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A comparison of chlorhexidine release rate from three polymeric controlled release drug prototypes (제어방출형 소독제의 약물전달 체로 사용된 폴리머 유형에 따른 클로르헥시딘 제어 방출속도 비교)

  • Bok Young-Bin;Lee Doug-Youn;Lee Chang-Young;Kim Kyung-Nam;Kum Kee-Yeon
    • Restorative Dentistry and Endodontics
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    • v.29 no.6
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    • pp.548-552
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    • 2004
  • Intracanal disinfection of infected root canal is one of important treatment procedure. This in vitro study aimed to evaluate whether the surface polymers of controlled release drug (CRD) can effectively control the release rate of chlorhexidine for root canal disinfection. Four CRD prototypes were prepared: Group A (n=12); The core device (absorbent paper point) was loaded with 40% CHX solution as control. Group B (n=12); same as group A, but the device was coated with chitosan. Group C (n=12); same as group A and then coated three times with 5% PMMA. Group D (n=12); same as group A and then coated three times with 3% PLGA. All CRD prototypes were soaked in 3 mL distilled water for experimental periods and the concentrations of released CHX from each CRD prototype were determined using a UV spectrophotometer. Results showed that release rate of CHX were the greatest in the non-coated group (control group), followed by the chitosan-coated group, the PLGA-coated group, and the PMMA-coated group (P < 0.05). This data indicate that surface polymers can control the release rate of CHX from the CRD prototypes.

The Effect of Environmental Orientation on Innovation, Environmental and Market Performance in Franchise Firms Context (프랜차이즈기업의 환경지향성이 혁신, 환경성과와 시장성과에 미치는 영향)

  • Kim, Min-Seong;Seo, Min-Kyo;Park, Soon-Woo;Lee, Yong-Ki
    • Journal of Distribution Science
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    • v.12 no.8
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    • pp.71-82
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    • 2014
  • Purpose - This study is to examine the effect of environmental orientation on innovation, environmental and market performance in Korean franchise firms' context. This study also investigates how environmental orientation, innovation and environmental performance play mediating roles between environmental orientation and market performance. For these purposes, the authors developed a structural model which consists of several constructs. In the model, environmental orientation was proposed to affect innovation and environmental performance, while innovation and environmental performance influence market performance. Specifically, innovation and environmental performance were proposed as core mediators between franchise firms' environmental orientation and market performance. Research design, data, and methodology - The data were collected from 155 franchise firms in Korea during January 2012. The results of factor analysis with reliability test with Cronbach's α warranted unidimensionality of the measures for each construct. In addition, nomological validity of the measures of each construct was warranted from the result of correlation analysis. The data were analyzed with reliability analysis, and structural equation modeling with SPSS Win/PC 18.0 and AMOS 18.0. Results - The results of the overall model analysis appeared as follow: χ2=52.388(df=21), p=.000. GFI=.932, AGFI=.854, NFI=.964, CFI=.978, RMSEA=.099, RMR=.059. Since the result of the overall model analysis demonstrated a good fit, we further analyzed our data. The findings can be summarized as follows: Firstly, the environmental orientation had a positive effect on innovation and environmental performance, but had not a direct effect on market performance. Secondly, innovation had a positive effect on environmental performance, but had not a direct effect on market performance. Thirdly, environmental performance had a positive effect on market performance. Conclusions - The results of this study show that, in order to achieve competitive advantages and environmental and market performance in turbulent market situation, franchise firms should emphasize internal and external environmental orientation, and put through improvements in perspective of technology, organization, and product. Consequently, this research suggests that environmental orientation is a motive for franchise companies' innovation, environmental performance, and market performance. It also suggests theoretical and managerial implications, limitations, and further research directions.

