• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.400-405
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• 1998

The ddh gene encoding meso-DAP-dehydrogenase (DDH) involved in the dehydrogenase pathway is essential for high-level lysine production in Brevibacterium lactofermentum. To investigate the effect of the ddh gene amplification on lysine production by B. lactofementum, we constructed two E. coli -B. lactofermentum shuttle vector, pEB1 and pEB2. The recombinant plasmids, pRK1 and pRK2, carrying the ddh gene were introduced into B. lactofermentum by electroporation. The specific activity of DDH by amplification of the ddh gene was increased 7-fold, and also L-Lysine production of B. lactofermentum strains harboring recombinant plasmids were 18∼20% higher than that of the control.

• International Journal of Concrete Structures and Materials
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• v.8 no.2
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• pp.117-128
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• 2014

Numerous investigations of RC beams strengthened in shear with externally-bonded (EB) fibre-reinforced polymer (FRP) sheets, plates and strips have been successfully conducted in recent years. These valuable studies have highlighted a number of influencing parameters that are not captured by the design guidelines. The objective of this study was: (1) to highlight experimentally and analytically the influential parameters on the shear contribution of FRP to RC beams strengthened in shear using EB FRP sheets and strips; and (2) to develop a set of transparent, coherent, and evolutionary design equations to calculate the shear resistance of RC beams strengthened in shear. In the experimental part of this study, 12 tests were performed on 4,520-mm-long T-beams. The specimens were strengthened in shear using carbon FRP (CFRP) strips and sheets. The test variables were: (1) the presence or absence of internal transverse-steel reinforcement; (2) use of FRP sheets versus FRP strips; and (3) the axial rigidity of the EB FRP reinforcement. In the analytical part of this study, new design equations were proposed to consider the effect of transverse-steel in addition to other influential parameters on the shear contribution of FRP. The accuracy of the proposed equations has been verified in this study by predicting the FRP shear contribution of experimentally tested RC beams.

• Korean Chemical Engineering Research
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• v.51 no.4
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• pp.476-481
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• 2013

Ethylbenzene (EB) which has a similar physical properties with p-xylene (pX) was separated from EB/pX mixture by using MFI-type zeolite (TS-1, ZSM-5, and Silicalite-1) coated membranes. The zeolites were synthesized by microwave method to reduce the synthesis time and uniformly formed zeolite particles were coated on the ${\alpha}$-almina tubular support with a thickness of $3-4{\mu}m$. Separation factor and permeation flux of the synthesized zeolite coated membranes were measured to survey the best performance of ethylbenzene separation from different composition of EB/pX mixtures. When the EB/pX mixture of 5:5 molar ratio applied for the separation experiment, it represented the highest separation factor. We also have studied about the effect of the atomic composition of zeolites on the separation performance within the temperature range from 160 to $220^{\circ}C$. TS-1 showed the highest permeation flux of $1,666mol/m^{2*}s^*Pa$ and Silicate-1 showed the highest separation factor of 1.73 at $200^{\circ}C$ respectively.

• Animal Bioscience
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• v.34 no.4
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• pp.533-538
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• 2021

Objective: Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegradable poly lactic-co-glycolic acid (PLGA) nanospheres in conjugation with growth factors (vascular endothelial growth factors [VEGF-A and VEGF-C]). Methods: We evaluated the practical use of heparin-conjugated PLGA nanoparticles (molecular weight ~15,000) in conjugation with VEGF-A/C, embryoid body (EB) formation, and LEC differentiation using immunofluorescence staining followed by quantification and quantitative real-time polymerase chain reaction analysis. Results: We showed that formation and differentiation of EB with VEGF-A/C-conjugated PLGA nanospheres, compared to direct supplementation of VEGF-A/C to the EB differentiation media, greatly improved yield of LYVE1(+) LECs. Our analyses revealed that the enhanced potential of LEC differentiation using VEGF-A/C-conjugated PLGA nanospheres was mediated by elevation of expression of the genes that are important for lymphatic vessel formation. Conclusion: Together, we not only established an improved protocol for LEC differentiation using PLGA nanospheres but also provided a platform technology for the mechanistic study of LEC development in mammals.

