• Journal of Microbiology and Biotechnology
• /
• v.33 no.11
• /
• pp.1531-1541
• /
• 2023

Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.

• Proceedings of the Korean Society of Organic Agriculture Conference
• /
• 2008.12a
• /
• pp.87-102
• /
• 2008

시설재배 토양에 녹비작물 재배와 고추재배, 휴경을 반복하면서, 외부 유기자재 투입을 최소화 하고 2년 동안 토양에 물리, 화학성에 변화를 추적하여 궁극적으로 녹비작물을 이용한 유기고추 시설 무경운 재배기술을 개발 하고자 시험을 수행한 결과 1. 고추 시설재배지 녹비작물 재배가 녹비 수량 및 토양 이화학성에 미치는 영향 시설재배지의 겨울 녹비작물을 1월 30일 파종하여 4월 5일에 조사한 결과 수량은 호밀과 보리녹비가 $518{\sim}677kg/10a$로 완두콩과 헤어리베치 $287{\sim}354kg/10a$에 비하여 건물수량이 현저하게 많았다. 녹비작물의 식물체내 총질소 함량이 완두콩과 헤어리베치, 보리가 호밀에 비하여 많았으며, 보리가 타 작물에 비하여 $P_2O_5$, $K_2O$ 함량이 높았고, 두과작물인 완두콩과 헤어리베치가 화본 과인 호밀과 보리녹비에 비하여 CaO과 MgO 함량이 많았다. 녹비작물의 토양중 무기성분 고정량이 화본과인 호밀과 보리녹비의 총질소량은 10a당 $17{\sim}18kg$, $P_2O_5$, 4.7kg, $K_2O$ $31{\sim}33kg$ 수준으로, 두과 녹비작물인 완두콩과 헤어리베치에 비하여 총질소, $P_2O_5$, 양이온인 $K_2O$ 높았으나, CaO과 MgO 고정량은 큰 차가 없었다. 2. 녹비작물 재배 후작 유기재배 고추의 생육 및 수량 겨울 재배 녹비작물을 트렉터로 로터리 작업하여 토양에 환원한 후 홍고추를 2007년 4월 27일 정식하여 조사한 결과 생육에 유의적인 차이를 보이지 않았으나, 고추 수량은 호밀녹비 재배지가 헤어리베치 재배지에 비하여 증수되는 경향을 보였다. 3. 시설재배지 2차 녹비작물 재배 시 녹비수량 및 토양 이화학성 2007년 12월 18일 2차 녹비작물을 파종 재배하여 생육량을 조사한 결과 건물 수량은 10a당 호밀은 720kg, 보리는 528kg, 헤어리베치는 230kg, 완두콩과 잡초는 217kg 수준이었다. 녹비작물 생체중의 일일 증가량은 호밀과 보리는 100kg/10a/day, 헤어리베치는 10a당 65kg 정도가 하루에 증가되었다. 녹비작물의 총질소 고정량은 화본인 보리와 호밀 녹비작물이 두과작물보다 건물수량이 많아서 2배정도 많았고, 인산은 $2.7{\sim}3.7$배, 가리의 고정량은 $2.2{\sim}2.6$배 정도 많았다. 4. 녹비작물 재배 후작 무경운 고추 유기재배의 생육 및 수량 2차 녹비작물 재배 토양에 무경운으로 풋고추를 2008년 4월 1일 정식하여 재배한 결과 녹비작물 종류에 따른 고추의 생육차는 인정되지 않았으나, 고추 수량은 무처리와 보리예취 이용 처리 및 녹비 생산량이 적은 완두콩 재배지에서 많았다. 5. 녹비작물과 고추 유기재배에 따른 시설토양 이화학성의 경시적 변화 비닐온실내의 토양에 무기화학성의 경시적 변화를 추적하여 본 결과 토양 pH는 녹비작물 재배 시 증가되는 경향을 보였으나, 고추재배 후에는 다시 토양 pH가 감소되었다. 토양에 EC는 녹비작물 재배 후 감소되었으나, 고추재배 후에는 다시 토양 EC농도가 증가되었다. 1차 녹비작물 재배 후에는 토양에 증가되었으나, 고추 재배 후에는 유기물함량이 감소되는 경향을 보였다. 토양에 총질소 함량은 녹비작물 2회 재배와 첫 번째 고추재배에서 감소되는 경향을 보였으나 두 번째 고추재배에서 급격한 증가를 보였다. 인산에 함량은 녹비작물재배와 고추 재배 시 미미하지만 공히 감소되는 경향을 보였다. 토양의 양이온 치환용량과 양이온인 토양 K, Ca, Mg함량은 녹비작물과 고추재배 시 감소되는 경향을 보였고, 고추재배에서는 같은 경향을 보였다.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.8
• /
• pp.1137-1143
• /
• 2015

