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Preincubation without attB DNA inhibits In Vitro Integrative Recombination of P 1 Mutant attP DNA of Bacteriophage Lambda

  • Yoo, Seung-Ku
    • Journal of Microbiology and Biotechnology
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    • v.5 no.3
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    • pp.132-137
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    • 1995
  • The lambda integrase (lnt) is believed to bind to several arm and core sites of attP DNA in order to facilitate intasome formation. We have done systematic mutagenic analysis on all 5 arm sites and found that P1 is absolutely required for integration while P2 is not. We also found that all 3 P' arm sites(P'1, P'2, and P'3) are required for efficient integrative recombination. P'1, which is an important binding site for excision, also seems to be crucial for integration when preincubation of attP DNA with Int and IHF is performed before recombination. Preincubation assay revealed that preincubation with Int and IHF improved the efficiency of recombination of wild type attP DNA and demolished recombinations of P'1 mutant attP DNAs.

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NON-TRIVIALITY OF TWO HOMOTOPY ELEMENTS IN π*S

  • Liu Xiugui
    • Journal of the Korean Mathematical Society
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    • v.43 no.4
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    • pp.783-801
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    • 2006
  • Let A be the mod p Steenrod algebra for p an arbitrary odd prime and S the sphere spectrum localized at p. In this paper, some useful propositions about the May spectral sequence are first given, and then, two new nontrivial homotopy elements ${\alpha}_1{\jmath}{\xi}_n\;(p{\geq}5,n\;{\geq}\;3)\;and\;{\gamma}_s{\alpha}_1{\jmath}{\xi}_n\;(p\;{\geq}\;7,\;n\;{\geq}\;4)$ are detected in the stable homotopy groups of spheres, where ${\xi}_n\;{\in}\;{\pi}_{p^nq+pq-2}M$ is obtained in [2]. The new ones are of degree 2(p - 1)($p^n+p+1$) - 4 and 2(p - 1)($p^n+sp^2$ + sp + (s - 1)) - 7 and are represented up to nonzero scalar by $b_0h_0h_n,\;b_0h_0h_n\tilde{\gamma}_s\;{\neq}\;0\;{\in}\;Ext^{*,*}_A^(Z_p,\;Z_p)$ in the Adams spectral sequence respectively, where $3\;{\leq}\;s\;<\;p-2$.

ON THE NORM OF THE OPERATOR aI + bH ON Lp(ℝ)

  • Ding, Yong;Grafakos, Loukas;Zhu, Kai
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.4
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    • pp.1209-1219
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    • 2018
  • We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky [7]: let H be the Hilbert transform and let a, b be real constants. Then for 1 < p < ${\infty}$ the norm of the operator aI + bH from $L^p(\mathbb{R})$ to $L^p(\mathbb{R})$ is equal to $$\({\max_{x{\in}{\mathbb{R}}}}{\frac{{\mid}ax-b+(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}ax-b-(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p}{{\mid}x+{\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}x-{\tan}{\frac{\pi}{2p}}{\mid}^p}}\)^{\frac{1}{p}}$$. Our proof avoids passing through the analogous result for the conjugate function on the circle, as in [7], and is given directly on the line. We also provide new approximate extremals for aI + bH in the case p > 2.

Efficient Processing of All-farthest-neighbors Queries in Spatial Network Databases

  • Cho, Hyung-Ju
    • Journal of Korea Multimedia Society
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    • v.22 no.12
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    • pp.1466-1480
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    • 2019
  • This paper addresses the efficient processing of all-farthest-neighbors (AFN) queries in spatial network databases. Given a set of data points P={p1,p2,…,p|p|} in a spatial network, where the distance between two data points p and s, denoted by dist (p,s), is the length of the shortest path between them, an AFN query is defined as follows: find the farthest neighbor ω(p)∈P of each data point p such that dist(p,ω(p)) ≥ dist(p,s) for all s∈P. In this paper, we propose a shared execution algorithm called FAST (for All-Farthest-neighbors Search in spatial neTworks). Extensive experiments on real-world roadmaps confirm the efficiency and scalability of the FAST algorithm, while demonstrating a speedup of up to two orders of magnitude over a conventional solution.

Design of Multiple VoIP Service Networks for P2P Network Environments based on Portable Internet (휴대인터넷 기반 P2P 망 환경에서의 다자간 VoIP 서비스 망 설계)

  • Eom Ki-Bok;Yoe Hyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.8
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    • pp.1640-1646
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    • 2004
  • In this study, we propose the multiple VoIP service networks for P2P network environments based on portable internet. We found that voiceservice using portable internet is possible for real time services. After we designe the multiple VoIP service networks for P2P network environments based on portable internet, evaluate the performances. from the performance evaluations, we found satisfactory results and so we can expect the possibility of the portable internet services.

pKT230 벡터를 이용한 Pseudomonas sp. P20의 2,3-Dihydroxybiphenyl Dioxygenase 유전자의 클로닝

  • Kim, Ji-Young;Kim, Chi-Kyung;Ka, Jong-Ok;Min, Kyung-Hee;Park, Yong-Keun
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.657-663
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    • 1996
  • Pseudomonas sp. P20 isolated from the polluted environment is capable of degrading biphenyl and 4-chlorobiphenyl. The pcbABCD genes responsible for degradation of biphenyl and 4-chlorobiphenyl were cloned using pBluescript SK(+) from the chromosomal DNA of Pseudomonas sp. P20 to construct pCK1 and pCK102, harbouring pcbABCD and pcbCD, respectively. The 2, 3-DHBP dioxygenase gene, pcbC, was cloned again from pCK102 by using pKT230 which is known as a shuttle vector and pKK1 hybrid plasmid was constructed. The E. coli KK1 transformant obtained by transforming the pKK1 into E. coli XL1-Blue showed 2, 3-DHBP dioxygenase activity. The specific 2, 3-DHBP dioxygenase activity of E. coli KK1 was similar to that of the E. coli CK102, but much higher than those of the natural isolates, Pseudomonas sp. DJ-12 and Pseudomonas sp. P20.

