• 한국작물학회지
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• 제38권4호
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• pp.317-323
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• 1993

화성억제재배한 참당귀(Angelica gigas Nakai)의 근에서 약효성분함량을 구명하기 위하여 주요약효성분인 조추출물(엑스), Decursin 및 Decursinol angelate 등을 정량분석하여 연근ㆍ부위 및 생육단계별로 비료하였고, 또한 추대와 개화에 따른 근의 본질화 진행특성을 조사하기 위하여 목질 화세포, 중심주의 무게와 반경 및 피층부의 무게와 두께에 대하여 조사하였던 바 얻어진 일련의 겨과를 요약하면 다음과 같다. 1. 년근에 따라 엑스함량은 1, 2, 3년근이 모두 50% 내외로 큰 차이가 없었으나 Decursin과 Decursinol angelate 함량은 각각 8.20%와 5.01%로서 3년근이 가장 높은 함량을 보였다. 2. 근의 부위별 약효성분함량은 세근이 가장 많았고, 다음은 기근, 주근순으로 적어졌으며, 함량은 피층부가 중심계보다 엑스, Decursin, Decursinol angelate 모두 월등히 많았다. 또한 수확시기가 늦어질수록 약효성분함량이 증가하였다. 3. 생육단계가 진행됨에 따라 약효성분함량은 감소하였고, 목질화세포가 중심계의 무게와 반년이 증가하는 반면에 피층부의 무게와 두게는 상대적으로 감소하였다.

• Applied Biological Chemistry
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• 제29권4호
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• pp.381-391
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• 1986

토양중(土壤中) 무기양분함량(無機養分含量) 및 흡수량(吸收量)이 당귀(當歸)의 유효성분함량(有效成分含量)에 미치는 영향(影響)을 조사(調査)하여 재배산(栽培産) 당귀(當歸)의 유효성분함량(有效成分含量)을 증대(增大)시켜 품질(品質)이 좋은 당귀근생산(當歸根生産)을 위한 기초자료(基礎資料)로 이용(利用)하고자 산지별(産地別)로 재배산(栽培産) 및 야생산(野生産)을 토양(土壤)과 동시(同時)에 채취(採取)하여 토양(土壤)및 당귀근내(當歸根內)의 무기양분함량(無機養分含量)과 decursin, decursinol 함량(含量)과의 상관관계(相關關係)를 조사(調査)하였다. 당귀근중(當歸根中) decursin 함량(含量)은 봉화산(奉化産)이 6.06%, 진부산(産)이 5.86%, 월성산(月城産)이 3.24%이었으며 야생산(野生産)은 7.65%로서 재배산(栽培産) 보다 그 함량(含量)이 더 높았다. decursin 함량(含量)은 토양중(土壤中) ${NH_4}^+-N$ 함량(含量)과 부(負)의 상관(相關)이 유효태(有效態) 인산함량(燐酸含量)과 정(正)의 상관(相關)이 인정(認定)되었다. decursin 함량(含量)은 당귀근중(當歸根中) 질소함양(窒素含量)과 부(負)의 상관(相關)이, 인산(燐酸) 및 가리함량(加里含量)과 정(正)의 상관(相關)이 인정(認定)되었다. 그러므로 당귀(當歸)의 유효성분(有效成分)인 decursin 함량(含量)은 토양(土壤) 및 당귀근(當歸根) 중(中)의 질소함량(窒素含量)이 적을수록 인산(燐酸), 가리함량(加里含量)이 많을수록 증가(增加)하는 경향이었다.

