• 한국환경보건학회지
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• 제26권4호
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• pp.88-96
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• 2000

The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4',4-diisothiocyano-2',2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the $Ca^{2+}$ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.

• 한국미생물·생명공학회지
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• 제26권5호
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• pp.435-441
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• 1998

Red beet(Beta vulagris L.) 모상근의 회분배양을 이용한 천연색소인 betacyanin 생산 최적화를 위해 광도, C/N ratio, 인산의 농도를 각각 변화시켜 세포생육과 색소 생산성에 관한 동력학적 분석을 실시했다. 광도변화에 따른 배양 결과 3 klux의 경우 0.3(1/day)의 최대 비 생육속도와 0.11(mg/g-dry cell/day)의 최대 비 생산속도 그리고 14 k1ux에서 0.242(1/day)의 최대 비 생육속도와 0.125(mg/g-dry cell/day)의 최대 비 생산속도를 나타냈다. 광도와 균체의 생육관계를 검토한 결과 광도에 따른 세포 생육은 photoinhibition model이 적용됨이 확인되었다. Red beet 모상근으로부터 betacyanin의 생산은 partially growth related process임이 입증됐다. 이에 따른 세포당 최대 betacyanin 생산을 나타내는 $\alpha$는 0.3756 (mg/cell)이며, 최대 생산속도를 나타내는 $\beta$는 0.001 (mg/g-cel1/day)로 측정됐다. C/N ratio에 따른 실험결과 42.1(w/w)에서 0.26(1/day)의 최대 비생육 속도를 나타내었으나 최대 비 생산속도는 31.6(w/w)에서 0.075(mg/g-cell/day)를 나타냈다. 인 농도에 대한 균체의 생육 및 물질 생산성의 관계를 검토한 결과 1.25mM에서 0.31(1/day)의 비생육 속도와 0.134(mg/g-dry cell/day) 비생산 속도를 나타내었다. 최적 조건을 결정하기 위한 response surface methodology(RSM)결과 세포 생육과 betacyanin의 최대 생산을 위한 최적 광도는 5.5 (klux),최적 C/N ratio와 인의 농도는 27(w/w), 1.25 (mM)로 결정됐다. 그리고 0.1 $\mu$M kinetin 첨가시 대조구에 비해 비생산성이 0.085(mg/g-dry cell/day)로 증가함이 입증됐다. Normal조건과 optimum조건의 비교결과 세포의 농도인 X(g-dry wt./L)가 8와 16, betacyanin의 생산량인 P(mg/L)가 4.48과 12.5, 그리고 optimum 조건에서 최대비 생육속도인 $\mu$$_{max}$ 가 0.375와 그리고 최대비 생산속도인 q$^{max}$ $_{p}$ 는 0.134로 약 2배로서 최적화가 되었다.

• 헤리티지:역사와 과학
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• 제46권3호
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• pp.172-193
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• 2013

본 논문은 도쿄 오쿠라슈코칸(東京 大倉集古館)에 소장되어 있는 고려 후기의 협저보살좌상(夾紵菩薩坐像)의 연구를 통하여 한국 협저불상에 나타나는 제작 기법과 재료에 관하여 고찰한 것이다. 연구방법으로는 먼저, X-ray 데이터와 현장조사결과를 종합하여 이 상에 나타나는 제작기법과 재료의 특징을 분석하였다. 이것을 기초로 제작과정에 대한 가설을 세운 다음, 검증을 위하여 실제 제작과정을 재연하고 각 공정에 적용된 기법의 특징과 재료에 대하여 상세히 서술하였다. 특히, 협저기법과 재료 부분에 있어서는 육안관찰 결과를 토대로 문헌자료와 비교 검토하며 추정하였는데, 칠예기법의 교과서격인 명대(明代) 황성(黃成)이 편찬한 "휴식록(?飾?)"의 기록을 중심으로 적용하였으며, 한국의 전통나전칠기 제작기법에 사용되고 있는 재료도 참고하였다. 그 결과, 오쿠라슈코칸상에 나타나고 있는 제작기법과 재료의 특징은 호놀롤루 미술관(Honolulu Museum of Art)에 소장되어 있는 송대(宋代) 원부2년명(元符二年銘 1098) 협저나한좌상(夾紵羅漢坐像)과 많은 부분에서 유사성이 있음을 알 수 있었다. 특히, 원형(原型)의 흙과 심목을 제거한 후 새로 심목을 제작하여 넣지 않아 상의 내부가 텅 비어 있는 점이나, 소상(塑像)의 단계에서 완전한 형태를 만들어 베 바르기를 하는 점, 소상의 흙과 심목은 상의 아랫부분을 통해서 제거하는 점, 모델링(Modelling) 작업 단계를 거치지 않으며 마감층이 매우 얇다는 점 등이 공통된 특징이다. 그 외에도 보발과 영락 등 장식물을 가소성 재료로 따로 만들어 상을 장엄하는 것은 송대에 크게 유행하였던 기법으로, 오쿠라슈코칸상이 송대의 협저불 제작기법으로부터 많은 영향을 받았을 가능성에 대해 시사한다. 한국 협저불상에 대해서는 기원과 역사에 관한 명확한 기록이 전하지 않으며, 현존하는 작품도 매우 한정되어 있다. 원풍연간(元豊年間 1078~1085)의 협저불상 전래를 알 수 있는 "선화봉사고려도경(宣和奉使高麗圖經)"의 기록도 최초의 전래시기로는 보기 힘들다. 또한 현재 남아 있는 협저불상들을 통해 제작기법의 전래경로를 명백하게 찾아내기도 그리 쉬운 상황만은 아니다. 그러나 본 연구를 통해 오쿠라슈코칸상을 위시한 현존하는 한국의 협저불상들은 적어도, 협저불상 제작기법의 정설처럼 알려져 있던 기존의 일본 탈활협저불상의 제작기법과는 많은 차이점이 있음을 밝혀낼 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권7호
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• pp.1048-1053
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• 2009

