• 말소리와 음성과학
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• 제9권2호
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• pp.9-22
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• 2017

The purpose of this paper is to report the whole process of developing Korean Standard Speech Database (KSS DB). This project is supported by SPO (Supreme Prosecutors' Office) research grant for three years from 2014 to 2016. KSS DB is designed to provide speech data for acoustic-phonetic and phonological studies and speaker recognition system. For the samples to represent the spoken Korean, sociolinguistic factors, such as region (9 regional dialects), age (5 age groups over 20) and gender (male and female) were considered. The goal of the project is to collect over 3,000 male and female speakers of nine regional dialects and five age groups employing direct and indirect methods. Speech samples of 3,191 speakers (2,829 speakers and 362 speakers using direct and indirect methods, respectively) are collected and databased. KSS DB designs to collect read and spontaneous speech samples from each speaker carrying out 5 speech tasks: three (pseudo-)spontaneous speech tasks (producing prolonged simple vowels, 28 blanked sentences and spontaneous talk) and two read speech tasks (reading 55 phonetically and phonologically rich sentences and reading three short passages). KSS DB includes a 16-bit, 44.1kHz speech waveform file and a orthographic file for each speech task.

• 대한수의학회지
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• 제36권3호
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• pp.665-680
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• 1996

Bovine Pneumonic Pasteurellosis는 수송열(輸送熱)로 일반적으로 알려져 있는 질병으로서, 여러가지 요인의 복합적(複合的)인 작용에 의해 발병하는 것으로 알려져 있으나, Pasteurella haemolytica A1이 가장 주요(主要)한 인자(因子)로 밝혀져 있다. P haemolytica A1은 leukotoxin(LKT), lipopolysaccharide(LPS), capsular polysaccharide 등 여러가지의 병원성인자(病原性因子)을 생성한다. 이들 인자중 LKT가 가장 중요한 병원성인자로 밝혀져 있다. 이에 본 실험은 P haemolytical A1의 LKT 유전자를 Bacillus subtilis에서 발현(發現)시킴으로서 LPS에 오염(汚染)되지 않은 LKT을 대량으로 생산할 목적으로 실시되었다. 실험의 첫 단계(段階)로서 pLKT52 plasmid을 Sau3 A1의 제한효소을 이용하여 부분소화(部分消化)시킨 후 이 부분 소화(消化)된 유전자들로부터 3~5kb 크기의 유전자들을 순수분리하여 pUC18와 결합시킨 후 E coli NM522에 형질전환(形質轉換)시켰다. 이때 형질전환된 균주들은 LKT에 대한 단크론 항체인 MAb601을 이용하여 colony blot 법에 의해서 LKT 유전자 보유 및 발현여부(發現與否)을 조사하였다. 이들 양성 clone들은 제한효소분석(制限酵素分析), 염기서열분석(鹽基序列分析) 및 Western blot 등에 의해서 재확인(再確認)하였다. 총 9개의 양성 clone중 위의 방법에 의해서 한 clone을 선택(選擇)하여 lktCA insert를 재분리하여 shuttle vector에 subcloning 하였다. Subcloning된 LKT 유전자들은 shuttle vector의 종류(種類)(pHPS9, p602/20, pHPS9-Sac)와 각기(各其) 다른 종류(種類)의 B subtilis(spoO12A, BR121, WB3O, Raj1105) 숙주내(宿主內)에서 발현정도를 Western blot 법에 의해서 비교(比較)하였다. 이때 최적발현조건(最適發現條件)은 p602/20와 pBL1의 dual plasmid system을 이용하여 B subtilis spoO12A에서 2시간동안 IPTG로 발현을 유도(誘導)하는 것이었다. B subtilis에서 발현된 LKT을 visual 법과 neutral red uptake 법을 이용하여 소 폐포(肺胞) 대식구(大食求)에 대한 biological activity를 확인하였다. 발현된 LKT에 대한 LPS 오염은 LKT을 SDS-PAGE 후 silver stain에 의해서 확인하였다. 본 실험을 통해서 볼 때에 lktCA 유전자를 보유(保有)하고 있는 p602/20는 B subtilis에서 매우 불안정(不安定)하였고, 발현된 LKT는 세균자체(細菌自體)에서 생성되는 protease들에 의해서 파괴(破壞)됨으로서 농도(濃度)가 매우 낮았다. 이러한 문제점들은 다음 단계(段階)의 실험에서 해결되어야할 문제들이다.

