• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.116-121
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• 2010

Long-chain polyunsaturated fatty acids (LC-PUFA) promote the development of brain and vision of the fetus, relieve inflammation, inhibit oral dysplasia of rumor cell, decrease the incidence of cardiovascular disease and regulate arrhythmia. ${\Delta}-6$ desaturase is the rate-limited enzyme in the desaturation process. This study reports the cloning, characterization and tissue expression of a ${\Delta}-6$ desaturase gene in the chicken. PCR primers were designed based on the predicted sequence of chicken ${\Delta}-6$ desaturase (accession number: XM421053) and used to isolate a cDNA fragment of 1,323 bp from chicken liver. Based on the 1,323 bp fragment an EST (BI390105) was obtained by BLAST. The EST and 5'nd of the 1,323 bp fragment were partially overlapped. Gene specific primers derived from the EST were used for amplification of the 5'nd. Another gene-specific primer derived from the 1,323 bp fragment was used for amplification of the 3'nd by 3'ACE. Then the three overlapping cDNA sequences obtained were assembled with DNAMAN software and a full-length ${\Delta}-6$ desaturase of 2,153 bp was obtained. The full-length cDNA contained an ORF of 1,335 bp with a 5'ntranslated region of 147 nucleotides followed by an ATG initiation codon. Stop codon TGA was at position 1,481-1,483 bp. The deduced amino acids shared an homology above 77% with bovine, mice, orangutan, rat and human. The protein sequence had three histidine-rich regions HDFGH (HisI region), HFQHH (HisII region) and HH (HisIII region), a cytochrome $b_{5}$-like domain containing a heme-binding motif and two transmembrane domains. Sequence analysis of the chicken genomic DNA revealed that the coding sequence of chicken ${\Delta}-6$ desaturase included 12 exons and 11 introns. Semi-quantitative RT-PCR showed that the ${\Delta}-6$ desaturase expression levels were in turn liver, spleen, pancreas, lung, breast muscle, heart, and abdominal fat. The expression of ${\Delta}-6$ desaturase in liver was significantly higher than that in breast muscle (p<0.01). The expression of ${\Delta}-6$ desaturase in lung was significantly higher than that in abdominal fat (p<0.01). This is the first clone of chicken ${\Delta}-6$ desaturase.

• Journal of Aquaculture
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• v.18 no.4
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• pp.272-279
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• 2005

The efficacy of lidocaine hydrochloride and Clove oil as anaesthetics was evaluated in the Korean rose bitterling, Rhodeus uyekii (Mori, 1935) and oily bitterling, Acheilognathus koreensis (Kim and Kim, 1990) at four different temperatures of $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$. When complete anaesthesia was acquired less than 3 min and recovery was acquired less than 10 min, the optimal dose range of lidocain hydrochloride at $20^{\circ}C$ was 250${\~}$550 ppm in Korean rose bitterling, and 150${\~}$550 ppm in oily bitterling, respectively. In case of Clove oil, the optimal dose range at $20^{\circ}C$ was 40${\~}$200 ppm in Korean rose bitterling and 80${\~}$240 ppm in oily bitterling, respectively. Both of lidocaine hydrochloride and Clove oil resulted in a negatively dose-dependent manner for anaesthesia induction time in these two species. Recovery times were more variable in relation to anaesthetic doses, but in general higher anaesthetic doses resulted in similar or longer recovery time. As expected, the lower temperature resulted in longer anaesthesia induction and recovery time. The study demonstrated that lidocaine hydrochloride and Clove oil can be used as effective anaesthetics in these two species. The results from this study could be useful for aquaculturists industry and other related husbandry practices that require anaesthesia of Korean rose bitterling and oily bitterling.

