• Title/Summary/Keyword: (PS) condition

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Transformation of Rhizobacteria Pseudomonas fluorescens by Electroporation (Electroporation에 의한 근권 미생물 Pseudomonas fluorescens의 형질전환)

  • Kim, Jong-Hyun;Rhee, Young-Hwan
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.371-375
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    • 1995
  • The antagonistic rhizobacteria Pseudomonas(P.) fluorescens against F. oxysporum and R. solani were isolated and selected, and then, their biological and physiological characteristics were investigated. The posibility and optimum condition of the electroporation of antagonistic rhizobacteria with Ps70, one of the selected one, and plasmid pSV2-neo was studied. Its optimum condition was found with HGEB which contains 1 mM (pH 7.0) hepes and 10% glycerol at setting of 200 resistance, 25 ${\mu}F$ capacitance, and 2.5 kV applied voltage. In addition, the transformation efficiency obtained with pSV2-neo was compared to other plasmids with different sizes. The applied voltage, the buffer composition and the parallel resistor (time constant) were shown to have the greatest effect on transformation efficiency in electroporation. And the rest of the selected rhizobacteria were also successfully transformed with pSV2-neo by electroporation.

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Preparation and Characteristic of Sheet Molding Compound using Unsaturated Polyester Resin with Low Profile Agent of Polystyrene (저수축제 폴리스틸렌과 불포화 폴리에스터 수지를 사용한 Sheet Molding Compound 제조 및 특성)

  • Bae, Gi Boong;Lee, Sang Goo;Yoon, Hong Jin;Lee, Jong Dae
    • Korean Chemical Engineering Research
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    • v.50 no.3
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    • pp.588-593
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    • 2012
  • Compatibility of unsaturated polyester (UP) and low profile agent (LPA) of polystyrene (PS) have been investigated under various mixing conditions such as the ratio of UP and LPA, mixing time, mixing temperature, and input amount of 2nd UP. It was possible to obtain mixture with small particle size and low phase separation in condition of 35 g of LPA, 25 g of 1st UP input, 5 min of mixing time, 1700 rpm of mixing speed, and 45 g of 2nd UP input. It was found that compatibility of UP and LPA was very sensitive to mixing conditions. In addition, molded sample using sheet molding compound prepared by stable mixing condition appeared good properties such as low water adsorption, low shrinkage, and high gloss.

Cultural Condition of Pseudomonas mendocina for Polysaccharide Production (Pseudomonas mendocina 에 의한 Polysaccharide 생산)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.21 no.5
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    • pp.619-623
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    • 1989
  • The cultural condition of Pseudomonas mendocina for polysaccharide production was examined. The optimal medium contains following composition per liter of distilled water: Sucrose 23.75g, $(NH_4)_2SO_4$ 1.57g, Yeast extract 0.5g, $KH_2PO_4\;2.9g,\;MgSO_4.\;7H_2O\;1.0g,\;CaCO_3$ 2.5g. The optimum temperature and pH were $30^{\circ}C$ and 6.5. At the condition. Ps mendocina produced 5.98g/l of polysaccharide. The culture viscosity after 3 days was 191mPa.s at $70sec^{-1}$. The product yield $(Y_{p/s})$ and specific productivity $(Q_p)$ were 25.18% and 32.83mg/g-cell/h.

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A Study on the Operation Performance of Diesel Engine by using of Soybean Oil Fuel (디젤엔진의 콩기름연료에 의한 운전성능에 관한 시험)

  • 이기명
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.18 no.4
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    • pp.4259-4264
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    • 1976
  • This paper, is about the test on the operating performance of diesel engine by using of soybean oil which farmers could supply in their farm yard. The diesel engine used is a swirl-chamber type, four stroke cycle with single cylinder, air cooling and its rated horse power is 2 PS per 1300 rpm. Several results obtained are as follows; 1. The starting performance of diesel engine with soybean oil is almost the same as that with light oil. 2. The variation of engine speed according to various engine load is small when soybean oil is used compared with light oil. It is considered that soybean oil is desirable for the purpose of industerial power machine fuel. 3. The specific fuel consumption increases approximately 10 percent high in the condition of rated horse power and maximum horse power and shows less or same during the load test in low velocity, when soybean oil is used 4. Though the brake thermal efficiency in the condition of rated horse power and maximum horse power is inclined to decrease when soybean oil is used compared during the load test in low velocityt shows good inclination.

