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Follow-up Comparison of Two Different Types of Anterior Thoracolumbar Instrumentations in Trauma Cases : Z-plate vs. Kaneda Device

  • Park, Jung-Keun;Kim, Keun-Su
    • Journal of Korean Neurosurgical Society
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    • v.41 no.2
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    • pp.77-81
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    • 2007
  • Objective : In a variety of thoracolumbar diseases, corpectomy followed by interbody bone graft and anterior instrumentation has allowed direct neural decompression and reconstruction of the weight-bearing column by short segments fusion. In this study, we compared spinal stability of the two different anterior thoracolumbar instruments : Z-plate and Kaneda device representing plate and two-rods type, respectively. Methods : A retrospective review was performed for all the patients with thoracolumbar diseases or traumas treated with anterior corpectomy, autologous iliac bone graft, and fixation with instruments from 1996 to 2000. For the anterior instrumentation, Z-plate or Kaneda device was used for 24 [M:F=5:9, average age=37] and 12 [M:F=9:3, average age=41] patients, respectively. The plain AP and lateral flexion-extension films were taken immediately after surgery and at each follow-up. The sagittal and coronal Cobb's angles at the operation segments were used to observe the change of initial fixation status. The surgical time length and bleeding amount of the two groups were compared. Intra-operative and post-operative instrument associated complications were evaluated. Student t-test was used for statistical analysis and p-value less than 0.05 was considered to be significant. Results : Mean follow-up durations for Z-plate and Kaneda device were 24 and 21 months, respectively. The fusion rate was 91% for Z-plate and 100% for Kaneda device. Two cases of Z-plate group showed instrumentation failure during the follow up period, in which additional surgery was necessary. The mean differences of sagittal Cobb's angles among the AP images immediate after surgery and at follow-up were 7 and 2 degrees for Z-plate and Kaneda device, respectively [p<0.05]. The mean differences of coronal Cobb's angles were 5 and 2 degrees for Z-plate and Kaneda device, respectively [p<0.05]. No Intra-operative complication has occurred in both groups. There was no difference in surgery time and bleeding amount between two groups. Conclusion : We think that Kaneda device [rod type] is stronger than Z-plate [plate type] to keep the spinal stability after anterior thoracolumbar surgery.

Construction of a Baculovirus Expression System Using Hyphantria cunea Nuclear Polyhedrosis Virus for Eukaryotic Cells

  • Lee, Hyung-Hoan;Kang, Bong-Joo;Park, Kap-Ju;Cha, Soung-Chul
    • Journal of Microbiology and Biotechnology
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    • v.8 no.6
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    • pp.676-684
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    • 1998
  • Baculovirus transfer and expression vectors with Hyphantria cunea nuclear polyhedrosis virus (HcNPV) were constructed. An initial transfer vector, pHcEV, constructed using HcNPV was previously reported (Park et al. 1993. J. Kor. Soc. Viral. 23: 141-151). Herein, the size of the vector was properly reduced, and a functionally perfect vector was constructed and named pHcEV-IV (6.7 kb). The vector has a 2.2-kb HcNPV DNA sequence in the 5'-flanking region of the vector's polyhedrin gene promoter. The 1.8-kb HcNPV DNA sequence, poly A signal sequence, T3 primer sequence, and 13 multicloning site sequences, in order, were ligated in front of the translation start codon of the polyhedrin gene. The cloning indicating marker lacZ gene was inserted into the pHcEV-IV, named pHcEV-IV-lacZ, and transferred into the wild-type virus. Recombinant expression virus, lacZ-HcNPV, was constructed by replacing the lacZ gene in the pHcEV-IV-lacZ with the polyhedrin gene of the wild-type virus. The recombinant virus was isolated from blue plaques that produce $\beta$-galactosidase without polyhedra. The lacZ gene insertion was confirmed by Southern hybridization analysis. The expression of the lacZ gene in Spodoptera frugiperda cells infected with the lacZ-HcNPV was examined by SDS-PAGE and colorimetric assay. One 116-kDa LacZ protein band appeared on the PAGE. The production rate of the $\beta$-galactosidase was approximately 50 international units (IU) per min per ml between 2 to 5 days postinfection (p.i.). The highest activity occurred at five days p.i. was 170 IU/min/$m\ell$. The enzyme activity first appeared about 20 h p.i. as measured by colorimetric assay.

