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Supplementation of Indigenous Green Microalga (Parachlorella sp.) to Pre-starter Diet for Broiler Chickens (초기 육계 사료내 토착미세조류(Parachlorella sp.) 첨가에 따른 성장 및 면역반응 변화)

  • An, Su Hyun;Joo, Sang Seok;Lee, Hyo Gun;Kim, Z-Hun;Lee, Chang Soo;Kim, Myunghoo;Kong, Changsu
    • Korean Journal of Poultry Science
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    • v.47 no.1
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    • pp.49-59
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    • 2020
  • The present study determined the effect of dietary cultivated microalgae (Parachlorella sp.) on the growth and immune responses of pre-starter broilers. A total of 320 one-day-old birds (Ross 308) were allocated to 4 treatments with 8 blocks in a randomized complete block design. The four experimental diets consisted of a corn-soybean meal-based control diet, and three diets contained 0.5%, 1.0%, and 1.5% microalgae powder at the expense of cornstarch in the control diet. After feeding the experimental diets for 7 days, the body weight and feed intake of all birds were measured, and 8 birds were randomly selected from each treatment. Peripheral blood mononuclear cells (PBMCs) and serum were harvested for immune profile assessment, including cytokines and cell migration receptors. No differences in growth performance were observed among the treatments. The birds that were fed diets containing graded levels of microalga showed a linear increase in the mRNA expression of cytokine genes in PBMCs, including that of IL2, IL1β, and IL18 (P<0.05). With respect to the chemokine receptor genes in PBMCs, mRNA expression of CCR2, CCR9, and ITGA4 changed quadratically (P<0.05), but that of CCR7 increased linearly (P<0.01). Cytokine protein secretion in blood, including that of IL-1β and IL-6, increased linearly (P<0.01) with an increase in the microalgal content. Overall, the present results show that the indigenous microalgae powder used in this study could stimulate immunity with no detrimental effects on the growth performance of pre-starter broiler chickens.

Imaging Performance Analysis of an EO/IR Dual Band Airborne Camera

  • Lee, Jun-Ho;Jung, Yong-Suk;Ryoo, Seung-Yeol;Kim, Young-Ju;Park, Byong-Ug;Kim, Hyun-Jung;Youn, Sung-Kie;Park, Kwang-Woo;Lee, Haeng-Bok
    • Journal of the Optical Society of Korea
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    • v.15 no.2
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    • pp.174-181
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    • 2011
  • An airborne sensor is developed for remote sensing on an aerial vehicle (UV). The sensor is an optical payload for an eletro-optical/infrared (EO/IR) dual band camera that combines visible and IR imaging capabilities in a compact and lightweight package. It adopts a Ritchey-Chr$\'{e}$tien telescope for the common front end optics with several relay optics that divide and deliver EO and IR bands to a charge-coupled-device (CCD) and an IR detector, respectively. The EO/IR camera for dual bands is mounted on a two-axis gimbal that provides stabilized imaging and precision pointing in both the along and cross-track directions. We first investigate the mechanical deformations, displacements and stress of the EO/IR camera through finite element analysis (FEA) for five cases: three gravitational effects and two thermal conditions. For investigating gravitational effects, one gravitational acceleration (1 g) is given along each of the +x, +y and +z directions. The two thermal conditions are the overall temperature change to $30^{\circ}C$ from $20^{\circ}C$ and the temperature gradient across the primary mirror pupil from $-5^{\circ}C$ to $+5^{\circ}C$. Optical performance, represented by the modulation transfer function (MTF), is then predicted by integrating the FEA results into optics design/analysis software. This analysis shows the IR channel can sustain imaging performance as good as designed, i.e., MTF 38% at 13 line-pairs-per-mm (lpm), with refocus capability. Similarly, the EO channel can keep the designed performance (MTF 73% at 27.3 lpm) except in the case of the overall temperature change, in which the EO channel experiences slight performance degradation (MTF 16% drop) for $20^{\circ}C$ overall temperate change.