This study investigated the free radical-scavenging and antitumor activities of hot water, water, acetone, ethanol, ethyl acetate, chloroform, and hexane extracts of fruits and leaves from Prunus mume. The various extracts were evaluated for their total polyphenol, flavonoid, and tannin contents, scavenging activities by DPPH and ABTS analyses, reducing power, protective effects against oxidative stress in L-132 cells, and antitumor activities against A549, HeLa, and U87 cancer cells. Ethanol extracts of fruits and leaves showed the highest total polyphenol content (336.41 and 523 mg GAE/100 g, respectively). DPPH and ABTS radical-scavenging activities increased according to concentration of fruit. DPPH radical-scavenging activity of ethanol extracts from leaves was 65.48% at $200{\mu}g/mL$. All extract fractions of leaves showed high ABTS radical-scavenging activities. The reducing power activities increased according to increasing concentration of fruits and leaves. All extracts of leaves performed better than extracts of fruits in terms of protective effects against oxidative stress in L-132 cells. Ethyl acetate, chloroform, hexane, ethanol extracts of fruits and leaves showed anticancer activities against A549, HeLa, and U87 cancer cells. However, ethanol extracts of fruits and leaves showed no toxicity in normal cells (BNLCL2). This study suggests that antioxidant activities of fruits and leaves from P. mume depend on polyphenol contents. Thus, fruits and leaves from P. mume can be useful as natural antioxidant compounds.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.47 no.6
• /
• pp.12-23
• /
• 2019

The purpose of this study was to develop and study an experiential natural education program that could emphasize the importance of the natural environment by providing natural experience opportunities using the natural resources of the urban forest parks using Youngheung Park in Suwon, Gyeonggi Province as a target site. The research target was limited to Suwon Yeongheung Park, which had the potential to become a place for education, where urban forest conservation and sustainable use already coexist. The natural education resources derived by surveying and analyzing the basic environment and the ecology of plants and animals in Suwon Yeongheung Park were organized to establish program goals, directions, and themes. Suwon Yeongheung Park is a water-rich forest that forms an ecological system of wetlands, including rice paddies, muknon wetlands, and dungbun, near a valley area. The U-shaped walkway was smoothly formed along the ridge and includes Doran-gil, which is among the Palochrome Road, designated by the city of Suwon. The soil is acidic, with a pH 4.40, due to urban pollution and acid rain, and is not good for plant growth. Most of the artificial forests, natural forests, and arable land were found using land use and extant life surveys. Old trees were distributed in artificial forests, the oak clusters in natural forests, and the fields and darrinones were distributed in the arable areas. As the forest vegetation declined, the cedar forest was underway, and the cedar trees and red bean pear trees were cultivated due to their adaptability to the urban environment. There are 13 large of 180 sacks, one being 109 centimeters in diameter, the largest silvery tree, and 105 oak trees, provide food and shelter for animals. Six species of waterfowl that used the 22 kinds of forest wetlands, while four species of amphibians and two species of reptiles reside in the wetlands. Natural Monument No. 327, Mauryuk, Class II Endangered Wildlife, was also observed in the wetlands. Eight other species of surface dragonflies and three species of butterflies were observed. By systemizing the resources, members, and characteristics of the forest ecosystems in Suwon Yeongheung Park based on five criteria, the program for a hands-on natural education was presented with the aim of understanding the urban forest ecosystem in Suwon Yeongheung Park, having an affinity with the city, and recognizing its relationship with the community and society. However, further research is needed as there are limitations of research on programs characterized by different ages and classes.

• Korean Journal of Organic Agriculture
• /
• v.13 no.4
• /
• pp.389-400
• /
• 2005

