• Title/Summary/Keyword: %24Y_H%24

Search Result 10,135, Processing Time 0.042 seconds

Effects of Exogenous Enzymes on Ruminal Fermentation and Degradability of Alfalfa Hay and Rice Straw

  • Yang, H.E.;Son, Y.S.;Beauchemin, K.A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.24 no.1
    • /
    • pp.56-64
    • /
    • 2011
  • This study was conducted to evaluate the use of exogenous enzymes as a potential means of improving the ruminal digestion (i.e., degradability) of alfalfa hay and rice straw. Twenty six enzyme-additives were examined in terms of protein concentration and enzymic activities on model substrates. The exogenous enzymes contained ranges of endoglucanase, xylanase, ${\beta}$-glucanase, ${\alpha}$-amylase, and protease activities. Six of the enzyme additives were chosen for further investigation. The enzyme additives and a control without enzyme were applied to mature quality alfalfa hay substrate and subsequently incubated in rumen batch cultures. Five of the enzyme additives (CE2, CE13, CE14, CE19, and CE24) increased total gas production (GP) at 48 h of incubation compared to the control (p<0.05). The two additives (CE14 and CE24) having the greatest positive effects on alfalfa hay dry matter, neutral detergent fibre (NDF) and acid detergent fibre (ADF) degradability were further characterized for their ability to enhance degradation of low quality forages. The treatments CE14, CE24, a 50:50 combination of CE14 and CE24 (CE14+24), and control (no enzyme) were applied to mature alfalfa hay and rice straw. For alfalfa hay, application of the two enzyme additives, alone and in combination, increased GP compared to the control at 48 h fermentation (p<0.05), whereas only CE14 and CE14+24 treatments improved GP from rice straw (p<0.05). Rumen fluid volatile fatty acid concentrations throughout the incubation of rice straw were analyzed. Acetate concentration was slightly lower (p<0.05) for CE14${\times}$CE24 compared to the control, although individually, CE14 and CE24 acetate concentrations were not different from the control. Increases (p<0.05) in alfalfa hay NDF degradability measured at 12 and 48 h of incubation occurred only for CE14 (at 12 h) and for CE14+24 (at 12 and 48 h). Similarly, ADF degradability increased (p<0.05) with CE14 and CE14+24. As for rice straw, increased DM degradability was observed at 12 and 48 h of incubation for all enzyme treatments with an exception for CE14 at 12 h. The degradability of NDF was improved by all the enzyme treatments at either incubation time, while ADF degradability was only enhanced at 48 h. Overall, the enzymes led to enhanced digestion of mature alfalfa and there was evidence of improved digestibility of rice straw, an even lower quality forage.

A study on the relationship between concentration of phosphorus, turbidity, and pH in water and soil (물과 토양에서 인의 농도, 탁도 그리고 pH와의 관계에 관한 연구)

  • Min, Young-Hong;Hyun, Dae-Yoeung;Eum, Chul-Hun;Lee, Seung-Ho
    • Analytical Science and Technology
    • /
    • v.24 no.4
    • /
    • pp.304-309
    • /
    • 2011
  • In this research, behaviour of turbidity and phosphorus in water and soil dependent upon pH and a change of water was studied. Phosphorus dissolve rate from turbidity was increased for water if potential of hydrogen was less than pH 4 or more than pH 7. Turbidity release rate from soil was increased with pH. Turbidity release rate from soil was drastically increased for water if potential of hydrogen was more than pH 4. turbidity release rate from soil was stabilized more than pH 6. Dissolved phosphorus was increased from 2 hours to 24 hours and stabilized in 24 hours. Turbidity was reached the peak of 24 hours and decreased from 24 hours to 96 hours. Turbidity and dissolved phosphorus was decreased for water if these samples were changed a overlying water. Behaviour of turbidity was analogous to dissolved phosphorus when potential of hydrogen was increased from pH 6 to pH 10 and a change of overlying water was increased from 1 time to 4 times. These results suggest that phosphorus dissolve rate and turbidity were directiy correlated with pH. These results are of great importance in lakes because most lakes have a pH in the range of pH 7-10.

Analysis of Nonclassical Fullerene C24 Regioisomers Encapsulating H2O using Hybrid Density Functional Methods B3LYP and M06-2X

  • Lee, Seol;Lee, Ji Young;Lee, Kee Hag
    • Bulletin of the Korean Chemical Society
    • /
    • v.35 no.3
    • /
    • pp.899-904
    • /
    • 2014
  • The atomic structures and electronic properties of six classical and nonclassical $H_2O$@$C_{24}$ fullerene regioisomers are systematically studied using the hybrid density functional B3LYP method and M06-2X method with empirical dispersion in conjunction with the 6-31G(d,p) basis sets. The charge transfer, frontier orbitals, dipole moment, energy gap between the HOMO and LUMO, and volume change of the $C_{24}$ cage are analyzed upon encapsulation of a $H_2O$ molecule in each $C_{24}$ regioisomer. All encapsulation processes are endothermic and the relative stabilities of six $C_{24}$ fullerene regioisomers change upon encapsulation of $H_2O$.

