Background: A great number of studies have shown that cytochrome P450 1A1 (CYP1A1) genetic polymorphisms, CYP1A1 Msp I and CYP1A1 Ile/Val, might be risk factors for digestive tract cancers, including esophageal cancer (EC), gastric cancer (GC), hepatic carcinoma (HC), as well as colorectal cancer (CC), but the results are controversial. In this study, a meta-analysis of this literature aimed to clarify associations of CYP1A1 genetic polymorphisms with digestive tract cancers susceptibility in Chinese populations. Materials and Methods: Eligible case-control studies published until December 2013 were retrieved by systematic literature searches from PubMed, Embase, CBM, CNKI and other Chinese databases by two investigators independently. The associated literature was acquired through deliberate search and selection based on established inclusion criteria. Fixed-effects or random-effects models were used to estimate odds ratios (ORs and 95%CIs). The meta-analysis was conducted using Review Manager 5.2 and Stata 12.0 softwares with stability evaluated by both stratified and sensitivity analyses. Moreover, sensitivity analysis and publication bias diagnostics confirmed the reliability and stability. Results: Eighteen case-control studies with 1,747 cases and 2,923 controls were selected for CYP1A1 MspI polymorphisms, and twenty case-control studies with 3, 790 cases and 4, 907 controls for the CYP1A1 Ile/Val polymorphisms. Correlation associations between CYP1A1 Ile/Val polymorphisms and digestive tract cancers susceptibility were observed in four genetic models in the meta-analysis (GG vs AA:OR= 2.03, 95%CI =1.52- 2.72; AG vs AA: OR=1.26, 95%CI =1.07-1.48; [GG+AG vs AA] :OR =1.42, 95%CI=1.20-1.68, [GG vs AA+AG]:OR=1.80, 95%CI =1.40-2.31). There was no association between CYP1A1 Msp I polymorphisms and digestive tract cancers risk. Subgroup analysis for tumor type showed a significant association of CYP1A1 Ile/Val genetic polymorphisms with EC in China. However, available data collected by the study failed to reveal remarkable associations of GC or HC with CYP1A1 Ile/Val genetic polymorphisms and EC, GC or CC with CYP1A1 MspI genetic polymorphisms. Conclusions: Our results indicated that CYP1A1 Ile/Val genetic polymorphisms, but not CYP1A1 Msp I polymorphisms, are associated with an increased digestive tract cancers risk in Chinese populations. Additional well-designed studies, with larger sample size, focusing on different ethnicities and cancer types are now warranted to validate this finding.
Journal of the Korea Academia-Industrial cooperation Society
/
v.7
no.6
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pp.1313-1318
/
2006
The mechanism fur the surface film formation was studied by in situ Atomic Force Microscopy (AFM) observation of a highly oriented pyrolytic graphite (HOPG) basal plane surface during cyclic voltammetry at a slow scan-rate of 0.5 mV $s^{-1}$ in 1 moi $dm^{-3}$ (M) $LiPF_6$ dissolved in a mixture of ethylene carbonate (EC) and diethyl carbonate (DEC). Decomposition of the electrolyte solution began at a potential around 2.15 V vs. $Li^+$/Li on step edges. In the potential range 0.95-0.8 V vs. $Li^+$/Li, flat areas (hill-like structures) and large swelling appeared on the surface. It is considered that these two features were formed by the intercalation of solvated lithium ions and their decomposition beneath the surface, respectively. At potentials more negative than 0.80 V vs. $Li^+$/Li, particle-like precipitates appeared on the basal plane surface. After the first cycle, the thickness of the precipitate layer was 30 nm. The precipitates were considered to be decomposition of the lithium salt ($LiPF_6$) and solvent molecules (EC and DEC), and to have an important role in suppressing further solvent decomposition on the basal plane.