This study was conducted to investigate into the ecological environments and the soil microflora of purple-bracted plantain lily (Hosta longipes Matsumura) for wild vgetables. Native soil textures of purple-bracted plantain lily were in the order of sandy loam (SL) > loam (L) > clay loam (CL). pH in soil was relatively acid by 4.8, electric conductivity was 0.08mS/cm, and organic matter content was 0.08g/kg. CEC was measured by $100.8cmol^{(+)}kg^{-1}$ and available phosphate was 103.4mg/kg. Contents of exchangeable cations in terms of potassium, calcium, and magnesium were measured by $0.33cmol^{(+)}kg^{-1},\;2.26cmol^{(+)}kg^{-1},\;and\;0.87cmol^{(+)}kg^{-1}$, etc. Diurnal changes in the air temperature of the natives were 15 to $20^{\circ}C$, that temperature differential was relatively little compared with that in open field by 15 to $30^{\circ}C$. Relative humidity in the natives were much more humid by 60 to 80% compared with that in open feld by 35 to 85%. Light intensity in the natives and the open field at ten o'clock were $2,300{\mu}mol/m^2/sec.\;and\;1,750{\mu}mol/m^2/sec.$ Total number of soil microorganisms were $8.4{\times}10^7\;c.f.u./g$. Mycorrhizal spore densities over $500{\mu}m,\;355{\sim}500{\mu}m,\;251{\sim}354{\mu}m,\;107{\sim}250{\mu}m\;and\;45{\sim}106{\mu}m$ were 0.8, 1.3, 2.1, 38.1, and 110.0 respectively. Mycorrhizal root infections by vesicle and hyphae were 17% and 6%. However, arbuscules in the roots were not shown.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.28 no.4
• /
• pp.673-682
• /
• 2001

The use of resin composites has continued to increase over the last several years. In spite of their growing popularity, composites continue to exhibit a number of undesirable characteristics. One of the major deficiencies of composite restorative resins is their inadequate resistance to wear. Of the multitude of factors that have been associated with wear, subsurface degradation within the restoration is considered to be one. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. This solution with a high concentration of hydroxyl ions is a convenient medium for accelerated degradation of silane coupling and filler particles. The brands studies were Definite($Degussa-H\ddot{u}ls$ AG, Germany), Prodigy(Kerr, USA), Pyramid(Bisco, USA) and Synergy(Coltene, Swiss). Preweighed discs of each brand were exposed to 0.1N NaOH solution at $60^{\circ}C$. After 14 days they were removed, neutralized with HCl, washed with water and dried. Resistance to degradation was evaluated on the basis of following parameters : (a) mass loss(%)-determined from pre-and post-exposed specimen weights : (b) Si loss(ppm)-obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation $depth({\mu}m)$-measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows: 1. Mass loss of Synergy was $1.24{\pm}0.002%$, it was the highest, there was no significant difference among the materials. 2. The degree of degradation layer depth of Synergy was $107.83{\pm}2.52{\mu}m$, it was the highest, there was no significant difference among any other materials than Synergy. 3. There was no difference among the four materials in Si loss. 4. The correlation coefficient between mass loss and degradation depth was relatively high(r=0.06, p<0.05). 5. There was no coefficient correlation between Si loss and mass loss, the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler.

• Journal of Life Science
• /
• v.22 no.10
• /
• pp.1295-1306
• /
• 2012

A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater and identified as Bacillus atrophaeus. This species was designated as B. atrophaeus LBH-18 based on its evolutionary distance and the phylogenetic tree resulting from 16S rDNA sequencing and the neighbor-joining method. The optimal conditions for rice bran (68.1 g/l), peptone (9.1 g/l), and initial pH (7.0) of the medium for cell growth was determined by Design Expert Software based on the response surface method; conditions for production of CMCase were 55.2 g/l, 6.6 g/l, and 7.1, respectively. The optimal temperature for cell growth and the production of CMCase by B. atrophaeus LBH-18 was $30^{\circ}C$. The optimal conditions of agitation speed and aeration rate for cell growth in a 7-l bioreactor were 324 rpm and 0.9 vvm, respectively, whereas those for production of CMCase were 343 rpm and 0.6 vvm, respectively. The optimal inner pressure for cell growth and production of CMCase in a 100-l bioreactor was 0.06 MPa. Maximal production of CMCase under optimal conditions in a 100-l bioreactor was 127.5 U/ml, which was 1.32 times higher than that without an inner pressure. In this study, rice bran was developed as a carbon source for industrial scale production of CMCase by B. atrophaeus LBH-18. Reduced time for the production of CMCase from 7 to 10 days to 3 days by using a bacterial strain with submerged fermentation also resulted in increased productivity of CMCase and a decrease in its production cost.