Abdomino-perineal Organ Injuries Caused by Cultivators (경운기 사고에 의해 발생한 복부 및 회음부 장기 손상)

  • Yeo, Kwang Hee;Park, Chan Yong;Kim, Ho Hyun;Park, Soon Chang;Yeom, Seok Ran
    • Journal of Trauma and Injury
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    • v.28 no.2
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    • pp.60-66
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    • 2015
  • Purpose: Cultivator accidents are frequent and often lead to abdomino-perineal organ injury and, if severe, to death. This study presents the clinical characteristics, outcomes, and factors associated with mortality in patients who sustained an abdomino-perineal organ injury in cultivator accidents. Methods: We retrospectively analyzed the records of 53 patients who visited the emergency department of a tertiary hospital with abdomino-perineal organ injuries caused in cultivator accidents from April 2005 to March 2010. Results: All 53 patients had visited other medical institutions before visiting our hospital. Their mean age was $64.0{\pm}11.1$ (range, 20-80) years and 32 (60.4%) patients were 65 or older. The male-to-female ratio was 46:7. The chief complaint was abdominal pain (38 cases, 71.7%). The 53 patients included 41 cultivator operators (77.4%), 11 passengers (20.8%), and 1 passerby (1.9%). The causes of the injuries included a direct impact of the handlebar in 20 cases (37.7%), a rollover in 21 cases (39.6%), a fall in 10 cases (18.9%), and a wheel in two cases (3.8%). Several of the 53 patients had injuries to multiple abdomino-perineal organs, and the injured organs included the liver (23 cases, 26.4%), spleen (16 cases, 18.4%), pancreas (7 cases, 8.0%), small bowel (7 cases, 8.0%), mesentery (6 cases, 6.9%), adrenal gland (5 cases, 5.8%), and other organs. According to the abbreviated injury scale (AIS) dictionary, a thoracic injury was the most frequent co-injury (33 of 53 cases, 62.3%). Abdomino-perineal surgery was performed in 31 cases (58.8%) and angio-embolization was performed for six liver and two kidney injuries. Thirteen patients died (24.5%); all were males. The Injury Severity Scale (ISS) was lower in the survivors ($17.8{\pm}8.5$ vs. $27.0{\pm}16.0$; p=0.010). Conclusion: With the aging of agricultural workers, safety education programs should be implemented. Furthermore, the patient transfer system in agricultural areas must be improved.

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Forest Structure of Jisimdo Hallyeohaesang National Park (한려해상국립공원 지심도의 식생구조)

  • Ahn, Hyun-Chul;Kim, Jeong-Woon;Choo, Gab-Chul;Shin, Hyun-Soo;Park, Sam-Bong;An, Jong-Bin;Park, Jeong-Geun;Ngondya, Issakwisa Bernard
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.27-36
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    • 2012
  • The purpose of this study was to investigate the structure of the vegetation on the trail of Hallyeo Marine & Coastal National Park. Twenty six $10{\times}10m$ plots were established along the Jisimdo trail. The vascular plants were surveyed from March 2011 to October 2011. Camellia japonica was a major woody species in the study area, while Pinus thunbergii and Zanthoxylum ailanthoides were co-dominant tree species. There were positive correlations between Distylium racemosum and Ligustrum japonicum var. japonicum; Callicarpa japonica var. luxurians and Eurya japonical; Actinodaphne lancifolia and Cinnamomum japonicum; A. lancifolia and Machilus japonica. Positive correlations were found between A. lancifolia and P. thunbergii; Z. ailanthoides and C. japonicum. Species diversity (H') was ranged from 0.51907 to 1.0217, and was relatively low compared to those of same national parks. 10 populations of Milletia japonica which are rare and endemic species, were recorded from the list of rare and endemic species of the Ministry of Environment near by ammo dumps, this study recommend the long term habitat monitoring of the species.