• 한국식품위생안전성학회지
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• 제34권1호
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• pp.52-57
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• 2019

본 연구에서는 건강기능성 식품 소재 개발을 목적으로 참당귀 지상부 추출물의 표준화를 위하여 decursin을 지표성분으로 선정하고 HPLC분석을 통하여 decursin의 분석법을 확립하고 유효성 검정을 수행하였다. 유효성 검정 결과, decorsin 표준용액과 참당귀 지상부 추출물의 retention time 및 spectrum이 일치하는 특이성을 확인하였다. 표준 검량선의 상관계수($R^2$)는 0.9994로 매우 우수한 직선성을 나타내었으며 정량한계(LOQ)는 $0.034{\mu}g/mL$이었고 검출한계(LOD)는 $0.011{\mu}g/mL$이었다. Decursin의 intra-day 분석에서 정확성 및 상대표준편차는 각각 96.49 ~ 104.35%, 0.53 ~ 1.10%, inter-day 분석에서 정확성 및 상대표준편차는 각각 97.23 ~ 100.14%, 0.26 ~ 1.15%인 것으로 나타나 본 시험법의 정확성 및 정밀성을 확인 할 수 있었다. 표준품 농도별 지표성분의 회수율은 92.38 ~ 104.11%의 범위였으며 분석오차 10% 이내로 측정되었다. 이는 본 연구에서 수행된 HPLC 분석법이 참당귀 지상부 추출물의 지표성분인 decursin을 분석하고 표준화하는데 적합한 시험법임을 의미한다.

• Natural Product Sciences
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• 제10권2호
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• pp.89-91
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• 2004

Six components were isolated from the $CH_2Cl_2$ fraction of Belamcanda chinensis rhizome by open column chromatography. Their structures were elucidated as ${\beta}-sitosterol$ (1), apocynin (2), decursin (3), iristectorigenin A (4), irigenin (5) and tectorigenin (6) by spectral analysis. Among these compounds, decursin (3) was isolated for the first time from a plant of the family Iridaceae.

• Natural Product Sciences
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• 제9권3호
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• pp.170-173
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• 2003

The anti-oxidant activities of decursinol angelate (1) and decursin (2) isolated from Angelica gigas were investigated. These two coumarins exhibited decrease in serum transaminase activities elevated by hepatic damage induced by $CCl_4-intoxication$ in rats. They also showed increase in anti-oxidant enzyme such as hepatic cytosolic superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-px) in $CCl_4-intoxicated$ rats. These results suggest that decursinol angelate (1) and decursin (2) from A. gigas possess not only the anti-oxidant, but also the hepatoprotective activities in rats.

• 생약학회지
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• 제39권2호
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• pp.86-90
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• 2008

Two decursin derivatives were synthesized from decursinol. Compounds 1 and 2 were determined as 7-{3-[4-(2-Methoxy-phenyl)-piperazin-1-yl]-propoxy}-8,8-dimethyl-7,8-dihydro-6H-pyranochromen-2-one (1) and decursinol 3'-O-E-pmethoxycinnamic acid ester (2), respectively and newly reported. Compounds 1 and 2 significantly inhibited AChE activity and ameliorated memory impairment induced in mice by scopolamine (1.0 mg/kg body weight s.c.) as measured in passive avoidance test. We suggest, therefore, that compounds 1 and 2 has both anti-AChE and anti-amnesic activities that may ultimately hold significant therapeutic value in alleviating certain memory impairment observed in Alzheimer's disease.

• Archives of Pharmacal Research
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• 제26권9호
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• pp.727-730
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• 2003

The in vivo anti-tumor activities of decursinol angelate (1) and decursin (2) isolated from the roots of Angelica gigas were investigated. These two compounds, when administered consecutively for 9 days at 50 and 100 mg/kg i.p. in mice, caused a significant increase in the life span and a significant decrease in the tumor weight and volume of mice inoculated with Sarcoma-180 tumor cells. These results suggest that decursinol angelate (1) and decursin (2) from A. gigas have anti-tumor activities.