The present study was conducted to determine the effects of chitosan on nitric oxide (NO) content and inducible nitric oxide synthase (iNOS) activity in serum, and relative expression of iNOS mRNA in the duodenum, jejunum, and ileum of broiler chickens. A total of 240 one-day-old Arbor Acre mixed-sex broiler chickens were randomly allotted to six dietary treatments with five replicates in each treatment and eight chickens in each replicate. The broiler chickens in the six treatments were fed the basal diet supplemented with 0 (control), 0.05, 0.2, 0.5, 1.0 or 2.0 g/kg chitosan. The trial lasted for 42 days. The results showed that dietary chitosan enhanced NO content and iNOS activity in serum as well as iNOS mRNA expression in the duodenum and ileum of broiler chickens in a quadratic dose-dependent manner (p<0.05), and improved jejunum iNOS mRNA expression in a quadratic dose-dependent manner (p<0.10) with increasing addition of chitosan. Chicks fed a diet containing 0.5-1.0 g/kg chitosan had higher NO content and iNOS activity in serum as well as small-intestinal iNOS mRNA expression compared with birds given the control diet, but positive effects of chitosan tended to be suppressed when addition of chitosan in the diet was increased to 2.0 g/kg. These results implied that there was a threshold level of chitosan inclusion beyond which progressive reductions in serum NO content and small intestinal iNOS expression occured, and the regulation of chitosan on immune functions in chickens is probably associated with activated expression of iNOS and NO secretion.

• Asian-Australasian Journal of Animal Sciences
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• 제13권1호
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• pp.46-52
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• 2000

Two experiments were conducted to determine ileal digestibilities for the amino acids contained in rapeseed meal using the regression technique and then applying the values obtained, in a growth trial, using growing-finishing pigs. For the digestibility trial, four 20 kg crossbred $(Yorkshire{\times}Landrace{\times}Beijing\;Black)$ barrows were fitted with simple T-cannula in the terminal ileum. After recovery, the barrows were fed one of four experimental diets according to a $4{\times}4$ Latin Square design. The pigs were fed corn-soybean meal based diets supplemented with 0, 25, 50 or 75% rapeseed meal. For the growth trial, 80 crossbred $(Yorkshire{\times}Landrace{\times}Beijing\;Black)$ growing pigs $(20{\pm}2.4kg)$ were fed corn-soybean meal diets supplemented with 0, 3, 6, 9 or 12% rapeseed meal. Four pens (2 gilts and 2 castrates) were assigned to each treatment. With the exception of isoleucine and methionine, the digestibility coefficients for the indispensible amino acids declined as the level of rapeseed meal in the diet increased. There was little agreement between the amino acid digestibilities determined with the regression technique and values previously published for rapeseed meal. During the growing (22-42 kg) period, the addition of rapeseed meal had no significant effects on gain, feed intake or feed conversion. During the finishing period (58-91 kg), daily gain was not affected by rapeseed meal inclusion but feed conversion declined (p<0.04) as the level of rapeseed meal in the diet increased.

