90 (YF-45 multiplied two times), YF-180 (YF-45 multiplied four times) in the experiment. Each treatment had four replications of 6 birds each. In the average egg production, the YF-180 was highest and the FE-45 (the control) was lowest; the YM-180, YF-90, and YF-180 were significantly (P<0.05) higher than the control. In the comparisons of egg weight and egg mass, the YF-180 was significantly higher (P<0.05) than the FE-90 or the control. In the FCR, the YM-90 was highest and the YF-45 was lowest. There was no significant difference between the control and YM-90 although the YF-45 was significantly (P<0.05) lower in FCR than the control. In the eggshell thickness, the YF-180 was the thickest and the FE-90 was the thinnest; the YF-45 or the YF-180 was significantly thicker (P<0.05) than the control. In the eggshell strength, the YF-45 was the strongest and the YM-45 was the weakest; the YM-90, YF-45, and YF-180 were significantly stronger (P<0.05) than the control. In the cholesterol level of egg yolk, the control (FE-45) was the highest and the YF-45 was the lowest; there was a significantly different (P<0.05) between these two treatments. In conclusion, in case of adding organic iron to feed for layers, it gives improvement on performance of layers and develop chemical composition of eggs.

Ecological Characteristics of Red Rice (Local Name "SARE," Oryza sativa L.) and Factors Affecting Its Competition with Rice (적미(赤米) (자생도(自生稻), 속명(俗名) "사레")의 생태적(生態的) 특성(特性) 및 벼와의 경합요인(競合要因))

  • Ree, D.W.;Hong, Y.K.;Kim, J.C.;Kim, Y.H.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.143-150
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    • 1983
  • Red rice (local name "Sore") as a weed has been a serious threat to rice production in direct-seeded rice culture in Ganghwagun, Gyeonggi province. In the Ganghwagun 508 ha out of 1,420 ha in the Samsanmyeon area is infested with red rice. The average lowland rice yield is about 4,300 kg/ha in the Ganghwagun, but the average upland rice yield is about 2,000 kg/ha in infested area. This study was carried out in order to clarify the ecological characteristics of red rice and factors affecting the competitive ability of five red rice varieties, collected from Samsanmyeon in 1981, with rice cultivar. Five varieties-Monggeunsare, Salsare, Ginkaragsare, Galsaegsalsare, Galsaegkaragsare-showed the same morphological characteristics of cultivated Japonica type, Chuncheongbyeo, but red rice tillers more profusely, is taller and lodges more easily than Chucheongbyeo. It shatters easily about 10-15 days after heading date, and at this time the hull is discolored in yellow or dark brown. There are many types of red rice with short or long owns on the spikelet, occasionally with or without own on the spikelet in the same hill, and the grains are short or long. In red reice leaf blast occurs more severely than in cultivated Indica/Japonica type, Teabaegbyeo, particulary serious in Monggeunsare. When red rice invaded in direct-seeded rice, number of panicles of rice became reduced more than other yield components.

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Changes in Weed Occurrence and Dominance Influenced by Seeding Dates in Water Seeded Rice Paddy under No-tillage Condition (벼 무경운(無耕耘) 담수표면산파재배시(湛水表面散播栽培時) 파종시기(播種時期)에 따른 잡초(雜草) 발생양상(發生樣相) 및 우점도(優點度) 변이(變異))

  • Kwon, O.D.;Guh, J.O.;Shin, H.R.;Park, T.D.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.179-185
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    • 1998
  • This study was conducted to investigate changes in weed occurrence and dominance as affected by seeding dates in water seeded rice paddy under field following two years of no-tillage condition. The pre-emergence weeds in no-tillage paddy were controlled by glyphosate application at 7 days before seeding. The rice seeds were seeded on April 20, May 10 and May 30, respectively with 40kg/ha of seeding rate. The weed species in the paddy occurred included three species (Echinochloa cursgalli, Aneilma keisak, Ottelia alismoides) of annual weed, one species (Alopecurus aequalis) of biennial weed and five species (Eleocharis kuroguwai, Leersia japonica, Sagittaria pygmaea, Sagittaria trifolia, Potamogeton distinctus) of perennial weed respectively. Aneilma keisak was found to be dorminant on April 20 and May 10 but Eleocharis kuroguwai on May 30 based on number of weeds. The number and dry weight of weeds occurred were higher on May 10 as compared to in other seeding dates, while proportion of perennial weeds increased as the seeding date was delayed. In particular, weed dorminance varied marketly with seeding date. The Simpson's indices were 0.736 on April 20, 0.261 on May 10 and 0.281 on May 30, respectively.

