• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3369-3375
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• 2016

MicroRNAs, a novel class of small non-coding RNAs, are key players in many cellular processes, including cell proliferation, differentiation, invasion and regeneration. Tissue and circulatory microRNAs could serve as useful clinical biomarkers and deregulated expression levels have been observed in various cancers. Gene variants may alter microRNA processing and maturation. Thus, we aimed to investigate the association of MIR-196a2 rs11614913 (C/T), MIR-499a rs3746444 (A/G) polymorphisms and their combination with cancer susceptibility in an Egyptian population. Sixty five renal cell carcinoma (RCC) and 60 hepatocellular carcinoma (HCC) patients and 150 controls were enrolled in the study. They were genotyped using real-time polymerase chain reaction technology. Both $miR-196a2^*T$ and $miR-499a*G$ were associated with RCC risk, but only $miR-196a^*T$ was associated with HCC development. Carriage of the homozygote combinations ($MIR196a2^*TT+MIR499a^*AA$) and ($MIR196a2^*CC+MIR499a^*GG$) was associated with 25 and 48 fold elevation of likelhood to develop RCC, respectively. The miR-196a2 SNP was also linked with larger tumor size in RCC and advanced tumor stage in HCC. miR-196a2 and miR-499a combined genotypes were associated with RCC and HCC. Further functional analysis of SNPs is required to confirm relationships between genotypes and phenotypes.

• 공기청정기술
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• 제30권1호
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• pp.57-68
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• 2017

• 한국자기학회지
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• 제8권1호
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• pp.21-26
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• 1998

온도에 따른 비정질 금속 Co66Fe4Ni1B14Si15의 자기 임피던스의 변화를 저온 장치를 써서 시료의 온도를 10K∼300K까지 변화시키면서 측정하였다. 이때, 시료에 겉어준 교류전류를 10mA로 고정시키고 주파수를 100KHz에서 10MHz 까지 변화시켰다. MIR (Magneto-Impedance Ratio)은 온도가 증가함에 따라 급격히 증가하였으며, MIR(T)≡MIR(0)exp(cT2)의 관계식으로 나타났다. 상온에서 측정한 MIR 값은 측정 주파수내에서 10K에서의 값보다 2∼3배 크게 나타났다. 이 결과는 MI 온도 효과를 이용한 온도 센서로서의 가능성을 제시한다. MIR 곡선의 모양은 온도의 증가에 따라 큰 변화가 없었으며 MIR은 주파수 증가에 따라 커지면서 MIR 곡선의 모양이 퍼지는 전형적인 경향을 나타냈다.

• The Korean Journal of Physiology and Pharmacology
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• 제23권6호
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• pp.449-458
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• 2019

Retinoblastoma (Rb) is one of the most common eye malignancies occur in childhood. The crucial roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been widely reported in Rb progression. In the present study, we found the expression of lncRNA T-cell leukemia/lymphoma 6 (TCL6) was significantly downregulated in Rb tissues and cell lines. Knockdown of lncRNA TCL6 promoted cell proliferation while reduced cell apoptosis in Rb cells. Moreover, lncRNA TCL6 serves as a sponge for miR-21, a previously-reported oncogenic miRNA in Rb, by direct targeting to negatively regulated miR-21 expression, therefore modulating Rb proliferation through miR-21. TCL6 overexpression inhibited Rb cell proliferation while miR-21 overexpression exerted an opposing effect; the effect of TCL6 overexpression was partially attenuated by miR-21 overexpression. PTEN/PI3K/AKT signaling pathway was involved in lncRNA TCL6/miR-21 axis modulating Rb cell proliferation. Taken together, lncRNA TCL6 serves as a tumor suppressor by acting as a sponge for miR-21 to counteract miR-21-mediated PTEN repression.

• Korean Journal of Mathematics
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• 제10권1호
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• pp.37-44
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• 2002

In this paper, we obtain an algebraic structure which is equivalent to an MV-algebra. Moreover, we show that $t$-norm and $t$-conorm can be obtained from MV-algebras.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제5권2호
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• pp.140-144
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• 2005

We investigate the P-generating functions, L-generating functions, and A-generating function, respectively induced by product t-norms, Lukasiewicz t-norms and additive semi-groups. Furthermore, we investigate the relations among them.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3961-3968
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• 2015

