• Korean Journal of Microbiology
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• v.38 no.2
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• pp.74-80
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• 2002

The purpose of this work was to investigate the characterization and sequence of catechol 1,2-dioxygenase (Cl,2O) purified from Acinetobacter sp. KS-1 which was grown on benzoate as a sole carbon source. Cl,2O demonstrated its enzyme activity to catechol and 4-methylcatechol. The optimum temperature of Cl,2O was $35^{\circ}C$, and the optimal pH was in the range from pH 7.5 to 9.0. $Ag^{+}$, $Hg^{+}$, and $Cu^{2+}$ showed inhibitory effect on the activity of Cl,2O. Molecular weight of the enzyme was determined to approximately 36 kDa by SDS-PAGE and 7-terminal amino acid sequence of Cl,2O was analyzed as $^{1}MNYQQIDALVKQMNVDTAKG^{20}$and exhibited 95% sequence homology with that of Cl,2O from Acinetobacter radioresistens In addition, trypsin digestion and peptide mapping were performed for internal sequencing analysis. Molecular weights of three digested peptide fragments were analyzed as 966.3 Da, 1933.8 Da and 2081.7 Da by MALDI-TOF, which were matched with each internal sequences $^{1}SQSDFNLRR^{9}\, ^{1}HGNRPSHVHYFNSAPGYR^{18}\, ^{1}TIEGPLYVAGAPESVGFAR^{19}$) of. A. radioresistens. PCR product was amplified with the degenerated primers derived from N-terminal and each internal amino acid sequences.

• The Plant Pathology Journal
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• v.34 no.6
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• pp.514-531
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• 2018

Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.370-375
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• 2016

Two xylanase genes were cloned into Escherichia coli from Bacillus sp. YB-1401 and B. amyloliquefaciens YB-1402, which had been isolated as mannanase producer from home-made doenjang, respectively, and their nucleotide sequences were determined. Both xylanase genes consisted of 642 nucleotides, encoding polypeptides of 213 amino acid residues. The deduced amino acid sequences of the YB-1401 and YB-1402 xylanase, designated Xyn1401 and Xyn1402, differed from each other by single amino acid residue, Asn for Xyn1401 and Lys for Xyn1402, corresponding to amino acid position of 127. Their amino acid sequences were highly homologous to those of xylanases belonging to the glycosyl hydrolase family 11. The 28 amino acid stretch in the N-terminus of both enzymes was predicted as signal peptide by SignalP4.1 server. Both xylanases were localized at the level of 91−94% in culture filtrate of the recombinant E. coli cells, suggesting they were secreted efficiently in E. coli cells. The optimal reaction conditions were 50℃ and pH 6.0 for Xyn1401, and 55℃ and pH 6.5 for Xyn1402, respectively, indicating one amino acid difference from each other affected pH and temperature profiles of their activities. In addition, their thermostabilities were somewhat different from each other.

• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.131-137
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• 1996

The sequences of p10 gene its promoter of Hyphantria cunea NPV were determined. According to the sequence analysis, the putative p10 gene ORF has 285 bp. The 5'-non-coding leader sequence of the p10 gene promoter contained the TATA box and the putative transcription initiation site TAAG motif. Poly (A) tail signals, AATAAA sequence was at site 65 base upstream from the 3' terminus. The deduced amino acid sequence of p10 protein was 95 with a predicted molecular weight of 10.26 kDa. In the p10 protein sequence, a hydrophobic region was present at the N-terminus of the protein, whereas the C-terminus was highly hydrophilic. The p10 protein of H. cunea NPV did not contain cysteine, histidine, trytophan, tryptophane, tyrosine, glutamine and asparagine residues.