• Culinary science and hospitality research
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• v.11 no.4 s.27
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• pp.77-91
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• 2005

The aim of this study was to determine what kinds of an effect on dietary behaviors of elementary school students in seoul by gender. So this survey is classified into nutrition knowledge, food habit dietary behaviors. Nutrition knowledge is different from gender, male is scoring 6.90 out of 10 and female is scoring 7.03 out of 10. Food habit is also different from gender, male is scoring 4.17 out of 10 and female is scoring 4.79 out of 10. Dietary behaviors also female is better than male. This result shows that food service should be more highly satisfied with the food service quality than the food service quantity.

• Culinary science and hospitality research
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• v.18 no.2
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• pp.34-50
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• 2012

This study conducted the empirical analysis of the influence that are quality attributes, and strengths, weaknesses, low ranking and excess of Korean restaurants menu on revisit intention based on differences between importance and satisfaction by surveying Korean students in Paris, and also conducting IPA analysis based on survey result. The result of IPA analysis showed weaknesses that are the visual elements of foods; in contrast it showed strengths that are the price, temperature, taste, quantity, cleanliness, freshness, and flavor of dishes. Also it showed low ranking that are seasonal items, authenticity, originality, sizes, colors, texture, explanations, ingredients, recipes of menu, classification of healthy food, suitability of bowls, creativity of existing food. Therefore, it is found that the higher recognition of attributes, organic functions, properties, and explanations of menu, the higher revisit intention. It showed that explanations, sensibility, characters, quality of menu influenced on revisit intention according priority by analysis of quality attributes of menu on revisit intention. This study is significant in that it provides useful data services for marketing strategies and operational suggestions to globalization of Korean food by analysis local menu of Korean food service industry.

• Culinary science and hospitality research
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• v.15 no.2
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• pp.17-30
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• 2009

The purpose of this study was to investigate awareness and knowledge on Korean traditional food among the residents in Gyeongju and Pohang area and develop and maintain it. 452 copies of a questionnaire were surveyed and analyzed statistically. The research findings were as follows. Firstly, males prefer a la Carte menu while females prefer kimchi, bab(boiled rice) juk(rice-gruel), guksu(noodles) and mandu(dumplings). Secondly, the residents favor kimchi, bulgogi and bibimbab with assorted mixtures. Thirdly, comparing the positive perception of Korean traditional food between males and females, females were more aware of traditional food and informed than males. Lastly, correspondence analysis showed that thirties prefer bab, tang(soup) and guksu while forties gu-i(roast) & jeon(panfried food) with seasonings and fifties kimchi & jang(sauce and paste). Also, high school graduates prefer eumcheong & han-gwa while college graduates bab, tang, and guksu. The residents whose monthly incomes are under W1,000,000 favor bab, tang and guksu, and those who earn over W5,000,000 per month favor kimchi & jang. The higher educational levels and incomes were, the higher qualitative factors were evaluated. Based on those findings, Korean traditional food should be generalized and more kinds of its menu should be developed.

• Journal of Digital Convergence
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• v.13 no.12
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• pp.337-346
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• 2015

Purpose: The purpose of this study is to identify the effect of a convergence multicultural nursing education on cultural competence and empathy for nursing students. Method: This used a total of 85 samples with an experimental group and a control group and pre-and post-testing. The samples comprised of 85 third-year nursing students enrolled in the BSN program were divided into an experimental group (n=45) from college J and an control group (n=40) from college K. Results: The experimental group of students who attended a convergence multicultural nursing education program increased the level of cultural competence and empathy significantly. But the control group of students who did not receive multicultural nursing education program had no significant increase. Conclusion: The study proves that a convergence multicultural nursing education program has a positive impact on the improvement of cultural competence and empathy for nursing students.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.6
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• pp.122-130
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• 2013