Analysis of Technology Convergence of 'Internet of Things' Patents by IPC Code Analysis (IPC 코드 분석에 의한 '사물인터넷(IoT)' 특허의 기술 융복합 분석)

  • Shim, Jae-ruen
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
    • /
    • v.9 no.3
    • /
    • pp.266-272
    • /
    • 2016
  • In this study, we analysed the technology convergence of Internet of Things(IoT) by International Patent Classification(IPC) code analysis. 163 patent applications in Korea are the subjects of this study. We confirmed that the most representative IPC code combinations between Main and Sub categories are G06Q 50/24-G06Q 50/22(6 cases), H04L 29/02-H04L 12/28(4 cases), G06F 15/16-G06F 3/048(3 cases), G06F 15/16-G06F 9/44(3 cases), and G06Q 50/22-G06Q 50/24(3 cases). The field of Health Care business have been prepared 9 patent applications by technology convergence between 'Health Care(G06Q 50/22)' and 'Patient Record Management(G06Q 50/24)'. Finally we also concluded that the core IPC code are G06F 15/16, G06Q 50/22, G06Q 50/24, and H04L 12/28 by the technology convergence interconnections analysis of IoT patent applications.

THE DEVELOPMENT OF INDWELLING WIRELESS PH TELEMETRY OF INTRAORAL ACIDITY (구강 내 산도의 생체 내 측정을 위한 wireless pH telemetry의 개발)

  • Kim, Hyung-Jun;Kim, Jae-Moon;Jeong, Tae-Sung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
    • /
    • v.35 no.1
    • /
    • pp.1-10
    • /
    • 2008
  • The purpose of this study was to develop the wireless pH telemetry lasting longer than 24 hours in the mouth to overcome the limits of conventional wire telemetry previously used for salivary and plaque pH measurement, and to assess its effectiveness. We developed a wireless pH telemeter which can measure and store the pH profile data during more than 24 hours. It was composed of intraoral part; pH sensor of antimony electrode, battery and microprocessor for data storage, and extraoral part; control/data receiver and data analyzing software which was newly made for this device. After inspecting wireless electrode for accurate measurement, it was attached to the removable intraoral appliance and delivered to the volunteer who was told to wear except brushing time, retrieved after 24 hours and finally the pH profile data was extracted and analyzed. When compared with conventional wire telemetry, this device showed similar results and induced less discomfort to examinees. The data showed pH changes at same time when examinees ate various scheduled foods and beverages. With this method it became possible to accurately measure pH changes within mouth for long time in accordance with individual's lifestyle, definitely reducing the discomfort inflicted to the examinees' life.

  • PDF

The Effects of Light on the Production of hGM-CSF in Transgenic Plant Cell Culture (빛 조사시간에 따른 형질전환된 담배세포 성장과 hGM-CSF의 생산에 미치는 영향)

  • 이재화;이재화;김영숙;홍신영;신윤지;서조은;권태호;양문식
    • KSBB Journal
    • /
    • v.16 no.6
    • /
    • pp.568-572
    • /
    • 2001
  • Light is one of the most important environmental factors controlling plant physiology. The human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was produced from cell suspension cultures of transgenic tobacco under different light conditions (24 hr light, 18 hr light/dark cycle, dark). Under 24 hr light condition, cell growth was best and dry cell weight reached 14.4 g/L. Light did not influenced the secretion of total proteins. However, in the dark condition, the ratio of secreted total protein/dry cell weight was 1.5 fold higher than those of ethel conditions. Production of hGM-CSF was highest with 18 hr light condition and reached 496.5 ug/L. In addition, the content of hGM-CSf in secreted total proteins was 1.8 fold higher than that of 24 hr light condition, which is beneficial for the purificationof the protein.

  • PDF

Study on Online Monitoring of Dissolved Oxygen, pH and Cell Concentration in E. coli Cultivation Processes Using MABOOMSTM (마이크로플레이트 기반 생물반응기 시스템 (MABOOMSTM)을 이용한 대장균 배양공정에서 용존산소, pH 및 세포농도의 온라인 모니터링 연구)

  • Sohn, Ok-Jae;Rhee, Jong Il
    • KSBB Journal
    • /
    • v.28 no.1
    • /
    • pp.24-30
    • /
    • 2013
  • Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.

The Relationship Between Esophageal Manometry and 24 Hour Double Prove pH-metry with Gastroesophageal Reflax in Pharyrngeal Neurosis Patients. (인두신경증 환자에서 Esophageal manometry와 24hour double prove pH metry 검사 및 위식도 역류와의 관계)

  • 김선태
    • Korean Journal of Bronchoesophagology
    • /
    • v.2 no.1
    • /
    • pp.88-96
    • /
    • 1996
  • In recently the gastroesophageal reflux disease(GERD) has been known to induce the otolaryngologic manifestations. Pharyngeal neurosis is a disease which we could have not found the cause frequently. So we have studied the relation between the pharyngeal neurosis and the GERD among 50 patients who were diagnosed as pharyngeal neurosis after esophagogram and laryngoscopic examination. We performed esophageal manometry and 24hour double-probe pH-metry and then compared with normal control group(n=30). The results are as follows 1 Among 50 patients, 12(24%) patients were diagnosed as GERD by DeMeester scoring. 2. In esophageal manometry, the upper and lower esophageal sphincter between the patients and the control group have no significant difference(p>0.05) and 9 among 50 pateints showed abnormal peristaltic movement in esophageal body contraction. 3. In 24hour double-probe pH-metry, the esophageal probe showed that in GERD group(n= 12) the number of reflux episode, episodes greater than 5 minutes and the percentage of time

  • PDF