• Environmental Engineering Research
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• v.10 no.6
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• pp.283-293
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• 2005

Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, ${\mu}_{max}$, and the half velocity constant, $K_M$, were estimated as $0.61\;d^{-1}$ and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.127-131
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• 1986

In order to know the effects of iron the lipid characteristics in common feed, vitamin E and free unsaturated fatty acid concentrations were determined using feeds with(uncommon) and without(common) water added. The vitamin E concentrations in common feed(P<.05) and uncommon one (P<.01) showed statistically continuous decreasing figure from 1.75mg/g(1 day) to 0.25mg/g(11 days). The free unsaturated fatty acid concentrations in common feed showed also statistically continuous decreasing figure from 23.8mg/g(control group) to 17.6mg/g(20% ferrous sulfate group) (p<.01). On the mean time, quantitative values in uncommon feed have shown statistically continuous decreasing figure from 23.9mg/g(control group) to 17.4mg/g(20% ferrous sulfate group) (P<.01). The concentrations of vitamin E and free unsaturated fatty acid in common feed was higher than those in uncommon one, but significant difference was not recognized. The interactions between time lapse and ferrous sulfate were recognized in respect to the vitamin E and free unsaturated fatty acid in common and uncommon feed.

• The Mathematical Education
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• v.16 no.2
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• pp.22-26
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• 1978

It is shown that a map having an extension to an open map between the Alex-androff base compactifications of its domain and range has a unique such extension. J.S. Wasileski has introduced the Alexandroff base compactifications of Hausdorff spaces endowed with Alexandroff bases. We introduce a definition of morphism between such spaces to obtain a category which we denote by ABC. We prove that the Alexandroff base compactification on objects can be extended to a functor on ABC and that the compact objects give an epireflective subcategory of ABC. For each topological space X there exists a completely regular space $\alpha$X and a surjective continuous function $\alpha$$_{x}$ : Xlongrightarrow$\alpha$X such that for each completely regular space Z and g$\in$C (X, Z) there exists a unique g$\in$C($\alpha$X, 2) with g=g$^{\circ}$$\beta$$_{x}$. Such a pair ($\alpha$$_{x}$, $\alpha$X) is called a completely regularization of X. Let TOP be the category of topological spaces and continuous functions and let CREG be the category of completely regular spaces and continuous functions. The functor $\alpha$ : TOPlongrightarrowCREG is a completely regular reflection functor. For each topological space X there exists a compact Hausdorff space $\beta$X and a dense continuous function $\beta$x : Xlongrightarrow$\beta$X such that for each compact Hausdorff space K and g$\in$C (X, K) there exists a uniqueg$\in$C($\beta$X, K) with g=g$^{\circ}$$\beta$$_{x}$. Such a pair ($\beta$$_{x}$, $\beta$X) is called a Stone-Cech compactification of X. Let COMPT$_2$ be the category of compact Hausdorff spaces and continuous functions. The functor $\beta$ : TOPlongrightarrowCOMPT$_2$ is a compact reflection functor. For each topological space X there exists a realcompact space (equation omitted) and a dense continuous function (equation omitted) such that for each realcompact space Z and g$\in$C(X, 2) there exists a unique g$\in$C (equation omitted) with g=g$^{\circ}$(equation omitted). Such a pair (equation omitted) is called a Hewitt's realcompactification of X. Let RCOM be the category of realcompact spaces and continuous functions. The functor (equation omitted) : TOPlongrightarrowRCOM is a realcompact refection functor. In [2], D. Harris established the existence of a category of spaces and maps on which the Wallman compactification is an epirefiective functor. H. L. Bentley and S. A. Naimpally [1] generalized the result of Harris concerning the functorial properties of the Wallman compactification of a T$_1$-space. J. S. Wasileski [5] constructed a new compactification called Alexandroff base compactification. In order to fix our notations and for the sake of convenience. we begin with recalling reflection and Alexandroff base compactification.

• ALGAE
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• v.35 no.3
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• pp.277-292
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• 2020

Omega-3 fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are polyunsaturated fatty acids beneficial to human health. A limited number of microalgae have been used for commercial omega-3 production, which necessitates the identification of new microalgae with high omega-3 contents. We explored the fatty acid composition and EPA and DHA contents of the mixotrophic dinoflagellate Gymnodinium smaydae fed with the optimal algal prey species Heterocapsa rotundata. Cells of G. smaydae were found to be rich in omega-3 fatty acids. In particular, the DHA content of G. smaydae was 21 mg g^-1 dry weight, accounting for 43% of the total fatty acid content. The percentage of DHA in the total fatty acid content of G. smaydae was the highest among the reported microalgae except for Crypthecodinium cohnii. Moreover, to determine if the prey supply interval affected the growth rate of G. smaydae and its fatty acid content, three different prey supply intervals (daily, once every 2 d, and once for 4 d) were tested. Daily prey supply yielded the highest total fatty acid and DHA contents in G. smaydae. Furthermore, we successfully produced high-density G. smaydae cultures semi-continuously for 43 d with daily prey supply. During the semi-continuous cultivation period, the highest density of G. smaydae was 57,000 cells mL^-1, with an average growth rate of 0.7 d^-1. Taken together, the percentage of EPA and DHA in the total fatty acid content was maintained in the range of 54.2-56.9%. The results of this study support G. smaydae as a promising microalgal candidate for commercial DHA production and demonstrate that daily supply of prey can efficiently produce high-density G. smaydae cultures for more than a month.