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Transformation of Potato using the Phosphinothricin Acetyltransferase Gene as the Selectable Marker Gene (감자의 형질전환을 위한 표지유전자로서 Phosphinothricin Acetyltransferase 유전자의 이용)

  • Jeong, J.H.;Yang, D.C.;Bang, K.S.;Han, S.S.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.205-213
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    • 1998
  • This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

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Effect of Submergence and Air Exposure of the Shoot on Growth, Nutrient Uptake and Photosynthesis in Monochoria vaginalis Presl. (물달개비 경엽(莖葉)의 침수여부(沈水與否)에 따른 생장(生長), 양분흡수(養分吸收) 및 광합성(光合成) 비교(比較))

  • Soh, C.H.;Yang, K.S.;Kwon, Y.W.
    • Korean Journal of Weed Science
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    • v.16 no.1
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    • pp.14-20
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    • 1996
  • Growth, nutrient uptake and photosynthesis as affected by submersion of shoot in pickerel weed (Monochoria vaginalis Presl.) were determined. The shoots of pickerel weeds in hydroponic culture were subjected to the submerged or emerged condition at 3- or 5-leaf stage for 8 or 10 days. Under submerged condition, growth in plant height was enhanced, but leaf number, leaf area, fresh and dry weight were reduced compared to those under the emerged condition. Similar responses in growth to submergence were obtained with the pickerel weeds rooted in the soil. Under submergence, chlorophyll content increased during the first 2 days, but thereafter remarkably decreased at 3-leaf stage and after the first 4 days at 5-leaf stage. Compared to the emerged condition, uptakes of $NH_4\;^+$-N, $NO_3\;^-$-N, $P_2O_5$ and $K^+$ were reduced, but uptakes of $Ca^{++}$ and $Mg^{++}$ increased under the submerged condition. Photosynthetic rate of shoot under water, measured by $CO_2$electrode, showed the maximum by 210 ${\mu}$moles $HCO_3\;^-$/g F.W. at the 8th day after submergence(DAS) at 3-leaf stage and 320 ${\mu}$moles $HCO_3\;^-$/g F. W. at 6 DAS at 5-leaf stage. These results indicate that pickerel weeds grow much better when the shoot is air-exposed and are less tolerable to submergence at 3 leaf-stage than at 5-leaf stage.

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Growth Characteristics and Green Manure Productivities of Hairy Vetch and Woolly Pod Vetch under Different Sowing Seasons in the Highland Area (고랭지에서 파종시기에 따른 헤어리베치와 울리포드베치의 생육특성 및 녹비생산성)

  • Ryoo, Jong-Won
    • Korean Journal of Organic Agriculture
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    • v.16 no.4
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    • pp.409-420
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    • 2008
  • The highland area remained bare for the winter and spring seasons and this condition has resulted in soil erosion. In this areas, crop rotation by legume has not been commonly practiced. Thus, this study was carried out to evaluate the effects of the growth characteristics and nitrogen yield as a green manure crop according to sowing seasons of hairy vetch and woolly pod vetch. In this experiment, we was investigated the growth characteristics, winter tolerance, soil coverage and productivities of hairy vetch and woolly pod vetch. We cultivated two hairy vetch(Madison, H1) and two woolly pod vetch(Naomi, Haymaker) varieties in the highland area from 2006 to 2007. The result are summarized as follows; The plant height of hairy vetch increased with the progress of growth stage until flowering stage. The wintering rate of hairy vetch was $79{\sim}83%$ in highland area. But, the wintering rate of woolly pod vetch was $29.5{\sim}39.2%$. The 100% soil coverage of hairy vetch was reached between end of May and beginning of June under autumn sowing, and between end of June and beginning of July under spring sowing. The weeds density of hairy vetch field under autumn sowing was lower than that under spring sowing. Dry matter yield of hairy vetch was significantly higher than that of woolly pod vetch under autumn sowing. The dry matter yield of hairy vetch and woolly pod vetch were $5,255{\sim}5,405kg/ha$ and $3,520{\sim}3,640kg/ha$, respectively. But, hairy vetch in spring sowing was not significantly higher fresh matter yield than that of woolly pod vetch. The nitrogen content in hairy vetch and woolly pod vetch was $2.98{\sim}3.08$ and $2.74{\sim}3.21%$, respectively. There were no significant difference in nitrogen content of the hairy vetch and woolly pod vetch under the spring sowing. N-uptake of hairy vetch was significantly higher than that of woolly pod vetch in autumn sowing. The N uptake was $156{\sim}164\;kg/ha$ in hairy vetch and $96{\sim}101\;kg/ha$ in woolly pod vetch under autumn sowing. It was thought that hairy vetch was better than woolly pod vetch as a green manure crop in autumn sowing considering wintering rate and yield of dry matter in highland area. And woolly pod vetch could use as a green manure crop under spring sowing in highland area.

