• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.155-160
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• 2017

The dye decolorization rate in a cell-free culture broth of the white-rot fungus Trametes versicolor CBR43 was studied, including the effects of inhibitors of NaCl, Zn(II), and Cd(II) on dye decolorization activity. The maximum rates of dye decolorization in cell-free culture broth were 1,410, 44.7, 41.2, and $0.19{\mu}mol{\cdot}l^{-1}{\cdot}min^{-1}$ for Acid Blue 62, Acid Black 175, Reactive Blue 4, and Acid Red 114, respectively. The inhibition effects of NaCl, Zn(II), and Cd(II) on dye decolorization were quantitatively compared using the half maximal inhibition concentration ($IC_{50}$), which indicates the concentration of an inhibitor required for 50% inhibition. Based on $IC_{50}$ values, dye decolorization in the cell-free culture broth of CBR43 was most potently inhibited by Cd(II), whereas the inhibitory effect of NaCl was relatively low. The dye decolorization rates and $IC_{50}$ data can be used in the design and development of a dye-wastewater treatment process using T. versicolor CBR43 and its operating factors.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.84-91
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• 2017

The concern over the use of melittin in honey bee venom due to its adverse reaction caused by allergens such as phospholipase A2 ($PLA_2$) and hyaluronidase (HYA) has been an obstacle towards its usage. We developed a novel single-step method for melittin purification and the removal of $PLA_2$ and HYA. This study explores the influence of pH, buffer compositions, salt concentration, and types of cation-exchange chromatography resins on the recovery of melittin and the removal of both HYA and $PLA_2$. Melittin was readily purified with a strong cation-exchange resin at pH 6.0 with sodium phosphate buffer. It resulted in a recovery yield of melittin up to 93% (5.87 mg from a total of 6.32 mg of initial melittin in crude bee venom), which is higher than any previously reported studies on melittin purification. $PLA_2$ (99%) and HYA (96%) were also successfully removed. Our study generates a single-step purification method for melittin with a high removal rate of $PLA_2$ and HYA, enabling melittin to be fully utilized for its therapeutic purposes.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.488-492
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• 2016

S. fibuligera 균주는 생전분을 분해하는 활성이 있는 효모 균주이다. 본 연구에서는 첨가되는 젖산의 농도가 점차 증가하는 장기간 반복 회분식 배양 방법을 사용하는 적응진화를 통하여 S. fibuligera 균주의 젖산에 대한 내성을 증진시키고자 하였다. 진화된 균주인 S. fibuligera MBY1940 균주는 비성장속도 측정과 평판배지를 사용하는 성장분석을 통하여 모균주가 성장할 수 없는 젖산 농도 2.5%에 대해서도 내성을 나타내는 것을 확인하였다. 전자현미경 관찰을 통하여 젖산이 첨가된 스트레스 조건에서 모균주는 신장된 세포 형태와 세포막에 손상을 입은 것으로 보아 진화된 균주에 비해 젖산에 대하여 상대적으로 취약한 것으로 판단되었다.

• 한국산학기술학회논문지
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• 제13권4호
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• pp.1603-1608
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• 2012

실업의 규모는 실업상태에 진입하는 진입률의 크기와 실업상태의 지속기간이라는 두가지 요인에 의하여 결정된다. 본 연구는 실업의 지속기간을 측정하는 모형을 개발하여 다양한 형태의 실업자들의 실업지속기간을 측정하고자 한다. 분석결과 신규실업참가자의 실업지속기간과 실직자들의 실업지속기간의 결정요인은 서로 다르다는 것을 보인다. 모형을 이용하여 최근 대학 졸업자들이 노동시장에 신규로 참여하여 장기간 구직활동을 하는데 그 요인을 설명한다.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1606-1608
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• 2014

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

• Journal of Power Electronics
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• 제15권6호
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• pp.1601-1608
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• 2015

This paper presents a PQ control strategy for micro grid inverters with axial voltage regulators. The inverter works in the voltage-controlled mode and can help improve the terminal power quality. The inverter has two axial voltage regulators. The 1st regulator involves the output voltage amplitude and output impedance, while the 2nd regulator controls the output frequency. The inverter system is equivalent to a controllable voltage source with a controllable inner output impedance. The basic PQ control for micro grid inverters is easy to accomplish. The output active and reactive powers can be decoupled well by controlling the two axial voltages. The 1st axial voltage regulator controls the reactive power, while the 2nd regulator controls the active power. The paper analyses the axial voltage regulation mechanism, and evaluates the PQ decoupling effect mathematically. The effectiveness of the proposed control strategy is validated by simulation and experimental results.

