• Journal of the Optical Society of Korea
• /
• v.19 no.3
• /
• pp.228-236
• /
• 2015

Fluorescence recovery after photobleaching (FRAP) is a well-established method commonly used to measure the diffusion of fluorescent solutes and biomolecules in living cells or tissues. Here a fiber-optic-based FRAP (f-FRAP) system was developed, and validated using macromolecules in water and agarose gels of different concentrations. We applied f-FRAP to measure the site-specific diffusion of fluorescein (NaFluo) in peritoneal membranes (PMs) on the liver, cecum, and kidney of a living rat during peritoneal dialysis. Diffusion of fluorescein in PM varied in a time-dependent manner according to the type of organ ($D_{PM\;on\;Liver}/D_{NaFluo}=0.199{\pm}0.085$, $D_{PM\;on\;Cecum}/D_{NaFluo}=0.292{\pm}0.151$, $D_{PM\;on\;Kidney}/D_{NaFluo}=0.218{\pm}0.110$). The proposed method allows direct quantitative measurement of the three-dimensional diffusion in local PM in vivo, which was previously inaccessible by peritoneal function test methods such as peritoneal equilibration test (PET) and standardized PM assessment (SPA). f-FRAP is promising for local and dynamic assessments of peritoneal pathophysiology and the mass transport properties of PMs, presumed to be affected by variation of tissue structures over different organs and functional changes of the PM with years of peritoneal dialysis.

• Journal of Korean Society of Forest Science
• /
• v.86 no.2
• /
• pp.167-176
• /
• 1997

Characteristics of leaf morphology and vegetation were examined in 6 populations of rare endemic tree species, Koelreuteria paniculata Laxm. Additionally we studied genetic variation employing isozyme marker. Nested anova showed that there were statistically significant differences among populations as well as among individuals within populations in all 8 leaf morphological traits. In 7 of 8 traits, variance components among populations were larger than those among individuals within populations. Only a few tree species were found within each population and all the populations seemed to have been disturbed by man. Level of genetic diversity for this rare endemic species was very low, especially compared with those of geographically widespread tree species(A/L=1.1, P=9.5%, $H_o=0.021$, $H_e=0.035$) and degree of genetic differentiation was high($F_{ST}=0.114$). Factors contributing to the low level of genetic diversity and high level of genetic differentiation might include genetic drift, inbreeding and limited gene flow. Finally, implications for the conservation of genetic diversity of Koelreuteria paniculata were discussed.

• Journal of Korean Society of Forest Science
• /
• v.107 no.2
• /
• pp.151-157
• /
• 2018

This study was to analyze the genetic diversity of Quercus gilva Blume growing in Jeju Island for developing a preservation strategy. We examined the genetic diversity and structure using 6 ISSR primers and investigated 67 polymorphic ISSR amplicons in 80 trees distributed among five populations. The average of proportion of polymorphic loci were 93%, the average level of Shannon's information index was 0.237, and Nei's genetic diversity was 0.156. According to the analysis of the molecular variance (AMOVA), $F_{st}$ was 0.169 indicating there was a genetic variation among five populations. 17% of the total variation was allocated among the five populations, while the other 83% of the total variation was in individual trees in each population. The result could be due to the uneven number of trees among the five populations. Based on these results, the preservation strategy could be developed, for examples, considering for designation as "forest genetic resources conservation area" about the habitat, monitoring continuously, fostering the growth of seedling, ex situ preservation of genetic resources, and comparing the differences of environmental and genetic characteristic with population in ex situ.