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Antioxidant Activities and Biological Properties of Phellinus linteus Extracts according to Different Extraction Methods (추출방법에 따른 상황버섯 추출물의 항산화활성 및 생물학적 특성)

  • Kwoen, Dae-Jun;Youn, Sun-Joo;Cho, Jun-Gu;Choi, Ung-Kyu;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.49 no.2
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    • pp.91-96
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    • 2006
  • Antioxidant activities and biological properties such as antimutagenic and cytotoxic effect of Phellinus linteus extracts from different extraction conditions were measured against Salmonella typhimurium and human cancer cell lines. DPPH free radical scavenging activities of the extracts were higher in the solutions extracted with ethanol (17.14) and ethanol after water (17.79), respectively. In the Ames test, ethanol extract of P. linteus alone did not exhibit any mutagenicity but showed substantial inhibitory effect against mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (4NQO), and benzo $({\alpha})$ pyrene $(B({\alpha})P)$. The extracts of ethanol and ethanol after water of P. linteus $(200\;{\mu}g/plate)$ had the highest inhibitory effect of 61.5 and 60.9%, respectively, on the mutagenesis on S. typhimurium TA98 strain induced by $B({\alpha})P$. Extracted solutions of ethanol and ethanol after water of P. linteus showed high antimutagenic effect against MNNG, 4NQO, Trp-P-1 and $B({\alpha})P$, causing mutations in S. typhimurium TA100 strain. The anticancer effects of P. linteus extracts were investigated against human fibrosarcoma HT-29 and human hepatocellular carcinoma HepG2. The treatment of 0.5 mg/ml of ethanol, ethanol after water and water extracts of P. linteus had the highest cytotoxicity of 59, 57, 54%, respectively against HT-27 cell line, whereas low cytotoxicity effects were observed against HepG2 cell line in the range of $10{\sim}30%$. The ethanol and water extracts of P. linteus also showed the nitrate scavenging ability at different pHs. The ethanol extract showed higher nitrate-scavenging ability compared to water extract of P. linteus.

Japanese Medaka, Oryzias latipes as a Test Animal for Marine Ecotoxicological Evaluation (해양생태독성평가를 위한 표준시험생물로서의 송사리(Oryzias latipes)에 관한 연구)

  • Park Gyung Soo;Yoon Seong Jin;Lee Seung Min;Kim Ae Hyang;Park Soung Yun;Kang Duk Young
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.293-303
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    • 2005
  • Japanese medaka, Oryzias latipes is widely distributed in the North East Asia including Korea, Japan and east China, and commonly used for freshwater toxicity tests and cytotoxicological studies worldwide. In this study, a series of experiments were conducted to identify the potential of the fish as a standard test species for saltwater toxicity evaluation such as marine receiving waters, ocean-dumped materials and sediment pore waters etc. Hatching, growth and mortality rates of the fish were estimated with the wide ranges of salinity from freshwater to seawater (35 psu). Direct exposure of the fertilized eggs in freshwater to the wide ranges of salinity (from 0 to 35 psu) without pre- acclimation to the saltwater revealed no significant differences in hatching rates by salinities (p =0.24). On the other hand, medaka larvae hatched in freshwater and exposed to saltwater directly showed high mortality at > 25 psu treatment groups (p < 0.0001). However, there was no significant difference in mortality of medaka larvae hatched in 13.8 and 14.2 psu at the wide ranges of salinities ($0\~35$ psu). Growth rates of medaka larvae hatched in the above two salinities showed no differences in body length either from 0 to 35 psu treatment groups (p =0.64 for 13.8 psu group and p=0.32 for 14.2 psu group). The number of gill chloride cell in medaka larvae sharply increased when the larvae were exposed to high salinity. Reference tests with zinc chloride revealed 96h $LC_{50}=8.84(7.19\~10.87)mg\;L^{-1}$ using 7~10 day old medaka larvae. These were comparable or better sensitivity in comparison with the other standard test species such as North American sheepshead minnow Cyprinodon variegatus. Based on the results of these experiments, hatching rates and larvalmortality of medaka must be good toxicity parameters for seawater bioassay and the species seems to be a good standard species for both the freshwater and seawater toxicity test.