Background: Variants of human papillomavirus (HPV) show more oncogenicity than do prototypes. The HPV16 Asian variant (HPV16As) plays a major role in cervical cancer of Asian populations. Some amino acid changes in the E6 protein of HPV16 variants affect E6 functions such as p53 interaction and host immune surveillance. This study aimed to investigate activities of HPV16As E6 protein on modulation of expression of miRNA-21 as well as interferon regulatory factors (IRFs) 1, 3, 7 and c-fos. Materials and Methods: Vectors expressing E6 protein of HPV16As (E6D25E) or HPV16 prototype (E6Pro) were constructed and transfected into C33A cells. HCK1T cells expressing E6D25E or E6Pro were established by transducing retrovirus-containing E6D25E or 16E6Pro. The E6AP-binding activity of E6 and proliferation of the transfected C33A cells were determined. MiR-21 and mRNA of interesting genes were detected in the transfected C33A cells and/or the HCK1T cells, with or without treatment by culture medium from HeLa cells (HeLa-CM). Results: E6D25E showed binding activity with E6AP similar to that of E6Pro. Interestingly, E6D25E showed a higher activity of miR-21 induction than did E6Pro in C33A cells expressing E6 protein. This result was similar to the HCK1T cells expressing E6 protein, with HeLa-CM treatment. The miR-21 up-regulation significantly corresponded to its target expression. Different levels of expression of IRFs were also observed in the HCK1T cells expressing E6 protein. Interestingly, when treated with HeLa-CM, IRFs 1, 3 and 7 as well as c-fos were significantly suppressed in the HCK1T cells expressing E6D25E, whereas those in the HCK1T cells expressing E6Pro were induced. A similar situation was seen for IFN-${\alpha}$ and IFN-${\beta}$. Conclusions: E6D25E of the HPV16As variant differed from the E6 prototype in its activities on epigenetic modulation and immune surveillance and this might be a key factor for the important role of this variant in cervical cancer progression.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6601-6604
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• 2013

Aim: Associations between polymorphisms in miR-146aG>C, miR-196a2C>T and miR-499A>G and risk of HCC, and interaction with HBV infection in a Chinese population, were the target of the present research. Methods: The duplex polymerase-chain-reaction with confronting-two-pair primers (PCR-RFLP) was performed to determine the genotypes of the miR-146aG>C, miR-196a2C>T and miR-499A>G genotypes. Associations of polymorphisms with the risk of HCC were estimated by conditional logistic regression analysis. Results: Drinking, family history of cancer, HBsAg and HCV were risk factors for HCC. Multivariate regression analyses showed that subjects carrying the miR-196a2 CC genotype had significantly increased risk of HCC, with an adjusted OR (95% CI) of 2.18 (1.23-3.80). In addition, cases carrying the miR-196a2 C allele had a 1.64-fold increase in the risk for HCC (95%CI=1.03-2.49). The miR-196a2 CT and TT genotypes greatly significantly increased the risk of HCC in subjects with HBV infection, with adjusted ORs (95% CI) of 2.02 (1.12-3.68) and 2.69 (1.28-5.71), respectively. Conclusion: Our results demonstrate that miR-196a2 CC genotype and C allele have an important role in HCC risk in Chinese, especially in patients with HBV infection.

• 한국의료질향상학회지
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• 제23권2호
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• pp.57-66
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• 2017

Purpose:The purpose of this study was to identify the patient's characteristics influencing patient's comprehensibleness of doctor's explanation. Methods: This study was conducted as a secondary data analysis using the raw data of The 6th Korea National Health and Nutrition Examination Survey (KNHANES VI) conducted in 2015. The survey sampled 22,948 adults, and 4,469 of them were included in this analysis. Multiple regression analysis was performed to determine the patient's characteristics influencing patient's comprehensibleness of doctor's explanation. Results: The patient's comprehensibleness of doctor's explanation were more higher as age (t=5.65, p<.001), female (t=4.40, p<.001), subjective good health status (t=3.48, p=.001) were higher. On the other hand, the patient's comprehensibleness of doctor's explanation were more higher education level (t=-6.80, p<.001), not-recent outpatient experience (t=-6.04, p<.001), pain/discomfort (t=-2.64, p=.008), anxiety/depression (t=-2.58, p=.010) were lower. Conclusion:It is necessary to be provided the patients who are higher education level, pain/discomfort and anxiety/depression with applying the doctor's detailed explanation and intervention programs.

• 충청수학회지
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• 제17권2호
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• pp.197-203
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• 2004

We find some properties of a stochastic processX(t, ${\omega}$) ${\in}L^2_{s.a.p.}$ which is of bounded variation.