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Reproductive Phenology of Four Korean Seagrasses, Zostera caespitosa, Z. caulescens, Z. japonica and Z. marina (한국산 해초 포기거머리말, 수거머리말, 애기거머리말과 거머리말의 생물계절학)

  • Lee, Sung-Mi;Lee, Sang-Yong;Choi, Chung-Il
    • Ocean and Polar Research
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    • v.27 no.2
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    • pp.125-133
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    • 2005
  • This study described the phonology and reproductive potential of four species of Korean seagrasses, Zostera caespitosa, Z. caulescem, Z. Japonica and Z. marina. Z. caespitosa and Z. caulescens sampled from a mixed stand at the subtidal area of Yulpo Bay, Geojedo of the South Sea of Korea in November 2002 and August 2003. Z japonica and Z. marina occurred at the depth between the middle intertidal and shallow subtidal (<1m below mean sea level) of Seungbongdo (in Yellow Sea) samples collected in February and October 2003. The sexual reproductive phase of the four Zostera species was apparently different in timing of flowering, reproductive period, fruiting and seed maturing. Z. caespitosa flowered from February to early May $(10-16^{\circ}C)$, and its seed production completed in early May. The reproductive shoots of Z. caulescens began to appear in January $(9^{\circ}C)$, and its flowering followed from February to June $(10-19^{\circ}C)$. The flowers of Z. japonica were observed from July to September $(18-22^{\circ}C)$, and its seeds matured from August to September. The most commonly I marina flowered from April to August $(7-21^{\circ}C)$ and developed into seeds in July. Z. caulescens, the largest plant, had the highest number of seeds per shoot and longest spadix length. Z. marina, which was intermediate In size, recorded the highest reproductive potential. The study indicates that the reproductive phase and potential of the four species of seagrass from Korea are highly related to water temperature, and the populations of these species show a perennial lifespan with a low sexual reproductive input.

Mathematical Modeling of Self-propagating High Temperature Synthesis of Molybdenum- Tungstenb Disilicide (이규화 몰리브덴-텅스텐의 자전 고온 합성 반응 모델링)

  • Yeon, Sun-Hwa;Jang, Dae-Gyu;Lee, Cheol-Gyeong
    • Korean Journal of Materials Research
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    • v.11 no.3
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    • pp.164-170
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    • 2001
  • The Self-propagating High-temperature Synthesis (SHS) for synthesizing ($Mo_{1-z}$ , $W_{z}$)$Si_2$was conducted experimentally with the mole fraction of Tungsten(W) from z=0.0 to z=0.5. The temperature profile was measured according to the reaction time through the thermocouple that was equipped into the center of these samples. When the reaction front is propagated around the thermocouple, the highest temperature appears and we regard this temperature as the adiabatic temperature. We found out by experimental results that the reaction velocity is in the range of 2.14~1.35mm/sec and the adiabatic temperature is in the range of 1883~1507K for the six samples. The reaction velocity and the adiabatic temperature were inclined to decrease with an increasing of the mole fraction of Tungsten (W). The SHS modeling is presented in order to predict the temperature profiles and these results are compared with the experimental results. It is predicted that in case of increasing the initial temperature of these six samples, the reaction temperature increased and that the sample of z=0.5 needs the preheating up to 800~900K in order to become reaction temperature 1900K.