• 대한수의학회지
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• 제20권2호
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• pp.159-165
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• 1980

A total of 98 strains of Bordetella bronckiseptica isolated from pigs affected the infectious atrophic rhinitis(AR) during 1978 were surveyed for drug sensitivity to 26 chemotherapeutic agents, and minimum inhibitory concentration(MIC), incidence rate of resistant strain and resistant patern from the strains which were obtained from the different pig farm in Jeonnam province were examined. The results obtained are summarized as follows. 1. Most of the strains tested were resistant to Ampicillin (AB, PC), spiramycin(SPO, sulfa drugs (SD) (MIC:$400.0{{\mu}g/ml}$) and streptomycin(SM) (MIC:$200.0{{\mu}g/ml}$). Of the 75.0% of strains were also resistant to penicillin(PC) (MIC:$200.0{{\mu}g/ml}$) and of the 14.3 of strains were inhibited to grow to tetracycline(TC), chlortetracycline(CTC), oxytetracyc-line(OTC), erythromycin(EM), tylosin(TS), leucomycin (LM) and chloramphenicol (CP) (MIC:$6.25{{\mu}g/ml}$). On the other hand, most of the strains tested were inhibited to grow to kanamycin(KM), gentamycin(GM) neomycin(NM) (MIC:$25.0{{\mu}g/ml}$) and to colistin(CL) (MIC:$12.5{{\mu}g/ml}$). 2. Incidence rate of resistant strains to main chemotherapeutic agents was 100.0% of sulfa drugs, 96.4% of streptomycin, 85.7% of penicillin, tetracycline, chloramphenicol and erythromycin, 46.4% of gentamycin, 17.9% of colistin and 0.0% of kanamycin and nalidixic acid.

• 대한안전경영과학회지
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• 제12권4호
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• pp.81-86
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• 2010

Despite the importance of cycling postures during cycling performances, there has been a very little research investigating cycling postures and pedaling rate for particularly concerning domestic cyclists. The aim of this study was to analyze correlations and effects between cycling postures and pedaling rate in track cycling. Twelve male racing cyclists (six racing and university cyclists) participated in this research. For this study, seven infrared cameras (Qualisys ProReflex MCU-240s) were used for collecting data and these were processed via QTM (Qualisys Tracker Manager) software. It appeared that pedaling rate had correlations with regard to a shoulder angle (R=-.601) and displacement between shoulder joints(R= -.637), but a knee (R=-.601) and ankle angle (R=.667). Moreover, two multiple regression equations of pedaling rate for cycling postures were significant and R2 of the first order equation y (pedaling rate) = 0.039x (knee angle) - 1.068 was less than the second order equation y = 0.006x2 - 1.287x + 69.674. In conclusion, cycling postures affected the pedaling rate. Further study should be researched on postures in relation to air resistance in a wind tunnel.

• International Journal of Industrial Entomology and Biomaterials
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• 제28권2호
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• pp.25-31
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• 2014

Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvae of P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, and bioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as the insect pathogen Serratia marcescens. Therefore, we highlight the use of this insect as an alternate food and the need for its sanitary rearing conditions, as contamination may affect public health.

