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http://dx.doi.org/10.7852/ijie.2014.28.2.25

Detection of Insect Pathogen Serratia marcescens in Protaetia brevitarsis seulensis (Kolbe) from Korea  

Kwak, Kyu-Won (Department of Agricultural Biology, National Academy of Agricultural Science)
Han, Myung-Sae (Department of Bio-fibers and Materials Science, Kyungpook National University)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science)
Choi, Ji-Young (Department of Agricultural Biology, National Academy of Agricultural Science)
Lee, Seok-Hyun (Department of Agricultural Biology, National Academy of Agricultural Science)
Choi, Young-Cheol (Department of Agricultural Biology, National Academy of Agricultural Science)
Park, Kwan-Ho (Department of Agricultural Biology, National Academy of Agricultural Science)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.28, no.2, 2014 , pp. 25-31 More about this Journal
Abstract
Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvae of P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, and bioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as the insect pathogen Serratia marcescens. Therefore, we highlight the use of this insect as an alternate food and the need for its sanitary rearing conditions, as contamination may affect public health.
Keywords
Protaetia brevitarsis seulensis (Kolbe); Serratia marcescens; 16SrRNA; PCR; Electromicroscope; Bioassay;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Schwarz RHaH (2004) Serratia marcescens internalization and replication in human bladder epithelial cells. BMC Infect Dis 4
2 Mulcahy H, Sibley CD, Surette MG, Lewenza S (2011) Drosophila melanogaster as an animal model for the study of Pseudomonas aeruginosa biofilm infections in vivo. PLoS Pathol 7(10):e1002299 doi:10.1371/journal.ppat.1002299   DOI
3 Murdoch SL, Trunk K, English G, Fritsch MJ, Pourkarimi E, Coulthurst SJ (2011) The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol 193(21):6057-69 doi:10.1128/JB.05671-11   DOI   ScienceOn
4 Nunez-Valdez ME, et al. (2008) Identification of a putative Mexican strain of Serratia entomophila pathogenic against root-damaging larvae of Scarabaeidae (Coleoptera). Appl & Environ Microbiol 74(3):802-10 doi:10.1128/AEM.01074-07   DOI   ScienceOn
5 Suh HJ, Kim SR, Lee KS, Park S, Kang SC (2010) Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda:Insecta) larvae. J Photochem Photobiol B. 99(2):67-73 doi:10.1016/j.jphotobiol.2010.02.005   DOI   ScienceOn
6 Tanada Y, Kaya HK (1994) Insect Pathology. Academic Press INC. Harcourt Brace Jovanovich, Pubrichers, San Diego. [Internet]. Available from: http://wiki.ceh.ac.uk [accessed on 27 October 2013].
7 White G.F. (1923). Hornworm septicemia. J Agri Res 26:447-486
8 Joyner J, Wanless D, Sinigalliano CD, Lipp EK (2014) Use of Quantitative Real-Time PCR for Direct Detection of Serratia marcescens in Marine and Other Aquatic Environments. Appl Environ Microbiol 80(5):1679-83 doi:10.1128/AEM.02755-13   DOI   ScienceOn
9 Jung J-A, et al. (2013) Specific Detection of Serratia marcescens Based on a PCR Assay and Antimicrobial Susceptibility of S. marcescens Isolated from Boar Semen. J Life Sci 23(9):1133-1139 doi:10.5352/jls.2013.23.9.1133   과학기술학회마을   DOI   ScienceOn
10 Kwon E-Y, et al. (2013) Pre-treatment of the White-Spotted Flower Chafer (Protaetia brevitarsis) as an Ingredient for Novel Foods. Korean J Soc Food Sci & Nut 42(3):397-402 doi:10.3746/jkfn.2013.42.3.397   과학기술학회마을   DOI   ScienceOn
11 Fedrigo GV, Campoy EM, Di Venanzio G, Colombo MI, Garcia Vescovi E (2011) Serratia marcescens is able to survive and proliferate in autophagic-like vacuoles inside non-phagocytic cells. PloS one 6(8):e24054 doi:10.1371/journal.pone.0024054   DOI
12 Lauri Mikonranta JM, Minna Kaukoniitty and Dalial Freitak (2014) Insect immunity oral exposure to a bacterial pathogen elicits free radical response and protects from a recurring infection. Front Zool 11(23):1-7   DOI   ScienceOn
13 Martinez-Granero F, et al. (2014) Identification of flgZ as a Flagellar Gene Encoding a PilZ Domain Protein That Regulates Swimming Motility and Biofilm Formation in Pseudomonas. PloS one 9(2):e87608 doi:10.1371/journal.pone.0087608   DOI
14 Miyanoshita A, et al. (1996) Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma. Biochem & Biophysic Res Comm 220(3):526-31 doi:10.1006/bbrc.1996.0438   DOI   ScienceOn
15 Gerc AJ, Song L, Challis GL, Stanley-Wall NR, Coulthurst SJ (2012) The insect pathogen Serratia marcescens Db10 uses a hybrid nonribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin. PloS one 7(9):e44673 doi:10.1371/journal.pone.0044673   DOI
16 Grimont F, Grimont PAD (2006) The Genus Serratia.219-244 doi:10.1007/0-387-30746-x_11   DOI
17 Hurst MR, Beard SS, Jackson TA, Jones SM (2007a) Isolation and characterization of the Serratia entomophila antifeeding prophage. FEMS Microbiol Lett 270(1):42-8 doi:10.1111/j.1574-6968.2007.00645.x   DOI   ScienceOn
18 Carol R. Lauzon TGB, Robert E. Sjogren and Ronald J. Prokopy (2003) Serratia marcescens as a bacterial pathogen of rhagoletis pomonella flies. Eur J Entomol 100:87-92   DOI
19 Hurst MR, Jones SM, Tan B, Jackson TA (2007b) Induced expression of the Serratia entomophila Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra zealandica. FEMS Microbiol Lett 275(1):160-7 doi:10.1111/j.1574-6968.2007.00886.x   DOI   ScienceOn
20 Jang-Jih Lu C-LP, Shih-Yi Lee and Chih-Chieng Wan (2000) Use of pcr with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid. J Clin Microbiol 38(6):2076-2080
21 Ajithkumar B (2003) Spore-forming Serratia marcescens subsp. sakuensis subsp. nov., isolated from a domestic wastewater treatment tank. Int J Syst Evol Microbiol 53(1):253-258 doi:10.1099/ijs.0.02158-0   DOI   ScienceOn
22 Arthur I. Aronson Wb, and Peter dunn (1986) Bacillus thuringiensis and related insect pathogens. Microbiol Rev:1-24
23 Burnside CE (1928) A Septicemic condition of adult bees. J Econ Entomol 21, 379-386   DOI
24 Casper Flyg KKaJGB (1980) Insect pathogenic properties of serratia marcescens phage resistant mutants witha decreased resistance to cecropia immunity and a decreased virulence to drosophila. J Gen Microbiol 120:173-181
25 El-Aasar A.M.; El-Sheikh TAA, Rafea, Heba S. and Ali S.H. (2013) Biological and biochemical effects of bacillus thuringiensis serratia marcescens and teflubenzuron and their sequential combined effects on cotton leafworm spodoptera littoralis. Egypt Acad J Biolog Sci 5(1):1-13