The study of carbonaceous aerosols in the atmosphere is critical to understand the role of aerosols in human health and climate. Using standardized thermal optical transmittance methods, organic carbon (OC), elemental carbon (EC), and water soluble organic carbon (WSOC) were determined using a combustion sampling system for four types of agricultural crop residues (rice straw, red pepper stems, soybean stems, and green perilla stems) and eight types of forest trees (pine stems, pine needles, ginkgo stems, ginkgo leaves, maple stems, maple leaves, cherry stems, and cherry leaves). The aerosol particles between 0.056 and $5.6{\mu}m$ in size were analyzed using a Micro-Orifice Uniform Deposit Impactor (MOUDI). In the current study, the Carbonaceous Thermal Distribution (CTD) by carbon analyzer was discussed in order to understand the carbon fractions from the twelve types of biomass burning. Also, the concentration of OC, EC, WSOC, and water insoluble organic carbon (WIOC) detected in the emissions were described.
Journal of the Korean Society of Environmental Restoration Technology
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v.14
no.6
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pp.87-95
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2011
This study investigates Youl Chon Industrial Complex 1 by dividing into three regions : the drainage site, the area inside the complex, and the seaside. Then each region was subdivided into three areas resulting in a total of nine areas for soil sampling and analysis. After analyzing the physical and chemical properties of the soil, all nine area's pH and EC readings indicated that the soil condition is not suitable for vegetation growth (according to the previous study, the pH level was 5.8 and EC was 2.0dS/m). Therefore, for Youl Chon Industrial Complex 1 to become green, the neutralization and reduction of pH and EC level is needed; first, vegetation through dredging soil, and second, introduction of halophytes is suggested. This study finds out that vertical changes in physical and chemical properties of soils in reclaimed sites are very variable and the result depends on surrounding environments, so it suggested the basic data for building vegetation or reclaimed sites. However, the additional study aiming at various reclaimed sites is required as this study was conducted only to limited areas, and this study has left monitoring analysis as the follow-up task to check the relations between a plant community and soils.
To evaluate the effect of the fertilizer concentration after flowering on growth a31d fruit setting of ornamental pepper (Capsicum annuum L.), plants were fertilized with $100\;mg{\cdot}L^{-1} of N ($EC=0.8\;dS{\cdot}m^{-1}) until flowering, and then with 0 (no fertilizer), 100, 200 or $300\;mg{\cdot}L^{-1} of N (fertilizer solution EC of 0.15, 0.8, 1.45 or $2.10\;dS{\cdot}m^{-1}, respectively) until harvest. Maximum leaf area and shoot dry mass at the end of the growing period were obtained when plants were fertilized with $200\;mg{\cdot}L^{-1} of N. Total fruit number per plant at the end of the growing period was not different when plants were fertilized with 100,200 or 300 mg{\cdot}L^{-1}of N concentration. When plants were fertilized with $200\;mg{\cdot}L^{-1} of N, the number of fruits per plant was decreased significantly as compared to 100, 200 or $300\;mg{\cdot}L^{-1} of N, whereas the percentage of red fruits at the end of the growing period was maximized. Total fruit fresh weight per plant at the end of the growing period was highest with the concentration of $200\;mg{\cdot}L^{-1} of N. The EC of the growing medium remained within 0.8 to $1.2\;dS{\cdot}m^{-1}\;2.0\;to\;3.0dS{\cdot}m^{-1}, or 3.0 to 4.5 dS{\cdot}m^{-1}when fertilizer concentrations were 100, 200 or $300\;mg{\cdot}L^{-1} of N, respectively. Throughout most of the experiment, the pH of the growing medium remained within 5.4 to 6.2, but dropped to 4.9 near the end of the experiment when fertilizer concentration was 200 or 300\;mg{\cdot}L^{-1} of N. Content of most of the nutrients In the leaf was not affected by the different fertilizer concentration. Only aluminum was significantly affected and decreased linearly with increasing fertilizer concentration. The results from this study indicated that optimal fertilizer concentration after flowering for commercial production of ornamental pepper was 100 or $200\;mg{\cdot}L^{-1} of N. At these concentrations, the EC of the growing medium remained approximately within 0.8 to 1.2 and 2 to $3\;dS{\cdot}m^{-1}, respectively. This appears to be the optimal range for vegetative growth or fruit setting of ornamental pepper plants, and indicates that ornamental pepper can be grown with a fairly wide range of fertilizer concentrations.