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Analysis of Amyloid Beta 1-16 (Aβ16) Monomer and Dimer Using Electrospray Ionization Mass Spectrometry with Collision-Induced Dissociation

  • Kim, Kyoung Min;Kim, Ho-Tae
    • Mass Spectrometry Letters
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    • v.13 no.4
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    • pp.177-183
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    • 2022
  • The monomer and dimer structures of the amyloid fragment Aβ(1-16) sequence formed in H2O were investigated using electrospray ionization mass spectrometry (MS) and tandem MS (MS/MS). Aβ16 monomers and dimers were indicated by signals representing multiple proton adduct forms, [monomer+zH]n+ (=Mz+, z = charge state) and [dimer+zH]z+ (=Dz+), in the MS spectrum. Fragment ions of monomers and dimers were observed using collision-induced dissociation MS/MS. Peptide bond dissociation was mostly observed in the D1-D7 and V11-K16 regions of the MS/MS spectra for the monomer (or dimer), regardless of the monomer (or dimer) charge state. Both covalent and non-covalent bond dissociation processes were indicated by the MS/MS results for the dimers. During the non-covalent bond dissociation process, the D3+ dimer complex was separated into two components: the M1+ and M2+ subunits. During the covalent bond dissociation of the D3+ dimer complex, the b and y fragment ions attached to the monomer, (M+b10-15)z+ and (M+y9-15)z+, were thought to originate from the dissociation of the M2+ monomer component of the (M1++M2+) complex. Two different D3+ complex geometries exist; two distinguished interaction geometries resulting from interactions between the M1+ monomer and two different regions of M2+ (the N-terminus and C-terminus) are proposed. Intricate fragmentation patterns were observed in the MS/MS spectrum of the D5+ complex. The complicated nature of the MS/MS spectrum is attributable to the coexistence of two D5+ configurations, (M1++M4+) and (M2+M3+), in the Aβ16 solution.

Systematic Study on the Genus Zacco (Pisces, Cyprinidae). II. Phylogenetic Relationships of the Genera Zocco and Candidia (피라미속(잉어목, 잉어과) 어류의 계통분류학적 연구 II. Zacco속 및 Candidia속 어류의 계통적 유연관계)

  • 민미숙;양서영
    • The Korean Journal of Zoology
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    • v.34 no.4
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    • pp.571-584
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    • 1991
  • 잉어과(Gyprinidae)의 Zocco속 어류 4종과 Candidia속 어류 1종에 대한 종간 유연관계와 종분화 연대측정 및 이들의 지질학적 분포경로를 밝히기 위하여 한국, 일본 및 대만에서 채집된 개체를 대상으로 전기영동법에 의한 유전자 분석을 하였다. 각 종의 지역별(한국, 일본, 대만) 집단간 유전적 유연관계를 분석한 결과 평균 유전적 근연치는 90% 이상이었다. Z. temmincki의 경우 일본집 단들은 한국의 A-type 집단과는 유연관계가 가까왔으나 한국의 B-type과는 유전적 차이가 현저하러다. Z. PlaD여5의 경우 한국집단과 일본집단사이의 유연관계는 S = 0.852였고, 한국집단과 대만집단 사이는 5 = 0.672, 일본집단과 대만집단 사이는 S = 0.751로서 지리적으로 현저한 차이를 업였다. Z. pochycephalus 3개 집단간의 유전적 근인치는 S = 0.963이었고 Candidia borbota 2개 집단간은 S = 0.946이었다. 종간의 유전적 근연환계를 비교한 결과 Candidia borbota와 Z. temmincki사이는 S = 0.608, Z. pluDpus와 1. pachycephalus사이는 S = 0.612였으나, Z. temmincki와 Z. platypus사이는 S = 0.441, Z. temmincki 와 Z. pochycepholus 사이는 S = 0.350이었고, Z. plotpus와 Condfda barbata사이는 S = 0.328로서 이들 사이에는 현저한 유전적 차이가 있었다. 각 종간의 롱분화 연대를 추산한 결과 이들은 약 480만년 전인 Pliocene 초기에 공통 조상종에서 분화하여 Z. temmincki, Candidia borbato group과 Z. plotypus, Z. pochycepholus group으로 분리되었고 약 260만년 전인 Pliocene 후기에 Z. temminc소와 Candidia borbota로 분화되었다고 추산되며 약 80만년 전인 Pleistocene시기에 남 temmincki B-type에서 h-type이 분화되었다짙 여겨진다. 한편 또 다른 한 단opP은 약 230만년 전인 열iocene후기에 대만 지역의 Z. plotypas에서 Z. pochvcepholus가 분화된 후 현재에 이르렀다고 추정된다. Z. platypus는 약150만년 이전인 초기 Pleistocene시기에 대만지역에서 한국 및 일본집단으로 분리되었다고 보며 이들 한국집단과 일본집단은 약 50만년 전 Pleistocene의 Middle기에 고황하 수계를 거쳐 현재의 분포 상황에 이르렀다고 여겨진다. 한편 대. temmin체과의 B-type에서 저온 적응으로 분화되었다고 추측되는 A-type은 약 20만년 전인 Pleistocene의 Riss기에 역시 고황하 수계를 통하여 한국과 일본으로 분포하여 현재에 이르렀다고 사료된다.