Validation on the Analytical Method of Ginsenosides in Red Ginseng

  • Cho B. G.;Nho K. B.;Shon H. J.;Choi K. J.;Lee S. K.;Kim S. C;Ko S. R.;Xie P. S.;Yan Y. Z.;Yang J. W.
    • Proceedings of the Ginseng society Conference
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    • 2002.10a
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    • pp.491-501
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    • 2002
  • A cross-examination between KT&G Central Research Institute and Guangzhou Institute for Drug Control was carried out in order to select optimum conditions for extraction, separation and determination of ginsenosides in red ginseng and to propose a better method for the quantitative analysis of ginsenosides. The optimum extraction conditions of ginsenosides from red ginseng were as follows: the extraction solvent, $70\%$ methanol; the extraction temperature, $100^{\circ}C;$ the extraction time, 1 hour for once; and the repetition of extraction, twice. The optimum separation conditions of ginsenosides on the SepPak $C_{18}$ cartridge were as follows: the loaded amount, 0.4 g of methanol extract; the washing solvents, distilled water of 25 ml at first and then $30\%$ methanol of 25 ml; the elution solvent, $90\%$ methanol of 5 ml. The optimum HPLC conditions for the determination of ginsenosides were as follows: column, Lichrosorb $NH_2(25{\times}0.4cm,$ 5${\mu}m$, Merck Co.); mobile phase, a mixture of acetonitrile/water/isopropanol (80/5/15) and acetonitrile/water/isopropanol (80/20/15) with gradient system; and the detector, ELSD. On the basis of the optimum conditions a method for the quantitative analysis of ginsenosides were proposed and another cross-examination was carried out for the validation of the selected analytical method conditions. The coefficient of variances (CVs) on the contents of ginsenoside-$Rg_{1}$, -Re and $-Rb_1$ were lower than $3\%$ and the recovery rates of ginsenosides were $89.4\~95.7\%,$ which suggests that the above extraction and separation conditions may be reproducible and reasonable. For the selected HPLC/ELSD conditions, the CVs on the detector responses of ginsenoside-Rg, -Re and $-Rb_1$) were also lower than $3\%$, the regression coefficients for the calibration curves of ginsenosides were higher than 0.99 and two adjacent ginsenoside peaks were well separated, which suggests that the above HPLC/ELSD conditions may be good enough for the determination of ginsenosides.

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Understanding of a Korean Standard for the Analysis of Hexavalent Chromium in Soils and Interpretation of their Results (토양오염공정시험기준 6가크롬 분석의 이해와 결과 해석)

  • Kim, Rog-Young;Jung, Goo-Bok;Sung, Jwa-Kyung;Lee, Ju-Young;Jang, Byoung-Choon;Yun, Hong-Bae;Lee, Yee-Jin;Song, You-Seong;Kim, Won-Il;Lee, Jong-Sik;Ha, Sang-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.727-733
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    • 2011
  • A new Korean standard for the determination of Cr(VI) in soils has been officially published as ES 07408.1 in 2009. This analytical method is based on the hot alkaline digestion and colorimetric detection prescribed by U.S. EPA method 3060A and 7196A. The hot alkaline digestion accomplished using 0.28 M $Na_2CO_3$ and 0.5 M NaOH solution (pH 13.4) at $90{\sim}95^{\circ}C$ determines total Cr(VI) in soils extracting all forms of Cr(VI), including water-soluble, adsorbed, precipitated, and mineral-bound chromates. This aggressive alkaline digestion, however, proved to be problematic for certain soils which contain large amounts of soluble humic substances or active manganese oxides. Cr(III) could be oxidized to Cr(VI) by manganese oxides during the strong alkaline extraction, resulting in overestimation (positive error) of Cr(VI). In contrast, Cr(VI) reduction by dissolved humic matter or Fe(II) could occur during the neutralization and acidic colorimetric detection procedure, resulting in underestimation (negative error) of Cr(VI). Futhermore, dissolved humic matter hampered the colorimetric detection of Cr(VI) using UV/Vis spectrophotometer due to the strong coloration of the filtrate, resulting in overestimation (positive error) of Cr(VI). Without understanding the mechanisms of Cr(VI) and Cr(III) transformation during the analysis it could be difficult to operate the experiment in laboratory and to evaluate the Cr(VI) results. For this reason, in this paper we described the theoretical principles and limitations of Cr(VI) analysis and provided useful guidelines for laboratory work and Cr(VI) data analysis.