• Development and Reproduction
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• v.19 no.4
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• pp.217-226
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• 2015

A proper development of the epididymis during the early postnatal development is required for successful fertility in the adult male. Direct cell-cell communication via connexin (Cx) molecules is a common way of cellular interactions to achieve normal development of a given tissue consisting of different cell types. The present research was attempted to determine the effect of exogenous exposure to estrogenic agonist or antiandrogen at the weaning age on expression of Cx isoforms in the adult corpus epididymis. Male rats were subcutaneously administrated with estradiol benzoate (EB) or flutamide (Flu) at the weaning age. The tissue was collected at 4 months of age. Expressional levels of Cx isoforms were determined by a quantitative real-time PCR. Statistical comparison showed significant increases of Cxs31, 32, 37, 40, and 43 transcript amounts by a treatment of $0.015{\mu}g$ of EB /kg body weight (BW). A treatment of $1.5{\mu}g$ of EB /kg BW caused a significant decrease of Cx43 gene expression but increases of Cxs26, 31, 32, 37, and 40 transcript levels. Exposure to $500{\mu}g$ of Flu/kg BW induced an increase of Cx37 expression but significant decreases of Cxs43 and 45 mRNA levels. Expression of Cx37 was increased by a treatment of 5 mg of Flu/kg BW, while transcript levels of Cxs26, 30.3, 31, 31.1, 32, and 43 were significantly decreased by same treatment. These results demonstrate that exposure to steroidal compounds at the early developmental age alters expression of Cx isoforms in the adult corpus epididymis.

• Bulletin of the Korean Chemical Society
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• v.31 no.3
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• pp.611-614
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• 2010

Substituted imidazolidin-2-ones deduced as potential inhibitors of IleRS by docking simulations were synthesized from an aziridine-2-carboxaldehyde. Reductive amination of an aziridine-2-carboxaldehyde with dipeptides for the substituents at N1 and followed by aziridine-ring expansion with triphosgene afforded 4-chloromethylimidazolidin-2-ones whose chloride were further manipulated towards phenylurea, pyrimidin-2-yl-urea or benzenesulfonamide at C4.

• Analytical Science and Technology
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• v.19 no.4
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• pp.333-341
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• 2006

PVP-protected Ag, Pd and $Pt_{50}-Ru_{50}$ colloids were prepared independently by using ${\gamma}$-irradiation and electron beam (EB) at ambient temperature. UV-visible spectra of these colloids show the characteristic bands of surface resonance and give evidence for the formation of nanoparticles. Transmission electron microscopy (TEM) experiments were used to know the morphology of nanoparticles prepared by ${\gamma}$-irradiation and EB. The size of Ag, Pd, and $Pt_{50}-Ru_{50}$ nanoparticles prepared by ${\gamma}$-irradiation was ca. 13, 2-3, 15 nm, respectively. While, the size of Ag, Pd, and $Pt_{50}-Ru_{50}$ nanoparticles prepared by EB was ca. 10, 6, and 1-3 nm, respectively. Cyclic voltamograms (CV) were recorded for the Au electrodes immobilized with these nanoparticles. CVs indicated the modifications in the surface as a result of immobilization.

• The Journal of Society for e-Business Studies
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• v.10 no.4
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• pp.1-18
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• 2005

In ebXML, a representative framework for electronic commerce, a BPS (Business Process Specification) should be finally defined as an instance of XML-version BPSS for the configuration of B2B (Business to Business) runtime systems . In order to define the instance more complete and consistent, it is required to validate all the semantic constraints on the instance . Due to the limitations of XML Schema constructs, however, current XML-version BPSS fails to specify formal semantic constraints completely. This paper presents how to find, express and reuse BPSS semantic constraints that could not be explicitly defined in the XML-version BPSS. The method facilitates the validation of XML-version BPSS instances easily with some useful guides for fixing violations of semantic constraints. Furthermore, B2B business processes can be standardized and applied more efficiently and effectively.

• Journal of Astronomy and Space Sciences
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• v.14 no.1
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• pp.33-43
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• 1997

New BVR CCD photometric observations of EB type close binary V388 Cyg were obtained at the Sobaeksan Astronomy Observatory during 10 nights from October to December 1995. We analyse new 3 color (B, V & R) light curves simultaneously using the Wilson-Devinney code. Now, it is not yet clear whether V388 Cyg is contact or semidetached system. Published epochs of times of minima to data were used to study the changes in period of the system, and calculated a new light elements with the secular terms, $-4.83{\times}10^{-10}$, which indicated the continuous decrease of the period.

• Proceedings of the KAIS Fall Conference
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• 2011.05a
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• pp.323-326
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• 2011

HZSM-5, synthesized in fluoride medium, showed high selectivity for the formation of 1,4-diethylbenzene (1,4-DEB) in the vapour phase ethylation of ethylbenzene (EB) with ethanol and hence becomes a convenient eco-friendly substitute for hazardous mineral acid catalysts. De-ethylation of EB to benzene was also minimized over this catalyst. As the medium pore size and presence of weak and medium acid sites might be the cause of such benefits, fluoride mediated synthesis of ZSM-5 is proven to be advantageous for para-selective alkylation of alkyl aromatics.