• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2006년도 춘계학술대회 학술발표 논문집 제16권 제1호
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• pp.199-202
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• 2006

We propose some properties of fuzzy p-value and fuzzy significance level to the test statistics for the fuzzy hypotheses testing. Appling the principle of agreement index, we suggest two method for fuzzy hypothesis testing by fuzzy rejection region and fuzzy p-value with fuzzy hypothesis $H_{f,0}$.

• Journal of Plant Biology
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• 제32권1호
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• pp.23-32
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• 1989

We have developed a plasmid vector, pKCH1, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning sites, Bam HI and Xba I, between them. After inserting a foreing gene at the cloning sites, P35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind III.

• Archives of Pharmacal Research
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• 제25권2호
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• pp.208-213
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• 2002

The human polyomavirus JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML). As the JC virus early promoter directs cell-specific expression of the viral replication factor large T antigen, transcriptional regulation constitutes a major mechanism of glial tropism in PML. It has been demonstrated that SV4O or JC virus large T antigen interacts with p53 protein and regulates many viral and cellular genes. In this study we founts that p53 represses the JC virus early promoter in both glial and nonglial cells To identify the cis-regulatory elements responsible for p53-mediated repression, deletional and site-directed mutational analyses were performed . Deletion of the enhancer region diminished p53-mediated transcriptional repression. However, point mutations of several transcription factor binding sites in the basal promoter region did not produce any significant changes. In support of this observation, when the enhancer was fused to a heterologous promoter, p53 red reduced the promoter activity about three fold. These results indicate that the enhancer region is important for tole repression of JC virus transcription by p53. Furthermore, coexpression of JC virus T antigen with a p53 protein abolished p53-mediated repression of the JC virus early promoter in non-glial cells, but not in glial cells. This finding suggests that T antigen interacts with p53 and regulates JC virus transcription in a cell-specific manner.

• 한국초지조사료학회지
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• 제12권3호
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• pp.145-152
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• 1992

Phosphorus(P) cycle of Miscanthus sinensis, Sasa palmata and Artemicia princeps for perennial plants and Polygonwn thunbergii for annual plant were investigated in natural plant communities established in Andosol region. 1. Perennial plants contained about 50-80 % P of the maximum accumulated in whole plant in under ground parts as reserve P before wintering. On the other hand, annual plant returned the most part of P in plant tissues to soil and wintered with a small amount of P in only seed. 2. The storage percentage of K was low compared to P in both perennial and annual plants. And the return of K from litter to soil proceeded rapidly because of easy leaching of K. 3. Perennial plants stored a large amount of P in reserve organs before wintering and carry it over next generation, and P holded in an individual showed a yearly increase. Consequently, P was less susceptible to depressive factor for growth of perennial plants even in poor P circumstances like Andosol region, compared to annual plant.

• 한국멀티미디어학회논문지
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• 제13권1호
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• pp.38-46
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• 2010

P2P온라인 게임 시스템에서 대규모 사용자의 동시 접속을 수용할 수 있는 안전한 게임 운영 시스템이 필수적이다. 본 논문에서 제안하는 P2P 온라인 게임 시스템은 RS(Region Sever)들의 재구성 및 RS간의 숭호 정보 교환을 통해 한 영역에 플레이어가 집중되는 현상을 피하여 대규모 플레이어를 수용할 수 있으며, 안전한 게임을 운영할 수 있다. 또한 모니터링 서버의 광역 버퍼(Global Zone Buffer)를 이용한 부하분산으로 타임스탬프 시간 내의 게임 동기화가 가능하며고 미들웨어를 단위 영역별로 관리하여 게임 월드의 크기에 관계없이 수행 가능하다. 따라서, 서버 추가 문제의 해결 및 메시지 전송의 안정성을 확보할 수 있다. 또한, 시뮬레이션을 통하여 제안 시스템에 대한 효율성을 입증한다.