Determination of ethyl carbamate in maesil wine by alcohol content and ratio of maesil (Prunus mume) during ripening period (알코올 농도 및 담금비에 따른 숙성 기간별 매실주의 에틸카바메이트 함량조사)

  • Kim, Nan-Young;Eom, Mi-Na;Do, Young-Sook;Kim, Jung-Beom;Kang, Suk-Ho;Yoon, Mi-Hye;Lee, Jong-Bok
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.429-434
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    • 2013
  • This study was designed to investigate the formation of ethyl carbamate (EC) during the ripening of Maesil with sugar and Soju (19.5~35% alcohol contents) using a homemade method. Maesil, sugar and Soju were purchased at ordinary market in June of 2012. The preparation of sample for analysis was conducted by method of Henry et al. The analysis of GC/MS was used SIM mode (m/z 89, 74, 62). Quantification was performed in terms of the 62 ion and was based on an internal standard procedure. Good linearity was obtained with a regression coefficient ($r^2$ = 0.993). Low detection limits (LOD) was achieved 4.31 ug/kg and recovery for alcohol was 74.8%. During 90 days, fermentation with sugar was not detected EC (under LOQ). 15 days ripened Maesil wine contained EC between non detected~32.7 ug/kg and 90 days ripened Maesil wine was 19.7~87.4 ug/kg. Higher proportion of Maesil and Soju increased EC contents. EC levels were increased 32.7 ug/kg to 87.4 ug/kg in the ratio of Maesil to 35% alcohol-Soju (1:1). In the ratio of Maesil to 35% alcohol-Soju (1:3) was increased non detected to 69.7 ug/kg. After 90 days, Maesil wine was filtered Maesil through a seive and ripened by 180 days to investigate the formation of EC compared with non filtered. Treatment of filtered, EC contents was much higher level compared with non filtered. Therefore, this result showed that alcohol contents contribute to increase EC formation more than Maesil.

Effects of the Acute Exposure Oxytetracycline on the Behavior and Endocrine Response in Adult Zebrafish (Oxytetracycline의 단기 노출이 제브라피쉬의 행동 및 내분비 반응에 미치는 영향)

  • Ko, Eun Seong;Lee, Seungheon
    • Journal of Life Science
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    • v.25 no.2
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    • pp.151-157
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    • 2015
  • Zebrafish (Danio rerio) has been more widely used to study pharmacology. Oxytectracycline (OTC) is a broad-spectrum antibiotic and works by interfering with the ability to produce essential proteins of bacteria. The aim of this study was to identify the effects of exposure to OTC on behavioral changes or endocrine response in zebrafish. The behavioral effects of exposure to OTC (50, 100 or 200 mg/l) were characterized in several novelty-based paradigms such as the novel tank or open field test in zebrafish. Moreover, to investigate effects of exposure to OTC on endocrine response, we measured whole-body cortisol level using cortisol ELISA kit. As results of novel tank test, duration in top and immobile duration were significantly increased by the exposure to OTC in a concentration-dependent manner (p<0.05). In addition, moving distance, highly mobile, velocity and zone transition were significantly decreased by the exposure to OTC in a concentration-dependent manner (p<0.05). As results of open field test, the exposure to OTC increased immobile duration significantly (p<0.05). However, moving distance, mobile duration and velocity were significantly decreased by the exposure to OTC in a concentration-dependent manner (p<0.05). Besides, the exposure to OTC elevated whole-body cortisol levels in zebrafish. These results suggest that the exposure to OTC may induce chemical stress in zebrafish.

Effects of AEBSF on the Delay of Spontaneous Apoptosis and the Trans-Differentiation of Human Neutrophils into Dendritic Cells (Serine pretease 억제제인 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF)에 의한 호중구의 자연 세포사멸의 지연과 수지상 세포로의 전이분화 연구)