• Korean Journal of Plant Resources
• /
• v.28 no.2
• /
• pp.279-289
• /
• 2015

Genetic diversity and genetic differentiation of thirteen Pinus densiflora populations in South Korea were estimated using nine ESTP (Expressed Sequence Tag Polymorphism) markers. The numbers of allele and the effective allele were 2.2 and 1.8, respectively. The percentage of polymorphic loci (P) was 98.8%. The observed and the expected heterozygosity were 0.391 and 0.402, respectively, and the eleven populations except for Ahngang and Gangneung population were under Hardy-Weinberg equilibrium state. The level of genetic differentiation (Wright’s F_ST = 0.057) was higher than those of isozyme or nSSR markers. We could not find out any relationship between the genetic distance and geographic distribution among populations from cluster analysis. Also, the genetic differentiation between populations was not correlated with the geographic distance (r = 0.017 and P = 0.344 from Mantel test). From the result of F_ST-outlier analysis to identify a locus under selection, six loci were detected at confidence interval of 99% by the frequentist’s method. However, only three loci (sams2+AluⅠ, sams2+RsaⅠ, PtNCS_p14A9+HaeⅢ) were presumed as outliers by Bayesian method. The sams2+AluⅠ and sams2+RsaⅠlocus were originated from the sams2 gene and seemed to be the loci under balancing selection.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.12
• /
• pp.1680-1685
• /
• 2002

Reproductive data collected from more than 20,000 Japanese Black cows of Hyogo and Shimane Prefectures were analyzed. Averages of age at first calving, gestation length, days open and calving interval were 25.1 mo, 289 d, 112 d and 401 d, respectively. Variance components were obtained by REML procedure and the heritability estimate of age at first calving was 0.22. In gestation length the heritability estimate was 0.40 and no permanent environmental effect was estimated. Estimated variance components of calving interval were similar to those of days open and the heritability and repeatability of calving interval were 0.05 and 0.09, respectively. Random farm effects accounted for approximately 10% of phenotypic variations in all traits. Genetic and farm correlations between age at first calving and calving interval were 0.27 and 0.39, respectively. It was found that temporary environment was an important source of variation for calving intervals of Japanese Black.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.11
• /
• pp.1621-1627
• /
• 2001

Steers and heifers (N=490) were produced between 1991 and 1996 by reciprocal fiillsib backcross and $F_1$ crosses from Angus and Brahman to compare characteristics of carcass and palatability traits between Bos indicus and Bos taurus inheritance. Carcasses of 3/4Angus were heavier, fatter (p<0.05), more tender and higher in other palatability attributes (p<0.01) than those of 3/4Brahman. Reciprocal effects of parental cross breeds were found on some traits. Within 3/4Brahman inheritance group, Brahman sired progeny produced heavier and fatter carcasses with better palatability (p<0.05) than progeny with Brahman as a dam breed. Estimates of heritability were intermediate to high in most carcass and palatability traits. Genetic correlations of tenderness with marbling score (MARB), sarcomere length (SARC), fragmentation index (FRAG) and calpastatin activity (CALP) were moderate to high, suggesting potential use of the tenderness-influencing factors as indirect selection criteria to improve palatability attributes. MARB and SARC that were best predictors of tenderness explained 3.07 to 5.85% and 4.32 to 8.24% of variation in tenderness, respectively. However, there was no tenderness-influencing factor to dominantly explain large portion of variation in tenderness.

• Journal of Preventive Medicine and Public Health
• /
• v.27 no.3 s.47
• /
• pp.609-626
• /
• 1994

This study was conducted to investigate whether variations in hospital services utilization across small geographic areas in Korea existed, and if so, what factors are responsible for the variation. The claims data of the fiscal year 1992 obtained from the regional health insurance societies were used for the study. Main findings of the research can be summarized as follows: 1 Extremal Quotients (EQ) of hospital expenditure per capita and hospital days per capita were 2.69 and 2.73, and Coefficient of Variation (CV) were 0.14, both, respectively. The EQ and the CV of admission rate were also 2.71, 0.15. The EQ and the CV of expenditure per admission were 1.73, 0.10 and those of hospital days per admission were 1.29, 0.06. All these statistics were statistically significant and this result provides strong evidence for the existence of small area variations. 2. Comparing patterns of variation among areas, the area which showed higher utilization amounts is Chansungp'o. Koje area, whereas the areas which showed lower utilization amounts are Yongju, Changhung, Miryang, Mokp'o, Koch'ang area. 3. Multivariate analytic methods were used to examine factors related to the variation across areas. In terms of the health resource availability variables, beds per capita or physicians per capita were positively associated with all utilization indices. As for the health service market structure variables, the proportion of health care institutions operating for less than f years was positively related to the expenditure per capita, hospital days per capita and expenditure per admission. In addition the proportion of the private health care institutions also had a negative relationship with total utilization amount and admission rate and the proportion of physicians under age 40 was negatively associated with expenditure per capita and expenditure per admission. With regard to the socio-demographic characteristics, proportion of medicaid population was positively related to hospital days per capita, and percentage of paved road was positively related to hospital days per admission. As a conclusion, wide variations existed across small areas in Korea and supply factors were found to be important in explaining the variation.