• 한국인터넷방송통신학회논문지
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• 제22권3호
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• pp.83-91
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• 2022

본 논문은 영역 별로 부하가 관리되는 MMOG를 위해서 확장가능한 하이브리드 P2P 클라우드 구조를 제시한다. 게임 세계는 여러 게임 영역으로 분할되며 각 게임 영역은 이러한 MMOG 클라우드 환경에서 최소 한 개 이상의 피어 즉 플레이어에 의해 서비스된다. 특정 영역에 플레이어들의 수가 급증한 경우에도 그들의 상호 작용이 원활하게 지원되도록 부하는 영역별로 관리되어야 한다. 제시된 구조에서 자원 공급이 효율적으로 실현되며 플레이어들은 클라우드 서버와 효과적으로 상호 작용할 수 있고 기존의 클라이언트 서버 MMOG 구조에서의 병목 현상을 회피할 수 있다. 이 구조는 플레이어들의 처리 능력을 활용함으로써 클라우드에 있는 서버의 부하 즉 컴퓨팅 능력과 통신량을 절감하게 된다. 시뮬레이션 결과 제시된 하이브리드 P2P 클라우드 구조는 혼잡 지역 또는 핫스팟에서 플레이어들의 수에 따라 이용가능한 자원도 같이 증가됨에 따라 클라이언트 서버 구조에 비하여 서버의 통신 대역폭을 상당부분 감소시킬 수 있는 것으로 나타났다.

• The Plant Pathology Journal
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• 제35권3호
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• pp.219-233
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• 2019

Soybean cultivars susceptible to Phytophthora root and stem rot are vulnerable to seed rot and damping-off of seedlings and young plants following an infection by Phytophthora sojae. In this study, the disease responses of Japanese soybean cultivars including currently grown main cultivars during the early growth stages were investigated following infections by multiple P. sojae isolates from Japanese fields. The extent of the resistance to 17 P. sojae isolates after inoculations at 14, 21, and 28 days after seeding varied significantly among 18 Japanese and two US soybean cultivars. Moreover, the disease responses of each cultivar differed significantly depending on the P. sojae isolate and the plant age at inoculation. Additionally, the treatment of 'Nattosyo-ryu' seeds with three fungicidal agrochemicals provided significant protection from P. sojae when plants were inoculated at 14-28 days after seeding. These results indicate that none of the Japanese soybean cultivars are completely resistant to all tested P. sojae isolates during the first month after sowing. However, the severity of the disease was limited when plants were inoculated during the later growth stages. Furthermore, the protective effects of the tested agrochemicals were maintained for at least 28 days after the seed treatment. Japanese soybean cultivars susceptible to Phytophthora root and stem rot that are grown under environmental conditions favorable for P. sojae infections require the implementation of certain practices, such as seed treatments with appropriate agrochemicals, to ensure they are protected from P. sojae during the early part of the soybean growing season.

• BMB Reports
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• 제29권1호
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• pp.92-97
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• 1996

The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52~-37 bp from the anaerobic mRNA 5'-end, which is the binding site of NarL and NarP. The other is located at +37~+56 bp from the 5'-end of mRNA. In this study, the downstream symmetry region from the mRNA 5'-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.

• The Journal of Korean Physical Therapy
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• 제10권1호
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• pp.53-65
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• 1998

The main purpose of this paper is to analyze the modes of therapeutic intervention. The emphasis is on the neurophysiological perpective arising out of neurological principles and developmental concepts. The obtained results are as follows. 1. The important hypostheses predicted that the group intervened by neurodevelopmental approach would improve motor function better than the group done by traditional approach and it was proved that neurodevelopmental approach was more effective in gross motor region(P<.01) 2. In the comparison of type of involovement, neurodevelopmental intervention group in spastic type showed improvements in the region of gross motor.(P<.001) 3. In the comparision of degree of disorder, neurodevelopmental intervention group showed improvement of motor function in all the gross motor region in the mild, moderate and severe case.(P<.001) 4. In the comparison of ages of intervention beginning, the group of child between 25-36, 49-60 and 61-72 months(P<.001) intervened by neurodevelopmental approach showed improvements of motor function. 5. In the comparison of intervention duration, neurodevelopmental intervention group showed improvements of motor function in gross motor region according to intervention durstion(P<.001)

• BMB Reports
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• 제39권1호
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• pp.61-67
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• 2006

Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.