• 대한화학회지
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• 제46권1호
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• pp.46-51
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• 2002

$Ni(II){\cdot}Gly$-Gly-His(Arg)COOH와 $Cu(II){\cdot}Gly$-Gly-His(Arg)COOH 형태의 금속펩타이드를 이용하여 P. alcaligenes에서 얻은 5S rRNA의 구조를 조사하였다. 그 결과 금속 펩타이드들은 5S rRNA의 줄기-고리 구조에서 염기쌍을 이루지 않거나 불안정하게 이루는 부분을 선택적으로 변형시켰다. 금속펩타이드의 선택성은 중심 금속이 Ni(II)인 경우와 Cu(II)인 경우에 차이가 거의 없었다. 금속펩타이드를 이용한 절단 결과를 금속 착물 M(II)CR을 이용한 결과와 비교하면 금속펩타이드에 의한 선택성이 더 크게 나타났다. 금속펩타이드와 금속착물을 이용한 절단 결과로부터 P. alcaligenes에서 얻은 5S rRNA의 이차구조를 살펴보았다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권1호
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• pp.277-280
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• 2007

Field emission-back light unit (FE-BLU) was fabricated using carbon nanotube (CNT) emitter. Local dimming and local brightening techniques were achieved, which results in very high contrast ratio. In addition, the motion blur phenomenon, one of the serious problems of liquid crystal display (LCD) with cold cathode fluorescent lamp (CCFL)-BLU, was removed from LCD-TV by using FE-BLU.

• Computers and Concrete
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• 제1권4호
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• pp.389-400
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• 2004

The examinations carried out have confirmed a relationship existing between the character of fracture surfaces and the composition and structure of (basalt and gravel) concretes. For both concretes investigated, a very good correlation was obtained between the profile line development factor, $R_L$, and the fracture surface development factor, $R_S$. With the increase in the $R_L$ parameter, the fracture surface development factor $R_S$ also increased. Agreement between the proposed relationship of $R_S=f(R_L)$ and the proposal given by Coster and Chermant (1983) was obtained. Stereological examinations carried out along with fractographic examinations made it possible to obtain a statistical model for the determination of $R_L$ (or $R_S$) based on the volume of air voids in concrete, $V_{air}$, the specific surface of air pores, $S_V_{air}$ the specific surface of coarse aggregate, $S_{Vagg.}$, and the volume of mortar, $V_m$. An effect of coarse aggregate type on the obtained values of the profile line development factor, $R_L$, as well as on the relationship $R_S=f(R_L)$ was observed. The increment in the fracture surface development factor $R_S$ with increasing $R_L$ parameter was larger in basalt concretes than in gravel concretes, which was a consequence of the level of complexity of fractures formed, resulting chiefly from the shape of coarse aggregate grains.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제9권3호
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• pp.224-227
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• 2009

In this paper, we introduce the concept of fuzzy S-weakly r-continuous mapping on an fuzzy topological space and investigate some properties of such mappings.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2016년도 추계 학술발표논문집
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• pp.86-90
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• 2016

• Mycobiology
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• 제33권2호
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• pp.109-112
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• 2005

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

• Environmental Engineering Research
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• 제19권4호
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• pp.317-329
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• 2014

The diversity of cyanobacteria in Sri Lanka was studied in different water reservoirs, paddy fields, brackish water and tsunami affected areas using light microcopy, 16S rRNA sequences, followed by phylogenetic analysis. Based on light microscopy, 24 genera were identified from environmental samples belonging to the orders Chroococcales, Oscillatoriales, Pleurocapsales and Nostocales. In cultures, 33 genera were identified from all five cyanobacterial orders, including Stigonematales. Based on 16S rRNA gene sequences and their morphology, two isolates were identified up to species level, 72 to genus level, one isolate up to family and 11 up to order level. Twelve isolates couldn't be assigned to any taxonomic level. The results of 16S rRNA gene sequences along with the phylogenetic analysis indicated that some cyanobacterial isolates could be accommodated to genus or order level. The 16S rRNA sequence analysis data in this study confirmed that order Nostocales and order Pleurocapsales cyanobacteria are monophyletic while orders Chroococcales, Oscillatoriales and Stigonematales cyanobacteria are polyphyletic. Polyphasic approach including the combination of light microscopy, cultures and the analysis of 16S rRNA gene sequences provide a promising approach to ascertain the diversity of cyanobacteria in different habitats.