• International Journal of Contents
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• v.10 no.4
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• pp.30-37
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• 2014

The current study was conducted to examine the temporal and spatial activation sequences related to morphosyntactic, semantic and orthographic-lexical sentences, focusing on the morphological-orthographic and lexical-semantic deviation processes in Korean language processing. The Event-related Potentials (ERPs) of 15 healthy students were adopted to explore the processing of head-final critical words in a sentential plausibility task. Specifically, it was examined whether the ERP-pattern to orthographic-lexical violation might show linear precedence over other processes, or the presence of additivity across combined processing components. For the morphosyntactic violation, fronto-central LAN followed by P600 was found, while semantic violation elicited N400, as expected. Activation of P600 was distributed in the left frontal and central sites, while N400 appeared even in frontal sites other than the centro-parietal areas. Most importantly, the orthographic-lexical violation process revealed by earlier N2 with fronto-central activity was shown to be complexes of morphological and semantic functions from the same critical word. The present study suggests that there is a linear precedence over the morphological deviation and its lexical semantic processing based on the immediate possibility of lexical information, followed by sentential semantics. Finally, late syntactic integration processes were completed, showing different topographic activation in order of importance of ongoing sentential information.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.7-10
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• 2001

Adsorption of the pesticides (phosphamidon, fenitrothion, triadimefon and diniconazole) in natural zeolite (CL $I_{N}$) and several synthetic zeolites was incestigated. The pesticides were not adsorbed on zeolites (Na-Pl, SOD, ANA, JBW and CAN) synthesized from Jeju scoria. The distribution coefficient ( $K_{D}$) and the Freundlich constant ( $K_{F}$) decreased in the following sequences. FC $C_{W}$ (waste catalytic cracking catalyst)>FA $U_{F}$ (FAU Synthesized from coal fly ash)>(FAU+Na-Pl)$_{SF}$ (the mixture of FAU and Na-Pl synthesized from the ratio of Jeju scoria 6 to coal fly ash 4 by weight)>CL $I_{N}$ among the zeolites; diniconazole>fenitrothion> triadimefon>phosphamidon. As the temperature was increased, the amount of pesticide adsorbed per unit mass of zeolite increased for FC $C_{W}$, FA $U_{F}$ and (FAU+Na-Pl)$_{SF}$ but it decreased for CL $I_{N}$, for all the pesticides used in this study. It was independent of pH for phosphamidon, fenitrothion and triadimefon, but decresed as pH was increased for all the zeolites used in this study.y.udy.y.y.y.y.y.y.

• Bulletin of the Korean Mathematical Society
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• v.58 no.3
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• pp.711-720
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• 2021

Let R denote a commutative noetherian ring, and let 𝐱 := x1, …, xd be an R-regular sequence. Suppose that 𝖆 denotes a monomial ideal with respect to 𝐱. The first purpose of this article is to show that 𝖆 is irreducible if and only if 𝖆 is a generalized-parametric ideal. Next, it is shown that, for any integer n ≥ 1, (x1, …, xd)n = ⋂P(f), where the intersection (irredundant) is taken over all monomials f = xe11 ⋯ xedd such that deg(f) = n - 1 and P(f) := (xe1+11, ⋯, xed+1d). The second main result of this paper shows that if 𝖖 := (𝐱) is a prime ideal of R which is contained in the Jacobson radical of R and R is 𝖖-adically complete, then 𝖆 is a parameter ideal if and only if 𝖆 is a monomial irreducible ideal and Rad(𝖆) = 𝖖. In addition, if a is generated by monomials m1, …, mr, then Rad(𝖆), the radical of a, is also monomial and Rad(𝖆) = (ω1, …, ωr), where ωi = rad(mi) for all i = 1, …, r.