Kim, Dong-Ho;Jang, Han-Sub;Choi, Gu-Il;Kim, Hyun-Jung;Kim, Ho-Jin;Kim, Hyo-Lin;Kim, Keun-Sung
Journal of Food Hygiene and Safety
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v.28
no.1
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pp.63-68
/
2013
Ethyl carbamate (EC) is a contaminant generated in the fermentation processes of various fermented foods. In this study, residue levels of EC in 95 alcoholic beverage samples were determined by using Gas Chromatography/Tandem Mass Spectrometry (GC/MS/MS). All the samples were purified by a liquid-liquid extraction (LLE) method using dichloromethane. The LLE method enables an improvement in time and cost to detection and specificity over the conventional extraction methods. The limits of detection and quantification (LOD and LOQ) to analyze EC were 1.3 and 4.0 ng/mL, respectively. The recovery rates of EC were ranged from 90.0 to 97.5% at the levels of 50, 100, and 500 ug/L. Among traditional grain-based alcoholic beverage samples (n = 34), the average residue levels of EC in takju, yakju, and cheongju were 0.63, 7.01, and 14.11 ug/L, respectively. Among fruit-based alcoholic beverage samples (n = 48), those of EC in japanese apricot spirits, bokbunjaju, grape wines, and other fruit wines were 79.18, 1.66, 2.64, and 2.39 ug/L, respectively. Among distilled or diluted alcoholic beverage samples (n = 13), those of EC in soju (distilled or diluted), general distillates, liquors, and brandies were 0, 3.30, 8.20, and 8.52 ug/L, respectively. Therefore, this study reports that the residue levels of EC in the alcoholic beverages, distributed in the current domestic markets, did not reach its maximum allowed levels of 30 and 400 ug/L established for grape and fruit wines in Canada, respectively.
This study was conducted to investigate the effect of the mixture ratio of the inorganic soil amendments on the soil physicochemical properties. In this experiment, three kinds of soil amendments which had similar pH, EC and particle size, the A, B and C, were tested. The mixture ratio of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA(United State of Golf Association) particle standard. To analyze the effects of amendment on chemical soil properties, pH, EC(electrical conductivity) and CEC(cation exchangeable capacity) were measured. The porosity, bulk density and hydraulic conductivity also measured to analyze the changes of physical properties. In the chemical properties, pH was significantly related to the mixture ratios of amendments, A and C(P<0.05), CEC and EC also related to the ratios of C(P<0.01). When the results were applied to the USGA standard of the soil physical properties, the optimum mixture ratios of each amendment were 3% in A and B, and 7~10% in C. To analyze the corelation of mixture ratio versus to physical character, volume of porosity was significantly related to the ratio of B (P<0.05), and showed similar corelation in porosity and hydraulic conductivity with ratio of C(P<0.05). These results indicate that types and mixture ratio of inorganic soil amendments should affect on soil physio-chemical properties of root zone on USGA sand green.
Background: To investigate the relationship of five TP53 polymorphisms (p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a) with the esophageal cancer (EC) risk in North Indians. Materials and Methods: Genotyping of p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a polymorphisms of TP53 in 136 sporadic EC patients and 136 controls using polymerase chain reaction and PCR-RFLP. Results: The frequencies of genotype RR, RP and PP of p.R72P polymorphism were 16.91 vs 26.47%, 58.82 vs 49.27% and 24.27 vs 24.27% among patients and controls respectively. We observed significantly increased frequency of RP genotype in cases as compared to controls (OR=1.87, 95% CI, 1.01-3.46, p=0.05). The frequencies of genotype A1A1, A1A2 and A2A2 of PIN3 ins16bp polymorphism were 69.12 vs 70.59%, 27.20 vs 25% and 3.68 vs 4.41% among patients and controls. There was no significant difference among genotype and allele distribution between patients and controls. The frequencies of genotype GG, GA and AA of r.13494g>a polymorphism were 62.50 vs 64.70%, 34.56 vs 30.15% and 2.94 vs 5.15% among patients and controls respectively. No significant difference between genotype and allele frequency was observed in the patients and controls. For p.P47S and p.R213R polymorphisms, all the cases and controls had homozygous wild type genotype. The RP-A1A1-GG genotype combination shows significant risk for EC (OR=2.01, 95%CI: 1.01-3.99, p=0.05). Conclusions: Among the five TP53 polymorphisms investigated, only p.R72P polymorphism may contributes to EC susceptibility.