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Single-mode Condition and Dispersion of Terahertz Photonic Crystal Fiber

  • Kim, Soan;Kee, Chul-Sik;Lee, Jong-Min
    • Journal of the Optical Society of Korea
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    • v.11 no.3
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    • pp.97-100
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    • 2007
  • We have investigated properties of a plastic photonic crystal fiber guiding terahertz radiations, THz photonic crystal fiber. The single-mode condition and dispersion of a plastic triangular THz photonic crystal fiber are investigated by using the plane wave expansion method and the beam propagation method. The THz photonic crystal fiber can perform as a single-mode fiber below 2.5 THz when the ratio of diameter (d) and period (${\Lambda}$) of air holes is less than 0.475. The THz photonic crystal fiber with ${\Lambda}=500{\mu}m$ and $d/{\Lambda}=0.4$ shows almost zero flattened dispersion behavior, $-0.03{\pm}0.02 ps/THz{\cdot}cm$, in the THz frequency range from 0.8 to 2.0 THz.

A Study on the Wind Power Generation Using Vertical Exhaust Air Duct of the High-Rise Apartments (초고층 공동주택의 주방.욕실 배기 풍속을 풍력발전에 활용하는 방안)

  • Lee, Yong-Ho;Kim, Seong-Yong;Hwang, Jung-Ha;Park, Jin-Chul
    • Journal of the Korean Solar Energy Society
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    • v.32 no.3
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    • pp.1-10
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    • 2012
  • The purpose of this study was to promote the utilization of wind velocity of kitchen and bathroom exhaust ducts for wind power generation in high-rise apartments. The research content can be summarized as follows: 1) Nine high-rise apartments were examined for the installation of kitchen and bathroom exhaust ducts located in the pipe shaft (PS) section. After selecting simulation candidates, a simulation was performed with the STAR-CCM+ Ver 5.06 program. 2) Of nine high-rise apartments, seven had kitchen and bathroom exhaust ducts, whose cross section was in the range of $0.16m^2{\sim}0.4m^2$. The area ratio between the exhaust ducts and PS section (cross section of exhaust duct/area of PS section ${\times}$ 100) was on average 3.2%. 3) The simulation results were analyzed. As a result, the smaller cross section kitchen and bathroom exhaust ducts had, the more advantages there were for increasing exhaust wind velocity. If an out air inlet duct is installed to the old kitchen and bathroom exhaust ducts, it will increase exhaust wind velocity by 3.01~3.98m/s and contribute to the proper wind velocity level (3.0m/s). 4) When the simultaneous usage rate between the kitchen and bathroom exhaust fan increased from 20% to 60%, exhaust wind velocity increased. The "entire house holds" condition for exhaust fan operation provided more even exhaust wind velocity than the "some house holds" condition. 5) Exhaust wind velocity increased in the order of amplified (T-3), induced (T-2) and vertical (T-1) top of kitchen and bathroom exhaust ducts. Of them, the amplified type (T-3) was under the least influence of external wind velocity and thus the most proper for kitchen and bathroom exhaust duct tops.

Polarization-sensitive Optical Coherence Tomography Imaging of Pleural Reaction Caused by Talc in an ex vivo Rabbit Model (생체 외 토끼 모델에서의 탈크에 의해 유발되는 흉막 반응의 편광 민감 광 결맞음 단층촬영 이미징)

  • Park, Jung-Eun;Xin, Zhou;Oak, Chulho;Kim, Sungwon;Lee, Haeyoung;Park, Eun-Kee;Jung, Minjung;Kwon, Daa Young;Tang, Shuo;Ahn, Yeh-Chan
    • Korean Journal of Optics and Photonics
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    • v.31 no.1
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    • pp.1-6
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    • 2020
  • The chest wall, an organ directly affected by environmental particles through respiration, consists of ribs, a pleural layer and intercostal muscles. To diagnose early and treat disease in this body part, it is important to visualize the details of the chest wall, but the structure of the pleural layer cannot be seen by chest computed tomography or ultrasound. On the other hand, optical coherence tomography (OCT), with a high spatial resolution, is suited to observe pleural-layer response to talc, one of the fine materials. However, intensity-based OCT is weak in providing information to distinguish the detailed structure of the chest wall, and cannot distinguish the reaction of the pleural layer from the change in the muscle by the talc. Polarization-sensitive OCT (PS-OCT) takes advantage of the fact that specific tissues like muscle, which have optical birefringence, change the backscattered light's polarization state. Moreover, the birefringence of muscle associated with the arrangement of myofilaments indicates the muscle's condition, by measuring retardation change. The PS-OCT image is interpreted from three major perspectives for talc-exposure chest-wall imaging: a thickened pleural layer, a separation between pleural layer and muscle, and a phase-retardation measurement around lesions. In this paper, a rabbit chest wall after talc pleurodesis is investigated by PS-OCT. The PS-OCT images visualize the pleural layer and muscle, respectively, and this system shows different birefringence of normal and damaged lesions. Also, an analyisis based on phase-retardation slope supports results from the PS-OCT image and histology.

