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HPLC Analysis and Extraction Methods of Decursin and Decursinol Angelate in Angelica gigas Roots  

Lee, Jang-Hoon (Dept. of Environmental Engineering, Hoseo University)
Chae, Hee-Jeong (College of Natural Science, Hoseo University)
Kim, Dong-Hyun (Dept. of Occupational Medicine, Dankook University)
Lee, Sang-Hyun (Natural Products Research Institute, Seoul National University)
Park, Sang-Yong (Dept. of Natural Medicines Evaluation, Korea Food & Drug Administration)
Kang, Young-Goo (Dept. of Industrial Safety Engineering, Hoseo University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.3, 2003 , pp. 201-205 More about this Journal
Abstract
This paper is intended as an investigation of the analysis of high-performance liquid chromatography and the method of extraction of decursin and decursinol angelate in Angelica gigas roots. There are three kinds of extraction methods: distilled water, 50% EtOH and 100% EtOH. The condition of HPLC was obtained on a reversed-phase column $(Polarity\;dC_{18},\;4.6{\times}250 mm,\;5\;{\mu}m)$ using a phosphate buffer-acetonitrile-sodium lauryl sulfate as the mobile phase. Under these chromatographic conditions, UV detector was 230 nm, column temperature $30^{\circ}C$ and the speed of a current 1.0 ml/min, respectively. The results of extraction with distilled water, 50% EtOH and 100% EtOH in Angelica gigas roots were as follows. The concentrations of decursin and decursinol angelate were 182 and 153 ppm (distilled water), 3,142 and 2,547 ppm (50% EtOH) and 3,341 and 2,778 ppm (100% EtOH). There were high positive correlations between the concentrations of decursin and EtOH (r=0.8928, p<0.01) and decursinol angelate and EtOH (r=0.9009, p<0.01).
Keywords
Angelica gigas; Umbelliferae; Decursin; Decursinol angelate; HPLC;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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