The demand and the supply are increasing sharply in accordance with the growth of the Memory Semiconductor Industry. The Flash Memory above all is being utilized substantially in the Industry of smart phone, the tablet PC and the System on Chip (SoC). The Flash Memory is divided into the NOR-type Flash Memory and the NAND-type Flash Memory. A lot of study such as the Built-In Self Test (BIST), the Built-In Self Repair (BISR) and the Built-In Redundancy Analysis (BIRA), etc. has been progressed in the NOR-type fash Memory, the study for the Built-In Self Test of the NAND-type Flash Memory has not been progressed. At present, the pattern test of the NAND-type Flash Memory is being carried out using the outside test equipment of high price. The NAND-type Flash Memory is being depended on the outside equipment as there is no Built-In Self Test since the erasure of block unit, the reading and writing of page unit are possible in the NAND-type Flash Memory. The Built-In Self Test equipped with 2 kinds of finite state machine based structure is proposed, so as to carry out the pattern test without the outside pattern test equipment from the NAND-type Flash Memory which carried out the test dependant on the outside pattern test equipment of high price.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.16 no.2
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• pp.82-87
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• 2006

Glassy specimen was obtained by melting and quenching a borosilicate glass frit miked with $10{\sim}80wt%$ EAF dust. The glass transition temperature, $T_g$ of glassy specimen was measured around $550^{\circ}C$ from the DTA curve and the eat treatment condition to crystallize a glassy specimen was selected as $700^{\circ}C$/1 hr. The spinel crystal peaks were found in XRD analysis for the glass containing dust > 70 wt%. For the glass-ceramics, however, the spinel peaks in a specimen containing dust > 40 wt%, and the spinel and willemite peaks in dust > 80 wt%. The crystals of several tens of nanometer with aspect ratio of $0.7{\sim}1.0$ were observed at a glassy specimen containing dust > 40 wt% by SEM which was not found by XRD analysis. For the glass-ceramics, the crystals were found in a specimen containing dust of even 10 wt% by SEM. The feature and distribution of crystals observed through the specimen for a glass-ceramics were diverse and not uniform. The crystals found in the glass-ceramics containing 70 wt% dust were hexahedral with an aspect ratio of around unity.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.16 no.2
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• pp.88-93
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• 2006

To recycle the electric arc furnace (EAF) dust as a ceramic raw materials, the leaching concentrations of heavy metals (Zn, Pb, Cr, Cd) were analysed with various pH and mixing ratios for EAF dust and EAF dust-clay mixtures. The evaporation amounts of the some heavy metals were evaluated by measuring their total concentrations in the sintered bodies of EAF dust-clay mixtures with various mixing ratio and sintering temperature. Toxicity characteristic leaching procedure (TCLP) test was conducted for evaluating the chemical stabilities of the heavy metal elements. Leaching concentrations of heavy metal ions shows minimum leaching concentration at the pH 10. Evaporation amount of heavy metals in the sintered bodies were evaluated for the mixtures of pH 10 depenidng on mixing ratio and sintering temperature. Evaporation of heavy metal components were increased with increasing the sintering temperature and contents of the EAF dust. The evaporation of the heavy metal components in EAF dust was effectively suppressed by increasing the clay content. The leaching concentrations of heavy metal components were decreased with increasing clay content and temperature.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.9
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• pp.1552-1559
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• 2004

In order to evaluate the potential utilization value as a novel probiotic strain, the immunopotentiating activities of the cellular components from Lactobacillus brevis FSB-1 were examined. L. brevis FSB-1 isolated from kimchi were fractionated into the whole cell, cell wall, cytosol and extracellular preparation, and each fraction was examined on intestinal immune system modulating activity in vitro. The cell wall and cytosol preparation showed the relatively high bone marrow cell proliferating activity through Peyer's patch cell in a dose-dependent manner. But these preparations did not directly stimulate the bone marrow cell proliferation. The whole cell, cell wall and cytosol preparation also induced considerable levels of macrophage activation and mitogenicity of murine splenocytes in vitro. The anti-complementary activity (ITCH_(50)) of the cytosol fraction of L. brevis FSB-1 was the most potent in the cellular components, and the activity showed dose dependency. The complement activation by the cytosol fraction of L. brevis FSB-1 occurs via both alternative and classical pathways, which confirmed by the crossed immunoelectrophoresis using anti-human C3.