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Reduction of Residual Vibration for 2 Axes Overhead Crane by Input Shaping (입력성형기법에 의한 2축 천정크레인의 잔류진동 감소)

  • 박운환;이재원;노상현
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.4
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    • pp.181-188
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    • 2000
  • Input shaping is a method fur reducing residual vibration. Vibration is eliminated by convolving an input shaper, which is a sequence of impulses, with the desired system command. It has been applied to robot with a flexible manipulator. But it can be applied to the reduction of residual vibration far overhead crane. In this paper, input shaping shows good performance for anti-sway of overhead crane. In the z-domain, we designed an input shaper and calculated the sensitivity of it. If sensitivity is calculated in the z-domain, the shapes of sensitivity curves are expected easily. Accordingly, it is easy to design an input shaper in the z-domain. We compared the response of a system with shaper to it without that. Also, we compared El shaper to ZV shaper in view of robustness.

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THE HAMILTONIAN SYSTEM WITH THE NONLINEAR PERTURBED POTENTIAL

  • Jung, Tacksun;Choi, Q-Heung
    • Korean Journal of Mathematics
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    • v.15 no.2
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    • pp.195-206
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    • 2007
  • We investigate the multiplicity of $2{\pi}$-periodic solutions of the nonlinear Hamiltonian system with perturbed polynomial and exponential potentials, $\dot{z}= JG^{\prime}(z)$, where $z:R{\rightarrow}R^{2n}$, $\dot{z}={\frac{dz}{dt}}$, $J=\(\array{0&-I\\I&0}\)$, I is the identity matrix on $R^n,G:R^{2n}{\rightarrow}R$, G(0, 0) = 0 and $G^{\prime}$ is the gradient of G. We look for the weak solutions $z=(p,q){\in}E$ of the nonlinear Hamiltonian system.

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Characteristics of Electrowetting of Self-assembled Monolayer and Z-Tetraol Film

  • Lin Li-Yu;Noh Dong-Sun;Kim Dae-Eun
    • International Journal of Precision Engineering and Manufacturing
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    • v.7 no.3
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    • pp.35-38
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    • 2006
  • A study of electrowetting using an Octadecyltrichlorosilane (OTS) self-assembled monolayer (SAM) and Z- Tetraol 2000 perfluoropolyether lubricant as hydrophobic layers on Si and $SiO_2$ wafer was performed. The $SiO_2$ layer used as insulating layer was thermally grown on the silicon wafer to a thickness of 220-230 nm. The results demonstrated that the contact angle decreased from $100^{\circ}$ to $80^{\circ}$ at 28 V applied potential on $SiO_2$ wafer coated with OTS and the contact angle appeared to be reversible. However, the contact angle on the $SiO_2$ wafer coated with Z- Tetraol 2000 was not observable at 28 V applied potential. Furthermore, the contact angle on the Si wafer coated with OTS or Z- Tetraol 2000 appeared to be irreversible due to the generation of electrolysis in the droplet. It is concluded that it is feasible to use SAM as a hydrophobic layer in electrowetting applications.