In Vitro Tissue Culture Frequency and Transformation of Various Cultivars of Soybean (Glycine max (L.) Merr.) (다양한 콩 자원들의 기내 조직배양 효율 및 형질전환)

  • Seo, Mi-Suk;Cho, Chuloh;Jeong, Namhee;Sung, Soon-Kee;Choi, Man-Soo;Jin, Mina;Kim, Dool-Yi
    • Korean Journal of Plant Resources
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    • v.34 no.4
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    • pp.278-286
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    • 2021
  • Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

A Study on the Minimum Engine Propulsion Power Required for Safe Navigation of Small and Medium Ships (중소형 선박의 안전항해를 위한 주기관 최소출력에 관한 연구)

  • Kang, Suk-Young;Ahn, Young-Joong;Kim, Seung-Yeon;Lee, Yun-Sok
    • Journal of Navigation and Port Research
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    • v.42 no.6
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    • pp.415-420
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    • 2018
  • The Energy Efficiency Design Index (EEDI) introduced by the Marine Environment P rotection Committee(MEPC) in International Maritime Organization(IMO) has significantly assisted in regulating CO2 emissions. However, in adverse weather conditions, it can lead to accidents due to slow steaming of vessels and low engine propulsion power. In response to this issue, the MEPC presented guidelines for the minimum propulsion power of the main engine for maintaining the course of vessels in adverse weather conditions. However, the guidelines are only applicable for vessels with a deadweight of 20,000 tons, leaving out small and medium ships. This study evaluated vessels subject to the guidelines of minimum propulsion power and proposed revised guidelines. In addition, relevant cases of marine accidents were investigated with the aim of investigating the minimum propulsion power of main engine for medium and small ships not covered by the guidelines. In order to achieve this, engine propulsion power was analyzed according to the size of the affected vessels. The results obtained from this study could be used as a minimum power criterion that can be considered for ship building to reduce marine accidents in adverse weather for small and medium ships.

Evaluation for Ultimate Flexural Strength of Steel Composite Girder with High Strength Concrete (고강도 콘크리트 강합성 거더의 극한휨강도 실험 평가)

  • Kim, Woon Hak;Lee, Juwon;Lee, Seokmin
    • Journal of the Society of Disaster Information
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    • v.16 no.4
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    • pp.796-805
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    • 2020
  • Purpose: A static loading test was performed to evaluate the ultimate flexural strength of a girder in which 80MPa high-strength concrete was synthesized on the compressive flange of the I-shape steel girder. Method: This test is designed and fabricated two types of specimens with different shear-connection specifications, and evaluated their ultimate flexural behavior until reaching the extreme event limit states. In addition, the ultimate strength was evaluated by comparing the test results and the results of the strain compatibility method. Result: By confirming the displacement within 0.02mm as a result of the relative slip measurement, it was verified that the two specimens secured perfect bonding. Therefore, the difference in the shear specification does not have a great effect on the stiffness, and if the specimens are completely synthesized, there is no difference in the behavior until it reaches the extreme-event limit states. Conclusion: The girder to be tested has a working load within the elastic range and meets the usability requirements for allowable deflection. Therefore, even if a part of the casing is subjected to the tensile force at the level of cracking, the deck will first reach the compression failure due to the role of the reinforcing bar.

Analysis of Propagation Environment for Selecting R-Mode Reference and Integrity Station (R-Mode 보정국과 감시국 선정을 위한 전파환경 분석에 관한 연구)

  • Jeon, Joong-Sung;Jeong, Hae-Sang;Gug, Seung-Gi
    • Journal of Navigation and Port Research
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    • v.45 no.1
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    • pp.26-32
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    • 2021
  • In ocean field, the spread of the Fourth Industrial Revolution based on information and communication technology requires high precision and stable PNT&D (Position, Navigation, Timing and Data). As the IMO (International Maritime Organization) and IALA (The International Association of Marine Aids to Navigation and Lighthouse Authorities) are requiring backup systems due to mitigate vulnerabilities and the increase of dependency on GNSS (Global Navigation Satellite System), Korea is conducting a research & development of R-Mode. An DGPS (Differentiate Global Positioning System) reference station that uses MF, an existing maritime infrastructure, and AIS (Automatic Identification System) base stations that use 34 integrity station and VHF will be utilized in this study to avoid redundant investment. Because there are radio shadow areas that display low signal levels in the west sea, the establishment of new R-Mode reference and integrity station will be intended to resolve problems regrading the radio shadow area. Because the frequency has a characteristic in that radio wave transmits well along the ground (water surface) in low frequency band, simulation and measurement were conducted therefore this paper to propose candidate sites for R-Mode reference and integrity station resulted through p wave's propagation characteristics analysis. Using this paper, R-Mode reference and integrity station can be established at appropriate locations to resolve radio shadow areas in other regions.