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http://dx.doi.org/10.5713/ajas.2007.1791

Characterization of BoLA-DRB3.2 Alleles in Hanwoo (Korean cattle) by Sequence Based Typing (SBT)  

Jeong, H.J. (Division of Animal Science and Resources, Chungnam National University)
Bhuiyan, M.S.A. (Division of Animal Science and Resources, Chungnam National University)
Lee, J.S. (Division of Animal Science and Resources, Chungnam National University)
Yu, S.L. (Division of Animal Science and Resources, Chungnam National University)
Sang, B.C. (Division of Animal Science and Resources, Chungnam National University)
Yoon, D. (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science)
Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
Lee, J.H. (Division of Animal Science and Resources, Chungnam National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.12, 2007 , pp. 1791-1797 More about this Journal
Abstract
A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.
Keywords
BoLA-DRB3; PCR-RFLP; Sequence Based Typing (SBT); Hanwoo;
Citations & Related Records
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