• 한국산업정보학회논문지
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• 제20권3호
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• pp.87-93
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• 2015

졸음 운전 등 운전자의 상태 변화에 따른 자동차 사고가 급증하고 있으며 이를 방지하기 위한 시스템 구축 및 운전자의 상태를 판단하는 알고리즘 개발이 요구되어 지고 있다. 본 논문에서는 모의 주행 시스템을 통한 운전자의 심박변이도, 산호 포화도 (SPO2), 체온을 측정하여 운전자의 상태를 알려 주는 실험을 수행하였다. 즉, 심박변이도 (Heart rate variability, HRV) 분석을 위해 운전자의 심전도(Electrocardiogram, ECG) 신호를 획득 한 후 심전도 P,QRS, T 파형 중 R peak 을 자동으로 검출하였고 이를 통해 구한 R-R interval을 이용하여 HRV의 주요 파라메타를 시간영역(time domain)으로 해석하여 작업 환경에 따른 운전자의 상태를 비교 분석하였다.

• 환경생물
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• 제22권4호
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• pp.487-493
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• 2004

To investigate the antifungal activity related protein in pesticidal bacteria, a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province, Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 168 rDNA sequencing. It has an antifungal activity against 9 plant pathogenic fungi in a paper disc assay. The antifungal activity- deficient mutant, B. subtilis mLTD was induced at a 5 kGy dose of $^{60}Co$ gamma radiation. Using the two-dimensional electrophoresis and the matrix assisted laser desorption ionization time-of-flight mass spectrometry, the comparison analysis of proteins between the wild and mutant were performed. A major intracellular serine proteinase IspA (MW: 32.5 kDa), a NAD (P) H dehydrogenase (MW: 20.0 kDa), and a stage II sporulation protein AA, SpoIIAA (MW: 14.3kDa) were detected only in the B. subtilis LTD. These results suggested that the functions of these proteins found only in the B. subtilis LTD could. be closely related to the antifungal activity against plant pathogenic fungi.

• Structural Engineering and Mechanics
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• 제68권6호
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• pp.647-660
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• 2018

The Incremental Dynamic Analysis (IDA) procedure is currently known as a robust tool for estimation of seismic collapse capacity. However, the procedure is time-consuming and requires significant computational efforts. Recently some simplified methods have been developed for rapid estimation of seismic collapse capacity using pushover analysis. However, a comparative review and assessment of these methods is necessary to point out their relative advantages and shortcomings, and to pave the way for their practical use. In this paper, four simplified pushover analysis-based methods are selected and applied on four regular RC intermediate moment-resisting frames with 3, 6, 9 and 12 stories. The accuracy and performance of the different simplified methods in estimating the median seismic collapse capacity are evaluated through comparisons with the results obtained from IDAs. The results show that reliable estimations of the summarized 50% fractile IDA curve are produced using SPO2IDA and MPA-based IDA methods; however, the accuracy of the results for 16% and 84% fractiles is relatively low. The method proposed by Shafei et al. appears to be the most simple and straightforward method which gives rise to good estimates of the median sidesway collapse capacity with minimum computational efforts.

• 대한임베디드공학회논문지
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• 제17권1호
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• pp.19-24
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• 2022

Through the development and dissemination of embedded devices, studies that may help patients are rapidly emerging. Recently, as wearable devices have become one of the ways to diagnose diseases in daily life, they are being studied as a way to assist severely ill patients to lead their daily lives. Among them, a method of detecting and giving signals to detect and solve symptoms using acceleration sensors to diagnose Parkinson's disease is being studied, and there is no study to measure and analyze various factors that can affect Parkinson's disease. To solve them, we designed and developed a wearable device, P-Band, with various sensors capable of diagnosing related symptoms, including acceleration sensors capable of diagnosing Parkinson's disease. In this paper, the overall structure of the P-Band and the description and operation method of the measurable sensors are presented. In addition, it was confirmed that the symptoms of Parkinson's patients could be determined complexly through the results measured in actual patients.

• Laboraroty Animal Research
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• 제34권4호
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• pp.270-278
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• 2018

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, $Avertin^{(R)}$) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak $CO_2$ ($ETCO_2$), mean arterial blood pressure, heart rate, oxygen saturation ($SpO_2$), body temperature, pH, $PCO_2$, and $PO_2$ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, $SPO_2$, arterial blood $PCO_2$, and $PO_2$, which decreased after TBE anesthesia, or in heart rate and $ETCO_2$, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.