• 한국토양비료학회지
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• 제49권6호
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• pp.635-643
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• 2016

Wine consumers desire to drink a high quality wine. For producing high quality wine, high quality soil is required. Conventionally, soil quality is assessed qualitatively. Using traditional laboratory methods, quantitative data can be obtained for management purpose, but it is time consuming and expensive. Therefore, new technology aims to address these limitations, namely portable X-Ray fluorescence spectrometers (pXRF). This instrument can be used directly in the field, requires no soil sample preparations, and can simultaneously measure a wide range of elements qualitatively that are useful for pedological studies. The chemical composition (Ca, Fe, Ti and Zr) of soils at Tallavera Grove vineyard in New South Wales, Australia, was studied using a pXRF. The analysis of the soil's elemental concentration (i.e. Ca and Fe) using pXRF supports management decisions. Measuring the soil's Ca concentration can be used to identify Ca-rich parent materials (limestone). The limestone indicates good soil conditions for vine production. Fe content was used to identify areas of texture-contrast soils or soil with accumulation of clays in the B horizon. In addition, a soil weathering index was calculated using elemental concentrations (i.e. Ti and Zr) to explore the history of soil formation for making decision of management. This index showed that the soil in the vineyard was affected by two processes: the deposition of materials from elsewhere (Aeolian transport or soil erosion) and mixing of materials from upslope.

• 대한디지털의료영상학회논문지
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• 제9권2호
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• pp.5-9
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• 2007

As developing the medical treatment image portion with the change of these times, PACS, which is able to digitalize image portion data, has a lot of data-based image data. Applying this PACS, we would like to settle down RNSXI(real-name shooting X -ray of inspector) system. We interviewed with P ACS's operators of university hospitals which is using PACS in Seoul about the present conditions whether using of RNSXI or not. And we inquired the RNSXI equipments, applying PACS database, and Interface conditions undertook to do in our hospital. All university hospitals in Seoul are set up the P ACS system. But no hospital use the RNSXI. In our hospital, we can check inspector' name or initials who exposure x-ray with the PACS Viewer by looking over equipments(CR, DR, US, MG, MR, CT) and Interface of the DICOM Header data. However, some equipments like RF and Angio can not check inspector' name or initials. Under the Film/System environment, RNSXI system has been used frequently like that inspector's signature or initial added to a patient data. Though the digital medical treatment was developed, RNSXI system was declined. It is necessary to using RNSXI system in order to improving radiologists' rights, even if it is not under the application of the medical treatment image laws. If RNSXI system use, radiologists should specialize in their major and the Repeat rate should be reduced. In environment of PACS, RNSXI system can be used by linking both the equipments and the Interface with a production enterprise of P ACS. Therefore RNSXI system applying the P ACS datebase should settle down in our medical system for being provided lots of data.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1791-1797
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• 2007

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

• 한국가축번식학회지
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• 제25권2호
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• pp.171-180
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• 2001

형질전환 가금의 생산에 있어서 retrovirus vector를 이용하는 방법은 다양한 종류의 표적세포에 대하여 retrovirus 고유의 감염성에 의한 외래 유전자의 전이가 용이하고, 전이된 유전자가 진정염색질 영역 내로 선택적으로 도입될 수 있으며 유전적으로 안정성을 나타내므로 매우 효과적인 방법이다. 그러나 가금에서는 초기 배발달에 의한 급격한 세포의 수적 증가로 인해 고감염성의 virus의 획득이 요구되므로, 이를 위하여 virus stock의 농축에 있어 보다 안정적이고 pantropic인 vesicular stomatitis virus (VSV G) glycoprotein를 envelope로 가지는 pseudotyped retrovirus vector system을 이용하였으며, marker gene으로 eGFP gene이 발현되는 retrovirus를 생산하였다. 이 virus를 이용하여 여러 가지 표적세포와 primary culture한 CEF세포를 감염시켜 GFP의 발현을 확인하였으며, 농축한 virus stock은 stage X의 계란을 선택하여 windowed egg를 제작한 후 배하층에 주입하였다. 형질전환 닭은 정상 발생한 닭에 비하여 저조한 발생율을 보였으나 PCR을 이용하여 외래 유전자의 도입을 확인한 결과 100%인 것으로 나타났다. 또한 한 개체 내에서 유전자의 도입이 폐, 간, 정소, 소장 등의 여러 장기에서 확인되었다.

• 농업과학연구
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• 제46권3호
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• pp.497-506
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• 2019

The aim of this study was to estimate the factors that can be used as a predictive indicator of antler productivity in elk. Body weight and antler data from 115 stags raised at the Animal Genetic Resources Research Center in Korea were used in this study. The function for the average antler weight was derived by age, which was $y=-3.4693+5.3747x-0.8x^2+0.0424x^3$, and $R^2$ was 0.991. The analysis of the relationship between body weight at birth and the 1st and 2nd year and the antler weight after the 2nd year showed a correlation between them. There was a correlation between the antler weight of 1 and 2 year old stags and the weight of 2 year old stags as well; especially, both antler weights for the 1st and 2nd year were highly correlated with the antler weight of the 4th year. However, there was no relationship between the antler main beam length for the 1st and 2nd year and the antler weight after the 3rd year. Therefore, it is expected that a large amount of antler will be obtained in the future if proper management is carried out for young deer from birth to 2 years old. Additionally, the results of this study are expected to be useful for the development of a deer breeding program and the deer industry.