• Journal of Life Science
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• v.16 no.6
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• pp.938-941
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• 2006

Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.

• Journal of Environmental Health Sciences
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• v.30 no.3
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• pp.230-238
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• 2004

The adverse health effects of a number of environment pollutions are related to the formation of free radicals. Induction of antioxidant defensive system in the response to an oxidative attack is an essential element of the cell to survive. CYP2E1 is easily induced by organic solvents and induces continuous formation of reactive oxygen species (ROS). ${\gamma}$-Glutamyltranspeptidase (${\gamma}$GT) plays an important role in glutathione metabolism and xenobiotic detoxification. To evaluate the characteristic of oxidative stress which induces GGT expression and to understand human antioxidant defensive response against oxidative stress induced by CYP2E1, we studied regulation of ${\gamma}$GT enzyme expression in response to various oxidative stresses in human HepG2 cells. The ${\gamma}$GT activity was not modified after exposure of acute oxidative stress inducing agents (ferric nitrilotriacetate, cumene hydroperoxide, ADP-Fe, O-tetradecanoylphorbol-13-acetate, tumor necrosis factor-alpha). To induce continuous exposure of cells to ROS, HepG2 cells were transfected by human CYP2E1 gene transiently. The CYP2E1 activity was verified with chlorzoxazone hydroxylation. Transfection of CYP2E1 showed continuous 60% increase in intracellular ROS and 240 % increase in microsomal ROS. CYP2E1 overexpressing cells showed increased ${\gamma}$GT activity (2.5-fold). The observed enhancement of ${\gamma}$GT activity correlated with a significant increase of ${\gamma}$GT mRNA (2.1-fold). Treatment with antioxidant strongly prevented the increase in ${\gamma}$GT activity. The CYP2E1 overexpression did not modify toxicity index and increased glutathione levels. These results show that continuous exposure of cells to ROS produced by CYP2E1 up-regulates ${\gamma}$GT; This may be one of the adaptive antioxidant responses of cells to oxidative insult. Present study also suggests that the induction of ${\gamma}$GT could be used as a marker of oxidative stress induced by exposure to organic solvents.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.482-487
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• 1999

The Pseudomonas sp. strain NGK1 (NCIM 5120) was immobilized in calcium alginate, agar, and polyacrylamide gel matrices. The salicylic acid-producing capacity of freely suspended cells was compared with immobilized cells in batches with a shake culture and continuous culture system in a packed bed reactor. Freely suspended cells ($4\times10^{10}cfu/ml$) produced 12 mM of salicylic acid, whereas cells immobilized in calcium alginate ($1.8\times10^{11}$cfu/g beads), agar ($1.8\times10^{11}$cfu/g beads), and polyacrylamide ($1.6\times10^{11}$cfu/g beads) produced 15, 11, and 16mM of salicylic acid, respectively, from naphthalene at an initial concentration of 25 mM. The continuous production of salicylic acid from naphthalene was investigated in a continuous packed bed reactor with two different cell populations. The longevity of the salicylic acid-producing activity of the immobilized cells from naphthalene was also studied in semi continuous fermentations. The immobilized cells could be reused 18, 13, and more than 20 times without losing salicylic acid-producing activity in calcium alginate-,agar-, and polyacrylamide-entrapped cells, respectively. The study reveals a more efficient utilization of naphthalene and salicylic acid production by the immobilized Pseudomonas sp. strain NGK1 as compared to the free cells.

• Journal of the Korean Operations Research and Management Science Society
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• v.19 no.3
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• pp.235-241
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• 1994

We consider a B/G/1 queueing with vacations, where the server closes the gate when it begins a vacation. In this system, customers arrive according to a Bernoulli process. The service time and the vacation time follow discrete distributions. We obtain the distribution of the number of customers at a random point in time, and in turn, the distribution of the residence time (queueing time + service time) for a customer. It is observed that solutions for our discret time B/G/1 gated vacation model are analogous to those for the continuous time M/G/1 gated vacation model.

• Bulletin of the Korean Mathematical Society
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• v.33 no.2
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• pp.205-211
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• 1996

Throughout this paper, we will work in the category of compact connected manifolds and continuous maps. If $X_1, X_2$ are manifolds, $f, g: X_1 \to X_2$ are maps, then a point $x \in X_1$ is a coincidence of f and g iff f(x) = g(x) ; the set of all such points is denoted by Coin(f,g).

• Proceedings of the Materials Research Society of Korea Conference
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• 1992.05a
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• pp.9-9
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• 1992