The Optimum Operating Condition for Reduction of Fuel Consumption -In the Case of Training Ship Pusan 402- (연료 절감을 위한 최적운전 조건 -신습선 부산 402호의경우-)

  • Kim, Yeong-Sik;Kim, Sam-Kon;Yoon, Suck-Hun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.22 no.1
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    • pp.29-33
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    • 1986
  • This paper aims at finding out the optimum operating condition to reduce fuel consumption for the training ship Pusan 402 with controllable pitch propeller. For this purpose, this paper examints the variation of ship speed and fuel consumption in accordance with the change of engine revolution and propeller pitch. The results obtained are as follows: 1. When engine revolution is constant, the ship speed sluggishly increases according to the increase of propeller pitch but fuel consumption extremely increases. The higher revolution the engine is, the more remarkable this tendency is. 2. As the engine revolution becomes lower, the fuel consumption per mile decreases. Howt.er = the fuel consumption under the same engine revolution differs according to the propeller pitch. 3. Specific fuel consumption is uniformed about 180g/ps.h at any case of load. 4. Among the various operating conditions which yield the same ship speed, fuel consumption lowers in the case of lower engine revolution and larger propeller pitch.

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Characteristics of the Concentration Process of Lactobacillus Cell Using a Ceramic Membrane (세라믹막을 이용한 Lactobacillus cell의 농축 공정의 특성)

  • Lee Yong Taek;Song Min-Ho
    • Membrane Journal
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    • v.14 no.3
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    • pp.192-200
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    • 2004
  • It is an anaerobic germ that Lactobacillus cell concentrated using ceramic membrane has high stability and long lifetime as compared with polymeric membrane. The effects of operating pressure, temperature, crossflow velocity on cell harvesting have been studied. Also the variation of flux and transmembrane pressure (TMP) with increasing concentration ratio and the change of TMP at constant concentration ratio (volumetric concentration factor: VCF) regarding the optimization have been examined. It showed that the permeate flux increased gradually with the increasing of transmembrane pressure, crossflow velocity, and volumetric concentration factor. The higher initial flux was due to the reduction of viscosity at elevated temperature. However, as operating time progressed, the effect of temperature was negligible since the effect of viscosity became minor. As a result, that operate in a constant concentration ratio, decreased degree could know that become slowly although the flux decreases according as operating time progressed. The flux is a very stable in the condition of constant VCF range. The yield of Latobaciilus (PS 406) which was cultivated at $37^{\circ}C$ was concentrated about 4.9{\times}10^9$ after operation.

Tests on Transfer Bond Performance of Epoxy Coated Prestressing Strands (에폭시 코팅 처리된 PS강선의 정착부착성능 실험)

  • 유승룡
    • Magazine of the Korea Concrete Institute
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    • v.6 no.1
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    • pp.89-100
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    • 1994
  • The current test procedure for transfer length, which determine transfer length by measuring concrete strain, has an actual bond stress state in the prestressed pretensioned member : however, it is difficult to determine the bond properties of maximum bond stress and bond stiffness with this method. It is also difficult for design engineer to understand and select a correct safety criterion from the widely distributed results of such a ransfer test alone. An alternative testing procedure is provided here to determine the bond properties without measuring the concrete strain. In this test the bond stress is measured directly by creating a similar boundary condition within the transfer length in a real beam during the transfer of prestressing force. The prestressing force was released step by step by step from the unloading side. The release of force induces a swelling of the strand at the unloading side of concrete block, bonding force in the block, and a bond slip of the strand toward the other side of the block. Two center-hole load cells are used to record the end loads until the point of general bond slip(maximum bond stress). It is suggested that this test procedure be performed with the ordinary transfer test when determining the transfer length in a prestressed, pretensioned concrete beam.