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http://dx.doi.org/10.4014/jmb.1608.08042

One-Step Purification of Melittin Derived from Apis mellifera Bee Venom  

Teoh, Angela Ching Ling (Korea University of Science and Technology (UST))
Ryu, Kyoung-Hwa (Biotechnology Process Engineering Centre, KRIBB)
Lee, Eun Gyo (Korea University of Science and Technology (UST))
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.1, 2017 , pp. 84-91 More about this Journal
Abstract
The concern over the use of melittin in honey bee venom due to its adverse reaction caused by allergens such as phospholipase A2 ($PLA_2$) and hyaluronidase (HYA) has been an obstacle towards its usage. We developed a novel single-step method for melittin purification and the removal of $PLA_2$ and HYA. This study explores the influence of pH, buffer compositions, salt concentration, and types of cation-exchange chromatography resins on the recovery of melittin and the removal of both HYA and $PLA_2$. Melittin was readily purified with a strong cation-exchange resin at pH 6.0 with sodium phosphate buffer. It resulted in a recovery yield of melittin up to 93% (5.87 mg from a total of 6.32 mg of initial melittin in crude bee venom), which is higher than any previously reported studies on melittin purification. $PLA_2$ (99%) and HYA (96%) were also successfully removed. Our study generates a single-step purification method for melittin with a high removal rate of $PLA_2$ and HYA, enabling melittin to be fully utilized for its therapeutic purposes.
Keywords
Melittin; purification; cation-exchange chromatography; hyaluronidase; phospholipase A2;
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