• 한국자원식물학회지
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• 제23권6호
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• pp.503-512
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• 2010

Anther derived double haploids (DHs) from sweet pepper genotypes ('Special', 'Derby', 'Bossanova', 'Fiesta', 'Debora' and 'Minipaprika') were used to study the agronomic variation in 2006. Ninety-nine successful DHs regenerants (32 from 'Special', 25 from 'Derby', 23 from 'Bossanova', 10 from 'Fiesta', 6 from 'Debora' and 3 from 'Minipaprika') were transplanted at plastic house and studied on their agronomic characters. Variation in agronomic characters was observed within the DHs of each genotype. DHs obtained from 'Derby' and 'Fiesta' exhibited wide variation in fruit yield $plant^{-1}$ whereas averaged fruit yield $plant^{-1}$ was highest in 'Derby' (1608 g) and less variation was observed in DHs of 'Bossanova'. Based on the agronomic characters expressed in DHs population at this environment, SP55, SP56, SP60, and SP116 from 'Special', SP8, SP10, SP14, SP16, and SP34 from 'Derby', SP115, SP119, SP142, SP143, SP196, and SP199 from 'Bossanova', SP41, SP45, and SP114 from 'Fiesta', SP21 from 'Debora' and SP91 from 'Minipaprika' identified as elite inbred lines and these DH lines could be used for commercial hybrids production in sweet pepper. Genetic relationship among the selected inbred lines using molecular markers and their response to diseases are further recommended to study.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 C
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• pp.1606-1608
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• 2001

In connection with the monitoring of the insulation degradation of the large power apparatus, a statistical treatment, such as phase resolved partial discharge analysis (PRPDA) has been established for the on-line monitoring system during the past two decades. However, it has shown some inconveniences to distinguish the nature of the PD source in power apparatus. In this regard, a novel approach based on the chaotic analysis(CAPD) is proposed describing the fundamental ideas, outcomes and different view points from conventional PRPDA. In order to make a model for the possible defects causing sudden failures in service, several types of specimen were prepared. And partial discharge signals, originated from those samples, were measured and analyzed by means of PRPDA and CAPD respectively. In this paper, it seems that the correlation between the consecutive PD pulses depending on the nature of PD could be investigated by CAPD. Therefore, it could be considered that the nature of PD source can be distinguished more distinctively when the PRPDA is combined with CAPD.

• 복식
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• 제59권10호
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• pp.124-136
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• 2009

This Study is on the Punggongyubodoryake. Punggongyubodoryak was the records and pictures written by Ohgyeongmun(吳景文, Painter) and Gangbonpungeon(岡本豊彦, a Japanese painter, 1773~1745). in 1832. Those records and pictures were about the gifts which Korea's King(宣祖, 1567-1608) sent to Doyotomi Hideyosi(豊臣秀吉, Pungsinsugil) in 1590. Most of the gifts were of the Korean costume, which meant that Korea recognized Doyotomi Hideyosi as the new general of Japan, Tokugawa Shogunate(幕府將軍). The pictures of every Clothing in punggongyubodoryake described forms of every cloth and delineated ornamental patterns and sizes of clothing as closely as actual, they were clothes of the Middle period of Chosun. the author of the study inferred that it would be one of the impotent materials in the history of the Korean traditional costume. Among the clothes, there were several danryeongs(단령, ceremonial coat), okgwan (玉冠 woman headdress with) and paeok(佩玉, pendents with jade stings) and choongdan(中單 ceremonial undercoat) and Sang(裳, ceremonial Skirts for man), gyeontongsuseulran (肩通袖膝襕, chinese coat) was recorded in punggongyubodoryake. they were not a set of clothes for ceremonial costume but a mixture of men's and women's costume, of korean and abroad styles. the author inferred that this phenomenon was actually a good proof that the gifts were sent to Hideyosi only as courtesy, which meant for downgrading the receiver.

• 대한기계학회논문집A
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• 제21권10호
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• pp.1598-1608
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• 1997

Based on the application of te theory of conjugate approximations and the Loubignac's iterative method in a local region, a method to improve the stress filed in a displacement-formulated finite element solution has been proposed. The validity of the proposed method has been tested through two examples : a thick cylinder under internal pressure loading and an infinite plate with a central circular hole subjected to uniaxial tension. As a result of analysis of the examples, it was found that the stress field obtained for the local region model by the proposed method approximates well for the whole domain model. In addition, it was found that because of a significant decrease in the computing time to obtain the improved stress field, the proposed method is efficient and useful for the detailed stress analysis in local regions.