• Journal of Life Science
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• v.20 no.5
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• pp.750-759
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• 2010

The bioactive materials (phenolic compounds, flavonoids, minerals, decursin and decursinol angelate) and biological activities (DPPH [$\alpha,\alpha$'-diphenyl-$\beta$-picrylhydrazyl] free radical scavenging capability, reducing power, and tyrosinase activity) in the extracts of leaf, stem mixture (AGLS), and root (AGR) from Angelica gigas Nakai were examined by using water, hot water and ethanol solvent. The highest extract yield (21.89%) was found in the water extract of AGR. The highest concentrations of phenolic compounds and flavonoids in the ethanol extracts of AGLS and AGR were 14.99% and 14.79%. Major minerals of AGLS and AGR were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas, detected at 18.71 and 18.89 min of retention time by HPLC analysis, respectively. The highest concentrations of decursin and decursinol angelate in the Angelica gigas ethanol extract were found in root ($41.7\;{\mu}g/g$) and leaf ($34.04\;{\mu}g/g$). The highest free radical scavenging activity was found in the hot water extracts of AGLS and AGR, and its activity was stronger in all extracts of AGLS than AGR. The highest reducing power was found in the ethanol extracts of AGLS and AGR and this was dependent on the sample concentration. The hot water extracts of AGLS and AGR revealed the highest inhibition activity on tyrosinase. Overall, these results may provide the basic data needed to understand the biological activities of bioactive materials derived from Angelica gigas.

• Korean Journal of Pharmacognosy
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• v.19 no.4
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• pp.233-238
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• 1988

Angelica flaccida Kom. is a herbal plant growing wild in the marsh of valley in Korea. It has been used for sedative and analgesic as a folk medicine, but its constituents have not been clarified yet. Five known coumarins such as isoimperatorin, decursidin, (-) anomalin, decursin, decursinol and two sterols such as ${\beta}-sitosterol$ and stigmasterol were isolated from the root of this plant, Their structures were identified by the UV, IR, NMR, mass data and the physico-chemical properties.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.311-318
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• 1994

From the root of Angelica decursiva-albiflora Yook, which has been used as a folk medicine for a sedative, analgesic and expectorant, four free coumarins, e.g., decursidin(I), decursin(II), umbelliferone(III) and nodakenetin(IV), and two coumarin glycosides, e.g., nodakenin(V) and decuroside I(VI) were isolate. The cytotoxicity of nodakenin(V) against L-1210 leukemia cells was less effective than cisplatin, but in the nephrotoxicity against rabbit kidney proximal tubular cell nodakenin(V) showed remarkably less nephrotoxicant than cisplatin.

• YAKHAK HOEJI
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• v.30 no.2
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• pp.73-78
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• 1986

Peucedanum terebinthaceum Fischer (Umbelliferae) known as "Gi Reum Na Mool" in Korea and used for the treatment of cough, phlegm, headache and common cold etc. The substances were isolated from the fruits of Peucedanum terebinthaceum and identified by UV, GLC, IR, NMR, Mass spectra and physico-chemical tests. The steroidal compounds from the fruits were identified as $\beta$-sitosterol (tR 33.01 min.) and stigmasterol (tR 28.87 min.) by GLC. Substance I (colorless prismatic crystal, $C_{19}H_{20}O_5$, mp $110~111^{\circ}$) was identified as decursin which is one of the pyranocoumarin. Hydrolysis of substance I with 5%-potassium hydroxide produced decursinol. Acetylation of decursinot gave its monoacetate, white needles, $C_{16}H_{16}O_5$. Substance II(light yellowish white needle crystal, $C_9H_6O_3$, mp $227~228^{\circ}$) was proved to be umbelliferone. Substance I, decursin, was isolated for the first time from the fruits of Peucedanum spp. (Umbelliferae).

• Archives of Pharmacal Research
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• v.26 no.6
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• pp.449-452
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• 2003

Systematic fractionation of Angelica gigas roots led to the isolation of linear furano(pyrano)coumarins such as bergapten (1), decursinol angelate (2), decursin (3), nodakenetin (4) and nodakenin (5). The antibacterial activities of those compounds against pathogenic bacteria were investigated. Among the compounds tested, decursinol angelate (2) and decursin (3) exhibited significant antibacterial activity against Bacillus subtilis with the minimum inhibitory concentrations (MICs) of 50 and $12.5{\;}{\mu}g/mL$, respectively.