• 대한기계학회논문집
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• 제9권4호
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• pp.528-538
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• 1985

본 논문에서는 crank-rocker 운동에 의해 함수 y=sinx를 만족시키는 3차원 R-S-S-R 함수발생기구를 FDP(Fletcher-Davidon-Powell) 최적화 기법을 응용, 운동학적 으로 무오차점을 고려, 설계하려고 한다. 한편 상기와 같이 최적 설계된 기구의 실 제 운동과 요구된 운동과의 오차분석 및 운동 가능성 여부(mobility check)를 검토하 였으며 FDP 최적화 기법에 필요한 벌칙함수(penalty function)로써 Fiacco-McCormic- krhk Powell의 벌칙함수를 사용하여 그 결과를 비교하였다.

• Journal of Ecology and Environment
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• 제33권2호
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• pp.165-173
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• 2010

Soil respiration ($R_S$) is a critical component of the annual carbon balance of forests, but few studies thus far have attempted to evaluate empirical regression models in $R_S$. The principal objectives of this study were to evaluate the relationship between $R_S$ rates and soil temperature (ST) and soil water content (SWC) in soil from a cool-temperate deciduous broad-leaved forest, and to evaluate empirical regression models for the prediction of $R_S$ using ST and SWC. We have been measuring $R_S$, using an open-flow gas-exchange system with an infrared gas analyzer during the snowfree season from 1999 to 2001 at the Takayama Forest, Japan. To evaluate the empirical regression models used for the prediction of $R_S$, we compared a simple exponential regression (flux = $ae^{bt}$Eq. [1]) and two polynomial multiple-regression models (flux = $ae^{bt}{\times}({\theta}{\nu}-c){\times}(d-{\theta}{\nu})^f:$ Eq. [2] and flux = $ae^{bt}{\times}(1-(1-({\theta}{\nu}/c))^2)$: Eq. [3]) that included two variables (ST: t and SWC: ${\theta}{\nu}$) and that utilized hourly data for $R_S$. In general, daily mean $R_S$ rates were positively well-correlated with ST, but no significant correlations were observed with any significant frequency between the ST and $R_S$ rates on periods of a day based on the hourly $R_S$ data. Eq. (2) has many more site-specific parameters than Eq. (3) and resulted in some significant underestimation. The empirical regression, Eq. (3) was best explained by temporal variations, as it provided a more unbiased fit to the data compared to Eq. (2). The Eq. (3) (ST $\times$ SWC function) also increased the predictive ability as compared to Eq. (1) (only ST exponential function), increasing the $R^2$ from 0.71 to 0.78.

• 한국식품과학회지
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• 제35권3호
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• pp.470-474
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• 2003

식중독은 세균에 의한 발병이 대부분이다. 따라서 식품에서 식중독 원인균을 신속하게 탐색하게 식중독으로부터의 되면 피해를 줄일 수 있을 것이다. 고전적인 식중독 원인균 탐색은 증균, 선택적 배지를 이용한 isolation, 생화학적 특징을 활용하는 분석이 있으나 많은 시간이 소요되는 단점을 갖고 있었다. 본 연구는 16S rRNA gene(16S rDNA)로부터 얻은 DNA 염기 서열을 이용 식중독 원인균의 특이적 oligonucleotide probe을 제작 reverse dot blot hybridization과 PCR 방법을 이용하여 고전적인 방법보다 빠른 시간 내에 식품에서 원인균을 탐색 할 수 있었다. 우유를 인공적으로 본 연구에서 사용한 균주로 오염시킨 후 DNA를 추출하여 PCR 증폭산물과 oligonucleotide probe를 hybridization 시킨 결과 oligonucleotide probe를 hybridization 시킨 결과 oligonucleotide probe가 위치한 곳에서 발색 반응이 나타났다. 본 연구에서 본 연구를 통해 DNA microchip으로 활용 짧은 시간 내에 많은 종류의 식중독 원인균을 탐색 할 수 있는 가능성을 확인하였다.