  • Park, Hae-Young;Kwak, Jong-Young
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.948-955
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    • 2007
  • Neutrophils play a key role as a first line of defense and are known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. The spontaneous apoptosis of neutrophils was delayed by treatment with 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF), a serine protease inhibitor. AEBSF inhibited both caspase-3 and serine protease activities, whereas ZVAD-fmk, a pancaspase inhibitor, inhibited only caspase-3 activity. The life span of neutrophils was prolonged up to 5 days by AEBSF in the presence or absence of granulocyte macrophage colony stimulating factor(CM-CSF). DC surface markers, such as CD80, CD83, and MHC class ll were not expressed on neutrophils treated with AEBSF alone. CM-CSF failed to prolong the survival time of neutrophils up to3 days but increased the expression levels of DC markers on neutrophils in the presence of AEBSF. Expression levels of DC markers were the highest on neutrophils treated with CM-CSF and AEBSF for 3 days. AEBSF and CM-CSF-treated neutrophils stimulated proliferation of T cells in the presence of a superantigen, Staphylococcal enterotoxin B (SEB) but produced $interferon-{\gamma}$ ($IFN{\gamma}$) in the absence of SEB. These results suggest that the inhibition of serine protease activity prolonged the life span of human neutrophils and combined treatment of neukophils with CM-CSF and serine protease inhibitor induced differentiation of neutrophils into DC-like cells.

Characteristics of Flower of Plus Tree Clones of Pinus koraiensis S. et Z. (잣나무 수형목(秀型木) clone의 개화특성(開花特性))

  • Han, Sang Sup;Lee, Sang Boong
    • Journal of Korean Society of Forest Science
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    • v.79 no.3
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    • pp.290-301
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    • 1990
  • The 167 plus tree clones of Pinus koraiensis in clone bank planted in 1983 were investigated for time of flowering, rate of flowering, and number of flowering from 1986 to 1989. The results were as follows : 1) There were clones in the minority which do not cross in natural pollination between earlest flowering female clones and latest shedding male clones. 2) The rate of male flowering appeared less than rate of female flowering and received influence of genetic more than rate of female flowering. 3) The numbers of flowering in female and male flower were affected by small number of clones. 4) The flowering number for female strobili was not related to the flowering number of male flower. The 42 clones among 167 clone had not male flower. 5) The flowering number of 167clones were classified three groups in female flower and four groups in male flower by L.S.D 5% test. 6) The clones with abundant female and male flower were selected based on the component analysis. 7) As Based on flowering rate and number of female, the juvenile phase of plus tree clones appeared to be until four years after grafting ; the transition period appeared to be from five to seven years after grafting ; the adult phase appeared to begin from eight years after grafting, 8) The grafted trees of Pinus koraiensis appeared early flowerring about six years compared with seedling trees.

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Study on the Fish Community in the Seagrass Belt around Cheju Island II. Growth, reproduction and food habit of Tubesnout, Aulichthys japonicus Brevoort (제주도 연안 해초지대의 어류군집에 관한 연구 II. 실비늘치 (Aulichthys japonicus Brevoort)의 성장, 산란 및 식성)

  • Go, You-Bong;Cho, Sung-Hwan;Go, Gyung-Min
    • Korean Journal of Ichthyology
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    • v.9 no.1
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    • pp.61-70
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    • 1997
  • The spawning season, growth and feeding habit of tubesnout, Aulichthys japonicus were examined from the samples collected by a small beam trawl in the Zostera marina belt at the coastal water off Hamduck, Cheju Island, from May 1993 to May 1994. Tubesnout was the dominant species of overall 58 species collected in the study area throughout the year. The larvae and juvenile of tubesnout in the frequency distributions of body length began to appear in the Z. marina belt in March, and adults disappeared after May in the following year. The gonad weight index (GWI) and the condition factor (K) were high from November 1993 to January 1994 for males, while females were high from February to May 1994. Although tubesnout is an oviparous fish, males have the urogenital papilla. These results suggest that they were fertilized between November and January, and then males seemed to be died or move into other places. Females were examined to spawn from February to May. The stomach contents of tubesnout were dominated by copepoda smaller than ca. 1mm, Paracalanus, Oithona, Acartia, Oncaea and Harpacticoidae, accounting for 97% of total food items. The feeding habit of tubesnout did not vary by size. The fishes fed on Calanus, Euchaeta and decapoda larvae larger than ca. 3mm in March and April during the spawning season.