• Korean Journal of Pharmacognosy
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• v.11 no.1
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• pp.11-14
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• 1980

A Study of the effects of the root components of Angelica gigas Nakai (Umbelliferae) on voluntary activity in mice has confirmed that there are: 1) MeOH soluble extract, MeOH insoluble extract, decursin and decursinol were antagonized against the voluntary activity in mice pretreated with a dose of caffeine natrium benzoate 30mg/kg. 2) MeOH insoluble extract showed higher depressant effect than MeOH soluble extract. 3) The depressant activity of decursinol was significantly more highly effective than that of decursin.

• Archives of Pharmacal Research
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• v.26 no.9
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• pp.723-726
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• 2003

Five coumarins, isoimperatorin (1), pabulenol (2), isooxypeucedanin (3), oxypeucedanin hydrate (4) and osthol (5) were isolated from the MeOH extract of Angelica genuflexa in the course of searching for anti-platelet and anti-coagulant components from plants. Pabulenol (2) was isolated from A. genuflexa for the first time. The five compounds isolated from A. genuflexa, together with decursinol angelate (6), decursin (7) and nodakenin (8) from A. gigas were evaluated for their effects on platelet aggregation and blood coagulation. Compounds 2, 5, 6 and 7 were observed to be either equally effective or 2∼4 times more inhibitory than ASA in both arachidonic acid and U46619 ($TXA_2$ mimetic) induced platelet aggregations.

• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.197-201
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• 1996

To greatly decrease bolting rate and deterioration of root quality, seeds of Angelica gigas had sown and grown several month earlier than traditional seedtime. Roots were harvested in the middle of November in same year. Roots were devided to several parts such a fune root, secondary root, stele and cortex of main root. We ditermined and compared with the major constisuents contained in each parts of 1 and 2 year old roots. Among each parts of root, the amount of constituents in the fine root and cortex were much higher than those in stele of main root. Content of decursin and decursinol angelate was higher 6.5% in 2 year old root than 4.8% in 1 year old root and their component ratio showed 10:7.5 in 1 year old root and 10:6.0 in 2 year old root, respectively. But merits as a material of oriental medicine for 1 year old root that cultivated by early short-time growing seedling were generally acknowledged.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.1-8
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• 1995

This study was carried out to investigate the effects of floral inhibition of An-gelica gigas NAKAI in the hilly altitude located in the Northern Gyeongbuk Province from Feb. 1992 to Nov. 1994. The results obtained were as follows ： As the cultivated areas are high, rate of bolting was significantly decreased, having high yield, good growth, and medicinal quality. It is considered that the optimal cultivating area was at least above 600m altitude. In the hilly altitude, the more shorten nursery period was, the more decreased rate of bolting was, it results in decreased yield, having no significant differences in contents such as extracts and decursin. In bolting response from temperature treatment of the seedlings, treatment of high temperature was significantly decreased floral induction, but rate of establishment was decreased by decayed root. Bolting rate at different organic resources has more reduced in single fertilization than that of in organic application, but among organic resources, compost of rice straw has the lowest bolting rate. As a result, yield and medicinal qualities at various organic resources were increased in application of organic resources which was no con-siderable tendency among organic resources.

• YAKHAK HOEJI
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• v.50 no.3
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• pp.172-176
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• 2006

The purpose of this study is to develop a mass preparation method of (+)-decursinol from Angelica gigas roots. Recently; it has been shown that (+)-decursin, (+)-decursinol angelate and its analogues, isolated from Angelica gigas roots, exhibit various biological activities such as antitumor, antibacterial and neuroprotective activities. The contents of these compounds, ester form of (+)-decursinol, is very high in the Angelica gigas roots, whereas the content of (+)-decursinol itself is very low Therefore, (+)-decursinol which can be used as starting material to synthesize various its analogues was easily prepared from decursin analogues in the Angelica gigas roots. In order to achieve such aim, the Ether-Fr. of the ethanol exact of Angelica gigas roots was hydrolysed with various alkalis and solvents. As a result, the order of (+)-decursinol preparation was 1) NaOH, KOH, 2) $K_2CO_3$, and 3) $NaHCO_3$ as alkali. Also, the yield of (+)-decursinol was higher in diethyl ether than any other solvent conditions. From 1 kg of dried Angelica gigas roots, we could obtain 27.4 g of (+)-decursinol as a pure white solid.