• Current Research on Agriculture and Life Sciences
• /
• v.20
• /
• pp.1-8
• /
• 2002

This study was conducted to obtain the information of varietal development using wild soybean through investigation of variation and heredity of major agronomic characters in $F_2$ generation of interspecific cross between Glycine max and G. soja. In segregating populations of two crosses, all characters observed except 100-seed weight showed transgressive segregation. Days to flowering showed normal distribution; mean days to flowering in $F_1$, and $F_2$ was approximately mean of parent. Mean of $F_1$ for 100-seed weight was 6.2g and 5.7g for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which were somewhat skewed distribution to small seeded parents. Mean seed yield of $F_2$ was about mean of two parents. Degree of vine was 6.4 and 5.7 in $F_1$ for two crosses but it was 6.0 in $F_2$ for Eonbakong/KLG10084, which revealed the degree of vine as partial dominance while 4.6 for Sobaegnamulkong/KLG10084, somewhat different results from the previous cross. Broad-sense heritability($h^2B$) for plant height, days to flowering, pods per plant, seed yield, and degree of vine was comparatively high and narrow-sense heritability($h^2B$) for 100-seed weight which is the moot important character in the development of small seed-size sprout soybean was 52.3% and 65.6% for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which indicated that selection for the character in early generation was possible.

• Journal of Oral Medicine and Pain
• /
• v.30 no.4
• /
• pp.427-432
• /
• 2005

This study have been compared and analyzed voice parameter under the condition of normal voice and auditory condition(noise and music) for 29 patients of orofacial pain and 31 normal people to investigate voice feature and vocal variation for auditory condition of orofacial pain patient. 1. Compared to normal voice, orofacial pain patient showed lower and unstable voice feature which has low F0 rate and high jitter and shimmer rate. 2. Voice of orofacial pain patient showed more relaxed and stable voice feature with low F0 and shimmer rate in the music condition than noise condition. 3. Normal people's voice has no significant difference between music and noise condition even though it has high F0 rate under the noise condition. As a result, orofacial pain patient showed difference of feature and different response for external auditory condition compared to normal voice. Providing of positive emotional environment such as music could be considered for better outcome of oral facial pain patient's functional disability.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.11
• /
• pp.1693-1699
• /
• 2015

Overall quality of six kinds of commercial broiler breast fillets (A~F) retailed in Korea were examined, including proximate composition analysis, shape profile analysis, pH, color, tenderness, cooking loss, descriptive analysis, and consumer testing. A total of 120 breast meat fillets were purchased at a local market. Overall quality of broiler breast meat commercially available in Korea was found to vary significantly, indicating a broad range of product quality in the marketplace. Moisture content of meat ranged from 74.4 to 76.4%, whereas protein, fat, and ash contents were 21.8~25.2, 0.2~0.8, and 1.07~1.16%, respectively. The pH ranged from 6.2 to 6.4, whereas color values ($L^*$, $a^*$, and $b^*$) were 52.2~57.2, -3.5~0.5, and 8.3~13.6, respectively, among all products tested. Approximately 9% variation in cooking loss was detected depending on the products evaluated, whereas shapes (height, width, and length) of meat were also significantly different (15% variation on average). Product F was proven to be the least tender and least liked by consumers, implying that tenderness is a key attribute determining consumer acceptability of broiler breast meat.