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GIS-Based Suitability Assessment Plan of Coastal Zoning System (GIS 기반 연안 용도해역 적성평가 방안)

  • Lee, Geun-Sang;Lim, Seung-Hyeon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.16 no.2
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    • pp.75-87
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    • 2013
  • This study developed a GIS-based suitability assessment model of coastal zoning system that is needed in the substantial classification of coastal zoning system according to the establishment of law about coastal zoning system. First, this study investigated several kinds of regulations, GIS database and application system related coastal area. Also, grid data model was selected as the GIS analytical model for calculating items of suitability assessment of coastal zoning system. And Grid-based analytical method was suggested for calculating items composing of sea and spatial location characteristics including physical one. Critical values of items were presented using standards that were suggested in coastal regulations and land suitability assessment. Especially, this study presented a calculation method of continuous pattern as fuzzy set function for reflecting the characteristics of GIS data. And this study classified the suitability grade using Z-score and developed model designating coastal zone as conservation management priority, utilization management priority, and planning management priority. This study is judged that very efficient business performance is possible if we consider the spatial coverage of study area and GIS database when the suitability assessment model of coastal zoning system that is suggested in this study, is applied to business works.

Extragalactic Sciences from SPICA/FPC-S

  • Jeong, Woong-Seob;Matsumoto, Toshio;Im, Myungshin;Lee, Hyung Mok;Lee, Jeong-Eun;Tsumura, Kohji;Tanaka, Masayuki;Shimonishi, Takashi;Lee, Dae-Hee;Pyo, Jeonghyun;Park, Sung-Joon;Moon, Bongkon;Park, Kwijong;Park, Youngsik;Han, Wonyong;Nam, Ukwon
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.1
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    • pp.36.2-36.2
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    • 2013
  • The SPICA (SPace Infrared Telescope for Cosmology & Astrophysics) project is a next-generation infrared space telescope optimized for mid- and far-infrared observation with a cryogenically cooled 3m-class telescope. The focal plane instruments onboard SPICA will enable us to resolve many astronomical key issues from the formation and evolution of galaxies to the planetary formation. The FPC-S (Focal Plane Camera - Sciecne) is a near-infrared instrument proposed by Korea as an international collaboration. Owing to the capability of both low-resolution imaging spectroscopy and wide-band imaging with a field of view of $5^{\prime}{\times}5^{\prime}$, it has large throughput as well as high sensitivity for diffuse light compared with JWST. In order to strengthen advantages of the FPC-S, we propose the studies of probing population III stars by the measurement of cosmic near-infrared background radiation and the star formation history at high redshift by the discoveries of active star-forming galaxies. In addition to the major scientific targets, to survey large area opens a new parameter space to investigate the deep Universe. The good survey capability in the parallel imaging mode allows us to study the rare, bright objects such as quasars, bright star-forming galaxies in the early Universe as a way to understand the formation of the first objects in the Universe, and ultra-cool brown dwarfs. Observations in the warm mission will give us a unique chance to detect high-z supernovae, ices in young stellar objects (YSOs) even with low mass, the $3.3{\mu}$ feature of shocked circumstance in supernova remnants. Here, we report the current status of SPICA/FPC project and its extragalactic sciences.

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A STUDY ON PHYSICAL PROPERTIES OF FLOWABLE COMPOSITE RESINS (유동성 복합레진의 물리적 성질에 대한 연구)

  • Kim, Ji-Young;Jeong, Byung-Cho;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.3
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    • pp.423-429
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    • 2002
  • Recently, the clinical uses of flowable composite resins have increased because of fast, convenient and excellent accessibility, but little has been reported about physical properties of flowable resins. The purpose of this study was to measure and compare the physical properties(compressive strength, relative flowability, relative radiopacity) of 4 contemporary flowable composite resins(Filtek flow, Tetric Flow, Revolution Palfique Estelite LV high flow). The results were as follows; 1. There were no significant differences between 4 flowable composite resins in compressive strength, but all were lower than that of traditional hybrid composite resin(p<0.001). 2. The relative flowability were increased in order of Palfique Estelite LV high flow, Revolution, Filtek flow, Tetric Flow and sealant(p<0.001), but there were no significant differences between Filtek flow and Tetric Flow. 3. There were significant differences between flowable composite resins in relative radiopacity and they showed similar or higher radiopacity than dentin(p<0.001), especially Tetric Flow and Filtek Flow showed higher radiopacity than enamel(p<0.001). This results suggested that the stress of application area have to be considered since flowable composite resins have lower compressive strength than hybrid composite, and the differences of flowability between these flowable composite resins can be considered when they are selected. All tested flowable composite resins showed optimal radiopacity to ISO's recommend.

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