• Journal of Ginseng Research
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• v.44 no.1
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• pp.135-144
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• 2020

Background: Panax species are important herbal medicinal plants in the Araliaceae family. Recently, we reported the complete chloroplast genomes and 45S nuclear ribosomal DNA sequences from seven Panax species, two (P. quinquefolius and P. trifolius) from North America and five (P. ginseng, P. notoginseng, P. japonicus, P. vietnamensis, and P. stipuleanatus) from Asia. Methods: We conducted phylogenetic analysis of these chloroplast sequences with 12 other Araliaceae species and comprehensive comparative analysis among the seven Panax whole chloroplast genomes. Results: We identified 1,128 single nucleotide polymorphisms (SNP) in coding gene sequences, distributed among 72 of the 79 protein-coding genes in the chloroplast genomes of the seven Panax species. The other seven genes (including psaJ, psbN, rpl23, psbF, psbL, rps18, and rps7) were identical among the Panax species. We also discovered that 12 large chloroplast genome fragments were transferred into the mitochondrial genome based on sharing of more than 90% sequence similarity. The total size of transferred fragments was 60,331 bp, corresponding to approximately 38.6% of chloroplast genome. We developed 18 SNP markers from the chloroplast genic coding sequence regions that were not similar to regions in the mitochondrial genome. These markers included two or three species-specific markers for each species and can be used to authenticate all the seven Panax species from the others. Conclusion: The comparative analysis of chloroplast genomes from seven Panax species elucidated their genetic diversity and evolutionary relationships, and 18 species-specific markers were able to discriminate among these species, thereby furthering efforts to protect the ginseng industry from economically motivated adulteration.

• Applied Microscopy
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• v.51
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• pp.17.1-17.10
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• 2021

Our previous study on the binding activity between Cel5H and clay minerals showed highest binding efficiency among other cellulase enzymes cloned. Here, based on previous studies, we hypothesized that the positive amino acids on the surface of Cel5H protein may play an important role in binding to clay surfaces. To examine this, protein sequences of Bacillus licheniformis Cel5H (BlCel5H) and Paenibacillus polymyxa Cel5A (PpCel5A) were analyzed and then selected amino acids were mutated. These mutated proteins were investigated for binding activity and force measurement via atomic force microscopy (AFM). A total of seven amino acids which are only present in BlCel5H but not in PpCel5A were selected for mutational studies and the positive residues which are present in both were omitted. Of the seven selected surface lysine residues, only three mutants K196A(M2), K54A(M3) and K157T(M4) showed 12%, 7% and 8% less clay mineral binding ability, respectively compared with wild-type. The probable reason why other mutants did not show altered binding efficiency might be due to relative location of amino acids on the protein surface. Meanwhile, measurement of adhesion forces on mica sheets showed a well-defined maximum at 69±19 pN for wild-type, 58±19 pN for M2, 53±19 pN for M3, and 49±19 pN for M4 proteins. Hence, our results demonstrated that relative location of surface amino acids of Cel5H protein especially positive charged amino acids are important in the process of clay mineral-protein binding interaction through electrostatic exchange of charges.

• Bulletin of the Korean Mathematical Society
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• v.47 no.3
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• pp.467-482
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• 2010

For a double array of random elements {$V_{mn};m{\geq}1,\;n{\geq}1$} in a real separable Banach space, some mean convergence theorems and weak laws of large numbers are established. For the mean convergence results, conditions are provided under which $k_{mn}^{-\frac{1}{r}}\sum{{u_m}\atop{i=1}}\sum{{u_n}\atop{i=1}}(V_{ij}-E(V_{ij}|F_{ij})){\rightarrow}0$ in $L_r$ (0 < r < 2). The weak law results provide conditions for $k_{mn}^{-\frac{1}{r}}\sum{{T_m}\atop{i=1}}\sum{{\tau}_n\atop{j=1}}(V_{ij}-E(V_{ij}|F_{ij})){\rightarrow}0$ in probability where {$T_m;m\;{\geq}1$} and {${\tau}_n;n\;{\geq}1$} are sequences of positive integer-valued random variables, {$k_{mn};m{{\geq}}1,\;n{\geq}1$} is an array of positive integers. The sharpness of the results is illustrated by examples.