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Knowledge Integration and CoP Performance: Based on Social Capital and Diversity in CoP (CoP 내 지식통합과 CoP 성과 연구: 사회적 자본과 CoP 구성 다양성을 기반으로)

  • Lee, Gunho;Min, Jinyoung;Heo, Dongcheol;Lee, Junyeong;Lee, Heeseok
    • Knowledge Management Research
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    • v.15 no.2
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    • pp.129-145
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    • 2014
  • As a community of practice (CoP) is known to facilitate team learning, it is increasingly important to understand the mechanisms of CoP, thereby enabling organizations to fully utilize it and optimize its benefits. To explain how CoP improves organizational performance, we focus on its effects on social capital and knowledge management activities, and propose a research model suggesting that shared goals and trust in CoP improve its performance through knowledge integration. Our analysis uses structural equation modeling, with field data collected from 372 members of 46 CoPs in three companies; the analysis validates our research model. Our findings also suggest that CoP diversity can strengthen the link between knowledge integration and CoP performance.

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A Study of the Legacy Proxy Peer based on P2P Virtual Home Platform (P2P 가상홈 플랫폼 기반의 레가시 프락시 피어에 관한 연구)

  • Lee, Sang-Bong;Hwang, Tae-In;Oh, Byeong-Thaek;Kang, Shin-Yuk;Park, Ho-Jin
    • IEMEK Journal of Embedded Systems and Applications
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    • v.2 no.1
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    • pp.60-65
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    • 2007
  • 본 논문은 P2P 기반의 가상홈 미들웨어를 탑재할 수 없는 장치(이하 레가시 디바이스)를 P2P 인프라에 참여시키기 위한 방법에 관한 것으로, P2P 인프라와 레가시 디바이스 사이에 중계역할을 담당하는 레가시 프락시에 대한 연구이다. 가상홈 플랫폼은 기기 간 Seamless 연결을 제공하는 가상공간을 구성하여 언제 어디서나 P2P 방식으로 기기 간 서비스 및 콘텐츠를 용이하게 검색하고 공유하기 위한 기술로 JXTA 스펙을 따르고 있다. 레가시 프락시는 가상홈 미들웨어에 레가시 컨텐츠 공유 서비스, 가상홈 연계 서비스 및 레가시 디바이스 원격 제어 서비스를 추가한 것으로, 가상홈 인프라에 있는 임의의 피어가 레가시 디바이스의 컨텐츠 및 서비스를 이용하고 원격제어 할 수 있도록 해 준다.

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Study on the reaction mechanism between Sn and electroless Ni-P with varying P content (무전해 Ni-P막의 P 함량 변화에 따른 Sn과의 반응 메커니즘 연구)

  • ;;;Shih D. Y.
    • Proceedings of the International Microelectronics And Packaging Society Conference
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    • 2003.11a
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    • pp.88-93
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    • 2003
  • 무전해 Ni-P와 Sn의 반응 및 Ni-P의 결정화에 대한 P 함량의 영향을 세 가지 다른 조성의 Ni-P (P 4.6, 9, 13 $wt.\%$)를 이용하여 연구하였다. $300^{\circ}C$까지 열처리한 모든 시편에서 $Ni_3Sn_4$ intermetallic compound (IMC)가 생성되었고 이들 시편을 $450^{\circ}C$까지 열처리한 경우 Sn 두께가 $0.5{\mu}m$로 작을 때 $Ni_3Sn_4$가 모두 $Ni_3Sn_2$로 변화하였다. nanocrystal인 Ni-4.6P는 Ni (111) texture를 유지하며 결정화되었고 Sn과의 반응시 형성되는 $Ni_3Sn_4$ IMC 또한 비정질인 Ni-9P, Ni-13P 경우보다 강한 (111) texture를 가짐이 확인되었다. Ni-P 막의 P 함량이 작은 경우 $Ni_3Sn_4$ IMC는 두껍고 조밀하게 형성되는 반면 P-rich layer 두께는 작아졌다.

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A Novel Architecture of UPnP Bridge for Non-IP devices (Non-IP 장치 제어를 위한 새로운 UPnP 브리지 구조)

  • Kang, Jeong-Seok;Choi, Yong-Soon;Kim, Seong-Hoon;Lee, Kwang-Koog;Park, Hong-Seong
    • Proceedings of the KIEE Conference
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    • 2007.04a
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    • pp.442-444
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    • 2007
  • This paper presents an architecture of UPnP Bridge that allows controlling Non-iP devices from UPnP control point, without modification to Non-IP device or UPnP control point implementations. UPnP devices must provide SSDP discovery, SOAP control and GENA event processes. To represent Non-IP devices to UPnP devices, UPnP Bridge provides these functionalities on behalf of Non-IP devices. We provides two method to interoperability between UPnP and Non-IP devices, Message Field Description, Non-UPnP Proxy devices. And solution to integrate heterogeneous networking standards(RS232C, CAN, IEEE1394, USB) is provided.

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