• Journal of Chest Surgery
• /
• v.30 no.7
• /
• pp.677-685
• /
• 1997

Thromboelastography(TEG) is the unique measure that gives rapid information about the whole clotting process. Simplifying the diagnosis of coagulopathy during operations, TEG can provide an adequate therapy for postoperative bleeding. Remarkable improvement in hemostasis after cardiopulmonary bypass(CPB) has been achieved by the treatment with proteinase inhibitor aprotinin, but the hemostatic mechanism of aprotinin during CPB is still unclear. This study was designed to evaluate the effects of aprotinin on coagulation system during CPB by using TEG. Forty patients who underwent CPB were divided into two groups: aprotinin(2u 106 kallikrein inhibition units, as a single dose into the cardiopulmonary bypass priming solution) treatment group(male 14, female 8, mean age=50.Byears) and no aprotinin treatment(control) group(male 10, female 8, mean age=53.4 years). TEG, activated clotting time, prothrombin time, activated partial thromboplastin time, platelet counts, fibrinogen an (ibrinogen degradation product(FDP) concentrations were checked before and after CPB(30 minutes after neutralization of heparin effect by protamine sulfate). There was no significant difference in other conventional coagulation tests of two groups except postcardiopulmonary bypass FDP concentration in control group, which was significantly increased compared to that in aprotinin group(p<0.05). In TEG variables of both groups, clot formation time(K) and alpha $angle(\alpha^{\circ})$ were significantly increased and decreased, respectively, after CPB(p<0.05), but fibrinolytic index(LYS60) was not changed during CPB. In aprotinin group, reaction time(R) was decreased significantly after CPB(p<0.05) but maximum amplitude(MA) was not changed(p>0.05). On the contrary, R was not changed markedly but MA was decreased significantly in control group after CPB(p<0.05). This result shows that main change in coagulation system during CPB is not hyperfibrinolysis but cecrease in clot strength by platelet dys unction, and the main effect of aprotinin during cardiopulmonary bypass is the maintenance of clot strength to the pre-CPB level by the preservation of platelet function.

• Korean Journal of Soil Science and Fertilizer
• /
• v.12 no.3
• /
• pp.125-132
• /
• 1979

A field experiment was conducted to evaluate the effectiveness of an organic fertilizer (Glutamic acid fermentation by product ameded with nitrogen) on the growth and yield of radish and chinese cabbage on a relatively fertile soil. Additional laboratory studies were also made to find out the influence of mentioned fertilizer on the phisical and chemical characteristic of soils. The results are summarized as following. 1. Effect of fertilizer on the growth and yield. a. On a relatively fertile soil with high organic matter content (3%), the organic fertilizer was not effective for the growth and yield of radish and yield of radish and chinese cabbage. b. The application of organic fertilizer increased the K contents in plants both in radish and chinese cabbage. It was observed that the high content of K in radish leave lowered the leaf/root ratio and the leaf/root ratio showed a positive correlation with root weight. 2. Effect on soil physical and chemical properties. a. The application of organic fertilizer resulted in the improvement of soil physical property by lowering bulk density and increasing porosity. b. It was found that application of organic fertilizer increased the water stable soil aggregates. c. The application of organic fertilizer did not result in the significant increase in soil organic matter. d. Soil pH was lowered by the application of organic matter. It was speculated that the oxidation of ammonical nitrogen in the fertilizer is responsible for the lowering of soil pH.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.29 no.2
• /
• pp.87-97
• /
• 2023

In this study, we examined the effects of a large wildfire in the coastal area of Uljin-gun. The analysis of water quality and the dominant species, species composition, and community structure of marine algal was conducted quarterly in 2022 at Nagok (F-1), Hujeong (F-2), Bongpyeong (F-3), and Gongse Port (F-C) in Uljin-gun. As a result of water quality analysis, the pH, a factor of wildfire impact was 8.07~8.30 and 8.12~8.48 in surface and bottom layers, respectively, which are normal values in coastal waters of the East Sea, suggesting no direct impact from wildfires. By marine algal species composition, the coastal areas show the following order: Rhodophyta (58.1%) > Ochrophyta (25.8%) > Chlorophyta (14.5%) > Magnoliophyta (1.6%). By season, Undaria pinnatifida was the most dominant at Nagok and Hujeong in March and June, which in September and November, Gelidium elegans and Lithophyllum sp. were the most dominant in Bongpyeong and Gongse Port, respectively. In the cluster analysis, the stations were divided into two groups according to presence and absence of specific marine algal by season. The dominant species were U. pinnatifida, G. elegans and D. divaricata in group A, and Lithophyllum sp. was mainly present in group B. Thus, the species composition and group structure reflected the normal seasonal change pattern with water temperature variation and showed little significant difference from the control site, suggesting no direct effects of the wildfire on algae distribution in Uljin.