• 약학회지
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• 제55권4호
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• pp.301-308
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• 2011

Diabetes has been one of major health risks in industrialized countries. AMP-activated protein kinase (AMPK) has been focused as a novel therapeutic target for the treatment of metabolic syndromes, because AMPK increases glucose uptake through independent insulin signal pathway. In this study, we investigated the anti-diabetic effect of Angelica gigas Nakai extract (AGNEX), a mixture of decursin and decursinol angelate (53 : 47), decursin and decursinol angelate on blood glucose, glucose transport (GLUT) and AMPK expression levels in streptozotocin (STZ)-induced diabetic rats. To induce diabetes, 50 mg/kg of STZ was injected via i.v. route and AGNEX 2 mg/kg (STZ+AG), decursin 2 mg/kg (STZ+D), decursinol angelate 2 mg/kg (STZ+DA), and metformin 100 mg/kg (STZ+M) were administered orally for 21 days. STZ+DA group showed a significant decrease in fasting blood glucose levels compared to the other groups. Decursinol angelate significantly upregulated expression of glucose transporter 4 (GLUT4) and phosphorylation of AMPK (p-AMPK) in skeletal muscle of rats. In pancreas of rats, decursinol angelate significantly increased expression of GLUT2 through down-regulation of p-AMPK. In addition to the result of pancreatic islets morphology, AGNEX, decursin, decursinol angelate, and metformin treated group recovered ${\beta}$-cell damage by hyperglycemia. These results indicate that decursinol angelate might be a potential anti-diabetic agent and AGNEX could be useful in the treatment of diabetes mellitus.

• 한국약용작물학회지
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• 제17권6호
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• pp.458-463
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• 2009

This study was conducted to find out some conditions for optimum cultivation of Angelica gigas Nakai by the investigation of root yield and decursin content from different seeding sizes and accumulative temperature. Accumulative temperature from April to October was $4,309^{\circ}C$ on altitude 100 m, $4,242^{\circ}C$ on 250 m, $3,662^{\circ}C$ on 530 m, $3,435^{\circ}C$ on 730 m, and altitude 530~730 m was less $650{\sim}870^{\circ}C$ than altitude 100 m cultivation areas of A. gigas. Seedling stand rate was increased from 86.4% to more than 90% as accumulative temperature decreases, and was increased in above 7 mm of seedling size, and 10% in non-mulching more than PE film mulching. Yield was increased as accumulative temperature decreases and in PE film mulching as 310.2 kg/10a. Also, Yield was increased in 7~9 mm than seedling diameter 5~7 mm as 313.0 kg/10a. Decursin content of primary roots was increased as altitude rises, ie, as 2.55% on altitude 100 m, 3.33% on 250 m, 5.51% on 530 m, and 6.24% on 730 m. Decursinol angelate content appeared little than decursin content as 1.08% on altitude 100 m, 1.37% on 250 m, 1.99% on 530 m, 2.38% on 730 m, and as altitude was heightened, content was increased.

• 생약학회지
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• 제34권3호통권134호
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• pp.201-205
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• 2003

This paper is intended as an investigation of the analysis of high-performance liquid chromatography and the method of extraction of decursin and decursinol angelate in Angelica gigas roots. There are three kinds of extraction methods: distilled water, 50% EtOH and 100% EtOH. The condition of HPLC was obtained on a reversed-phase column $(Polarity\;dC_{18},\;4.6{\times}250 mm,\;5\;{\mu}m)$ using a phosphate buffer-acetonitrile-sodium lauryl sulfate as the mobile phase. Under these chromatographic conditions, UV detector was 230 nm, column temperature $30^{\circ}C$ and the speed of a current 1.0 ml/min, respectively. The results of extraction with distilled water, 50% EtOH and 100% EtOH in Angelica gigas roots were as follows. The concentrations of decursin and decursinol angelate were 182 and 153 ppm (distilled water), 3,142 and 2,547 ppm (50% EtOH) and 3,341 and 2,778 ppm (100% EtOH). There were high positive correlations between the concentrations of decursin and EtOH (r=0.8928, p<0.01) and decursinol angelate and EtOH (r=0.9009, p<0.01).