It was conducted to identify all 47 isolates obtained from infected fruits of pepper and boxthorn, and to investigate the mycological characteristics and the response to fungicides. All of 11 isolates from pepper were identified as Colletotrichum acutatum included into A2 group. Among 36 isolates from boxthorn, 14 isolates were identified as C. gloeosporioides, and the others were done as C. acutatum, which were composed as A1 group with 15 isolates and A3 with 7 isolates. After incubating the isolates on PDA at $25^{\circ}C$ for 10 days, the colony color of C. acutatum was greyish white, while that of C. gloeosporioides was orange at center of colony and was gradually turned into an greyish white to the periphery. The rate of conidia showing ellongated ellipsoidal shape with round ends was over 95% in C. acutatum isolated from pepper. However, C. acutatum isolated from boxthorn produced ellongated ellipsoidal conidia with the rate of 75%, and the others were pointed at one or both ends. Regardless of species of Colletotrichum, all isolated used in this study was showed an optimal temperature at $25^{\circ}C$. $EC_{50}$ values of all isolates of Colletotrichum spp. to 2 fungicides as carbendazim and the mixture of carbendazim and diethofencarb was investigated by an agar dilution method. With C. acutatum isolates from pepper belonged to A2 group, the mean of $EC_{50}$ value to carbendazim and the mixture of carbendazim and diethofencarb was 0.68 and $3.16{\mu}g/ml$, respectively. In the case of C. acutatum isolates from boxthorn, which were divided into 2 groups as A1 and A3 group, that to carbendazim was 0.21 at A1 and $0.24{\mu}g/ml$ at A3, while that to the mixture was 1.52 and $3.35{\mu}g/ml$. Isolates of C. gloeosporioides showed the mean of $EC_{50}$ value was $0.12{\mu}g/ml$ to carbendazim and $0.92{\mu}g/ml$ to the mixture. The value of resistant factor was higher in the isolates of C. acutatum obtained in boxthorn than those from pepper.
Journal of the Korean Society of Food Science and Nutrition
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v.45
no.12
/
pp.1725-1731
/
2016
This study was carried out to investigate the effects of steam-drying on antioxidant compounds and antioxidant activity of Alliun hookeri root (AHR). The yield of methanolic extracts, total phenolic content (TPC), browning intensity (280 nm and 420 nm), and organosulfur compound contents (alliin and cycloalliin) in raw and steam-dried AHRs were 10.71~37.40%, $15.53{\sim}36.36{\mu}g/mg$, 0.48~2.09, 0.01~0.25, $1.46{\sim}700.61{\mu}g/mg$, and $1,173.95{\sim}2,182.60{\mu}g/mg$, respectively. Yield, TPC, and browning intensity of AHRs increased by steam-drying, whereas organosulfur compound contents decreased. Of all methanolic extracts from raw and steam-dried AHRs, four-time steam-drying showed the lowest $EC_{50}$ values (0.43, 7.53, 0.34, and 0.48 mg/mL, respectively) for DPPH radical scavenging activity, ABTS radical scavenging activity, SOD-like activity, and reducing power, whereas four-time steam-drying resulted in the highest TPC ($36.36{\mu}g/mg$) and browning intensity (2.09 and 0.25 at 280 and 420 nm, respectively). The antioxidant activities of methanolic extracts from raw and steam-dried AHRs were closely correlated with their TPC, browning intensity, and organosulfur compound content, showing correlation determination coefficient ($R^2$) values higher than 0.82. As a result, four-time steam-drying of AHRs could be useful as potential antioxidant sources.
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